TY  - JOUR
AU  - Ducic, Tanja
AU  - Ninković, Milena
AU  - Martinez-Rovira, Immaculada
AU  - Sperling, Swetlana
AU  - Rohde, Veit
AU  - Dimitrijević, Dragoljub
AU  - Jover, Manas, Gabriel Vicent
AU  - Vaccari, Lisa
AU  - Birarda, Giovanni
AU  - Yousef, Ibraheem
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1540
AB  - Glioblastoma multiforme (GBM) is the most aggressive brain tumor, characterized by short median survival and an almost 100% tumor-related mortality. The standard of care treatment for newly diagnosed GBM includes surgical resection followed by concomitant radiochemotherapy. The prevention of disease progression fails due to the poor therapeutic effect caused by the great molecular heterogeneity of this tumor. Previously, we exploited synchrotron radiation-based soft X-ray tomography and hard X-ray fluorescence for elemental microimaging of the shock-frozen GBM cells. The present study focuses instead on the biochemical profiling of live GBM cells and provides new insight into tumor heterogenicity. We studied bio-macromolecular changes by exploring the live-cell synchrotron-based Fourier transform infrared (SR-FTIR) microspectroscopy in a set of three GBM cell lines, including the patient-derived glioblastoma cell line, before and after riluzole treatment, a medicament with potential anticancer properties. SR-FTIR microspectroscopy shows that GBM live cells of different origins recruit different organic compounds. The riluzole treatment of all GBM cell lines mainly affected carbohydrate metabolism and the DNA structure. Lipid structures and protein secondary conformation are affected as well by the riluzole treatment: cellular proteins assumed cross beta-sheet conformation while parallel beta-sheet conformation was less represented for all GBM cells. Moreover, we hope that a new live-cell approach for GBM simultaneous treatment and examination can be devised to target cancer cells more specifically, i.e., future therapies can develop more specific treatments according to the specific bio-macromolecular signature of each tumor type.
PB  - Amer Chemical Soc, Washington
T2  - Analytical Chemistry
T1  - Live-Cell Synchrotron-Based FTIR Evaluation of Metabolic Compounds in Brain Glioblastoma Cell Lines after Riluzole Treatment
EP  - 1940
IS  - 4
SP  - 1932
VL  - 94
DO  - 10.1021/acs.analchem.1c02076
ER  - 

@article{
author = "Ducic, Tanja and Ninković, Milena and Martinez-Rovira, Immaculada and Sperling, Swetlana and Rohde, Veit and Dimitrijević, Dragoljub and Jover, Manas, Gabriel Vicent and Vaccari, Lisa and Birarda, Giovanni and Yousef, Ibraheem",
year = "2022",
abstract = "Glioblastoma multiforme (GBM) is the most aggressive brain tumor, characterized by short median survival and an almost 100% tumor-related mortality. The standard of care treatment for newly diagnosed GBM includes surgical resection followed by concomitant radiochemotherapy. The prevention of disease progression fails due to the poor therapeutic effect caused by the great molecular heterogeneity of this tumor. Previously, we exploited synchrotron radiation-based soft X-ray tomography and hard X-ray fluorescence for elemental microimaging of the shock-frozen GBM cells. The present study focuses instead on the biochemical profiling of live GBM cells and provides new insight into tumor heterogenicity. We studied bio-macromolecular changes by exploring the live-cell synchrotron-based Fourier transform infrared (SR-FTIR) microspectroscopy in a set of three GBM cell lines, including the patient-derived glioblastoma cell line, before and after riluzole treatment, a medicament with potential anticancer properties. SR-FTIR microspectroscopy shows that GBM live cells of different origins recruit different organic compounds. The riluzole treatment of all GBM cell lines mainly affected carbohydrate metabolism and the DNA structure. Lipid structures and protein secondary conformation are affected as well by the riluzole treatment: cellular proteins assumed cross beta-sheet conformation while parallel beta-sheet conformation was less represented for all GBM cells. Moreover, we hope that a new live-cell approach for GBM simultaneous treatment and examination can be devised to target cancer cells more specifically, i.e., future therapies can develop more specific treatments according to the specific bio-macromolecular signature of each tumor type.",
publisher = "Amer Chemical Soc, Washington",
journal = "Analytical Chemistry",
title = "Live-Cell Synchrotron-Based FTIR Evaluation of Metabolic Compounds in Brain Glioblastoma Cell Lines after Riluzole Treatment",
pages = "1940-1932",
number = "4",
volume = "94",
doi = "10.1021/acs.analchem.1c02076"
}

Ducic, T., Ninković, M., Martinez-Rovira, I., Sperling, S., Rohde, V., Dimitrijević, D., Jover, M. G. V., Vaccari, L., Birarda, G.,& Yousef, I.. (2022). Live-Cell Synchrotron-Based FTIR Evaluation of Metabolic Compounds in Brain Glioblastoma Cell Lines after Riluzole Treatment. in Analytical Chemistry
Amer Chemical Soc, Washington., 94(4), 1932-1940.
https://doi.org/10.1021/acs.analchem.1c02076

Ducic T, Ninković M, Martinez-Rovira I, Sperling S, Rohde V, Dimitrijević D, Jover MGV, Vaccari L, Birarda G, Yousef I. Live-Cell Synchrotron-Based FTIR Evaluation of Metabolic Compounds in Brain Glioblastoma Cell Lines after Riluzole Treatment. in Analytical Chemistry. 2022;94(4):1932-1940.
doi:10.1021/acs.analchem.1c02076 .

