TY  - CONF
AU  - Lukicic, J.
AU  - Rodic, I.
AU  - Žižić, Milan
AU  - Zakrzewska, Joanna
AU  - Cvetić-Antić, Tijana
AU  - Zivić, Miroslav
AU  - Stanić, Marina
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1463
AB  - Fungi represent the main route of vanadium’s entrance in ecosystem and are known to tolerate high concentrations of this transition metal. Despite it, its effects on antioxidant systems of fungi are not well-known. The aim of this research was to examine the effects of vanadate on antioxidant enzymes and non-enzymatic antioxidants of filamentous fungus P. blakesleeanus. Mycelium in early exponential (20h) and stationary (56h) phase of growth was treated with relatively high concentrations of V+5 (1, 5 and 10 mM) for 1h or 5h. Activities of peroxidase (POD), glutathione reductase (GR), glutathione transferase (GST), glutathione peroxidase (GPx), concentrations of total phenols and total glutathione were examined. In the 20h old mycelia, V5+ in all concentrations, after 1h treatments, induced decrease in total phenols with the largest decrease (25 ± 4%) after 10mM V5+ treatment, while in 56h old mycelia decrease in total phenols was noticed only after 1 mM V+5 treatment (11 ± 3%). Decrease of total glutathione, in 56h old mycelia, was noticed for all concentrations after 1h treatments, and the largest 38 ± 7% was induced by 5 mM V5+. The same effect was noticed in 20h old mycelia treated for 5h, with the largest decrease of 29 ± 7% after 5 mM V5+ treatment. In 56h old mycelia, after 1h treatments with 10 mM V+5, activities of GPx and POD increased, while after 5h treatments, concentration dependent increase in activities of GST, GPx and GR was noticed, with the largest increase of 112 ± 30% for GPx, 74 ± 39% for GST and 68 ± 26% for GR. Increase in the activities of GPx and GST was noticed in 20h old mycelia treated for 5h, with 5 mM and 10 mM V+5 and also increase of POD in 56h old mycelia, was noticed in same treatments, but the only statistically significant increase was after treatment with 10 mMV+5 in 56h old mycelia (43 ± 10%). The results shown indicate that after 1h treatments, V5+ influenced primarily on non-enzymatic antioxidants and after 5h treatments on enzymes.
PB  - Wiley, Hoboken
C3  - Febs Open Bio
T1  - Effects of vanadate on antioxidant systems in mycelium of fungus Phycomyces blakesleeanus
EP  - 397
SP  - 396
VL  - 11
DO  - 10.1002/2211-5463.13205
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1463
ER  - 

@conference{
author = "Lukicic, J. and Rodic, I. and Žižić, Milan and Zakrzewska, Joanna and Cvetić-Antić, Tijana and Zivić, Miroslav and Stanić, Marina",
year = "2021",
abstract = "Fungi represent the main route of vanadium’s entrance in ecosystem and are known to tolerate high concentrations of this transition metal. Despite it, its effects on antioxidant systems of fungi are not well-known. The aim of this research was to examine the effects of vanadate on antioxidant enzymes and non-enzymatic antioxidants of filamentous fungus P. blakesleeanus. Mycelium in early exponential (20h) and stationary (56h) phase of growth was treated with relatively high concentrations of V+5 (1, 5 and 10 mM) for 1h or 5h. Activities of peroxidase (POD), glutathione reductase (GR), glutathione transferase (GST), glutathione peroxidase (GPx), concentrations of total phenols and total glutathione were examined. In the 20h old mycelia, V5+ in all concentrations, after 1h treatments, induced decrease in total phenols with the largest decrease (25 ± 4%) after 10mM V5+ treatment, while in 56h old mycelia decrease in total phenols was noticed only after 1 mM V+5 treatment (11 ± 3%). Decrease of total glutathione, in 56h old mycelia, was noticed for all concentrations after 1h treatments, and the largest 38 ± 7% was induced by 5 mM V5+. The same effect was noticed in 20h old mycelia treated for 5h, with the largest decrease of 29 ± 7% after 5 mM V5+ treatment. In 56h old mycelia, after 1h treatments with 10 mM V+5, activities of GPx and POD increased, while after 5h treatments, concentration dependent increase in activities of GST, GPx and GR was noticed, with the largest increase of 112 ± 30% for GPx, 74 ± 39% for GST and 68 ± 26% for GR. Increase in the activities of GPx and GST was noticed in 20h old mycelia treated for 5h, with 5 mM and 10 mM V+5 and also increase of POD in 56h old mycelia, was noticed in same treatments, but the only statistically significant increase was after treatment with 10 mMV+5 in 56h old mycelia (43 ± 10%). The results shown indicate that after 1h treatments, V5+ influenced primarily on non-enzymatic antioxidants and after 5h treatments on enzymes.",
publisher = "Wiley, Hoboken",
journal = "Febs Open Bio",
title = "Effects of vanadate on antioxidant systems in mycelium of fungus Phycomyces blakesleeanus",
pages = "397-396",
volume = "11",
doi = "10.1002/2211-5463.13205",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1463"
}

