TY  - JOUR
AU  - Calic, Dusica D
AU  - Bohanec, Borut
AU  - Devrnja, Nina M
AU  - Milojević, Jelena D
AU  - Tubic, Ljiljana
AU  - Kostić, Igor
AU  - Zdravkovic-Korac, Snežana R
PY  - 2013
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/701
AB  - Horse chestnut (Aesculus hyppocastanum L., Hyppocastanacea) is a relict species with a slow and complex reproductive cycle considered to have horticultural and medical importance. The cycle maybe circumvented via in vitro androgenesis. Androgenesis of horse chestnut was induced in microspores and anther culture on MS media. Some of the horse chestnut androgenic embryos were albinos. Addition of abscisic acid in media (in concentrations of 0.01, 0.1, 0.5, 1, 2, 5, 10, and 20 mg l(-1)) with horse chestnut androgenic embryos has circumvented the reproduction cycle barriers. The best results were achieved on medium with the lowest abscisic acid concentration (0.01 mg l(-1)) in microspore culture. The microspore culture proved to be a better model system for embryo production and albino embryo reduction than anther culture. Flow cytometry analysis after maturation treatments induced by ABA showed that 88 % of green embryos originating from microspore culture were haploid. However, 50 % of green embryos from anther culture were haploid. The remaining analyzed androgenic embryos, from both types of cultures were diploid.
PB  - Springer Heidelberg, Heidelberg
T2  - Trees-Structure and Function
T1  - Impact of abscisic acid in overcoming the problem of albinism in horse chestnut androgenic embryos
EP  - 762
IS  - 3
SP  - 755
VL  - 27
DO  - 10.1007/s00468-012-0830-4
ER  - 

@article{
author = "Calic, Dusica D and Bohanec, Borut and Devrnja, Nina M and Milojević, Jelena D and Tubic, Ljiljana and Kostić, Igor and Zdravkovic-Korac, Snežana R",
year = "2013",
abstract = "Horse chestnut (Aesculus hyppocastanum L., Hyppocastanacea) is a relict species with a slow and complex reproductive cycle considered to have horticultural and medical importance. The cycle maybe circumvented via in vitro androgenesis. Androgenesis of horse chestnut was induced in microspores and anther culture on MS media. Some of the horse chestnut androgenic embryos were albinos. Addition of abscisic acid in media (in concentrations of 0.01, 0.1, 0.5, 1, 2, 5, 10, and 20 mg l(-1)) with horse chestnut androgenic embryos has circumvented the reproduction cycle barriers. The best results were achieved on medium with the lowest abscisic acid concentration (0.01 mg l(-1)) in microspore culture. The microspore culture proved to be a better model system for embryo production and albino embryo reduction than anther culture. Flow cytometry analysis after maturation treatments induced by ABA showed that 88 % of green embryos originating from microspore culture were haploid. However, 50 % of green embryos from anther culture were haploid. The remaining analyzed androgenic embryos, from both types of cultures were diploid.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Trees-Structure and Function",
title = "Impact of abscisic acid in overcoming the problem of albinism in horse chestnut androgenic embryos",
pages = "762-755",
number = "3",
volume = "27",
doi = "10.1007/s00468-012-0830-4"
}

Calic, D. D., Bohanec, B., Devrnja, N. M., Milojević, J. D., Tubic, L., Kostić, I.,& Zdravkovic-Korac, S. R.. (2013). Impact of abscisic acid in overcoming the problem of albinism in horse chestnut androgenic embryos. in Trees-Structure and Function
Springer Heidelberg, Heidelberg., 27(3), 755-762.
https://doi.org/10.1007/s00468-012-0830-4

Calic DD, Bohanec B, Devrnja NM, Milojević JD, Tubic L, Kostić I, Zdravkovic-Korac SR. Impact of abscisic acid in overcoming the problem of albinism in horse chestnut androgenic embryos. in Trees-Structure and Function. 2013;27(3):755-762.
doi:10.1007/s00468-012-0830-4 .

Calic, Dusica D, Bohanec, Borut, Devrnja, Nina M, Milojević, Jelena D, Tubic, Ljiljana, Kostić, Igor, Zdravkovic-Korac, Snežana R, "Impact of abscisic acid in overcoming the problem of albinism in horse chestnut androgenic embryos" in Trees-Structure and Function, 27, no. 3 (2013):755-762,
https://doi.org/10.1007/s00468-012-0830-4 . .