Ducic, Tanja, Ninković, Milena, Martinez-Rovira, Immaculada, Sperling, Swetlana, Rohde, Veit, Dimitrijević, Dragoljub, Jover, Manas, Gabriel Vicent, Vaccari, Lisa, Birarda, Giovanni, Yousef, Ibraheem, "Live-Cell Synchrotron-Based FTIR Evaluation of Metabolic Compounds in Brain Glioblastoma Cell Lines after Riluzole Treatment" in Analytical Chemistry, 94, no. 4 (2022):1932-1940,
https://doi.org/10.1021/acs.analchem.1c02076 . .

TY  - JOUR
AU  - Ducic, Tanja
AU  - Milenković, Ivana
AU  - Mutavdžić, Dragosav
AU  - Nikolic, Miroslav
AU  - Martinez, de Yuso, M. Valle
AU  - Vučinić, Željko
AU  - Algarra, Manuel
AU  - Radotić, Ksenija
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1450
AB  - Carbon dots are biocompatible and non-toxic nanoparticles with chemical affinity to some heavy metals. Human activities increase soil pollution with copper. Cu is an essential microelement in plants, but excess can induce a harmful effects. In plant response to Cu, the cell wall plays an important role. This study aims to estimate possible amelioration effects of folic acid based CDs on Cu toxicity by studying the intracellular and cell wall compounds in maize (Zea mays L.) roots and leaves after 7 day-treatment in hydroponics. The sub-cellular compartmentalization and bio-macromolecular changes induced by 5 mu M Cu applied alone or with CDs (167 and 500 mg/L) were studied using the Synchrotron-based Fourier transformmicro-spectroscopy (SR-FTIR) combined with X-Ray photoelectron spectroscopy (XPS). Cu induced changes in content of cell wall polysaccharides, proteins, and lipids. The XPS detected CDs transport throughout the plants. The Cu/167CDs treatment reduced Cu concentration in the roots, possibly by complexation/trapping between the functional groups on CDs surface and Cu2+. Principal component analysis of FTIR spectra confirmed that Cu/500CDs treatment increased Cu adverse effects in most tissues but alleviated adverse Cu effects on cell wall polysaccharides in the root xylem, and on polysaccharides and proteins in leaf phloem and mesophyll.
PB  - Elsevier, Amsterdam
T2  - Colloids and Surfaces B-Biointerfaces
T1  - Estimation of carbon dots amelioration of copper toxicity in maize studied by synchrotron radiation-FTIR
VL  - 204
DO  - 10.1016/j.colsurfb.2021.111828
ER  - 

@article{
author = "Ducic, Tanja and Milenković, Ivana and Mutavdžić, Dragosav and Nikolic, Miroslav and Martinez, de Yuso, M. Valle and Vučinić, Željko and Algarra, Manuel and Radotić, Ksenija",
year = "2021",
abstract = "Carbon dots are biocompatible and non-toxic nanoparticles with chemical affinity to some heavy metals. Human activities increase soil pollution with copper. Cu is an essential microelement in plants, but excess can induce a harmful effects. In plant response to Cu, the cell wall plays an important role. This study aims to estimate possible amelioration effects of folic acid based CDs on Cu toxicity by studying the intracellular and cell wall compounds in maize (Zea mays L.) roots and leaves after 7 day-treatment in hydroponics. The sub-cellular compartmentalization and bio-macromolecular changes induced by 5 mu M Cu applied alone or with CDs (167 and 500 mg/L) were studied using the Synchrotron-based Fourier transformmicro-spectroscopy (SR-FTIR) combined with X-Ray photoelectron spectroscopy (XPS). Cu induced changes in content of cell wall polysaccharides, proteins, and lipids. The XPS detected CDs transport throughout the plants. The Cu/167CDs treatment reduced Cu concentration in the roots, possibly by complexation/trapping between the functional groups on CDs surface and Cu2+. Principal component analysis of FTIR spectra confirmed that Cu/500CDs treatment increased Cu adverse effects in most tissues but alleviated adverse Cu effects on cell wall polysaccharides in the root xylem, and on polysaccharides and proteins in leaf phloem and mesophyll.",
publisher = "Elsevier, Amsterdam",
journal = "Colloids and Surfaces B-Biointerfaces",
title = "Estimation of carbon dots amelioration of copper toxicity in maize studied by synchrotron radiation-FTIR",
volume = "204",
doi = "10.1016/j.colsurfb.2021.111828"
}

Ducic, T., Milenković, I., Mutavdžić, D., Nikolic, M., Martinez, d. Y. M. V., Vučinić, Ž., Algarra, M.,& Radotić, K.. (2021). Estimation of carbon dots amelioration of copper toxicity in maize studied by synchrotron radiation-FTIR. in Colloids and Surfaces B-Biointerfaces
Elsevier, Amsterdam., 204.
https://doi.org/10.1016/j.colsurfb.2021.111828

Ducic T, Milenković I, Mutavdžić D, Nikolic M, Martinez DYMV, Vučinić Ž, Algarra M, Radotić K. Estimation of carbon dots amelioration of copper toxicity in maize studied by synchrotron radiation-FTIR. in Colloids and Surfaces B-Biointerfaces. 2021;204.
doi:10.1016/j.colsurfb.2021.111828 .