Lukicic, J., Rodic, I., Žižić, M., Zakrzewska, J., Cvetić-Antić, T., Zivić, M.,& Stanić, M.. (2021). Effects of vanadate on antioxidant systems in mycelium of fungus Phycomyces blakesleeanus. in Febs Open Bio
Wiley, Hoboken., 11, 396-397.
https://doi.org/10.1002/2211-5463.13205
https://hdl.handle.net/21.15107/rcub_rimsi_1463

Lukicic J, Rodic I, Žižić M, Zakrzewska J, Cvetić-Antić T, Zivić M, Stanić M. Effects of vanadate on antioxidant systems in mycelium of fungus Phycomyces blakesleeanus. in Febs Open Bio. 2021;11:396-397.
doi:10.1002/2211-5463.13205
https://hdl.handle.net/21.15107/rcub_rimsi_1463 .

Lukicic, J., Rodic, I., Žižić, Milan, Zakrzewska, Joanna, Cvetić-Antić, Tijana, Zivić, Miroslav, Stanić, Marina, "Effects of vanadate on antioxidant systems in mycelium of fungus Phycomyces blakesleeanus" in Febs Open Bio, 11 (2021):396-397,
https://doi.org/10.1002/2211-5463.13205 .,
https://hdl.handle.net/21.15107/rcub_rimsi_1463 .

TY  - CONF
AU  - Žižić, Milan
AU  - Macura, Melanija
AU  - Zakrzewska, Joanna
AU  - Cvetić-Antić, Tijana
AU  - Zivić, Miroslav
AU  - Stanić, Marina
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1470
AB  - Selenium represents an essential microelement for many organisms, and exists in
several oxidation states. Selenite (Se4+) is often toxic due to its water solubility and bioavailability,
but can be reduced to insoluble Se0 by many microorganisms including fungi. Fungus
Phycomyces blakesleeanus reduces Se4+ to Se0 and forms selenium nanoparticles (BioSeNPs) as
witnessed by red coloration of the mycelium after 24h of treatment with 2, 5 and 10 mM Se4+. SEM
micrographics and EDS spectra confirmed presence of BioSeNPs with characteristic peaks at 1.4
(SeLα), 11.2 (SeKα) and 12.5 (SeKβ) keV. One of the mechanisms of selenite toxicity is considered
to be production of reactive oxygen species that then triggers response of cellular antioxidative
systems, among which important part is played by thiols such as glutathione. Glutathione is also
believed to play a more direct role in reduction of Se4+ to Se0 as proposed by the Painter reaction.
Thiol specific biradical EPR probe (RSSR) was used for EPR in vivo detection of intracellular thiolgroup modifications in mycelium of fungus P. blakesleanus treated by 0.1, 0.5, 1, 2, 5 and 10 mM
selenite (Na2SeO3) for 24 h. Decrease of available –SH groups is detected as the decrease in ratio
of mono- and biradical peak in the spectrum of the given sample. This ratio was 43.05 for control,
and didn’t change with 0.1 mM treatment (42.23), but halved to 23.55 with 0.5 mM Se4+. It further
decreased 10 × to minimal values of 4.31, 4.21, 4.1 and 3.5 with 1, 2, 5 and 10 mM Se4+, respectively.
This cannot be solely attributed to biomass decrease as it never decreased more than 45%. P.
blakesleeanus forms BioSeNPs from Se4+ and intracellular thiols are involved in this process
either through neutralization of ROS or directly in the reduction of Se4+, but most probably both.
PB  - Elsevier Science Inc, New York
C3  - Free Radical Biology and Medicine
T1  - Production of BioSeNPs in fungus Phycomyces blakesleanus is accompanied by decrease in intracellular thiols as detected by in vivo EPR
VL  - 177
DO  - 10.1016/j.freeradbiomed.2021.08.204
ER  - 