TY  - CONF
AU  - Zdravković-Korać, Snežana
AU  - Tubić, Ljiljana
AU  - Milojević, Jelena D
AU  - Devrnja, Nina
AU  - Kostić, Igor
AU  - Ćalić, Dušica
PY  - 2012
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2887
AB  - Efficient bud regeneration was achieved from germinating horse chestnut (Aesculus hippocastanum L.) somatic embryos cultivated on 1-10 μM benzyladenine (BA). Adventitious buds were detached from the mother tissue and used to establish permanent shoot cultures on 0-20 μM BA. Secondary buds were regenerated from the shoot base of the explants. Bud multiplication was very poor (1.9) and shoot-tip necrosis was very high (100%) on plant growth regulator (PGR)-free medium. The highest multiplication was achieved on 5 and 10 μM BA (16.8 and 18.7, respectively), with no shoot-tip necrosis, while hyperhydration was rather frequent on shoots cultivated on BA above 5 μM. Individual shoots were elongated on medium with 1 μM BA and 500 mg/l polyvinylpyrrolidone (PVP MW40 000) for 4 weeks. However, it was necessary to reduce BA level below 1 μM for shoot rooting and that caused mass shoot-tip necrosis. As classical rooting methods failed, the basal part of each elongated shoot was first wounded by cutting with a sterile blade and then dipped into a 0, 5 or 10 mM indole-3-butyric acid solution for 1 min and cultivated on solid half-strength MS PGR-free medium with 0.02% activated charcoal for 2-3 weeks. To prevent shoot tip necrosis during this phase, a BA solution was applied directly on apical meristem. Shoot-tip necrosis was completely eliminated by weekly application of 10 μl of 1 μM BA. As soon as the root initials were observed, the shoots were transferred to MS medium supplemented with 500 mg/l PVP and 5 μM BA. The frequency of rooting was 23%, and further optimisation of root-inducing phase is needed.
PB  - Belgrade: Institute of Forestry
C3  - Proceedings: International Scientific Conference: Forest in the Future - Sustainable Use, Risks and Challenges; 2012 Oct 4-5; Belgrade, Serbia.
T1  - Rooting and preventing shoot-tip necrosis of in vitro cultured horse chestnut shoots
EP  - 396
SP  - 389
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2887
ER  - 

@conference{
author = "Zdravković-Korać, Snežana and Tubić, Ljiljana and Milojević, Jelena D and Devrnja, Nina and Kostić, Igor and Ćalić, Dušica",
year = "2012",
abstract = "Efficient bud regeneration was achieved from germinating horse chestnut (Aesculus hippocastanum L.) somatic embryos cultivated on 1-10 μM benzyladenine (BA). Adventitious buds were detached from the mother tissue and used to establish permanent shoot cultures on 0-20 μM BA. Secondary buds were regenerated from the shoot base of the explants. Bud multiplication was very poor (1.9) and shoot-tip necrosis was very high (100%) on plant growth regulator (PGR)-free medium. The highest multiplication was achieved on 5 and 10 μM BA (16.8 and 18.7, respectively), with no shoot-tip necrosis, while hyperhydration was rather frequent on shoots cultivated on BA above 5 μM. Individual shoots were elongated on medium with 1 μM BA and 500 mg/l polyvinylpyrrolidone (PVP MW40 000) for 4 weeks. However, it was necessary to reduce BA level below 1 μM for shoot rooting and that caused mass shoot-tip necrosis. As classical rooting methods failed, the basal part of each elongated shoot was first wounded by cutting with a sterile blade and then dipped into a 0, 5 or 10 mM indole-3-butyric acid solution for 1 min and cultivated on solid half-strength MS PGR-free medium with 0.02% activated charcoal for 2-3 weeks. To prevent shoot tip necrosis during this phase, a BA solution was applied directly on apical meristem. Shoot-tip necrosis was completely eliminated by weekly application of 10 μl of 1 μM BA. As soon as the root initials were observed, the shoots were transferred to MS medium supplemented with 500 mg/l PVP and 5 μM BA. The frequency of rooting was 23%, and further optimisation of root-inducing phase is needed.",
publisher = "Belgrade: Institute of Forestry",
journal = "Proceedings: International Scientific Conference: Forest in the Future - Sustainable Use, Risks and Challenges; 2012 Oct 4-5; Belgrade, Serbia.",
title = "Rooting and preventing shoot-tip necrosis of in vitro cultured horse chestnut shoots",
pages = "396-389",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2887"
}

Zdravković-Korać, S., Tubić, L., Milojević, J. D., Devrnja, N., Kostić, I.,& Ćalić, D.. (2012). Rooting and preventing shoot-tip necrosis of in vitro cultured horse chestnut shoots. in Proceedings: International Scientific Conference: Forest in the Future - Sustainable Use, Risks and Challenges; 2012 Oct 4-5; Belgrade, Serbia.
Belgrade: Institute of Forestry., 389-396.
https://hdl.handle.net/21.15107/rcub_rimsi_2887

Zdravković-Korać S, Tubić L, Milojević JD, Devrnja N, Kostić I, Ćalić D. Rooting and preventing shoot-tip necrosis of in vitro cultured horse chestnut shoots. in Proceedings: International Scientific Conference: Forest in the Future - Sustainable Use, Risks and Challenges; 2012 Oct 4-5; Belgrade, Serbia.. 2012;:389-396.
https://hdl.handle.net/21.15107/rcub_rimsi_2887 .