Ducic, Tanja, Milenković, Ivana, Mutavdžić, Dragosav, Nikolic, Miroslav, Martinez, de Yuso, M. Valle, Vučinić, Željko, Algarra, Manuel, Radotić, Ksenija, "Estimation of carbon dots amelioration of copper toxicity in maize studied by synchrotron radiation-FTIR" in Colloids and Surfaces B-Biointerfaces, 204 (2021),
https://doi.org/10.1016/j.colsurfb.2021.111828 . .

TY  - JOUR
AU  - Vojvodić, Snežana
AU  - Stanić, Marina
AU  - Zechmann, Bernd
AU  - Ducic, Tanja
AU  - Žižić, Milan
AU  - Dimitrijević, Milena
AU  - Danilović Luković, Jelena
AU  - Milenković, Milica R.
AU  - Pittman, Jon K.
AU  - Spasojević, Ivan
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1354
AB  - Microalgae have evolved mechanisms to respond to changes in copper ion availability, which are very important for normal cellular function, to tolerate metal pollution of aquatic ecosystems, and for modulation of copper bioavailability and toxicity to other organisms. Knowledge and application of these mechanisms will benefit the use of microalgae in wastewater processing and biomass production, and the use of copper compounds in the suppression of harmful algal blooms. Here, using electron microscopy, synchrotron radiation-based Fourier transform infrared spectroscopy, electron paramagnetic resonance spectroscopy, and X-ray absorption fine structure spectroscopy, we show that the microalga Chlorella sorokiniana responds promptly to Cu2+ at high non-toxic concentration, by mucilage release, alterations in the architecture of the outer cell wall layer and lipid structures, and polyphosphate accumulation within mucilage matrix. The main route of copper detoxification is by Cu2+ coordination to polyphosphates in penta-coordinated geometry. The sequestrated Cu2+ was accessible and could be released by extracellular chelating agents. Finally, the reduction in Cu2+ to Cu1+ appears also to take place. These findings reveal the biochemical basis of the capacity of microalgae to adapt to high external copper concentrations and to serve as both, sinks and pools of environmental copper.
PB  - Portland Press Ltd, London
T2  - Biochemical Journal
T1  - Mechanisms of detoxification of high copper concentrations by the microalga Chlorella sorokiniana
EP  - 3741
IS  - 19
SP  - 3729
VL  - 477
DO  - 10.1042/BCJ20200600
ER  - 

@article{
author = "Vojvodić, Snežana and Stanić, Marina and Zechmann, Bernd and Ducic, Tanja and Žižić, Milan and Dimitrijević, Milena and Danilović Luković, Jelena and Milenković, Milica R. and Pittman, Jon K. and Spasojević, Ivan",
year = "2020",
abstract = "Microalgae have evolved mechanisms to respond to changes in copper ion availability, which are very important for normal cellular function, to tolerate metal pollution of aquatic ecosystems, and for modulation of copper bioavailability and toxicity to other organisms. Knowledge and application of these mechanisms will benefit the use of microalgae in wastewater processing and biomass production, and the use of copper compounds in the suppression of harmful algal blooms. Here, using electron microscopy, synchrotron radiation-based Fourier transform infrared spectroscopy, electron paramagnetic resonance spectroscopy, and X-ray absorption fine structure spectroscopy, we show that the microalga Chlorella sorokiniana responds promptly to Cu2+ at high non-toxic concentration, by mucilage release, alterations in the architecture of the outer cell wall layer and lipid structures, and polyphosphate accumulation within mucilage matrix. The main route of copper detoxification is by Cu2+ coordination to polyphosphates in penta-coordinated geometry. The sequestrated Cu2+ was accessible and could be released by extracellular chelating agents. Finally, the reduction in Cu2+ to Cu1+ appears also to take place. These findings reveal the biochemical basis of the capacity of microalgae to adapt to high external copper concentrations and to serve as both, sinks and pools of environmental copper.",
publisher = "Portland Press Ltd, London",
journal = "Biochemical Journal",
title = "Mechanisms of detoxification of high copper concentrations by the microalga Chlorella sorokiniana",
pages = "3741-3729",
number = "19",
volume = "477",
doi = "10.1042/BCJ20200600"
}

Vojvodić, S., Stanić, M., Zechmann, B., Ducic, T., Žižić, M., Dimitrijević, M., Danilović Luković, J., Milenković, M. R., Pittman, J. K.,& Spasojević, I.. (2020). Mechanisms of detoxification of high copper concentrations by the microalga Chlorella sorokiniana. in Biochemical Journal
Portland Press Ltd, London., 477(19), 3729-3741.
https://doi.org/10.1042/BCJ20200600

Vojvodić S, Stanić M, Zechmann B, Ducic T, Žižić M, Dimitrijević M, Danilović Luković J, Milenković MR, Pittman JK, Spasojević I. Mechanisms of detoxification of high copper concentrations by the microalga Chlorella sorokiniana. in Biochemical Journal. 2020;477(19):3729-3741.
doi:10.1042/BCJ20200600 .

Vojvodić, Snežana, Stanić, Marina, Zechmann, Bernd, Ducic, Tanja, Žižić, Milan, Dimitrijević, Milena, Danilović Luković, Jelena, Milenković, Milica R., Pittman, Jon K., Spasojević, Ivan, "Mechanisms of detoxification of high copper concentrations by the microalga Chlorella sorokiniana" in Biochemical Journal, 477, no. 19 (2020):3729-3741,
https://doi.org/10.1042/BCJ20200600 . .