@conference{
author = "Žižić, Milan and Macura, Melanija and Zakrzewska, Joanna and Cvetić-Antić, Tijana and Zivić, Miroslav and Stanić, Marina",
year = "2021",
abstract = "Selenium represents an essential microelement for many organisms, and exists in
several oxidation states. Selenite (Se4+) is often toxic due to its water solubility and bioavailability,
but can be reduced to insoluble Se0 by many microorganisms including fungi. Fungus
Phycomyces blakesleeanus reduces Se4+ to Se0 and forms selenium nanoparticles (BioSeNPs) as
witnessed by red coloration of the mycelium after 24h of treatment with 2, 5 and 10 mM Se4+. SEM
micrographics and EDS spectra confirmed presence of BioSeNPs with characteristic peaks at 1.4
(SeLα), 11.2 (SeKα) and 12.5 (SeKβ) keV. One of the mechanisms of selenite toxicity is considered
to be production of reactive oxygen species that then triggers response of cellular antioxidative
systems, among which important part is played by thiols such as glutathione. Glutathione is also
believed to play a more direct role in reduction of Se4+ to Se0 as proposed by the Painter reaction.
Thiol specific biradical EPR probe (RSSR) was used for EPR in vivo detection of intracellular thiolgroup modifications in mycelium of fungus P. blakesleanus treated by 0.1, 0.5, 1, 2, 5 and 10 mM
selenite (Na2SeO3) for 24 h. Decrease of available –SH groups is detected as the decrease in ratio
of mono- and biradical peak in the spectrum of the given sample. This ratio was 43.05 for control,
and didn’t change with 0.1 mM treatment (42.23), but halved to 23.55 with 0.5 mM Se4+. It further
decreased 10 × to minimal values of 4.31, 4.21, 4.1 and 3.5 with 1, 2, 5 and 10 mM Se4+, respectively.
This cannot be solely attributed to biomass decrease as it never decreased more than 45%. P.
blakesleeanus forms BioSeNPs from Se4+ and intracellular thiols are involved in this process
either through neutralization of ROS or directly in the reduction of Se4+, but most probably both.",
publisher = "Elsevier Science Inc, New York",
journal = "Free Radical Biology and Medicine",
title = "Production of BioSeNPs in fungus Phycomyces blakesleanus is accompanied by decrease in intracellular thiols as detected by in vivo EPR",
volume = "177",
doi = "10.1016/j.freeradbiomed.2021.08.204"
}

Žižić, M., Macura, M., Zakrzewska, J., Cvetić-Antić, T., Zivić, M.,& Stanić, M.. (2021). Production of BioSeNPs in fungus Phycomyces blakesleanus is accompanied by decrease in intracellular thiols as detected by in vivo EPR. in Free Radical Biology and Medicine
Elsevier Science Inc, New York., 177.
https://doi.org/10.1016/j.freeradbiomed.2021.08.204

Žižić M, Macura M, Zakrzewska J, Cvetić-Antić T, Zivić M, Stanić M. Production of BioSeNPs in fungus Phycomyces blakesleanus is accompanied by decrease in intracellular thiols as detected by in vivo EPR. in Free Radical Biology and Medicine. 2021;177.
doi:10.1016/j.freeradbiomed.2021.08.204 .

Žižić, Milan, Macura, Melanija, Zakrzewska, Joanna, Cvetić-Antić, Tijana, Zivić, Miroslav, Stanić, Marina, "Production of BioSeNPs in fungus Phycomyces blakesleanus is accompanied by decrease in intracellular thiols as detected by in vivo EPR" in Free Radical Biology and Medicine, 177 (2021),
https://doi.org/10.1016/j.freeradbiomed.2021.08.204 . .

TY  - JOUR
AU  - Avdović, Edina H.
AU  - Petrović, Isidora P.
AU  - Stevanović, Milena J.
AU  - Saso, Luciano
AU  - Dimitrić-Marković, Jasmina
AU  - Filipović, Nenad D.
AU  - Zivić, Miroslav
AU  - Cvetic, Antic, Tijana N.
AU  - Žižić, Milan
AU  - Todorović, Nataša
AU  - Vukic, Milena
AU  - Trifunović, Srecko R.
AU  - Marković, Zoran S.
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1457
AB  - Two newly synthesized 4-hydroxycoumarin bidentate ligands (L1 and L2) and their palladium(II) complexes (C1 and C2) were screened for their biological activities, in vitro and in vivo. Structures of new compounds were established based on elemental analysis, H-1 NMR, C-13 NMR, and IR spectroscopic techniques. The obtained compounds were tested for their antioxidative and cytotoxic activities and results pointed to selective antiradical activity of palladium(II) complexes towards (OH)-O-center dot and -center dot OOH radicals and anti-ABTS (2,2 '-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) cation radical) activity comparable to that of ascorbate. Results indicated the effect of C1 and C2 on the enzymatic activity of the antioxidative defense system. In vitro cytotoxicity assay performed on different carcinoma cell lines (HCT166, A375, and MIA PaCa-2), and one healthy fibroblast cell line (MRC-5) showed a cytotoxic effect of both C1 and C2, expressed as a decrease in carcinoma cells' viability, mostly by induction of apoptosis. In vivo toxicity tests performed on zebrafish embryos indicated different effects of C1 and C2, ranging from adverse developmental effect to no toxicity, depending on tested concentration. According to docking studies, both complexes (C1 and C2) showed better inhibitory activity in comparison to other palladium(II) complexes.
PB  - Hindawi Ltd, London
T2  - Oxidative Medicine and Cellular Longevity
T1  - Synthesis and Biological Screening of New 4-Hydroxycoumarin Derivatives and Their Palladium(II) Complexes
VL  - 2021
DO  - 10.1155/2021/8849568
ER  - 

