TY  - JOUR
AU  - Dučić, Tanja
AU  - Alves, Carla
AU  - Vučinić, Željko
AU  - Lázaro-Martínez, Juan
AU  - Petković, Marijana
AU  - Soto, Juan
AU  - Mutavdžić, Dragosav
AU  - Martinez de Yuso Garcia, Maria del Valle
AU  - Radotić, Ksenija
AU  - Algarra, Manuel
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1558
AB  - S and N-doped carbon dots (S-CDs and N-CDs) and their cisplatin (cis-Pt) derivatives.
(S-CDs@cis-Pt and N-CDs@cis-Pt) were tested on two ovarian cancer cell lines: A2780 and A2780 cells
resistant to cis-Pt (A2780R). Several spectroscopic techniques were employed to check S-CDs@cis-Pt and
N-CDs@cis-Pt: solid- and solution-state nuclear magnetic resonance, matrix-assisted laser desorption,
ionization time-of-flight mass spectrometry, and X-ray photoelectron spectroscopy. In addition,
synchrotron-based Fourier Transformed Infrared spectro-microscopy was used to evaluate the biochemical
changes in cells after treatment with cis-Pt, S-CDs, N-CDs, or S-CDs@cis-Pt and N-CDs@cis-Pt, respectively.
Computational chemistry was applied to establish the model for the most stable bond between
S-CDs and N-CDs and cis-Pt. The results revealed the successful modification of S-CDs and N-CDs with
cis-Pt and the formation of a stable composite system that can be used for drug delivery to cancer cells and likewise to overcome acquired cis-Pt resistance. Nanoparticle treatment of A2780 and A2780R cells
led to the changes in their structure of lipids, proteins, and nucleic acids depending on the treatment. The
results showed the S-CDs@cis-Pt and N-CDs@cis-Pt might be used in the combination with cis-Pt to treat
the adenocarcinoma, thus having a potential to be further developed as drug delivery systems.
PB  - Elsevier
T2  - Journal of Colloid and Interface Science
T1  - S, N-doped carbon dots-based cisplatin delivery system in adenocarcinoma cells: Spectroscopical and computational approach
EP  - 237
IS  - 623
SP  - 226
DO  - 10.1016/j.jcis.2022.05.005
ER  - 

@article{
author = "Dučić, Tanja and Alves, Carla and Vučinić, Željko and Lázaro-Martínez, Juan and Petković, Marijana and Soto, Juan and Mutavdžić, Dragosav and Martinez de Yuso Garcia, Maria del Valle and Radotić, Ksenija and Algarra, Manuel",
year = "2022",
abstract = "S and N-doped carbon dots (S-CDs and N-CDs) and their cisplatin (cis-Pt) derivatives.
(S-CDs@cis-Pt and N-CDs@cis-Pt) were tested on two ovarian cancer cell lines: A2780 and A2780 cells
resistant to cis-Pt (A2780R). Several spectroscopic techniques were employed to check S-CDs@cis-Pt and
N-CDs@cis-Pt: solid- and solution-state nuclear magnetic resonance, matrix-assisted laser desorption,
ionization time-of-flight mass spectrometry, and X-ray photoelectron spectroscopy. In addition,
synchrotron-based Fourier Transformed Infrared spectro-microscopy was used to evaluate the biochemical
changes in cells after treatment with cis-Pt, S-CDs, N-CDs, or S-CDs@cis-Pt and N-CDs@cis-Pt, respectively.
Computational chemistry was applied to establish the model for the most stable bond between
S-CDs and N-CDs and cis-Pt. The results revealed the successful modification of S-CDs and N-CDs with
cis-Pt and the formation of a stable composite system that can be used for drug delivery to cancer cells and likewise to overcome acquired cis-Pt resistance. Nanoparticle treatment of A2780 and A2780R cells
led to the changes in their structure of lipids, proteins, and nucleic acids depending on the treatment. The
results showed the S-CDs@cis-Pt and N-CDs@cis-Pt might be used in the combination with cis-Pt to treat
the adenocarcinoma, thus having a potential to be further developed as drug delivery systems.",
publisher = "Elsevier",
journal = "Journal of Colloid and Interface Science",
title = "S, N-doped carbon dots-based cisplatin delivery system in adenocarcinoma cells: Spectroscopical and computational approach",
pages = "237-226",
number = "623",
doi = "10.1016/j.jcis.2022.05.005"
}

Dučić, T., Alves, C., Vučinić, Ž., Lázaro-Martínez, J., Petković, M., Soto, J., Mutavdžić, D., Martinez de Yuso Garcia, M. d. V., Radotić, K.,& Algarra, M.. (2022). S, N-doped carbon dots-based cisplatin delivery system in adenocarcinoma cells: Spectroscopical and computational approach. in Journal of Colloid and Interface Science
Elsevier.(623), 226-237.
https://doi.org/10.1016/j.jcis.2022.05.005

Dučić T, Alves C, Vučinić Ž, Lázaro-Martínez J, Petković M, Soto J, Mutavdžić D, Martinez de Yuso Garcia MDV, Radotić K, Algarra M. S, N-doped carbon dots-based cisplatin delivery system in adenocarcinoma cells: Spectroscopical and computational approach. in Journal of Colloid and Interface Science. 2022;(623):226-237.
doi:10.1016/j.jcis.2022.05.005 .

Dučić, Tanja, Alves, Carla, Vučinić, Željko, Lázaro-Martínez, Juan, Petković, Marijana, Soto, Juan, Mutavdžić, Dragosav, Martinez de Yuso Garcia, Maria del Valle, Radotić, Ksenija, Algarra, Manuel, "S, N-doped carbon dots-based cisplatin delivery system in adenocarcinoma cells: Spectroscopical and computational approach" in Journal of Colloid and Interface Science, no. 623 (2022):226-237,
https://doi.org/10.1016/j.jcis.2022.05.005 . .

TY  - JOUR
AU  - Ducic, Tanja
AU  - Milenković, Ivana
AU  - Mutavdžić, Dragosav
AU  - Nikolic, Miroslav
AU  - Martinez, de Yuso, M. Valle
AU  - Vučinić, Željko
AU  - Algarra, Manuel
AU  - Radotić, Ksenija
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1450
AB  - Carbon dots are biocompatible and non-toxic nanoparticles with chemical affinity to some heavy metals. Human activities increase soil pollution with copper. Cu is an essential microelement in plants, but excess can induce a harmful effects. In plant response to Cu, the cell wall plays an important role. This study aims to estimate possible amelioration effects of folic acid based CDs on Cu toxicity by studying the intracellular and cell wall compounds in maize (Zea mays L.) roots and leaves after 7 day-treatment in hydroponics. The sub-cellular compartmentalization and bio-macromolecular changes induced by 5 mu M Cu applied alone or with CDs (167 and 500 mg/L) were studied using the Synchrotron-based Fourier transformmicro-spectroscopy (SR-FTIR) combined with X-Ray photoelectron spectroscopy (XPS). Cu induced changes in content of cell wall polysaccharides, proteins, and lipids. The XPS detected CDs transport throughout the plants. The Cu/167CDs treatment reduced Cu concentration in the roots, possibly by complexation/trapping between the functional groups on CDs surface and Cu2+. Principal component analysis of FTIR spectra confirmed that Cu/500CDs treatment increased Cu adverse effects in most tissues but alleviated adverse Cu effects on cell wall polysaccharides in the root xylem, and on polysaccharides and proteins in leaf phloem and mesophyll.
PB  - Elsevier, Amsterdam
T2  - Colloids and Surfaces B-Biointerfaces
T1  - Estimation of carbon dots amelioration of copper toxicity in maize studied by synchrotron radiation-FTIR
VL  - 204
DO  - 10.1016/j.colsurfb.2021.111828
ER  - 

@article{
author = "Ducic, Tanja and Milenković, Ivana and Mutavdžić, Dragosav and Nikolic, Miroslav and Martinez, de Yuso, M. Valle and Vučinić, Željko and Algarra, Manuel and Radotić, Ksenija",
year = "2021",
abstract = "Carbon dots are biocompatible and non-toxic nanoparticles with chemical affinity to some heavy metals. Human activities increase soil pollution with copper. Cu is an essential microelement in plants, but excess can induce a harmful effects. In plant response to Cu, the cell wall plays an important role. This study aims to estimate possible amelioration effects of folic acid based CDs on Cu toxicity by studying the intracellular and cell wall compounds in maize (Zea mays L.) roots and leaves after 7 day-treatment in hydroponics. The sub-cellular compartmentalization and bio-macromolecular changes induced by 5 mu M Cu applied alone or with CDs (167 and 500 mg/L) were studied using the Synchrotron-based Fourier transformmicro-spectroscopy (SR-FTIR) combined with X-Ray photoelectron spectroscopy (XPS). Cu induced changes in content of cell wall polysaccharides, proteins, and lipids. The XPS detected CDs transport throughout the plants. The Cu/167CDs treatment reduced Cu concentration in the roots, possibly by complexation/trapping between the functional groups on CDs surface and Cu2+. Principal component analysis of FTIR spectra confirmed that Cu/500CDs treatment increased Cu adverse effects in most tissues but alleviated adverse Cu effects on cell wall polysaccharides in the root xylem, and on polysaccharides and proteins in leaf phloem and mesophyll.",
publisher = "Elsevier, Amsterdam",
journal = "Colloids and Surfaces B-Biointerfaces",
title = "Estimation of carbon dots amelioration of copper toxicity in maize studied by synchrotron radiation-FTIR",
volume = "204",
doi = "10.1016/j.colsurfb.2021.111828"
}

Ducic, T., Milenković, I., Mutavdžić, D., Nikolic, M., Martinez, d. Y. M. V., Vučinić, Ž., Algarra, M.,& Radotić, K.. (2021). Estimation of carbon dots amelioration of copper toxicity in maize studied by synchrotron radiation-FTIR. in Colloids and Surfaces B-Biointerfaces
Elsevier, Amsterdam., 204.
https://doi.org/10.1016/j.colsurfb.2021.111828

Ducic T, Milenković I, Mutavdžić D, Nikolic M, Martinez DYMV, Vučinić Ž, Algarra M, Radotić K. Estimation of carbon dots amelioration of copper toxicity in maize studied by synchrotron radiation-FTIR. in Colloids and Surfaces B-Biointerfaces. 2021;204.
doi:10.1016/j.colsurfb.2021.111828 .

Ducic, Tanja, Milenković, Ivana, Mutavdžić, Dragosav, Nikolic, Miroslav, Martinez, de Yuso, M. Valle, Vučinić, Željko, Algarra, Manuel, Radotić, Ksenija, "Estimation of carbon dots amelioration of copper toxicity in maize studied by synchrotron radiation-FTIR" in Colloids and Surfaces B-Biointerfaces, 204 (2021),
https://doi.org/10.1016/j.colsurfb.2021.111828 . .