TY  - JOUR
AU  - Zakrzewska, Joanna
AU  - Mitrović, Aleksandra Lj.
AU  - Mutavdžić, Dragosav
AU  - Ducic, Tanja
AU  - Radotić, Ksenija
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1394
AB  - The phosphates (Pi) are nowadays recognized as pollutants. We studied the effect of Pi (0.625-12.500 mM KH2PO4) in the culture medium on in vitro grown 2-month-old Populus alba trees. The levels of sugar phosphates and vacuolar and cytoplasmic Pi in cell compartments of roots and stems were determined using P-31 NMR, while tissue-specific micro- and macroelements mapping on stem cross-sections were performed using synchrotron-based X-ray microfluorescence. Plants grown on 0.625 mM Pi (MS/2 medium) showed a survival rate of 70%. With the increase in Pi concentrations up to 6.250 mM, plant growth and survival increased, without changes in total P content per mass or in the levels of cytoplasmic and vacuolar phosphates, in both stems and roots, while the levels of Fe, Cu, Zn, Ca and Mn in stems increased. Further increase in Pi to 9.375 and 12.500 mM in the medium resulted in inhibited growth comparable with plants grown on MS/2, with the increase in total P content per mass up to 50%, in both stems and roots, but with no changes in cytoplasmic and vacuolar phosphates; 12.500 mM Pi affected even plant survival (70%) and thus might be considered as mildly toxic. P-31 NMR results indicate that the high tolerance of P. alba to increased Pi could result from its ability to maintain an intracellular P homeostasis, despite P accumulation up to 50%, in both stems and roots, indicating P. alba as a promising wood species for dendroremediation.
PB  - Springer Heidelberg, Heidelberg
T2  - Environmental Science and Pollution Research
T1  - Phosphorus homeostasis in Populus alba L. under excess phosphate conditions, assessed by 31P nuclear magnetic resonance spectroscopy and X-ray microfluorescence
EP  - 3328
IS  - 3
SP  - 3320
VL  - 27
DO  - 10.1007/s11356-019-07200-9
ER  - 

@article{
author = "Zakrzewska, Joanna and Mitrović, Aleksandra Lj. and Mutavdžić, Dragosav and Ducic, Tanja and Radotić, Ksenija",
year = "2020",
abstract = "The phosphates (Pi) are nowadays recognized as pollutants. We studied the effect of Pi (0.625-12.500 mM KH2PO4) in the culture medium on in vitro grown 2-month-old Populus alba trees. The levels of sugar phosphates and vacuolar and cytoplasmic Pi in cell compartments of roots and stems were determined using P-31 NMR, while tissue-specific micro- and macroelements mapping on stem cross-sections were performed using synchrotron-based X-ray microfluorescence. Plants grown on 0.625 mM Pi (MS/2 medium) showed a survival rate of 70%. With the increase in Pi concentrations up to 6.250 mM, plant growth and survival increased, without changes in total P content per mass or in the levels of cytoplasmic and vacuolar phosphates, in both stems and roots, while the levels of Fe, Cu, Zn, Ca and Mn in stems increased. Further increase in Pi to 9.375 and 12.500 mM in the medium resulted in inhibited growth comparable with plants grown on MS/2, with the increase in total P content per mass up to 50%, in both stems and roots, but with no changes in cytoplasmic and vacuolar phosphates; 12.500 mM Pi affected even plant survival (70%) and thus might be considered as mildly toxic. P-31 NMR results indicate that the high tolerance of P. alba to increased Pi could result from its ability to maintain an intracellular P homeostasis, despite P accumulation up to 50%, in both stems and roots, indicating P. alba as a promising wood species for dendroremediation.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Environmental Science and Pollution Research",
title = "Phosphorus homeostasis in Populus alba L. under excess phosphate conditions, assessed by 31P nuclear magnetic resonance spectroscopy and X-ray microfluorescence",
pages = "3328-3320",
number = "3",
volume = "27",
doi = "10.1007/s11356-019-07200-9"
}

Zakrzewska, J., Mitrović, A. Lj., Mutavdžić, D., Ducic, T.,& Radotić, K.. (2020). Phosphorus homeostasis in Populus alba L. under excess phosphate conditions, assessed by 31P nuclear magnetic resonance spectroscopy and X-ray microfluorescence. in Environmental Science and Pollution Research
Springer Heidelberg, Heidelberg., 27(3), 3320-3328.
https://doi.org/10.1007/s11356-019-07200-9

Zakrzewska J, Mitrović AL, Mutavdžić D, Ducic T, Radotić K. Phosphorus homeostasis in Populus alba L. under excess phosphate conditions, assessed by 31P nuclear magnetic resonance spectroscopy and X-ray microfluorescence. in Environmental Science and Pollution Research. 2020;27(3):3320-3328.
doi:10.1007/s11356-019-07200-9 .

Zakrzewska, Joanna, Mitrović, Aleksandra Lj., Mutavdžić, Dragosav, Ducic, Tanja, Radotić, Ksenija, "Phosphorus homeostasis in Populus alba L. under excess phosphate conditions, assessed by 31P nuclear magnetic resonance spectroscopy and X-ray microfluorescence" in Environmental Science and Pollution Research, 27, no. 3 (2020):3320-3328,
https://doi.org/10.1007/s11356-019-07200-9 . .