@article{
author = "Avdović, Edina H. and Petrović, Isidora P. and Stevanović, Milena J. and Saso, Luciano and Dimitrić-Marković, Jasmina and Filipović, Nenad D. and Zivić, Miroslav and Cvetic, Antic, Tijana N. and Žižić, Milan and Todorović, Nataša and Vukic, Milena and Trifunović, Srecko R. and Marković, Zoran S.",
year = "2021",
abstract = "Two newly synthesized 4-hydroxycoumarin bidentate ligands (L1 and L2) and their palladium(II) complexes (C1 and C2) were screened for their biological activities, in vitro and in vivo. Structures of new compounds were established based on elemental analysis, H-1 NMR, C-13 NMR, and IR spectroscopic techniques. The obtained compounds were tested for their antioxidative and cytotoxic activities and results pointed to selective antiradical activity of palladium(II) complexes towards (OH)-O-center dot and -center dot OOH radicals and anti-ABTS (2,2 '-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) cation radical) activity comparable to that of ascorbate. Results indicated the effect of C1 and C2 on the enzymatic activity of the antioxidative defense system. In vitro cytotoxicity assay performed on different carcinoma cell lines (HCT166, A375, and MIA PaCa-2), and one healthy fibroblast cell line (MRC-5) showed a cytotoxic effect of both C1 and C2, expressed as a decrease in carcinoma cells' viability, mostly by induction of apoptosis. In vivo toxicity tests performed on zebrafish embryos indicated different effects of C1 and C2, ranging from adverse developmental effect to no toxicity, depending on tested concentration. According to docking studies, both complexes (C1 and C2) showed better inhibitory activity in comparison to other palladium(II) complexes.",
publisher = "Hindawi Ltd, London",
journal = "Oxidative Medicine and Cellular Longevity",
title = "Synthesis and Biological Screening of New 4-Hydroxycoumarin Derivatives and Their Palladium(II) Complexes",
volume = "2021",
doi = "10.1155/2021/8849568"
}

Avdović, E. H., Petrović, I. P., Stevanović, M. J., Saso, L., Dimitrić-Marković, J., Filipović, N. D., Zivić, M., Cvetic, A. T. N., Žižić, M., Todorović, N., Vukic, M., Trifunović, S. R.,& Marković, Z. S.. (2021). Synthesis and Biological Screening of New 4-Hydroxycoumarin Derivatives and Their Palladium(II) Complexes. in Oxidative Medicine and Cellular Longevity
Hindawi Ltd, London., 2021.
https://doi.org/10.1155/2021/8849568

Avdović EH, Petrović IP, Stevanović MJ, Saso L, Dimitrić-Marković J, Filipović ND, Zivić M, Cvetic ATN, Žižić M, Todorović N, Vukic M, Trifunović SR, Marković ZS. Synthesis and Biological Screening of New 4-Hydroxycoumarin Derivatives and Their Palladium(II) Complexes. in Oxidative Medicine and Cellular Longevity. 2021;2021.
doi:10.1155/2021/8849568 .

Avdović, Edina H., Petrović, Isidora P., Stevanović, Milena J., Saso, Luciano, Dimitrić-Marković, Jasmina, Filipović, Nenad D., Zivić, Miroslav, Cvetic, Antic, Tijana N., Žižić, Milan, Todorović, Nataša, Vukic, Milena, Trifunović, Srecko R., Marković, Zoran S., "Synthesis and Biological Screening of New 4-Hydroxycoumarin Derivatives and Their Palladium(II) Complexes" in Oxidative Medicine and Cellular Longevity, 2021 (2021),
https://doi.org/10.1155/2021/8849568 . .