TY  - JOUR
AU  - Dragišić Maksimović, Jelena
AU  - Mojović, Miloš
AU  - Vučinić, Željko
AU  - Maksimović, Vuk
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1424
AB  - Apoplastic antioxidative constituents (enzymes, primary and secondary metabolites, ROS) from different root zones of hydroponically grown maize (Zea mays L.) were investigated using a noninvasive isolation procedure: filter strip method. Filter strips were placed at specific positions on the root surface: apical zone (tip) and basal zone (base) to absorb apoplastic fluid. Three major classes of low-weight metabolites (organic acids, sugars, and phenolics) have been identified by HPLC-ECD. The longitudinal distribution of sugars and organic acids had the same pattern: higher concentration in the tip than the base, while it was vice versa for phenolics. The specific activities of guaiacol peroxidase, superoxide dismutase, and ascorbate peroxidase were higher in the apoplastic fluid from the root base than the tip, and their different isoforms were separated by isoelectric focusing. Electron paramagnetic resonance (EPR) spectroscopy coupled with the spin-trapping method using DEPMPO showed a persistent generation of hydroxyl radical in the root tip. In vivo EPR imaging of the whole maize root with membrane-permeable and impermeable aminoxyl spin-probes, enabling real-time detection of ROS formation within and outside the membranes, demonstrated ROS accumulation on the root surface, while endodermis and central cylinder were ROS free. For the first time in plant research, 2D EPR images enabled the direct demonstration of site-specific free radical production along the root. Highly sensitive analytical techniques combined with the filter strips, as a non-invasive tool, have increased our knowledge of metabolic processes occurring in the apoplast and their spatial-temporal changes in small regions of the intact root tissue.
PB  - Wiley, Hoboken
T2  - Physiologia Plantarum
T1  - Spatial distribution of apoplastic antioxidative constituents in maize root
EP  - 828
IS  - 3
SP  - 818
VL  - 173
DO  - 10.1111/ppl.13476
ER  - 

@article{
author = "Dragišić Maksimović, Jelena and Mojović, Miloš and Vučinić, Željko and Maksimović, Vuk",
year = "2021",
abstract = "Apoplastic antioxidative constituents (enzymes, primary and secondary metabolites, ROS) from different root zones of hydroponically grown maize (Zea mays L.) were investigated using a noninvasive isolation procedure: filter strip method. Filter strips were placed at specific positions on the root surface: apical zone (tip) and basal zone (base) to absorb apoplastic fluid. Three major classes of low-weight metabolites (organic acids, sugars, and phenolics) have been identified by HPLC-ECD. The longitudinal distribution of sugars and organic acids had the same pattern: higher concentration in the tip than the base, while it was vice versa for phenolics. The specific activities of guaiacol peroxidase, superoxide dismutase, and ascorbate peroxidase were higher in the apoplastic fluid from the root base than the tip, and their different isoforms were separated by isoelectric focusing. Electron paramagnetic resonance (EPR) spectroscopy coupled with the spin-trapping method using DEPMPO showed a persistent generation of hydroxyl radical in the root tip. In vivo EPR imaging of the whole maize root with membrane-permeable and impermeable aminoxyl spin-probes, enabling real-time detection of ROS formation within and outside the membranes, demonstrated ROS accumulation on the root surface, while endodermis and central cylinder were ROS free. For the first time in plant research, 2D EPR images enabled the direct demonstration of site-specific free radical production along the root. Highly sensitive analytical techniques combined with the filter strips, as a non-invasive tool, have increased our knowledge of metabolic processes occurring in the apoplast and their spatial-temporal changes in small regions of the intact root tissue.",
publisher = "Wiley, Hoboken",
journal = "Physiologia Plantarum",
title = "Spatial distribution of apoplastic antioxidative constituents in maize root",
pages = "828-818",
number = "3",
volume = "173",
doi = "10.1111/ppl.13476"
}

Dragišić Maksimović, J., Mojović, M., Vučinić, Ž.,& Maksimović, V.. (2021). Spatial distribution of apoplastic antioxidative constituents in maize root. in Physiologia Plantarum
Wiley, Hoboken., 173(3), 818-828.
https://doi.org/10.1111/ppl.13476

Dragišić Maksimović J, Mojović M, Vučinić Ž, Maksimović V. Spatial distribution of apoplastic antioxidative constituents in maize root. in Physiologia Plantarum. 2021;173(3):818-828.
doi:10.1111/ppl.13476 .

Dragišić Maksimović, Jelena, Mojović, Miloš, Vučinić, Željko, Maksimović, Vuk, "Spatial distribution of apoplastic antioxidative constituents in maize root" in Physiologia Plantarum, 173, no. 3 (2021):818-828,
https://doi.org/10.1111/ppl.13476 . .

TY  - JOUR
AU  - Vuletic, Mirjana M
AU  - Hadzi-Tasković Sukalović, Vesna
AU  - Marković, Ksenija
AU  - Kravić, Natalija B
AU  - Vučinić, Željko
AU  - Maksimović, Vuk
PY  - 2014
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/810
AB  - An analysis of peroxidase and ascorbate oxidase activity, phenolic content and antioxidant capacity of isolated maize root cell walls was performed in controls and plants stressed with polyethylene glycol (PEG) or heavy metals, zinc or copper. Peroxidase activity (oxidative and peroxidative) was more pronounced in the ionic than in the covalent cell wall fraction. PEG induced an increase and Zn2+ a decrease of both ionically bound peroxidase activities. In the covalent fraction, Cu2+ decreased oxidative and increased peroxidative activity of peroxidase. Isoelectric focusing of ionically bound proteins and activity staining for peroxidase demonstrated increased intensities and appearance of new acidic isoforms, especially in Zn2+ and PEG treatments. Most pronounced basic isoforms (pI similar to 7.5) in controls, decreased in intensity or completely disappeared in stressed plants. Ascorbate oxidase activity was significantly increased by PEG and decreased by Zn2+ treatments, and highly correlated with peroxidase activity. Antioxidant capacity and total phenolics content increased in heavy metal-treated and decreased in PEG-treated plants. Analysis of individual phenolic components revealed p-coumaric and ferulic acids, as the most abundant, as well as ferulic acid dimers, trimers and tetramers in the cell walls; their quantity increased under stress conditions. Results presented demonstrate the existence of diverse mechanisms of plant response to different stresses.
PB  - Wiley, Hoboken
T2  - Plant Biology
T1  - Differential response of antioxidative systems of maize (Zea mays L.) roots cell walls to osmotic and heavy metal stress
EP  - 96
IS  - 1
SP  - 88
VL  - 16
DO  - 10.1111/plb.12017
ER  - 

@article{
author = "Vuletic, Mirjana M and Hadzi-Tasković Sukalović, Vesna and Marković, Ksenija and Kravić, Natalija B and Vučinić, Željko and Maksimović, Vuk",
year = "2014",
abstract = "An analysis of peroxidase and ascorbate oxidase activity, phenolic content and antioxidant capacity of isolated maize root cell walls was performed in controls and plants stressed with polyethylene glycol (PEG) or heavy metals, zinc or copper. Peroxidase activity (oxidative and peroxidative) was more pronounced in the ionic than in the covalent cell wall fraction. PEG induced an increase and Zn2+ a decrease of both ionically bound peroxidase activities. In the covalent fraction, Cu2+ decreased oxidative and increased peroxidative activity of peroxidase. Isoelectric focusing of ionically bound proteins and activity staining for peroxidase demonstrated increased intensities and appearance of new acidic isoforms, especially in Zn2+ and PEG treatments. Most pronounced basic isoforms (pI similar to 7.5) in controls, decreased in intensity or completely disappeared in stressed plants. Ascorbate oxidase activity was significantly increased by PEG and decreased by Zn2+ treatments, and highly correlated with peroxidase activity. Antioxidant capacity and total phenolics content increased in heavy metal-treated and decreased in PEG-treated plants. Analysis of individual phenolic components revealed p-coumaric and ferulic acids, as the most abundant, as well as ferulic acid dimers, trimers and tetramers in the cell walls; their quantity increased under stress conditions. Results presented demonstrate the existence of diverse mechanisms of plant response to different stresses.",
publisher = "Wiley, Hoboken",
journal = "Plant Biology",
title = "Differential response of antioxidative systems of maize (Zea mays L.) roots cell walls to osmotic and heavy metal stress",
pages = "96-88",
number = "1",
volume = "16",
doi = "10.1111/plb.12017"
}

Vuletic, M. M., Hadzi-Tasković Sukalović, V., Marković, K., Kravić, N. B., Vučinić, Ž.,& Maksimović, V.. (2014). Differential response of antioxidative systems of maize (Zea mays L.) roots cell walls to osmotic and heavy metal stress. in Plant Biology
Wiley, Hoboken., 16(1), 88-96.
https://doi.org/10.1111/plb.12017

Vuletic MM, Hadzi-Tasković Sukalović V, Marković K, Kravić NB, Vučinić Ž, Maksimović V. Differential response of antioxidative systems of maize (Zea mays L.) roots cell walls to osmotic and heavy metal stress. in Plant Biology. 2014;16(1):88-96.
doi:10.1111/plb.12017 .

Vuletic, Mirjana M, Hadzi-Tasković Sukalović, Vesna, Marković, Ksenija, Kravić, Natalija B, Vučinić, Željko, Maksimović, Vuk, "Differential response of antioxidative systems of maize (Zea mays L.) roots cell walls to osmotic and heavy metal stress" in Plant Biology, 16, no. 1 (2014):88-96,
https://doi.org/10.1111/plb.12017 . .

TY  - JOUR
AU  - Dragišić Maksimović, Jelena
AU  - Živanović, Branka  D.
AU  - Maksimović, Vuk
AU  - Mojović, Miloš
AU  - Nikolic, Miroslav
AU  - Vučinić, Željko
PY  - 2014
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/751
AB  - Apoplastic fluid was extracted from maize (Zea mays L) roots using two procedures: collection from the surface of intact plant roots by filter paper strips (AF) or vacuum infiltration and/or centrifugation from excised root segments (AWF). The content of cytoplasmic marker (glucose-6-phosphate, G-6-P) and antioxidative components (enzymes, organic acids, phenolics, sugars, ROS) were compared in the extracts. The results obtained demonstrate that AF was completely free of G-6-P, as opposed to AWF where the cytoplasmic constituent was detected even at mildest centrifugation (200 x g). Isoelectric focusing of POD and SOD shows the presence of cytoplasmic isoforms in AWF, and HPLC of sugars and phenolics a much more complex composition of AWF, due to cytoplasmic contamination. Organic acid composition differed in the two extracts, much higher concentrations of malic acid being registered in AF, while oxalic acid due to intracellular contamination being present only in AWF. EPR spectroscopy of DEPMPO spin trap in the extracts showed persistent generation of hydroxyl radical adduct in AF. The results obtained argue in favor of the filter strip method for the root apoplastic fluid extraction, avoiding the problems of cytoplasmic contamination and dilution and enabling concentration measurements in minute regions of the root.
PB  - Elsevier Ireland Ltd, Clare
T2  - Plant Science
T1  - Filter strip as a method of choice for apoplastic fluid extraction from maize roots
EP  - 58
SP  - 49
VL  - 223
DO  - 10.1016/j.plantsci.2014.03.009
ER  - 