TY  - JOUR
AU  - Žižić, Milan
AU  - Ducic, Tanja
AU  - Grolimund, Daniel
AU  - Bajuk-Bogdanović, Danica
AU  - Nikolic, Miroslav
AU  - Stanić, Marina
AU  - Krizak, Strahinja
AU  - Zakrzewska, Joanna
PY  - 2015
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/862
AB  - Vanadium speciation in the fungus Phycomyces blakesleeanus was examined by X-ray absorption near-edge structure (XANES) spectroscopy, enabling assessment of oxidation states and related molecular symmetries of this transition element in the fungus. The exposure of P. blakesleeanus to two physiologically important vanadium species (V5+ and V4+) resulted in the accumulation of this metal in central compartments of 24 h old mycelia, most probably in vacuoles. Tetrahedral V5+, octahedral V4+, and proposed intracellular complexes of V5+ were detected simultaneously after addition of a physiologically relevant concentration of V5+ to the mycelium. A substantial fraction of the externally added V4+ remained mostly in its original form. However, observable variations in the pre-edge-peak intensities in the XANES spectra indicated intracellular complexation and corresponding changes in the molecular coordination symmetry. Vanadate complexation was confirmed by V-51 NMR and Raman spectroscopy, and potential binding compounds including cell-wall constituents (chitosan and/or chitin), (poly)phosphates, DNA, and proteins are proposed. The evidenced vanadate complexation and reduction could also explain the resistance of P. blakesleeanus to high extracellular concentrations of vanadium.
PB  - Springer Heidelberg, Heidelberg
T2  - Analytical and Bioanalytical Chemistry
T1  - X-ray absorption near-edge structure micro-spectroscopy study of vanadium speciation in Phycomyces blakesleeanus mycelium
EP  - 7496
IS  - 24
SP  - 7487
VL  - 407
DO  - 10.1007/s00216-015-8916-7
ER  - 

@article{
author = "Žižić, Milan and Ducic, Tanja and Grolimund, Daniel and Bajuk-Bogdanović, Danica and Nikolic, Miroslav and Stanić, Marina and Krizak, Strahinja and Zakrzewska, Joanna",
year = "2015",
abstract = "Vanadium speciation in the fungus Phycomyces blakesleeanus was examined by X-ray absorption near-edge structure (XANES) spectroscopy, enabling assessment of oxidation states and related molecular symmetries of this transition element in the fungus. The exposure of P. blakesleeanus to two physiologically important vanadium species (V5+ and V4+) resulted in the accumulation of this metal in central compartments of 24 h old mycelia, most probably in vacuoles. Tetrahedral V5+, octahedral V4+, and proposed intracellular complexes of V5+ were detected simultaneously after addition of a physiologically relevant concentration of V5+ to the mycelium. A substantial fraction of the externally added V4+ remained mostly in its original form. However, observable variations in the pre-edge-peak intensities in the XANES spectra indicated intracellular complexation and corresponding changes in the molecular coordination symmetry. Vanadate complexation was confirmed by V-51 NMR and Raman spectroscopy, and potential binding compounds including cell-wall constituents (chitosan and/or chitin), (poly)phosphates, DNA, and proteins are proposed. The evidenced vanadate complexation and reduction could also explain the resistance of P. blakesleeanus to high extracellular concentrations of vanadium.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Analytical and Bioanalytical Chemistry",
title = "X-ray absorption near-edge structure micro-spectroscopy study of vanadium speciation in Phycomyces blakesleeanus mycelium",
pages = "7496-7487",
number = "24",
volume = "407",
doi = "10.1007/s00216-015-8916-7"
}

Žižić, M., Ducic, T., Grolimund, D., Bajuk-Bogdanović, D., Nikolic, M., Stanić, M., Krizak, S.,& Zakrzewska, J.. (2015). X-ray absorption near-edge structure micro-spectroscopy study of vanadium speciation in Phycomyces blakesleeanus mycelium. in Analytical and Bioanalytical Chemistry
Springer Heidelberg, Heidelberg., 407(24), 7487-7496.
https://doi.org/10.1007/s00216-015-8916-7

Žižić M, Ducic T, Grolimund D, Bajuk-Bogdanović D, Nikolic M, Stanić M, Krizak S, Zakrzewska J. X-ray absorption near-edge structure micro-spectroscopy study of vanadium speciation in Phycomyces blakesleeanus mycelium. in Analytical and Bioanalytical Chemistry. 2015;407(24):7487-7496.
doi:10.1007/s00216-015-8916-7 .

Žižić, Milan, Ducic, Tanja, Grolimund, Daniel, Bajuk-Bogdanović, Danica, Nikolic, Miroslav, Stanić, Marina, Krizak, Strahinja, Zakrzewska, Joanna, "X-ray absorption near-edge structure micro-spectroscopy study of vanadium speciation in Phycomyces blakesleeanus mycelium" in Analytical and Bioanalytical Chemistry, 407, no. 24 (2015):7487-7496,
https://doi.org/10.1007/s00216-015-8916-7 . .

TY  - JOUR
AU  - Ducic, Tanja
AU  - Borchert, Manuela
AU  - Savić, Aleksandar G
AU  - Kalauzi, Aleksandar
AU  - Mitrović, Aleksandra Lj.
AU  - Radotić, Ksenija
PY  - 2013
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/696
AB  - Synchrotron-based X-ray microfluorescence (mu SXRF) is an analytical method suitable for in situ investigation of the distribution of micronutrient and macronutrient elements in several-micrometres-thick unstained biological samples, e. g. single cells and tissues. Elements are mapped and quantified at sub-p. p. m. concentrations. In this study the quantity, distribution and grouping/co-localization of various elements have been identified in straight and twisted internodes of the stems of the monocotyledonous climber D. balcanica Kosanin. Three different statistical methods were employed to analyse the macronutrient and micronutrient distributions and co-localization. Macronutrient elements (K, P, Ca, Cl) are distributed homogeneously in both straight and twisted internodes. Micronutrient elements are mostly grouped in the vasculature and in the sclerenchyma cell layer. In addition, co-localization of micronutrient elements is much more prominent in twisted than in straight internodes. These image analyses and statistical methods provided very similar outcomes and could be applied to various types of biological samples imaged by mSXRF.
PB  - Wiley-Blackwell, Hoboken
T2  - Journal of Synchrotron Radiation
T1  - Enhancement in statistical and image analysis for in situ mu SXRF studies of elemental distribution and co-localization, using Dioscorea balcanica
EP  - 346
SP  - 339
VL  - 20
DO  - 10.1107/S0909049512050170
ER  - 