TY  - JOUR
AU  - Cvetić-Antić, Tijana
AU  - Janosević, Dusica
AU  - Maksimović, Vuk
AU  - Zivić, Miroslav
AU  - Budimir, Snežana M
AU  - Glamočlija, Jasmina
AU  - Mitrović, Aleksandra Lj.
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1383
AB  - We present changes in Tacitus bellus antioxidative system that specifically correspond to subsequent phases of hemibiotroph Fusarium verticillioides infection revealed by histological analysis. T. bellus response to spore germination 6 h post inoculation (hpi), manifested as first oxidative burst, was characterized by transient decrease in malondialdehyde (MDA) content, transient increase in catalase (CAT), low level of superoxide dismutase (SOD) and peroxidase (POD) activity, as well as with transient decrease in total antioxidant capacity (TAC), total phenol content (TPC) and phenylalanine ammonium lyase activity (PAL), and no changes in polyphenol oxidase (PPO) activity, or phenolic profile. During the biotrophic phase of F. verticillioides infection, characterized by hyphae spread intercellularly in epidermal and mesophyll tissue, the host antioxidative system was suppressed. The transition to necrotrophic phase of F. verticillioides infection (inter- and intracellular colonization and sporulation), occurred 3-4 days post inoculation (dpi). During the necrotrophic phase, 5-7 dpi, slowed progression of colonization of T. bellus mesophyll cells occurred and it coincided with sharp increase in MDA content and CAT, SOD and POD activities, but the drop in TAC, TPC content, and PPO activity, as well as the production of phytotoxin fusaric acid. Presented results add to the knowledge of events and mechanisms related to the transition from biotrophy to necrotrophy in F. verticillioides.
PB  - Elsevier Gmbh, Munich
T2  - Journal of Plant Physiology
T1  - Biochemical and histological characterization of succulent plant Tacitus bellus response to Fusarium verticillioides infection in vitro
VL  - 244
DO  - 10.1016/j.jplph.2019.153086
ER  - 

@article{
author = "Cvetić-Antić, Tijana and Janosević, Dusica and Maksimović, Vuk and Zivić, Miroslav and Budimir, Snežana M and Glamočlija, Jasmina and Mitrović, Aleksandra Lj.",
year = "2020",
abstract = "We present changes in Tacitus bellus antioxidative system that specifically correspond to subsequent phases of hemibiotroph Fusarium verticillioides infection revealed by histological analysis. T. bellus response to spore germination 6 h post inoculation (hpi), manifested as first oxidative burst, was characterized by transient decrease in malondialdehyde (MDA) content, transient increase in catalase (CAT), low level of superoxide dismutase (SOD) and peroxidase (POD) activity, as well as with transient decrease in total antioxidant capacity (TAC), total phenol content (TPC) and phenylalanine ammonium lyase activity (PAL), and no changes in polyphenol oxidase (PPO) activity, or phenolic profile. During the biotrophic phase of F. verticillioides infection, characterized by hyphae spread intercellularly in epidermal and mesophyll tissue, the host antioxidative system was suppressed. The transition to necrotrophic phase of F. verticillioides infection (inter- and intracellular colonization and sporulation), occurred 3-4 days post inoculation (dpi). During the necrotrophic phase, 5-7 dpi, slowed progression of colonization of T. bellus mesophyll cells occurred and it coincided with sharp increase in MDA content and CAT, SOD and POD activities, but the drop in TAC, TPC content, and PPO activity, as well as the production of phytotoxin fusaric acid. Presented results add to the knowledge of events and mechanisms related to the transition from biotrophy to necrotrophy in F. verticillioides.",
publisher = "Elsevier Gmbh, Munich",
journal = "Journal of Plant Physiology",
title = "Biochemical and histological characterization of succulent plant Tacitus bellus response to Fusarium verticillioides infection in vitro",
volume = "244",
doi = "10.1016/j.jplph.2019.153086"
}

Cvetić-Antić, T., Janosević, D., Maksimović, V., Zivić, M., Budimir, S. M., Glamočlija, J.,& Mitrović, A. Lj.. (2020). Biochemical and histological characterization of succulent plant Tacitus bellus response to Fusarium verticillioides infection in vitro. in Journal of Plant Physiology
Elsevier Gmbh, Munich., 244.
https://doi.org/10.1016/j.jplph.2019.153086

Cvetić-Antić T, Janosević D, Maksimović V, Zivić M, Budimir SM, Glamočlija J, Mitrović AL. Biochemical and histological characterization of succulent plant Tacitus bellus response to Fusarium verticillioides infection in vitro. in Journal of Plant Physiology. 2020;244.
doi:10.1016/j.jplph.2019.153086 .

Cvetić-Antić, Tijana, Janosević, Dusica, Maksimović, Vuk, Zivić, Miroslav, Budimir, Snežana M, Glamočlija, Jasmina, Mitrović, Aleksandra Lj., "Biochemical and histological characterization of succulent plant Tacitus bellus response to Fusarium verticillioides infection in vitro" in Journal of Plant Physiology, 244 (2020),
https://doi.org/10.1016/j.jplph.2019.153086 . .