@article{
author = "Dragišić Maksimović, Jelena and Živanović, Branka  D. and Maksimović, Vuk and Mojović, Miloš and Nikolic, Miroslav and Vučinić, Željko",
year = "2014",
abstract = "Apoplastic fluid was extracted from maize (Zea mays L) roots using two procedures: collection from the surface of intact plant roots by filter paper strips (AF) or vacuum infiltration and/or centrifugation from excised root segments (AWF). The content of cytoplasmic marker (glucose-6-phosphate, G-6-P) and antioxidative components (enzymes, organic acids, phenolics, sugars, ROS) were compared in the extracts. The results obtained demonstrate that AF was completely free of G-6-P, as opposed to AWF where the cytoplasmic constituent was detected even at mildest centrifugation (200 x g). Isoelectric focusing of POD and SOD shows the presence of cytoplasmic isoforms in AWF, and HPLC of sugars and phenolics a much more complex composition of AWF, due to cytoplasmic contamination. Organic acid composition differed in the two extracts, much higher concentrations of malic acid being registered in AF, while oxalic acid due to intracellular contamination being present only in AWF. EPR spectroscopy of DEPMPO spin trap in the extracts showed persistent generation of hydroxyl radical adduct in AF. The results obtained argue in favor of the filter strip method for the root apoplastic fluid extraction, avoiding the problems of cytoplasmic contamination and dilution and enabling concentration measurements in minute regions of the root.",
publisher = "Elsevier Ireland Ltd, Clare",
journal = "Plant Science",
title = "Filter strip as a method of choice for apoplastic fluid extraction from maize roots",
pages = "58-49",
volume = "223",
doi = "10.1016/j.plantsci.2014.03.009"
}

Dragišić Maksimović, J., Živanović, Branka  D., Maksimović, V., Mojović, M., Nikolic, M.,& Vučinić, Ž.. (2014). Filter strip as a method of choice for apoplastic fluid extraction from maize roots. in Plant Science
Elsevier Ireland Ltd, Clare., 223, 49-58.
https://doi.org/10.1016/j.plantsci.2014.03.009

Dragišić Maksimović J, Živanović, Branka  D., Maksimović V, Mojović M, Nikolic M, Vučinić Ž. Filter strip as a method of choice for apoplastic fluid extraction from maize roots. in Plant Science. 2014;223:49-58.
doi:10.1016/j.plantsci.2014.03.009 .

Dragišić Maksimović, Jelena, Živanović, Branka  D., Maksimović, Vuk, Mojović, Miloš, Nikolic, Miroslav, Vučinić, Željko, "Filter strip as a method of choice for apoplastic fluid extraction from maize roots" in Plant Science, 223 (2014):49-58,
https://doi.org/10.1016/j.plantsci.2014.03.009 . .

TY  - JOUR
AU  - Stanić, Marina
AU  - Zakrzewska, Joanna
AU  - Hadzibrahimović, Mirzeta R
AU  - Žižić, Milan
AU  - Marković, Zoran S
AU  - Vučinić, Željko
AU  - Zivić, Miroslav
PY  - 2013
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/658
AB  - Environmental changes can often result in oxygen deficiency which influences cellular energy metabolism, but such effects have been insufficiently studied in fungi. The effects of oxygen deprivation on respiration and phosphate metabolites in Phycomyces blakesleeanus were investigated by oxygen electrode and P-31 NMR spectroscopy. Mycelium was incubated in hypoxic and anoxic conditions for 1.5, 3 and 5 h and then reoxygenated. Participation of alternative oxidase (AOX) in total respiration increased gradually in both treatments and after 5 h of anoxia exceeded a value 50% higher than in control. Shortly after reintroduction of oxygen into the system AOX level decreased close to the control level. Oxygen deprivation also caused a reversible decrease of polyphosphate/inorganic phosphate ratio (PPc/Pi), which was strongly correlated with the increase of AOX participation in total respiration. Unexpectedly, ATP content remained almost constant, probably due to the ability of PolyP to sustain energy and phosphate homeostasis of the cell under stress conditions. This was further substantiated by the effects of azide, a cytochrome c oxidase inhibitor, which also decreased PPc/Pi ratio, but to a smaller extent in oxygen deprived than control and reoxygenated specimens.
PB  - Elsevier, Amsterdam
T2  - Research in Microbiology
T1  - Oxygen regulation of alternative respiration in fungus Phycomyces blakesleeanus: connection with phosphate metabolism
EP  - 778
IS  - 7
SP  - 770
VL  - 164
DO  - 10.1016/j.resmic.2013.03.002
ER  - 

@article{
author = "Stanić, Marina and Zakrzewska, Joanna and Hadzibrahimović, Mirzeta R and Žižić, Milan and Marković, Zoran S and Vučinić, Željko and Zivić, Miroslav",
year = "2013",
abstract = "Environmental changes can often result in oxygen deficiency which influences cellular energy metabolism, but such effects have been insufficiently studied in fungi. The effects of oxygen deprivation on respiration and phosphate metabolites in Phycomyces blakesleeanus were investigated by oxygen electrode and P-31 NMR spectroscopy. Mycelium was incubated in hypoxic and anoxic conditions for 1.5, 3 and 5 h and then reoxygenated. Participation of alternative oxidase (AOX) in total respiration increased gradually in both treatments and after 5 h of anoxia exceeded a value 50% higher than in control. Shortly after reintroduction of oxygen into the system AOX level decreased close to the control level. Oxygen deprivation also caused a reversible decrease of polyphosphate/inorganic phosphate ratio (PPc/Pi), which was strongly correlated with the increase of AOX participation in total respiration. Unexpectedly, ATP content remained almost constant, probably due to the ability of PolyP to sustain energy and phosphate homeostasis of the cell under stress conditions. This was further substantiated by the effects of azide, a cytochrome c oxidase inhibitor, which also decreased PPc/Pi ratio, but to a smaller extent in oxygen deprived than control and reoxygenated specimens.",
publisher = "Elsevier, Amsterdam",
journal = "Research in Microbiology",
title = "Oxygen regulation of alternative respiration in fungus Phycomyces blakesleeanus: connection with phosphate metabolism",
pages = "778-770",
number = "7",
volume = "164",
doi = "10.1016/j.resmic.2013.03.002"
}

Stanić, M., Zakrzewska, J., Hadzibrahimović, M. R., Žižić, M., Marković, Z. S., Vučinić, Ž.,& Zivić, M.. (2013). Oxygen regulation of alternative respiration in fungus Phycomyces blakesleeanus: connection with phosphate metabolism. in Research in Microbiology
Elsevier, Amsterdam., 164(7), 770-778.
https://doi.org/10.1016/j.resmic.2013.03.002

Stanić M, Zakrzewska J, Hadzibrahimović MR, Žižić M, Marković ZS, Vučinić Ž, Zivić M. Oxygen regulation of alternative respiration in fungus Phycomyces blakesleeanus: connection with phosphate metabolism. in Research in Microbiology. 2013;164(7):770-778.
doi:10.1016/j.resmic.2013.03.002 .

Stanić, Marina, Zakrzewska, Joanna, Hadzibrahimović, Mirzeta R, Žižić, Milan, Marković, Zoran S, Vučinić, Željko, Zivić, Miroslav, "Oxygen regulation of alternative respiration in fungus Phycomyces blakesleeanus: connection with phosphate metabolism" in Research in Microbiology, 164, no. 7 (2013):770-778,
https://doi.org/10.1016/j.resmic.2013.03.002 . .

TY  - JOUR
AU  - Gburcik, Verica
AU  - Mastilović, Sreten
AU  - Vučinić, Željko
PY  - 2013
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/708
AB  - Serbia aims to improve the composition of its energy sources and increase the contribution of renewable and sustainable energies to meet its growing electricity production demand. Results of many studies and numerous measurement campaigns suggest that Serbia has a considerable potential for the solar and wind energy utilization for electricity generation. The objective of this article is to present a brief assessment of these solar and wind resources. Meteorological data from a network of meteorological stations covering a period of 30 yr are used to obtain annual energy density distributions from these two climatic sources. These results, summarizing in an abbreviated form comprehensive research efforts culminating in the last decade, are presented in an archival publication for the first time. This assessment indicates that southern and southeastern Serbia are very good regions for the solar energy utilization. Southern Banat, mountain ranges of eastern and southeastern Serbia, and the lower Danube river valley are the most promising regions for the wind power plant constructions. Final objective of this article is to show that the problem of individual intermittency of wind and solar power potentials could be compensated in Serbia by their complementary character.
PB  - Amer Inst Physics, Melville
T2  - Journal of Renewable and Sustainable Energy
T1  - Assessment of solar and wind energy resources in Serbia
IS  - 4
VL  - 5
DO  - 10.1063/1.4819504
ER  - 

@article{
author = "Gburcik, Verica and Mastilović, Sreten and Vučinić, Željko",
year = "2013",
abstract = "Serbia aims to improve the composition of its energy sources and increase the contribution of renewable and sustainable energies to meet its growing electricity production demand. Results of many studies and numerous measurement campaigns suggest that Serbia has a considerable potential for the solar and wind energy utilization for electricity generation. The objective of this article is to present a brief assessment of these solar and wind resources. Meteorological data from a network of meteorological stations covering a period of 30 yr are used to obtain annual energy density distributions from these two climatic sources. These results, summarizing in an abbreviated form comprehensive research efforts culminating in the last decade, are presented in an archival publication for the first time. This assessment indicates that southern and southeastern Serbia are very good regions for the solar energy utilization. Southern Banat, mountain ranges of eastern and southeastern Serbia, and the lower Danube river valley are the most promising regions for the wind power plant constructions. Final objective of this article is to show that the problem of individual intermittency of wind and solar power potentials could be compensated in Serbia by their complementary character.",
publisher = "Amer Inst Physics, Melville",
journal = "Journal of Renewable and Sustainable Energy",
title = "Assessment of solar and wind energy resources in Serbia",
number = "4",
volume = "5",
doi = "10.1063/1.4819504"
}

Gburcik, V., Mastilović, S.,& Vučinić, Ž.. (2013). Assessment of solar and wind energy resources in Serbia. in Journal of Renewable and Sustainable Energy
Amer Inst Physics, Melville., 5(4).
https://doi.org/10.1063/1.4819504

Gburcik V, Mastilović S, Vučinić Ž. Assessment of solar and wind energy resources in Serbia. in Journal of Renewable and Sustainable Energy. 2013;5(4).
doi:10.1063/1.4819504 .

Gburcik, Verica, Mastilović, Sreten, Vučinić, Željko, "Assessment of solar and wind energy resources in Serbia" in Journal of Renewable and Sustainable Energy, 5, no. 4 (2013),
https://doi.org/10.1063/1.4819504 . .