@article{
author = "Ducic, Tanja and Borchert, Manuela and Savić, Aleksandar G and Kalauzi, Aleksandar and Mitrović, Aleksandra Lj. and Radotić, Ksenija",
year = "2013",
abstract = "Synchrotron-based X-ray microfluorescence (mu SXRF) is an analytical method suitable for in situ investigation of the distribution of micronutrient and macronutrient elements in several-micrometres-thick unstained biological samples, e. g. single cells and tissues. Elements are mapped and quantified at sub-p. p. m. concentrations. In this study the quantity, distribution and grouping/co-localization of various elements have been identified in straight and twisted internodes of the stems of the monocotyledonous climber D. balcanica Kosanin. Three different statistical methods were employed to analyse the macronutrient and micronutrient distributions and co-localization. Macronutrient elements (K, P, Ca, Cl) are distributed homogeneously in both straight and twisted internodes. Micronutrient elements are mostly grouped in the vasculature and in the sclerenchyma cell layer. In addition, co-localization of micronutrient elements is much more prominent in twisted than in straight internodes. These image analyses and statistical methods provided very similar outcomes and could be applied to various types of biological samples imaged by mSXRF.",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Journal of Synchrotron Radiation",
title = "Enhancement in statistical and image analysis for in situ mu SXRF studies of elemental distribution and co-localization, using Dioscorea balcanica",
pages = "346-339",
volume = "20",
doi = "10.1107/S0909049512050170"
}

Ducic, T., Borchert, M., Savić, A. G., Kalauzi, A., Mitrović, A. Lj.,& Radotić, K.. (2013). Enhancement in statistical and image analysis for in situ mu SXRF studies of elemental distribution and co-localization, using Dioscorea balcanica. in Journal of Synchrotron Radiation
Wiley-Blackwell, Hoboken., 20, 339-346.
https://doi.org/10.1107/S0909049512050170

Ducic T, Borchert M, Savić AG, Kalauzi A, Mitrović AL, Radotić K. Enhancement in statistical and image analysis for in situ mu SXRF studies of elemental distribution and co-localization, using Dioscorea balcanica. in Journal of Synchrotron Radiation. 2013;20:339-346.
doi:10.1107/S0909049512050170 .

Ducic, Tanja, Borchert, Manuela, Savić, Aleksandar G, Kalauzi, Aleksandar, Mitrović, Aleksandra Lj., Radotić, Ksenija, "Enhancement in statistical and image analysis for in situ mu SXRF studies of elemental distribution and co-localization, using Dioscorea balcanica" in Journal of Synchrotron Radiation, 20 (2013):339-346,
https://doi.org/10.1107/S0909049512050170 . .

TY  - JOUR
AU  - Ducic, Tanja
AU  - Maksimović, Vuk
AU  - Radotić, Ksenija
PY  - 2008
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/242
AB  - We studied changes in the concentrations of ascorbate and glutathione, composition of soluble phenolics, and activity of oxalate oxidase in 75-day-old Serbian spruce plants after exposure to 5 mu M and 50 mu M cadmium for 6-48 h. The presence of OxOx activity in a conifer species is here demonstrated for the first time. Both Cd concentrations induced a decrease of OxOx activity in treated plants in comparison with the control at all sampling dates. The concentrations of reduced glutathione, its oxidized form, and reduced ascorbate in the plants decreased during 48-h treatment with cadmium. Among simple phenolics, only catechin increased significantly during Cd treatment.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Oxalate oxidase and non-enzymatic compounds of the antioxidative system in young Serbian spruce plants exposed to cadmium stress
EP  - 76
IS  - 1
SP  - 67
VL  - 60
DO  - 10.2298/ABS0801067D
ER  - 

@article{
author = "Ducic, Tanja and Maksimović, Vuk and Radotić, Ksenija",
year = "2008",
abstract = "We studied changes in the concentrations of ascorbate and glutathione, composition of soluble phenolics, and activity of oxalate oxidase in 75-day-old Serbian spruce plants after exposure to 5 mu M and 50 mu M cadmium for 6-48 h. The presence of OxOx activity in a conifer species is here demonstrated for the first time. Both Cd concentrations induced a decrease of OxOx activity in treated plants in comparison with the control at all sampling dates. The concentrations of reduced glutathione, its oxidized form, and reduced ascorbate in the plants decreased during 48-h treatment with cadmium. Among simple phenolics, only catechin increased significantly during Cd treatment.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Oxalate oxidase and non-enzymatic compounds of the antioxidative system in young Serbian spruce plants exposed to cadmium stress",
pages = "76-67",
number = "1",
volume = "60",
doi = "10.2298/ABS0801067D"
}

Ducic, T., Maksimović, V.,& Radotić, K.. (2008). Oxalate oxidase and non-enzymatic compounds of the antioxidative system in young Serbian spruce plants exposed to cadmium stress. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 60(1), 67-76.
https://doi.org/10.2298/ABS0801067D

Ducic T, Maksimović V, Radotić K. Oxalate oxidase and non-enzymatic compounds of the antioxidative system in young Serbian spruce plants exposed to cadmium stress. in Archives of Biological Sciences. 2008;60(1):67-76.
doi:10.2298/ABS0801067D .