TY  - JOUR
AU  - Chatzopoulou, Fani
AU  - Sanmartin, Maite
AU  - Mellidou, Ifigeneia
AU  - Pateraki, Irini
AU  - Koukounaras, Athanasios
AU  - Tanou, Georgia
AU  - Kalamaki, Mary S.
AU  - Veljović-Jovanović, Sonja
AU  - Cvetić-Antić, Tijana
AU  - Kostas, Stefanos
AU  - Tsouvaltzis, Pavlos
AU  - Grumet, Rebecca
AU  - Kanellis, Angelos K.
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1327
AB  - Ascorbate oxidase (AO, EC 1.10.3.3) is a copper-containing enzyme localized at the apoplast, where it catalyzes the oxidation of ascorbic acid (AA) to dehydroascorbic acid (DHA) via monodehydroascorbic acid (MDHA) intermediate. Despite it has been extensively studied, no biological roles have been definitively ascribed. To understand the role of AO in plant metabolism, fruit growth and physiology, we suppressed AO expression in melon (Cucumis melo L.) fruit. Reduction of AO activity increased AA content in melon fruit, which is the result of repression of AA oxidation and simultaneous induction of certain biosynthetic and recycling genes. As a consequence, ascorbate redox state was altered in the apoplast. Interestingly, transgenic melon fruit displayed increased ethylene production rate coincided with elevated levels of 1-aminocyclopropane-1-carboxylic acid (ACC) oxidase (ACO, EC 1.14.17.4) activity and gene expression, which might contribute to earlier ripening. Moreover, AO suppressed transgenic melon fruit exhibited a dramatic arrest in fruit growth, due to a simultaneous decrease in fruit cell size and in plasmalemma (PM) ATPase activity. All the above, support for the first time, the in vivo AO participation in the rapid fruit growth of Cucurbitaceae and further suggest an alternative route for AA increase in ripening fruit.
PB  - Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux
T2  - Plant Physiology and Biochemistry
T1  - Silencing of ascorbate oxidase results in reduced growth, altered ascorbic acid levels and ripening pattern in melon fruit
EP  - 303
SP  - 291
VL  - 156
DO  - 10.1016/j.plaphy.2020.08.040
ER  - 

@article{
author = "Chatzopoulou, Fani and Sanmartin, Maite and Mellidou, Ifigeneia and Pateraki, Irini and Koukounaras, Athanasios and Tanou, Georgia and Kalamaki, Mary S. and Veljović-Jovanović, Sonja and Cvetić-Antić, Tijana and Kostas, Stefanos and Tsouvaltzis, Pavlos and Grumet, Rebecca and Kanellis, Angelos K.",
year = "2020",
abstract = "Ascorbate oxidase (AO, EC 1.10.3.3) is a copper-containing enzyme localized at the apoplast, where it catalyzes the oxidation of ascorbic acid (AA) to dehydroascorbic acid (DHA) via monodehydroascorbic acid (MDHA) intermediate. Despite it has been extensively studied, no biological roles have been definitively ascribed. To understand the role of AO in plant metabolism, fruit growth and physiology, we suppressed AO expression in melon (Cucumis melo L.) fruit. Reduction of AO activity increased AA content in melon fruit, which is the result of repression of AA oxidation and simultaneous induction of certain biosynthetic and recycling genes. As a consequence, ascorbate redox state was altered in the apoplast. Interestingly, transgenic melon fruit displayed increased ethylene production rate coincided with elevated levels of 1-aminocyclopropane-1-carboxylic acid (ACC) oxidase (ACO, EC 1.14.17.4) activity and gene expression, which might contribute to earlier ripening. Moreover, AO suppressed transgenic melon fruit exhibited a dramatic arrest in fruit growth, due to a simultaneous decrease in fruit cell size and in plasmalemma (PM) ATPase activity. All the above, support for the first time, the in vivo AO participation in the rapid fruit growth of Cucurbitaceae and further suggest an alternative route for AA increase in ripening fruit.",
publisher = "Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux",
journal = "Plant Physiology and Biochemistry",
title = "Silencing of ascorbate oxidase results in reduced growth, altered ascorbic acid levels and ripening pattern in melon fruit",
pages = "303-291",
volume = "156",
doi = "10.1016/j.plaphy.2020.08.040"
}

Chatzopoulou, F., Sanmartin, M., Mellidou, I., Pateraki, I., Koukounaras, A., Tanou, G., Kalamaki, M. S., Veljović-Jovanović, S., Cvetić-Antić, T., Kostas, S., Tsouvaltzis, P., Grumet, R.,& Kanellis, A. K.. (2020). Silencing of ascorbate oxidase results in reduced growth, altered ascorbic acid levels and ripening pattern in melon fruit. in Plant Physiology and Biochemistry
Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux., 156, 291-303.
https://doi.org/10.1016/j.plaphy.2020.08.040

Chatzopoulou F, Sanmartin M, Mellidou I, Pateraki I, Koukounaras A, Tanou G, Kalamaki MS, Veljović-Jovanović S, Cvetić-Antić T, Kostas S, Tsouvaltzis P, Grumet R, Kanellis AK. Silencing of ascorbate oxidase results in reduced growth, altered ascorbic acid levels and ripening pattern in melon fruit. in Plant Physiology and Biochemistry. 2020;156:291-303.
doi:10.1016/j.plaphy.2020.08.040 .