TY  - JOUR
AU  - Hadzi-Tasković Sukalović, Vesna
AU  - Vuletic, Mirjana M
AU  - Veljović-Jovanović, Sonja
AU  - Vučinić, Željko
PY  - 2010
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/373
AB  - Here we present the results of in vitro and in vivo studies of the influence of Mn2+ and Cu2+ on the peroxidative and oxidative catalytic functions of class III peroxidase. Complex peroxidase catalysis by intermediates generated in the reaction was analyzed by utilizing the activating effect of Mn2+ and the inhibitory effect of Cu2+ on the oxidative reaction in vitro. p-Coumaric acid was used as an enzyme substrate in the peroxidative reaction and as a cofactor in the oxidative reaction. In order to correlate the observed in vitro effects with the in vivo situation, we exposed maize plants to excess concentrations of Mn2+ and Cu2+ in the hydroponic solutions. Copper severely arrested plant growth, while manganese exerted no significant effect. The effects on peroxidase activity and isoforms profile of root soluble and cell wall bound fractions were studied. Inhibition of the peroxidase oxidative function by copper was reversible, localized in the cell wall, and accompanied by disappearance of some and appearance of new cationic isoforms. Copper-mediated changes were suppressed by the presence of manganese, although Mn2+ treatment per se did not affect the activity of the peroxidase enzyme. The results on the peroxidase activity in maize roots grown with excess Mn2+ and Cu2+ point to the coupling between the oxidative cycle, root growth and different peroxidase isoforms.
PB  - Elsevier Gmbh, Munich
T2  - Journal of Plant Physiology
T1  - The effects of manganese and copper in vitro and in vivo on peroxidase catalytic cycles
EP  - 1557
IS  - 18
SP  - 1550
VL  - 167
DO  - 10.1016/j.jplph.2010.05.026
ER  - 

@article{
author = "Hadzi-Tasković Sukalović, Vesna and Vuletic, Mirjana M and Veljović-Jovanović, Sonja and Vučinić, Željko",
year = "2010",
abstract = "Here we present the results of in vitro and in vivo studies of the influence of Mn2+ and Cu2+ on the peroxidative and oxidative catalytic functions of class III peroxidase. Complex peroxidase catalysis by intermediates generated in the reaction was analyzed by utilizing the activating effect of Mn2+ and the inhibitory effect of Cu2+ on the oxidative reaction in vitro. p-Coumaric acid was used as an enzyme substrate in the peroxidative reaction and as a cofactor in the oxidative reaction. In order to correlate the observed in vitro effects with the in vivo situation, we exposed maize plants to excess concentrations of Mn2+ and Cu2+ in the hydroponic solutions. Copper severely arrested plant growth, while manganese exerted no significant effect. The effects on peroxidase activity and isoforms profile of root soluble and cell wall bound fractions were studied. Inhibition of the peroxidase oxidative function by copper was reversible, localized in the cell wall, and accompanied by disappearance of some and appearance of new cationic isoforms. Copper-mediated changes were suppressed by the presence of manganese, although Mn2+ treatment per se did not affect the activity of the peroxidase enzyme. The results on the peroxidase activity in maize roots grown with excess Mn2+ and Cu2+ point to the coupling between the oxidative cycle, root growth and different peroxidase isoforms.",
publisher = "Elsevier Gmbh, Munich",
journal = "Journal of Plant Physiology",
title = "The effects of manganese and copper in vitro and in vivo on peroxidase catalytic cycles",
pages = "1557-1550",
number = "18",
volume = "167",
doi = "10.1016/j.jplph.2010.05.026"
}

Hadzi-Tasković Sukalović, V., Vuletic, M. M., Veljović-Jovanović, S.,& Vučinić, Ž.. (2010). The effects of manganese and copper in vitro and in vivo on peroxidase catalytic cycles. in Journal of Plant Physiology
Elsevier Gmbh, Munich., 167(18), 1550-1557.
https://doi.org/10.1016/j.jplph.2010.05.026

Hadzi-Tasković Sukalović V, Vuletic MM, Veljović-Jovanović S, Vučinić Ž. The effects of manganese and copper in vitro and in vivo on peroxidase catalytic cycles. in Journal of Plant Physiology. 2010;167(18):1550-1557.
doi:10.1016/j.jplph.2010.05.026 .

Hadzi-Tasković Sukalović, Vesna, Vuletic, Mirjana M, Veljović-Jovanović, Sonja, Vučinić, Željko, "The effects of manganese and copper in vitro and in vivo on peroxidase catalytic cycles" in Journal of Plant Physiology, 167, no. 18 (2010):1550-1557,
https://doi.org/10.1016/j.jplph.2010.05.026 . .

TY  - JOUR
AU  - Kukavica, Biljana
AU  - Mojović, Miloš
AU  - Vučinić, Željko
AU  - Maksimović, Vuk
AU  - Takahama, Umeo
AU  - Veljović-Jovanović, Sonja
PY  - 2009
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/368
AB  - The hydroxyl radical produced in the apoplast has been demonstrated to facilitate cell wall loosening during cell elongation. Cell wall-bound peroxidases (PODs) have been implicated in hydroxyl radical formation. For this mechanism, the apoplast or cell walls should contain the electron donors for (i) H2O2 formation from dioxygen; and (ii) the POD-catalyzed reduction of H2O2 to the hydroxyl radical. The aim of the work was to identify the electron donors in these reactions. In this report, hydroxyl radical (OH) generation in the cell wall isolated from pea roots was detected in the absence of any exogenous reductants, suggesting that the plant cell wall possesses the capacity to generate OH in situ. Distinct POD and Mn-superoxide dismutase (Mn-SOD) isoforms different from other cellular isoforms were shown by native gel electrophoresis to be preferably bound to the cell walls. Electron paramagnetic resonance (EPR) spectroscopy of cell wall isolates containing the spin-trapping reagent, 5-diethoxyphosphoryl-5-methyl-1-pyrroline-N-oxide (DEPMPO), was used for detection of and differentiation between OH and the superoxide radical (O-2). The data obtained using POD inhibitors confirmed that tightly bound cell wall PODs are involved in DEPMPOOH adduct formation. A decrease in DEPMPOOH adduct formation in the presence of H2O2 scavengers demonstrated that this hydroxyl radical was derived from H2O2. During the generation of OH, the concentration of quinhydrone structures (as detected by EPR spectroscopy) increased, suggesting that the H2O2 required for the formation of OH in isolated cell walls is produced during the reduction of O-2 by hydroxycinnamic acids. Cell wall isolates in which the proteins have been denaturated (including the endogenous POD and SOD) did not produce OH. Addition of exogenous H2O2 again induced the production of OH, and these were shown to originate from the Fenton reaction with tightly bound metal ions. However, the appearance of the DEPMPOOOH adduct could also be observed, due to the production of O-2 when endogenous SOD has been inactivated. Also, O-2 was converted to OH in an in vitro horseradish peroxidase (HRP)H2O2 system to which exogenous SOD has been added. Taken together with the discovery of the cell wall-bound Mn-SOD isoform, these results support the role of such a cell wall-bound SOD in the formation of OH jointly with the cell wall-bound POD. According to the above findings, it seems that the hydroxycinnamic acids from the cell wall, acting as reductants, contribute to the formation of H2O2 in the presence of O-2 in an autocatalytic manner, and that POD and Mn-SOD coupled together generate OH from such H2O2.
PB  - Oxford Univ Press, Oxford
T2  - Plant and Cell Physiology
T1  - Generation of Hydroxyl Radical in Isolated Pea Root Cell Wall, and the Role of Cell Wall-Bound Peroxidase, Mn-SOD and Phenolics in Their Production
EP  - 317
IS  - 2
SP  - 304
VL  - 50
DO  - 10.1093/pcp/pcn199
ER  - 

@article{
author = "Kukavica, Biljana and Mojović, Miloš and Vučinić, Željko and Maksimović, Vuk and Takahama, Umeo and Veljović-Jovanović, Sonja",
year = "2009",
abstract = "The hydroxyl radical produced in the apoplast has been demonstrated to facilitate cell wall loosening during cell elongation. Cell wall-bound peroxidases (PODs) have been implicated in hydroxyl radical formation. For this mechanism, the apoplast or cell walls should contain the electron donors for (i) H2O2 formation from dioxygen; and (ii) the POD-catalyzed reduction of H2O2 to the hydroxyl radical. The aim of the work was to identify the electron donors in these reactions. In this report, hydroxyl radical (OH) generation in the cell wall isolated from pea roots was detected in the absence of any exogenous reductants, suggesting that the plant cell wall possesses the capacity to generate OH in situ. Distinct POD and Mn-superoxide dismutase (Mn-SOD) isoforms different from other cellular isoforms were shown by native gel electrophoresis to be preferably bound to the cell walls. Electron paramagnetic resonance (EPR) spectroscopy of cell wall isolates containing the spin-trapping reagent, 5-diethoxyphosphoryl-5-methyl-1-pyrroline-N-oxide (DEPMPO), was used for detection of and differentiation between OH and the superoxide radical (O-2). The data obtained using POD inhibitors confirmed that tightly bound cell wall PODs are involved in DEPMPOOH adduct formation. A decrease in DEPMPOOH adduct formation in the presence of H2O2 scavengers demonstrated that this hydroxyl radical was derived from H2O2. During the generation of OH, the concentration of quinhydrone structures (as detected by EPR spectroscopy) increased, suggesting that the H2O2 required for the formation of OH in isolated cell walls is produced during the reduction of O-2 by hydroxycinnamic acids. Cell wall isolates in which the proteins have been denaturated (including the endogenous POD and SOD) did not produce OH. Addition of exogenous H2O2 again induced the production of OH, and these were shown to originate from the Fenton reaction with tightly bound metal ions. However, the appearance of the DEPMPOOOH adduct could also be observed, due to the production of O-2 when endogenous SOD has been inactivated. Also, O-2 was converted to OH in an in vitro horseradish peroxidase (HRP)H2O2 system to which exogenous SOD has been added. Taken together with the discovery of the cell wall-bound Mn-SOD isoform, these results support the role of such a cell wall-bound SOD in the formation of OH jointly with the cell wall-bound POD. According to the above findings, it seems that the hydroxycinnamic acids from the cell wall, acting as reductants, contribute to the formation of H2O2 in the presence of O-2 in an autocatalytic manner, and that POD and Mn-SOD coupled together generate OH from such H2O2.",
publisher = "Oxford Univ Press, Oxford",
journal = "Plant and Cell Physiology",
title = "Generation of Hydroxyl Radical in Isolated Pea Root Cell Wall, and the Role of Cell Wall-Bound Peroxidase, Mn-SOD and Phenolics in Their Production",
pages = "317-304",
number = "2",
volume = "50",
doi = "10.1093/pcp/pcn199"
}

Kukavica, B., Mojović, M., Vučinić, Ž., Maksimović, V., Takahama, U.,& Veljović-Jovanović, S.. (2009). Generation of Hydroxyl Radical in Isolated Pea Root Cell Wall, and the Role of Cell Wall-Bound Peroxidase, Mn-SOD and Phenolics in Their Production. in Plant and Cell Physiology
Oxford Univ Press, Oxford., 50(2), 304-317.
https://doi.org/10.1093/pcp/pcn199

Kukavica B, Mojović M, Vučinić Ž, Maksimović V, Takahama U, Veljović-Jovanović S. Generation of Hydroxyl Radical in Isolated Pea Root Cell Wall, and the Role of Cell Wall-Bound Peroxidase, Mn-SOD and Phenolics in Their Production. in Plant and Cell Physiology. 2009;50(2):304-317.
doi:10.1093/pcp/pcn199 .