Zdravković-Korać, Snežana, Tubić, Ljiljana, Milojević, Jelena D, Devrnja, Nina, Kostić, Igor, Ćalić, Dušica, "Rooting and preventing shoot-tip necrosis of in vitro cultured horse chestnut shoots" in Proceedings: International Scientific Conference: Forest in the Future - Sustainable Use, Risks and Challenges; 2012 Oct 4-5; Belgrade, Serbia. (2012):389-396,
https://hdl.handle.net/21.15107/rcub_rimsi_2887 .

TY  - JOUR
AU  - Calic, Dusica D
AU  - Devrnja, Nina M
AU  - Milojević, Jelena D
AU  - Kostić, Igor
AU  - Janosević, Dusica A
AU  - Budimir, Snežana M
AU  - Zdravkovic-Korac, Snežana R
PY  - 2012
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/583
AB  - The effect of abscisic acid on the development of primary androgenic embryo and secondary somatic embryogenesis was investigated with the aim of improving multiplication rates and secondary somatic embryo quality in horse chestnut microspore and anther culture. The early embryo stage (globular) had a better response than late stages (heart, torpedo, and cotyledonary) in both types of cultures. Also, microspore culture had a high potential for mass secondary embryo production. The number of secondary somatic embryos was three times higher on hormone-free medium than on medium enriched with 0.01 mg.L-1 abscisic acid. However, most of the embryos on hormone-free medium had abnormal morphology. For this reason, abscisic acid was added to the media to improve embryo quality. The morphology of abscisic acid treated embryos was better than abscisic acid non-treated embryos. The optimal abscisic acid concentration for secondary somatic embryo induction and production of high-quality embryos was 0.01 mg.L-1. Overall, the effect of abscisic acid on the induction of secondary somatic embryogenesis and plant regeneration of androgenic embryos of this species may be helpful for the further synthesis of secondary metabolites in vitro and their application in the pharmaceutical industry.
PB  - Amer Soc Horticultural Science, Alexandria
T2  - Hortscience
T1  - Abscisic Acid Effect on Improving Horse Chestnut Secondary Somatic Embryogenesis
EP  - 1744
IS  - 12
SP  - 1741
VL  - 47
DO  - 10.21273/HORTSCI.47.12.1741
ER  - 

@article{
author = "Calic, Dusica D and Devrnja, Nina M and Milojević, Jelena D and Kostić, Igor and Janosević, Dusica A and Budimir, Snežana M and Zdravkovic-Korac, Snežana R",
year = "2012",
abstract = "The effect of abscisic acid on the development of primary androgenic embryo and secondary somatic embryogenesis was investigated with the aim of improving multiplication rates and secondary somatic embryo quality in horse chestnut microspore and anther culture. The early embryo stage (globular) had a better response than late stages (heart, torpedo, and cotyledonary) in both types of cultures. Also, microspore culture had a high potential for mass secondary embryo production. The number of secondary somatic embryos was three times higher on hormone-free medium than on medium enriched with 0.01 mg.L-1 abscisic acid. However, most of the embryos on hormone-free medium had abnormal morphology. For this reason, abscisic acid was added to the media to improve embryo quality. The morphology of abscisic acid treated embryos was better than abscisic acid non-treated embryos. The optimal abscisic acid concentration for secondary somatic embryo induction and production of high-quality embryos was 0.01 mg.L-1. Overall, the effect of abscisic acid on the induction of secondary somatic embryogenesis and plant regeneration of androgenic embryos of this species may be helpful for the further synthesis of secondary metabolites in vitro and their application in the pharmaceutical industry.",
publisher = "Amer Soc Horticultural Science, Alexandria",
journal = "Hortscience",
title = "Abscisic Acid Effect on Improving Horse Chestnut Secondary Somatic Embryogenesis",
pages = "1744-1741",
number = "12",
volume = "47",
doi = "10.21273/HORTSCI.47.12.1741"
}

Calic, D. D., Devrnja, N. M., Milojević, J. D., Kostić, I., Janosević, D. A., Budimir, S. M.,& Zdravkovic-Korac, S. R.. (2012). Abscisic Acid Effect on Improving Horse Chestnut Secondary Somatic Embryogenesis. in Hortscience
Amer Soc Horticultural Science, Alexandria., 47(12), 1741-1744.
https://doi.org/10.21273/HORTSCI.47.12.1741

Calic DD, Devrnja NM, Milojević JD, Kostić I, Janosević DA, Budimir SM, Zdravkovic-Korac SR. Abscisic Acid Effect on Improving Horse Chestnut Secondary Somatic Embryogenesis. in Hortscience. 2012;47(12):1741-1744.
doi:10.21273/HORTSCI.47.12.1741 .

Calic, Dusica D, Devrnja, Nina M, Milojević, Jelena D, Kostić, Igor, Janosević, Dusica A, Budimir, Snežana M, Zdravkovic-Korac, Snežana R, "Abscisic Acid Effect on Improving Horse Chestnut Secondary Somatic Embryogenesis" in Hortscience, 47, no. 12 (2012):1741-1744,
https://doi.org/10.21273/HORTSCI.47.12.1741 . .