Chatzopoulou, Fani, Sanmartin, Maite, Mellidou, Ifigeneia, Pateraki, Irini, Koukounaras, Athanasios, Tanou, Georgia, Kalamaki, Mary S., Veljović-Jovanović, Sonja, Cvetić-Antić, Tijana, Kostas, Stefanos, Tsouvaltzis, Pavlos, Grumet, Rebecca, Kanellis, Angelos K., "Silencing of ascorbate oxidase results in reduced growth, altered ascorbic acid levels and ripening pattern in melon fruit" in Plant Physiology and Biochemistry, 156 (2020):291-303,
https://doi.org/10.1016/j.plaphy.2020.08.040 . .

TY  - JOUR
AU  - Stanić, Marina
AU  - Krizak, Strahinja
AU  - Jovanović, Mirna
AU  - Pajic, Tanja
AU  - Ciric, Ana
AU  - Žižić, Milan
AU  - Zakrzewska, Joanna
AU  - Cvetić-Antić, Tijana
AU  - Todorović, Nataša
AU  - Zivić, Miroslav
PY  - 2017
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1082
AB  - Increasing resistance of fungal strains to known fungicides has prompted identification of new candidates for fungicides among substances previously used for other purposes. We have tested the effects of known anion channel inhibitors anthracene-9-carboxylic acid (A9C) and niflumic acid (NFA) on growth, energy metabolism and anionic current of mycelium of fungus Phycomyces blakesleeanus. Both inhibitors significantly decreased growth and respiration of mycelium, but complete inhibition was only achieved by 100 and 500 mu M NFA for growth and respiration, respectively. A9C had no effect on respiration of human NCI-H460 cell line and very little effect on cucumber root sprout clippings, which nominates this inhibitor for further investigation as a potential new fungicide. Effects of A9C and NFA on respiration of isolated mitochondria of P. blakesleeanus were significantly smaller, which indicates that their inhibitory effect on respiration of mycelium is indirect. NMR spectroscopy showed that both A9C and NFA decrease the levels of ATP and polyphosphates in the mycelium of P. blakesleeanus, but only A9C caused intracellular acidification. Outwardly rectifying, fast inactivating instantaneous anionic current (ORIC) was also reduced to 33 +/- 5 and 21 +/- 3% of its pre-treatment size by A9C and NFA, respectively, but only in the absence of ATP. It can be assumed from our results that the regulation of ORIC is tightly linked to cellular energy metabolism in P. blakesleeanus, and the decrease in ATP and polyphosphate levels could be a direct cause of growth inhibition.
PB  - Microbiology Soc, London
T2  - Microbiology-Sgm
T1  - Growth inhibition of fungus Phycomyces blakesleeanus by anion channel inhibitors anthracene-9-carboxylic and niflumic acid attained through decrease in cellular respiration and energy metabolites
EP  - 372
IS  - 3
SP  - 364
VL  - 163
DO  - 10.1099/mic.0.000429
ER  - 

@article{
author = "Stanić, Marina and Krizak, Strahinja and Jovanović, Mirna and Pajic, Tanja and Ciric, Ana and Žižić, Milan and Zakrzewska, Joanna and Cvetić-Antić, Tijana and Todorović, Nataša and Zivić, Miroslav",
year = "2017",
abstract = "Increasing resistance of fungal strains to known fungicides has prompted identification of new candidates for fungicides among substances previously used for other purposes. We have tested the effects of known anion channel inhibitors anthracene-9-carboxylic acid (A9C) and niflumic acid (NFA) on growth, energy metabolism and anionic current of mycelium of fungus Phycomyces blakesleeanus. Both inhibitors significantly decreased growth and respiration of mycelium, but complete inhibition was only achieved by 100 and 500 mu M NFA for growth and respiration, respectively. A9C had no effect on respiration of human NCI-H460 cell line and very little effect on cucumber root sprout clippings, which nominates this inhibitor for further investigation as a potential new fungicide. Effects of A9C and NFA on respiration of isolated mitochondria of P. blakesleeanus were significantly smaller, which indicates that their inhibitory effect on respiration of mycelium is indirect. NMR spectroscopy showed that both A9C and NFA decrease the levels of ATP and polyphosphates in the mycelium of P. blakesleeanus, but only A9C caused intracellular acidification. Outwardly rectifying, fast inactivating instantaneous anionic current (ORIC) was also reduced to 33 +/- 5 and 21 +/- 3% of its pre-treatment size by A9C and NFA, respectively, but only in the absence of ATP. It can be assumed from our results that the regulation of ORIC is tightly linked to cellular energy metabolism in P. blakesleeanus, and the decrease in ATP and polyphosphate levels could be a direct cause of growth inhibition.",
publisher = "Microbiology Soc, London",
journal = "Microbiology-Sgm",
title = "Growth inhibition of fungus Phycomyces blakesleeanus by anion channel inhibitors anthracene-9-carboxylic and niflumic acid attained through decrease in cellular respiration and energy metabolites",
pages = "372-364",
number = "3",
volume = "163",
doi = "10.1099/mic.0.000429"
}