Kukavica, Biljana, Mojović, Miloš, Vučinić, Željko, Maksimović, Vuk, Takahama, Umeo, Veljović-Jovanović, Sonja, "Generation of Hydroxyl Radical in Isolated Pea Root Cell Wall, and the Role of Cell Wall-Bound Peroxidase, Mn-SOD and Phenolics in Their Production" in Plant and Cell Physiology, 50, no. 2 (2009):304-317,
https://doi.org/10.1093/pcp/pcn199 . .

TY  - JOUR
AU  - Bogdanović Pristov, Jelena
AU  - Mojović, Miloš
AU  - Milosavić, N.
AU  - Mitrović, Aleksandra Lj.
AU  - Vučinić, Željko
AU  - Spasojević, Ivan
PY  - 2008
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/282
AB  - This work presents findings, which indicate important role of fructose, fructose 6-phosphate (F6P), and fructose 1,6-bisphosphate (FBP) in preservation of homeostasis in plants under low temperature. Cold combined with light is known to incite increased generation of superoxide in chloroplasts leading to photoinhibition, but also an increased level of soluble sugars. In the present study, oxidative stress in pea leaves provoked by cold/light regime was asserted by the observed decrease of the level of oxidized form of PSI pigment P700 (P700(+)). Alongside, the increased antioxidative status and the accumulation of fructose were observed. The antioxidative properties of fructose and its phosphorylated forms were evaluated to appraise their potential protective role in plants exposed to chilling stress. Fructose, and particularly F6P and FBP exhibited high capacities for scavenging superoxide and showed to be involved in antioxidative protection in pea leaves. These results combined with previously established links implicate that the increase in level of fructose sugars through various pathways intercalated into physiological mechanisms of homeostasis represents important non-enzymatic antioxidative defense in plants under cold-related stress.
PB  - Springer, New York
T2  - European Biophysics Journal With Biophysics Letters
T1  - Role of fructose in the adaptation of plants to cold-induced oxidative stress
EP  - 1246
IS  - 7
SP  - 1241
VL  - 37
DO  - 10.1007/s00249-008-0260-9
ER  - 

@article{
author = "Bogdanović Pristov, Jelena and Mojović, Miloš and Milosavić, N. and Mitrović, Aleksandra Lj. and Vučinić, Željko and Spasojević, Ivan",
year = "2008",
abstract = "This work presents findings, which indicate important role of fructose, fructose 6-phosphate (F6P), and fructose 1,6-bisphosphate (FBP) in preservation of homeostasis in plants under low temperature. Cold combined with light is known to incite increased generation of superoxide in chloroplasts leading to photoinhibition, but also an increased level of soluble sugars. In the present study, oxidative stress in pea leaves provoked by cold/light regime was asserted by the observed decrease of the level of oxidized form of PSI pigment P700 (P700(+)). Alongside, the increased antioxidative status and the accumulation of fructose were observed. The antioxidative properties of fructose and its phosphorylated forms were evaluated to appraise their potential protective role in plants exposed to chilling stress. Fructose, and particularly F6P and FBP exhibited high capacities for scavenging superoxide and showed to be involved in antioxidative protection in pea leaves. These results combined with previously established links implicate that the increase in level of fructose sugars through various pathways intercalated into physiological mechanisms of homeostasis represents important non-enzymatic antioxidative defense in plants under cold-related stress.",
publisher = "Springer, New York",
journal = "European Biophysics Journal With Biophysics Letters",
title = "Role of fructose in the adaptation of plants to cold-induced oxidative stress",
pages = "1246-1241",
number = "7",
volume = "37",
doi = "10.1007/s00249-008-0260-9"
}

Bogdanović Pristov, J., Mojović, M., Milosavić, N., Mitrović, A. Lj., Vučinić, Ž.,& Spasojević, I.. (2008). Role of fructose in the adaptation of plants to cold-induced oxidative stress. in European Biophysics Journal With Biophysics Letters
Springer, New York., 37(7), 1241-1246.
https://doi.org/10.1007/s00249-008-0260-9

Bogdanović Pristov J, Mojović M, Milosavić N, Mitrović AL, Vučinić Ž, Spasojević I. Role of fructose in the adaptation of plants to cold-induced oxidative stress. in European Biophysics Journal With Biophysics Letters. 2008;37(7):1241-1246.
doi:10.1007/s00249-008-0260-9 .

Bogdanović Pristov, Jelena, Mojović, Miloš, Milosavić, N., Mitrović, Aleksandra Lj., Vučinić, Željko, Spasojević, Ivan, "Role of fructose in the adaptation of plants to cold-induced oxidative stress" in European Biophysics Journal With Biophysics Letters, 37, no. 7 (2008):1241-1246,
https://doi.org/10.1007/s00249-008-0260-9 . .

TY  - JOUR
AU  - Harhaji, Lj.
AU  - Mijatović, Sanja
AU  - Maksimović-Ivanić, Danijela
AU  - Stojanović, I.
AU  - Momcilović, Miljana
AU  - Maksimović, Vuk
AU  - Tufegdžić, Srđan J.
AU  - Marjanović, Žaklina
AU  - Mostarica-Stojković, M.
AU  - Vučinić, Željko
AU  - Stosic-Grujicic, Stanislava
PY  - 2008
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/262
AB  - Numerous studies have shown immunostimulatory and anti-tumor effects of water and standardized aqueous ethanol extracts derived from the medicinal mushroom, Coriolus versicolor, but the biological activity of methanol extracts has not been examined so far. In the present study we investigated the anti-tumor effect of C versicolor methanol extract (which contains terpenoids and poly-phenols) on B16 mouse melanoma cells both in vitro and in vivo. In vitro treatment of the cells with the methanol extract (25-1600 mu g/ml) reduced melanoma cell viability in it dose-dependent manner. Furthermore, in the presence of the methanol extract (200 mu g/ml, concentration IC50) the proliferation of B16 cells was arrested in the G(0)/G(1) phase of the cell cycle, followed by both apoptotic and secondary necrotic cell death. In vivo methanol extract treatment (i.p. 50 mg/kg, for 14 days) inhibited tumor growth in C57BL/6 mice inoculated with syngeneic B16 tumor cells. Moreover, peritoneal macrophages collected 21 days after tumor implantation from methanol extract-treated animals exerted stronger tumoristatic activity ex vivo than macrophages from control melanoma-bearing rnice. Taken together, our results demonstrate that C. versicolor methanol extract exerts pronounced anti-melanoma activity, both directly through antiproliferative and cytotoxic effects on tumor cells and indirectly through promotion of macrophage anti-tumor activity.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Food and Chemical Toxicology
T1  - Anti-tumor effect of Coriolus versicolor methanol extract against mouse B16 melanoma cells: In vitro and in vivo study
EP  - 1833
IS  - 5
SP  - 1825
VL  - 46
DO  - 10.1016/j.fct.2008.01.027
ER  - 

@article{
author = "Harhaji, Lj. and Mijatović, Sanja and Maksimović-Ivanić, Danijela and Stojanović, I. and Momcilović, Miljana and Maksimović, Vuk and Tufegdžić, Srđan J. and Marjanović, Žaklina and Mostarica-Stojković, M. and Vučinić, Željko and Stosic-Grujicic, Stanislava",
year = "2008",
abstract = "Numerous studies have shown immunostimulatory and anti-tumor effects of water and standardized aqueous ethanol extracts derived from the medicinal mushroom, Coriolus versicolor, but the biological activity of methanol extracts has not been examined so far. In the present study we investigated the anti-tumor effect of C versicolor methanol extract (which contains terpenoids and poly-phenols) on B16 mouse melanoma cells both in vitro and in vivo. In vitro treatment of the cells with the methanol extract (25-1600 mu g/ml) reduced melanoma cell viability in it dose-dependent manner. Furthermore, in the presence of the methanol extract (200 mu g/ml, concentration IC50) the proliferation of B16 cells was arrested in the G(0)/G(1) phase of the cell cycle, followed by both apoptotic and secondary necrotic cell death. In vivo methanol extract treatment (i.p. 50 mg/kg, for 14 days) inhibited tumor growth in C57BL/6 mice inoculated with syngeneic B16 tumor cells. Moreover, peritoneal macrophages collected 21 days after tumor implantation from methanol extract-treated animals exerted stronger tumoristatic activity ex vivo than macrophages from control melanoma-bearing rnice. Taken together, our results demonstrate that C. versicolor methanol extract exerts pronounced anti-melanoma activity, both directly through antiproliferative and cytotoxic effects on tumor cells and indirectly through promotion of macrophage anti-tumor activity.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Food and Chemical Toxicology",
title = "Anti-tumor effect of Coriolus versicolor methanol extract against mouse B16 melanoma cells: In vitro and in vivo study",
pages = "1833-1825",
number = "5",
volume = "46",
doi = "10.1016/j.fct.2008.01.027"
}

Harhaji, Lj., Mijatović, S., Maksimović-Ivanić, D., Stojanović, I., Momcilović, M., Maksimović, V., Tufegdžić, S. J., Marjanović, Ž., Mostarica-Stojković, M., Vučinić, Ž.,& Stosic-Grujicic, S.. (2008). Anti-tumor effect of Coriolus versicolor methanol extract against mouse B16 melanoma cells: In vitro and in vivo study. in Food and Chemical Toxicology
Pergamon-Elsevier Science Ltd, Oxford., 46(5), 1825-1833.
https://doi.org/10.1016/j.fct.2008.01.027

Harhaji L, Mijatović S, Maksimović-Ivanić D, Stojanović I, Momcilović M, Maksimović V, Tufegdžić SJ, Marjanović Ž, Mostarica-Stojković M, Vučinić Ž, Stosic-Grujicic S. Anti-tumor effect of Coriolus versicolor methanol extract against mouse B16 melanoma cells: In vitro and in vivo study. in Food and Chemical Toxicology. 2008;46(5):1825-1833.
doi:10.1016/j.fct.2008.01.027 .

Harhaji, Lj., Mijatović, Sanja, Maksimović-Ivanić, Danijela, Stojanović, I., Momcilović, Miljana, Maksimović, Vuk, Tufegdžić, Srđan J., Marjanović, Žaklina, Mostarica-Stojković, M., Vučinić, Željko, Stosic-Grujicic, Stanislava, "Anti-tumor effect of Coriolus versicolor methanol extract against mouse B16 melanoma cells: In vitro and in vivo study" in Food and Chemical Toxicology, 46, no. 5 (2008):1825-1833,
https://doi.org/10.1016/j.fct.2008.01.027 . .