Ducic, Tanja, Maksimović, Vuk, Radotić, Ksenija, "Oxalate oxidase and non-enzymatic compounds of the antioxidative system in young Serbian spruce plants exposed to cadmium stress" in Archives of Biological Sciences, 60, no. 1 (2008):67-76,
https://doi.org/10.2298/ABS0801067D . .

TY  - JOUR
AU  - Bogdanović Pristov, Jelena
AU  - Milosavić, Nenad
AU  - Prodanović, Radivoje
AU  - Ducic, Tanja
AU  - Radotić, Ksenija
PY  - 2007
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/233
AB  - Variations were studied of the activity and isoenzyme patterns of soluble peroxidase, catalase, catechol oxidase and superoxide dismutase, in needles of the Balkan endemic conifer Serbian spruce, Picea omorika (Panc.) Purkinye. The samples were collected from the natural habitat of the species, Mt. Tara. Seasonal changes were found to affect enzymatic activities and isoenzyme profiles. Total protein content was significantly lower in the summer than in other seasons. Several isoforms of peroxidase, catechol oxidase and superoxide dismutase (SOD), as well as two catalase isoenzymes were detected. The number of peroxidase isoenzymes was greatest during the vegetative season. Catalase and catechol oxidase peaked in summer and spring, respectively. Total SOD and Mn-SOD activities were significantly higher in the winter samples than the summer ones.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Biochemical Systematics and Ecology
T1  - Variability of antioxidant enzyme activity and isoenzyme profile in needles of Serbian spruce (Picea omorika (Panc.) Purkinye)
EP  - 273
IS  - 5
SP  - 263
VL  - 35
DO  - 10.1016/j.bse.2006.12.001
ER  - 

@article{
author = "Bogdanović Pristov, Jelena and Milosavić, Nenad and Prodanović, Radivoje and Ducic, Tanja and Radotić, Ksenija",
year = "2007",
abstract = "Variations were studied of the activity and isoenzyme patterns of soluble peroxidase, catalase, catechol oxidase and superoxide dismutase, in needles of the Balkan endemic conifer Serbian spruce, Picea omorika (Panc.) Purkinye. The samples were collected from the natural habitat of the species, Mt. Tara. Seasonal changes were found to affect enzymatic activities and isoenzyme profiles. Total protein content was significantly lower in the summer than in other seasons. Several isoforms of peroxidase, catechol oxidase and superoxide dismutase (SOD), as well as two catalase isoenzymes were detected. The number of peroxidase isoenzymes was greatest during the vegetative season. Catalase and catechol oxidase peaked in summer and spring, respectively. Total SOD and Mn-SOD activities were significantly higher in the winter samples than the summer ones.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Biochemical Systematics and Ecology",
title = "Variability of antioxidant enzyme activity and isoenzyme profile in needles of Serbian spruce (Picea omorika (Panc.) Purkinye)",
pages = "273-263",
number = "5",
volume = "35",
doi = "10.1016/j.bse.2006.12.001"
}

Bogdanović Pristov, J., Milosavić, N., Prodanović, R., Ducic, T.,& Radotić, K.. (2007). Variability of antioxidant enzyme activity and isoenzyme profile in needles of Serbian spruce (Picea omorika (Panc.) Purkinye). in Biochemical Systematics and Ecology
Pergamon-Elsevier Science Ltd, Oxford., 35(5), 263-273.
https://doi.org/10.1016/j.bse.2006.12.001

Bogdanović Pristov J, Milosavić N, Prodanović R, Ducic T, Radotić K. Variability of antioxidant enzyme activity and isoenzyme profile in needles of Serbian spruce (Picea omorika (Panc.) Purkinye). in Biochemical Systematics and Ecology. 2007;35(5):263-273.
doi:10.1016/j.bse.2006.12.001 .

Bogdanović Pristov, Jelena, Milosavić, Nenad, Prodanović, Radivoje, Ducic, Tanja, Radotić, Ksenija, "Variability of antioxidant enzyme activity and isoenzyme profile in needles of Serbian spruce (Picea omorika (Panc.) Purkinye)" in Biochemical Systematics and Ecology, 35, no. 5 (2007):263-273,
https://doi.org/10.1016/j.bse.2006.12.001 . .

TY  - JOUR
AU  - Mitrović, Aleksandra Lj.
AU  - Ducic, Tanja
AU  - Liric-Rajlic, I
AU  - Radotić, Ksenija
AU  - Živanović, Branka  D.
PY  - 2005
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/133
AB  - We studied antioxidative system, germination, growth, and flowering in vitro in Chenopodium rubrum seeds of different ages. Peroxidase, superoxide dismutase, and catalase activity, as well as glutathione status, were determined in 2.5-h imbibed seeds. Germination was tested under controlled conditions. Growth and flowering of plants were tested in vitro. The enzyme activities and glutathione content were higher in younger seeds. Germination declines with seed age. Plants derived from older seeds were smaller, and flowering percentage was lower compared to plants derived from younger seeds. Gibberellic acid reduced the difference in growth and flowering between plants derived from seeds different in age.
PB  - New York Acad Sciences, New York
T2  - Biophysics From Molecules to Brain: in Memory of Radoslav K. Andjus
T1  - Changes in Chenopodium rubrum seeds with aging
EP  - 508
SP  - 505
VL  - 1048
DO  - 10.1196/annals.1342.077
ER  - 