TY  - JOUR
AU  - Hadzi-Tasković Sukalović, Vesna
AU  - Vuletic, Mirjana
AU  - Vučinić, Željko
AU  - Veljović-Jovanović, Sonja
PY  - 2008
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/264
AB  - The rate of ascorbate and nicotinamide adenine dinucleotide plus hydrogen (NADH) cooxidation (i.e., their nonenzymic oxidation by peroxidase/H2O2-generated phenoxyl radicals of three hydroxycinnamates: caffeate, ferulate and p-coumarate) was studied in vitro. The reactions initiated by different sources of peroxidase (EC 1.11.1.7) [isolates from soybean (Glycine max L.) seed coat, maize (Zea mays L.) root-cell wall, and commercial horseradish peroxidase] were monitored. Native electrophoresis of samples and specific staining for peroxidase activity revealed various isoforms in each of the three enzyme sources. The peroxidase sources differed both in the rate of H2O2-dependent hydroxycinnamate oxidation and in the order of affinity for the phenolic substrates. The three hydroxycinnamates did not differ in their ability to cooxidize ascorbate, whereas NADH cooxidation was affected by substitution of the phenolic ring. Thus, p-coumarate was more efficient than caffeate in NADH cooxidation, with ferulate not being effective at all. Metal ions (Zn2+ and Al3+) inhibited the reaction of peroxidase with p-coumarate and affected the cooxidation rate of ascorbate and the peroxidase reaction in the same manner with all substrates used. However, inhibition of p-coumarate oxidation by metal ions did not affect NADH cooxidation rate. We propose that both the ascorbate and NADH cooxidation systems can function as mechanisms to scavenge H2O2 and regenerate phenolics in different cellular compartments, thus contributing to protection from oxidative damage.
PB  - Springer Japan Kk, Tokyo
T2  - Journal of Plant Research
T1  - Effectiveness of phenoxyl radicals generated by peroxidase/H2O2-catalyzed oxidation of caffeate, ferulate, and p-coumarate in cooxidation of ascorbate and NADH
EP  - 123
IS  - 1
SP  - 115
VL  - 121
DO  - 10.1007/s10265-007-0124-x
ER  - 

@article{
author = "Hadzi-Tasković Sukalović, Vesna and Vuletic, Mirjana and Vučinić, Željko and Veljović-Jovanović, Sonja",
year = "2008",
abstract = "The rate of ascorbate and nicotinamide adenine dinucleotide plus hydrogen (NADH) cooxidation (i.e., their nonenzymic oxidation by peroxidase/H2O2-generated phenoxyl radicals of three hydroxycinnamates: caffeate, ferulate and p-coumarate) was studied in vitro. The reactions initiated by different sources of peroxidase (EC 1.11.1.7) [isolates from soybean (Glycine max L.) seed coat, maize (Zea mays L.) root-cell wall, and commercial horseradish peroxidase] were monitored. Native electrophoresis of samples and specific staining for peroxidase activity revealed various isoforms in each of the three enzyme sources. The peroxidase sources differed both in the rate of H2O2-dependent hydroxycinnamate oxidation and in the order of affinity for the phenolic substrates. The three hydroxycinnamates did not differ in their ability to cooxidize ascorbate, whereas NADH cooxidation was affected by substitution of the phenolic ring. Thus, p-coumarate was more efficient than caffeate in NADH cooxidation, with ferulate not being effective at all. Metal ions (Zn2+ and Al3+) inhibited the reaction of peroxidase with p-coumarate and affected the cooxidation rate of ascorbate and the peroxidase reaction in the same manner with all substrates used. However, inhibition of p-coumarate oxidation by metal ions did not affect NADH cooxidation rate. We propose that both the ascorbate and NADH cooxidation systems can function as mechanisms to scavenge H2O2 and regenerate phenolics in different cellular compartments, thus contributing to protection from oxidative damage.",
publisher = "Springer Japan Kk, Tokyo",
journal = "Journal of Plant Research",
title = "Effectiveness of phenoxyl radicals generated by peroxidase/H2O2-catalyzed oxidation of caffeate, ferulate, and p-coumarate in cooxidation of ascorbate and NADH",
pages = "123-115",
number = "1",
volume = "121",
doi = "10.1007/s10265-007-0124-x"
}

Hadzi-Tasković Sukalović, V., Vuletic, M., Vučinić, Ž.,& Veljović-Jovanović, S.. (2008). Effectiveness of phenoxyl radicals generated by peroxidase/H2O2-catalyzed oxidation of caffeate, ferulate, and p-coumarate in cooxidation of ascorbate and NADH. in Journal of Plant Research
Springer Japan Kk, Tokyo., 121(1), 115-123.
https://doi.org/10.1007/s10265-007-0124-x

Hadzi-Tasković Sukalović V, Vuletic M, Vučinić Ž, Veljović-Jovanović S. Effectiveness of phenoxyl radicals generated by peroxidase/H2O2-catalyzed oxidation of caffeate, ferulate, and p-coumarate in cooxidation of ascorbate and NADH. in Journal of Plant Research. 2008;121(1):115-123.
doi:10.1007/s10265-007-0124-x .

Hadzi-Tasković Sukalović, Vesna, Vuletic, Mirjana, Vučinić, Željko, Veljović-Jovanović, Sonja, "Effectiveness of phenoxyl radicals generated by peroxidase/H2O2-catalyzed oxidation of caffeate, ferulate, and p-coumarate in cooxidation of ascorbate and NADH" in Journal of Plant Research, 121, no. 1 (2008):115-123,
https://doi.org/10.1007/s10265-007-0124-x . .

TY  - JOUR
AU  - Cvetic, T.
AU  - Veljović-Jovanović, Sonja
AU  - Vučinić, Željko
PY  - 2008
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/243
AB  - Spinach leaves were used to extract isoforms of NAD-dependent malate dehydrogenase (NAD-MDH) (EC 1.1.1.37), either soluble or bound to microsomal, plasma, or chloroplast envelope membranes. All fractions were subjected to isoelectric focusing analysis, which showed that purified chloroplast envelopes contain an NAD-MDH isoform tightly bound to the membranes, since treatment with 0.5 or 1% Triton X-100 was not able to release the enzyme from the envelopes. In contrast, plasma membranes released an isoform with a pI of 3.5 following treatment with 0.5% Triton X-100. The most abundant soluble leaf isoform had a pI of 9, while the chloroplast stroma contained an isoform with a pI of 5.3. Kinetic analysis of oxaloacetate (OAA)-dependent NADH oxidation in different fractions gave different K-m values for both substrates, the envelope- and plasma membrane-bound NAD-MDH exhibiting the highest affinities for OAA. Leaf plasma membrane-bound MDH exhibited a high capacity for both reaction directions (malate oxidation and OAA reduction), while the two chloroplast isoforms (stromal and envelope-bound) preferentially reduced OAA. Our results indicate that the chloroplast envelope contains a specifically attached NAD-MDH isoform that could provide direct coupling between chloroplast and cytosol adenylate pools.
PB  - Springer Wien, Wien
T2  - Protoplasma
T1  - Characterization of NAD-dependent malate dehydrogenases from spinach leaves
EP  - 253
IS  - 3-4
SP  - 247
VL  - 232
DO  - 10.1007/s00709-007-0282-7
ER  - 

@article{
author = "Cvetic, T. and Veljović-Jovanović, Sonja and Vučinić, Željko",
year = "2008",
abstract = "Spinach leaves were used to extract isoforms of NAD-dependent malate dehydrogenase (NAD-MDH) (EC 1.1.1.37), either soluble or bound to microsomal, plasma, or chloroplast envelope membranes. All fractions were subjected to isoelectric focusing analysis, which showed that purified chloroplast envelopes contain an NAD-MDH isoform tightly bound to the membranes, since treatment with 0.5 or 1% Triton X-100 was not able to release the enzyme from the envelopes. In contrast, plasma membranes released an isoform with a pI of 3.5 following treatment with 0.5% Triton X-100. The most abundant soluble leaf isoform had a pI of 9, while the chloroplast stroma contained an isoform with a pI of 5.3. Kinetic analysis of oxaloacetate (OAA)-dependent NADH oxidation in different fractions gave different K-m values for both substrates, the envelope- and plasma membrane-bound NAD-MDH exhibiting the highest affinities for OAA. Leaf plasma membrane-bound MDH exhibited a high capacity for both reaction directions (malate oxidation and OAA reduction), while the two chloroplast isoforms (stromal and envelope-bound) preferentially reduced OAA. Our results indicate that the chloroplast envelope contains a specifically attached NAD-MDH isoform that could provide direct coupling between chloroplast and cytosol adenylate pools.",
publisher = "Springer Wien, Wien",
journal = "Protoplasma",
title = "Characterization of NAD-dependent malate dehydrogenases from spinach leaves",
pages = "253-247",
number = "3-4",
volume = "232",
doi = "10.1007/s00709-007-0282-7"
}

Cvetic, T., Veljović-Jovanović, S.,& Vučinić, Ž.. (2008). Characterization of NAD-dependent malate dehydrogenases from spinach leaves. in Protoplasma
Springer Wien, Wien., 232(3-4), 247-253.
https://doi.org/10.1007/s00709-007-0282-7

Cvetic T, Veljović-Jovanović S, Vučinić Ž. Characterization of NAD-dependent malate dehydrogenases from spinach leaves. in Protoplasma. 2008;232(3-4):247-253.
doi:10.1007/s00709-007-0282-7 .

Cvetic, T., Veljović-Jovanović, Sonja, Vučinić, Željko, "Characterization of NAD-dependent malate dehydrogenases from spinach leaves" in Protoplasma, 232, no. 3-4 (2008):247-253,
https://doi.org/10.1007/s00709-007-0282-7 . .