@article{
author = "Mitrović, Aleksandra Lj. and Ducic, Tanja and Liric-Rajlic, I and Radotić, Ksenija and Živanović, Branka  D.",
year = "2005",
abstract = "We studied antioxidative system, germination, growth, and flowering in vitro in Chenopodium rubrum seeds of different ages. Peroxidase, superoxide dismutase, and catalase activity, as well as glutathione status, were determined in 2.5-h imbibed seeds. Germination was tested under controlled conditions. Growth and flowering of plants were tested in vitro. The enzyme activities and glutathione content were higher in younger seeds. Germination declines with seed age. Plants derived from older seeds were smaller, and flowering percentage was lower compared to plants derived from younger seeds. Gibberellic acid reduced the difference in growth and flowering between plants derived from seeds different in age.",
publisher = "New York Acad Sciences, New York",
journal = "Biophysics From Molecules to Brain: in Memory of Radoslav K. Andjus",
title = "Changes in Chenopodium rubrum seeds with aging",
pages = "508-505",
volume = "1048",
doi = "10.1196/annals.1342.077"
}

Mitrović, A. Lj., Ducic, T., Liric-Rajlic, I., Radotić, K.,& Živanović, Branka  D.. (2005). Changes in Chenopodium rubrum seeds with aging. in Biophysics From Molecules to Brain: in Memory of Radoslav K. Andjus
New York Acad Sciences, New York., 1048, 505-508.
https://doi.org/10.1196/annals.1342.077

Mitrović AL, Ducic T, Liric-Rajlic I, Radotić K, Živanović, Branka  D.. Changes in Chenopodium rubrum seeds with aging. in Biophysics From Molecules to Brain: in Memory of Radoslav K. Andjus. 2005;1048:505-508.
doi:10.1196/annals.1342.077 .

Mitrović, Aleksandra Lj., Ducic, Tanja, Liric-Rajlic, I, Radotić, Ksenija, Živanović, Branka  D., "Changes in Chenopodium rubrum seeds with aging" in Biophysics From Molecules to Brain: in Memory of Radoslav K. Andjus, 1048 (2005):505-508,
https://doi.org/10.1196/annals.1342.077 . .

TY  - JOUR
AU  - Radotić, Ksenija
AU  - Ducic, Tanja
AU  - Mutavdžić, Dragosav
PY  - 2000
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/51
AB  - We studied the guaiacol peroxidase activity, isoenzyme pattern and metal content in the needles of 2-year-old spruce grown on soils supplemented with cadmium concentrations from 1 to 21 mg kg(-1). Following exposure to cadmium, an initial increase and subsequent decrease in the activity of the soluble fraction was observed. A parallel change of their isoenzyme pattern occurred. An increase of the cell wall-bound peroxidase activity under prolonged metal treatment was evident. The results obtained show that peroxidase activity and isoenzyme pattern could be used to evaluate the capacity of one part of the defense system in spruce seedlings to withstand metal stress.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Environmental and Experimental Botany
T1  - Changes in peroxidase activity and isoenzymes in spruce needles after exposure to different concentrations of cadmium
EP  - 113
IS  - 2
SP  - 105
VL  - 44
DO  - 10.1016/S0098-8472(00)00059-9
ER  - 

@article{
author = "Radotić, Ksenija and Ducic, Tanja and Mutavdžić, Dragosav",
year = "2000",
abstract = "We studied the guaiacol peroxidase activity, isoenzyme pattern and metal content in the needles of 2-year-old spruce grown on soils supplemented with cadmium concentrations from 1 to 21 mg kg(-1). Following exposure to cadmium, an initial increase and subsequent decrease in the activity of the soluble fraction was observed. A parallel change of their isoenzyme pattern occurred. An increase of the cell wall-bound peroxidase activity under prolonged metal treatment was evident. The results obtained show that peroxidase activity and isoenzyme pattern could be used to evaluate the capacity of one part of the defense system in spruce seedlings to withstand metal stress.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Environmental and Experimental Botany",
title = "Changes in peroxidase activity and isoenzymes in spruce needles after exposure to different concentrations of cadmium",
pages = "113-105",
number = "2",
volume = "44",
doi = "10.1016/S0098-8472(00)00059-9"
}

Radotić, K., Ducic, T.,& Mutavdžić, D.. (2000). Changes in peroxidase activity and isoenzymes in spruce needles after exposure to different concentrations of cadmium. in Environmental and Experimental Botany
Pergamon-Elsevier Science Ltd, Oxford., 44(2), 105-113.
https://doi.org/10.1016/S0098-8472(00)00059-9

Radotić K, Ducic T, Mutavdžić D. Changes in peroxidase activity and isoenzymes in spruce needles after exposure to different concentrations of cadmium. in Environmental and Experimental Botany. 2000;44(2):105-113.
doi:10.1016/S0098-8472(00)00059-9 .

Radotić, Ksenija, Ducic, Tanja, Mutavdžić, Dragosav, "Changes in peroxidase activity and isoenzymes in spruce needles after exposure to different concentrations of cadmium" in Environmental and Experimental Botany, 44, no. 2 (2000):105-113,
https://doi.org/10.1016/S0098-8472(00)00059-9 . .