TY  - JOUR
AU  - Maksimović, Vuk
AU  - Mojović, Miloš
AU  - Vučinić, Željko
PY  - 2006
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/173
AB  - An analysis of the H2O2-induced breakdown and transformation of different keto-monosaccharides at physiological concentrations reveals that glycolate and other short-chained carbohydrates and organic acids are produced. Depletion of monosaccharides. and glycolate synthesis occurs at increased rates as the length of the carbohydrate chain is decreased, and is significantly increased in the presence of trace amounts of Fe2+ ions (10 mu M). Rates of monosaccharide depletion (initial concentration of 3 mM) observed were up to 1.55 mmol h(-1) in the case of fructose, and 2.59 mmol h(-1) in the case of dihydroxyacetone, depending upon pH, H2O2 concentration, temperature and the presence or absence of catalytic amounts of Fe2+. Glycolate was produced by dihydroxyacetone cleavage at rates up to 0.45 mmol h(-1) in the absence, and up to 1.88 mmol h(-1) in the presence of Fe2+ ions (pH 8). Besides glycolate, other sugars (ribose, glyceraldehyde, glucose), glucitol (sorbitol) and organic acids (formic and 2-oxogluconic acid) were produced in such H2O2-induced reactions with fructose or dihydroxyacetone. EPR measurements demonstrated the participation of the (OH)-O-. radical, especially at higher pH Presence of metal ions at higher pH values, resulting in increased glycolate synthesis, was accompanied by enhanced hydroxyl radical generation. Observed changes in intensity of DEPMPO-OH signals recorded from dihydroxyacetone and fructose reactions demonstrate a strong correlation with changes in glycolate yield, suggesting that (OH)-O-. radical formation enhances glycolate synthesis. The results presented suggest that different mechanisms are responsible for the cleavage or other reactions (isomerisation, auto- or free-radical-mediated oxidation) of keto-monosaccharides depending of experimental conditions.
PB  - Elsevier Sci Ltd, Oxford
T2  - Carbohydrate Research
T1  - Monosaccharide-H2O2 reactions as a source of glycolate and their stimulation by hydroxyl radicals
EP  - 2369
IS  - 14
SP  - 2360
VL  - 341
DO  - 10.1016/j.carres.2006.06.023
ER  - 

@article{
author = "Maksimović, Vuk and Mojović, Miloš and Vučinić, Željko",
year = "2006",
abstract = "An analysis of the H2O2-induced breakdown and transformation of different keto-monosaccharides at physiological concentrations reveals that glycolate and other short-chained carbohydrates and organic acids are produced. Depletion of monosaccharides. and glycolate synthesis occurs at increased rates as the length of the carbohydrate chain is decreased, and is significantly increased in the presence of trace amounts of Fe2+ ions (10 mu M). Rates of monosaccharide depletion (initial concentration of 3 mM) observed were up to 1.55 mmol h(-1) in the case of fructose, and 2.59 mmol h(-1) in the case of dihydroxyacetone, depending upon pH, H2O2 concentration, temperature and the presence or absence of catalytic amounts of Fe2+. Glycolate was produced by dihydroxyacetone cleavage at rates up to 0.45 mmol h(-1) in the absence, and up to 1.88 mmol h(-1) in the presence of Fe2+ ions (pH 8). Besides glycolate, other sugars (ribose, glyceraldehyde, glucose), glucitol (sorbitol) and organic acids (formic and 2-oxogluconic acid) were produced in such H2O2-induced reactions with fructose or dihydroxyacetone. EPR measurements demonstrated the participation of the (OH)-O-. radical, especially at higher pH Presence of metal ions at higher pH values, resulting in increased glycolate synthesis, was accompanied by enhanced hydroxyl radical generation. Observed changes in intensity of DEPMPO-OH signals recorded from dihydroxyacetone and fructose reactions demonstrate a strong correlation with changes in glycolate yield, suggesting that (OH)-O-. radical formation enhances glycolate synthesis. The results presented suggest that different mechanisms are responsible for the cleavage or other reactions (isomerisation, auto- or free-radical-mediated oxidation) of keto-monosaccharides depending of experimental conditions.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Carbohydrate Research",
title = "Monosaccharide-H2O2 reactions as a source of glycolate and their stimulation by hydroxyl radicals",
pages = "2369-2360",
number = "14",
volume = "341",
doi = "10.1016/j.carres.2006.06.023"
}

Maksimović, V., Mojović, M.,& Vučinić, Ž.. (2006). Monosaccharide-H2O2 reactions as a source of glycolate and their stimulation by hydroxyl radicals. in Carbohydrate Research
Elsevier Sci Ltd, Oxford., 341(14), 2360-2369.
https://doi.org/10.1016/j.carres.2006.06.023

Maksimović V, Mojović M, Vučinić Ž. Monosaccharide-H2O2 reactions as a source of glycolate and their stimulation by hydroxyl radicals. in Carbohydrate Research. 2006;341(14):2360-2369.
doi:10.1016/j.carres.2006.06.023 .

Maksimović, Vuk, Mojović, Miloš, Vučinić, Željko, "Monosaccharide-H2O2 reactions as a source of glycolate and their stimulation by hydroxyl radicals" in Carbohydrate Research, 341, no. 14 (2006):2360-2369,
https://doi.org/10.1016/j.carres.2006.06.023 . .

TY  - JOUR
AU  - Tufegdžić, Srđan J.
AU  - Bogdanović Pristov, Jelena
AU  - Maksimović, Vuk
AU  - Vučinić, Željko
PY  - 2005
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/137
AB  - Horseradish peroxidase was used to synthesize diferulates by a procedure in which ethyl ferulate was used as substrate. Four different forms were obtained, of which two dominant were the 5-5' and 8-5' diferulate. Fluorescence emission spectra of the diferulates (excited at 284 nm) indicate that they contain two chromophores, as opposed to the substrate molecule. Fluorescence excitation spectra with emission at 417 run further demonstrate the difference between the synthesized diferulates and starting substrates.
PB  - New York Acad Sciences, New York
T2  - Biophysics From Molecules to Brain: in Memory of Radoslav K. Andjus
T1  - Characterization of enzymatically synthesized diferulate
EP  - 470
SP  - 466
VL  - 1048
DO  - 10.1196/annals.1342.068
ER  - 

@article{
author = "Tufegdžić, Srđan J. and Bogdanović Pristov, Jelena and Maksimović, Vuk and Vučinić, Željko",
year = "2005",
abstract = "Horseradish peroxidase was used to synthesize diferulates by a procedure in which ethyl ferulate was used as substrate. Four different forms were obtained, of which two dominant were the 5-5' and 8-5' diferulate. Fluorescence emission spectra of the diferulates (excited at 284 nm) indicate that they contain two chromophores, as opposed to the substrate molecule. Fluorescence excitation spectra with emission at 417 run further demonstrate the difference between the synthesized diferulates and starting substrates.",
publisher = "New York Acad Sciences, New York",
journal = "Biophysics From Molecules to Brain: in Memory of Radoslav K. Andjus",
title = "Characterization of enzymatically synthesized diferulate",
pages = "470-466",
volume = "1048",
doi = "10.1196/annals.1342.068"
}

Tufegdžić, S. J., Bogdanović Pristov, J., Maksimović, V.,& Vučinić, Ž.. (2005). Characterization of enzymatically synthesized diferulate. in Biophysics From Molecules to Brain: in Memory of Radoslav K. Andjus
New York Acad Sciences, New York., 1048, 466-470.
https://doi.org/10.1196/annals.1342.068

Tufegdžić SJ, Bogdanović Pristov J, Maksimović V, Vučinić Ž. Characterization of enzymatically synthesized diferulate. in Biophysics From Molecules to Brain: in Memory of Radoslav K. Andjus. 2005;1048:466-470.
doi:10.1196/annals.1342.068 .

Tufegdžić, Srđan J., Bogdanović Pristov, Jelena, Maksimović, Vuk, Vučinić, Željko, "Characterization of enzymatically synthesized diferulate" in Biophysics From Molecules to Brain: in Memory of Radoslav K. Andjus, 1048 (2005):466-470,
https://doi.org/10.1196/annals.1342.068 . .

TY  - JOUR
AU  - Maksimović, Vuk
AU  - Mojović, Miloš
AU  - Neumann, G
AU  - Vučinić, Željko
PY  - 2005
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/130
AB  - An analysis of the reaction of dihydroxyacetone with hydrogen peroxide was performed using HPLC with electrochemical and spectrophotometric detection and EPR spectroscopy of the reactants and products. Glycolate production in such a reaction has been documented, and the glycolate yield analyzed at different pH values. The participation of hydroxyl free radical is shown with DEPMPO spin trap. Two types of mechanisms are proposed, one at higher pH values and pronounced participation of free radicals (enhanced by the presence of metal ions and Fenton reaction) and the other at lower pH values, with decreased free radical production and lower glycolate yield.
PB  - New York Acad Sciences, New York
T2  - Biophysics From Molecules to Brain: in Memory of Radoslav K. Andjus
T1  - Nonenzymatic reaction of dihydroxyacetone with hydrogen peroxide enhanced via a Fenton reaction
EP  - 465
SP  - 461
VL  - 1048
DO  - 10.1196/annals.1342.067
ER  - 

@article{
author = "Maksimović, Vuk and Mojović, Miloš and Neumann, G and Vučinić, Željko",
year = "2005",
abstract = "An analysis of the reaction of dihydroxyacetone with hydrogen peroxide was performed using HPLC with electrochemical and spectrophotometric detection and EPR spectroscopy of the reactants and products. Glycolate production in such a reaction has been documented, and the glycolate yield analyzed at different pH values. The participation of hydroxyl free radical is shown with DEPMPO spin trap. Two types of mechanisms are proposed, one at higher pH values and pronounced participation of free radicals (enhanced by the presence of metal ions and Fenton reaction) and the other at lower pH values, with decreased free radical production and lower glycolate yield.",
publisher = "New York Acad Sciences, New York",
journal = "Biophysics From Molecules to Brain: in Memory of Radoslav K. Andjus",
title = "Nonenzymatic reaction of dihydroxyacetone with hydrogen peroxide enhanced via a Fenton reaction",
pages = "465-461",
volume = "1048",
doi = "10.1196/annals.1342.067"
}

Maksimović, V., Mojović, M., Neumann, G.,& Vučinić, Ž.. (2005). Nonenzymatic reaction of dihydroxyacetone with hydrogen peroxide enhanced via a Fenton reaction. in Biophysics From Molecules to Brain: in Memory of Radoslav K. Andjus
New York Acad Sciences, New York., 1048, 461-465.
https://doi.org/10.1196/annals.1342.067

Maksimović V, Mojović M, Neumann G, Vučinić Ž. Nonenzymatic reaction of dihydroxyacetone with hydrogen peroxide enhanced via a Fenton reaction. in Biophysics From Molecules to Brain: in Memory of Radoslav K. Andjus. 2005;1048:461-465.
doi:10.1196/annals.1342.067 .

Maksimović, Vuk, Mojović, Miloš, Neumann, G, Vučinić, Željko, "Nonenzymatic reaction of dihydroxyacetone with hydrogen peroxide enhanced via a Fenton reaction" in Biophysics From Molecules to Brain: in Memory of Radoslav K. Andjus, 1048 (2005):461-465,
https://doi.org/10.1196/annals.1342.067 . .

TY  - JOUR
AU  - Mojović, Miloš
AU  - Spasojević, Ivan
AU  - Vuletic, M
AU  - Vučinić, Željko
AU  - Bačić, Goran
PY  - 2005
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/142
AB  - Plant plasma membranes are known to produce superoxide radicals, while the production of hydroxyl radical is thought to occur only in the cell wall. In this work it was demonstrated using combined spin-trap and spin-probe EPR spectroscopic techniques, that plant plasma membranes do produce superoxide and hydroxyl radicals but by kinetically different mechanisms. The results show that superoxide and hydroxyl radicals can be detected by DMPO spin-trap and that the mechanisms and location of their production call be differentiated using the reduction of spin-probes Tempone and 7-DS. It was shown that the mechanism of production of oxygen reactive species is NADH dependent and diphenylene iodonium inhibited. The kinetics of the reduction of Temponc, combined with scavengers or the absence of NADH indicates that hydroxyl radicals are produced by a mechanism independent of that of superoxide production. It was shown that a combination of the spill-probe and spin-trap technique can be used in free radical studies of biological systems, with a number of advantages inherent to them.
PB  - Srpsko hemijsko društvo, Beograd
T2  - Journal of the Serbian Chemical Society
T1  - An EPR spin-probe and spin-trap study of the free radicals produced by plant plasma membranes
EP  - 186
IS  - 2
SP  - 177
VL  - 70
DO  - 10.2298/JSC0502177M
ER  - 

@article{
author = "Mojović, Miloš and Spasojević, Ivan and Vuletic, M and Vučinić, Željko and Bačić, Goran",
year = "2005",
abstract = "Plant plasma membranes are known to produce superoxide radicals, while the production of hydroxyl radical is thought to occur only in the cell wall. In this work it was demonstrated using combined spin-trap and spin-probe EPR spectroscopic techniques, that plant plasma membranes do produce superoxide and hydroxyl radicals but by kinetically different mechanisms. The results show that superoxide and hydroxyl radicals can be detected by DMPO spin-trap and that the mechanisms and location of their production call be differentiated using the reduction of spin-probes Tempone and 7-DS. It was shown that the mechanism of production of oxygen reactive species is NADH dependent and diphenylene iodonium inhibited. The kinetics of the reduction of Temponc, combined with scavengers or the absence of NADH indicates that hydroxyl radicals are produced by a mechanism independent of that of superoxide production. It was shown that a combination of the spill-probe and spin-trap technique can be used in free radical studies of biological systems, with a number of advantages inherent to them.",
publisher = "Srpsko hemijsko društvo, Beograd",
journal = "Journal of the Serbian Chemical Society",
title = "An EPR spin-probe and spin-trap study of the free radicals produced by plant plasma membranes",
pages = "186-177",
number = "2",
volume = "70",
doi = "10.2298/JSC0502177M"
}

Mojović, M., Spasojević, I., Vuletic, M., Vučinić, Ž.,& Bačić, G.. (2005). An EPR spin-probe and spin-trap study of the free radicals produced by plant plasma membranes. in Journal of the Serbian Chemical Society
Srpsko hemijsko društvo, Beograd., 70(2), 177-186.
https://doi.org/10.2298/JSC0502177M

Mojović M, Spasojević I, Vuletic M, Vučinić Ž, Bačić G. An EPR spin-probe and spin-trap study of the free radicals produced by plant plasma membranes. in Journal of the Serbian Chemical Society. 2005;70(2):177-186.
doi:10.2298/JSC0502177M .

Mojović, Miloš, Spasojević, Ivan, Vuletic, M, Vučinić, Željko, Bačić, Goran, "An EPR spin-probe and spin-trap study of the free radicals produced by plant plasma membranes" in Journal of the Serbian Chemical Society, 70, no. 2 (2005):177-186,
https://doi.org/10.2298/JSC0502177M . .

TY  - JOUR
AU  - Veljović-Jovanović, Sonja
AU  - Kukavica, Biljana
AU  - Cvetic, T
AU  - Mojović, Miloš
AU  - Vučinić, Željko
PY  - 2005
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/138
AB  - A comparative fluorescence and oxygen radical-sensitive spin trap EPR spectroscopic study of isolated cell walls (with proteins or deproteinated), in the presence and absence of ascorbate and H2O2 is presented. Fluorescence spectra indicate the presence of at least two fluorophores, one degraded and the other synthesized after reduction or oxidation, indicating phenol di/polymerization. DEPMPO spin trap measurements show that isolated cell walls are capable of oxygen-dependent hydroxyl radical generation in the absence of NADH or other reductants, ascorbate addition, or deproteination of the cell wall abolishing the signal due to hydroxyl radicals.
PB  - New York Acad Sciences, New York
T2  - Biophysics From Molecules to Brain: in Memory of Radoslav K. Andjus
T1  - Ascorbic acid and the oxidative processes in pea root cell wall isolates - Characterization by fluorescence and EPR spectroscopy
EP  - 504
SP  - 500
VL  - 1048
DO  - 10.1196/annals.1342.076
ER  - 

@article{
author = "Veljović-Jovanović, Sonja and Kukavica, Biljana and Cvetic, T and Mojović, Miloš and Vučinić, Željko",
year = "2005",
abstract = "A comparative fluorescence and oxygen radical-sensitive spin trap EPR spectroscopic study of isolated cell walls (with proteins or deproteinated), in the presence and absence of ascorbate and H2O2 is presented. Fluorescence spectra indicate the presence of at least two fluorophores, one degraded and the other synthesized after reduction or oxidation, indicating phenol di/polymerization. DEPMPO spin trap measurements show that isolated cell walls are capable of oxygen-dependent hydroxyl radical generation in the absence of NADH or other reductants, ascorbate addition, or deproteination of the cell wall abolishing the signal due to hydroxyl radicals.",
publisher = "New York Acad Sciences, New York",
journal = "Biophysics From Molecules to Brain: in Memory of Radoslav K. Andjus",
title = "Ascorbic acid and the oxidative processes in pea root cell wall isolates - Characterization by fluorescence and EPR spectroscopy",
pages = "504-500",
volume = "1048",
doi = "10.1196/annals.1342.076"
}

Veljović-Jovanović, S., Kukavica, B., Cvetic, T., Mojović, M.,& Vučinić, Ž.. (2005). Ascorbic acid and the oxidative processes in pea root cell wall isolates - Characterization by fluorescence and EPR spectroscopy. in Biophysics From Molecules to Brain: in Memory of Radoslav K. Andjus
New York Acad Sciences, New York., 1048, 500-504.
https://doi.org/10.1196/annals.1342.076

Veljović-Jovanović S, Kukavica B, Cvetic T, Mojović M, Vučinić Ž. Ascorbic acid and the oxidative processes in pea root cell wall isolates - Characterization by fluorescence and EPR spectroscopy. in Biophysics From Molecules to Brain: in Memory of Radoslav K. Andjus. 2005;1048:500-504.
doi:10.1196/annals.1342.076 .

Veljović-Jovanović, Sonja, Kukavica, Biljana, Cvetic, T, Mojović, Miloš, Vučinić, Željko, "Ascorbic acid and the oxidative processes in pea root cell wall isolates - Characterization by fluorescence and EPR spectroscopy" in Biophysics From Molecules to Brain: in Memory of Radoslav K. Andjus, 1048 (2005):500-504,
https://doi.org/10.1196/annals.1342.076 . .

TY  - JOUR
AU  - Vuletic, M
AU  - Radotić, Ksenija
AU  - Vučinić, Željko
PY  - 1998
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/39
AB  - The identity of the naturally occurring compounds that accept electrons from plasma membrane-bound redox systems in vivo is obscure. We analysed the effect of ascorbate, oxygen, iron, as well as their free radical forms, and also the free radical-generating and -quenching systems on the trans-root electrical potential, which had previously been shown to be coupled to plasma membrane-bound redox systems. The material was the primary root of 8-day-old maize (Zea mays L.) seedlings. Trans-root electrical potential difference was measured across excised roots. Different ascorbate (ascorbate, dehydroascorbate and ascorbate free radical) and oxygen redox forms (superoxide and hydroxide radicals and hydrogen peroxide), as well as scavenging agents of oxygen species (superoxide dismutase, catalase, mannitol), and ferric and ferrous ions were added to the solution flowing around the root. Ascorbate free radical induced the greatest depolarization of the trans-root potential when compared to other ascorbate redox forms, which is consistent with its suggested role as a natural electron acceptor. Addition of xanthine oxidase, with or without xanthine, also produced depolarizing effects. The presence of SOD magnified this effect both with ascorbate free radical and xanthine oxidase. When ferric or ferrous chloride and ferric EDTA were applied to the bathing medium, only free ferric ion produced a very pronounced depolarization. The magnitude and kinetics of trans-root potential depolarization, induced by the ascorbate redox forms and systems for the generation and scavenging of oxygen species, argue in favour of the mutually competing electron transfer role of ascorbate free radicals and superoxide radicals in the extracellular space of the root. These results provide evidence that at least a part of the electrical potential difference occurring across plant roots arises from current flow from the symplast, via the plasma membrane-bound redox systems, to naturally occurring compounds in the apoplast, and that this transfer is achieved through the mediation of their free radical forms.
PB  - Wiley, Hoboken
T2  - Physiologia Plantarum
T1  - Free radical involvement in the generation of trans-root potential
EP  - 782
IS  - 4
SP  - 777
VL  - 104
DO  - 10.1034/j.1399-3054.1998.1040438.x
ER  - 

@article{
author = "Vuletic, M and Radotić, Ksenija and Vučinić, Željko",
year = "1998",
abstract = "The identity of the naturally occurring compounds that accept electrons from plasma membrane-bound redox systems in vivo is obscure. We analysed the effect of ascorbate, oxygen, iron, as well as their free radical forms, and also the free radical-generating and -quenching systems on the trans-root electrical potential, which had previously been shown to be coupled to plasma membrane-bound redox systems. The material was the primary root of 8-day-old maize (Zea mays L.) seedlings. Trans-root electrical potential difference was measured across excised roots. Different ascorbate (ascorbate, dehydroascorbate and ascorbate free radical) and oxygen redox forms (superoxide and hydroxide radicals and hydrogen peroxide), as well as scavenging agents of oxygen species (superoxide dismutase, catalase, mannitol), and ferric and ferrous ions were added to the solution flowing around the root. Ascorbate free radical induced the greatest depolarization of the trans-root potential when compared to other ascorbate redox forms, which is consistent with its suggested role as a natural electron acceptor. Addition of xanthine oxidase, with or without xanthine, also produced depolarizing effects. The presence of SOD magnified this effect both with ascorbate free radical and xanthine oxidase. When ferric or ferrous chloride and ferric EDTA were applied to the bathing medium, only free ferric ion produced a very pronounced depolarization. The magnitude and kinetics of trans-root potential depolarization, induced by the ascorbate redox forms and systems for the generation and scavenging of oxygen species, argue in favour of the mutually competing electron transfer role of ascorbate free radicals and superoxide radicals in the extracellular space of the root. These results provide evidence that at least a part of the electrical potential difference occurring across plant roots arises from current flow from the symplast, via the plasma membrane-bound redox systems, to naturally occurring compounds in the apoplast, and that this transfer is achieved through the mediation of their free radical forms.",
publisher = "Wiley, Hoboken",
journal = "Physiologia Plantarum",
title = "Free radical involvement in the generation of trans-root potential",
pages = "782-777",
number = "4",
volume = "104",
doi = "10.1034/j.1399-3054.1998.1040438.x"
}

Vuletic, M., Radotić, K.,& Vučinić, Ž.. (1998). Free radical involvement in the generation of trans-root potential. in Physiologia Plantarum
Wiley, Hoboken., 104(4), 777-782.
https://doi.org/10.1034/j.1399-3054.1998.1040438.x

Vuletic M, Radotić K, Vučinić Ž. Free radical involvement in the generation of trans-root potential. in Physiologia Plantarum. 1998;104(4):777-782.
doi:10.1034/j.1399-3054.1998.1040438.x .

Vuletic, M, Radotić, Ksenija, Vučinić, Željko, "Free radical involvement in the generation of trans-root potential" in Physiologia Plantarum, 104, no. 4 (1998):777-782,
https://doi.org/10.1034/j.1399-3054.1998.1040438.x . .