Stanić, M., Krizak, S., Jovanović, M., Pajic, T., Ciric, A., Žižić, M., Zakrzewska, J., Cvetić-Antić, T., Todorović, N.,& Zivić, M.. (2017). Growth inhibition of fungus Phycomyces blakesleeanus by anion channel inhibitors anthracene-9-carboxylic and niflumic acid attained through decrease in cellular respiration and energy metabolites. in Microbiology-Sgm
Microbiology Soc, London., 163(3), 364-372.
https://doi.org/10.1099/mic.0.000429

Stanić M, Krizak S, Jovanović M, Pajic T, Ciric A, Žižić M, Zakrzewska J, Cvetić-Antić T, Todorović N, Zivić M. Growth inhibition of fungus Phycomyces blakesleeanus by anion channel inhibitors anthracene-9-carboxylic and niflumic acid attained through decrease in cellular respiration and energy metabolites. in Microbiology-Sgm. 2017;163(3):364-372.
doi:10.1099/mic.0.000429 .

Stanić, Marina, Krizak, Strahinja, Jovanović, Mirna, Pajic, Tanja, Ciric, Ana, Žižić, Milan, Zakrzewska, Joanna, Cvetić-Antić, Tijana, Todorović, Nataša, Zivić, Miroslav, "Growth inhibition of fungus Phycomyces blakesleeanus by anion channel inhibitors anthracene-9-carboxylic and niflumic acid attained through decrease in cellular respiration and energy metabolites" in Microbiology-Sgm, 163, no. 3 (2017):364-372,
https://doi.org/10.1099/mic.0.000429 . .

TY  - JOUR
AU  - Hadzibrahimović, Mirzeta
AU  - Sužnjević, Desanka
AU  - Pastor, Ferenc
AU  - Cvetić-Antić, Tijana
AU  - Žižić, Milan
AU  - Zakrzewska, Joanna
AU  - Zivić, Miroslav
PY  - 2017
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1064
AB  - The possibility of reduction of vanadate monomer in the mycelium of fungus Phycomyces blakesleeanus was investigated in this study by means of polarography. Control experiments were performed with vanadyl [V(IV)] and vanadate [V(V)] in 10 mM Hepes, pH 7.2. Addition of P. blakesleeanus mycelium resulted in disappearance of all V(IV) polarographic waves recorded in the control. This points to the uptake of all available V(IV) by the mycelium, up to 185 A mu mol/g(FW), and suggests P. blakesleeanus as a potential agent in V(IV) bioremediation. Polarographic measurements of mycelium with low concentrations (0.1-1 mM) of V(V), that only allows the presence of monomer, showed that fungal mycelia removes around 27% of V(V) from the extracellular solution. Uptake was saturated at 104 +/- 2 A mu mol/g(FW) which indicates excellent bioaccumulation capability of P. blakesleeanus. EPR, V-51 NMR and polarographic experiments showed no indications of any measurable extracellular complexation of V(V) monomer with fungal exudates, reduction by the mycelium or adsorption to the cell wall. Therefore, in contrast to vanadium oligomers, vanadate monomer interactions with the mycelium are restricted to its transport into the fungal cell, probably by a phosphate transporter.
PB  - Springer, Dordrecht
T2  - Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology
T1  - The interactions of vanadate monomer with the mycelium of fungus Phycomyces blakesleeanus: reduction or uptake?
EP  - 373
IS  - 3
SP  - 365
VL  - 110
DO  - 10.1007/s10482-016-0808-0
ER  - 

@article{
author = "Hadzibrahimović, Mirzeta and Sužnjević, Desanka and Pastor, Ferenc and Cvetić-Antić, Tijana and Žižić, Milan and Zakrzewska, Joanna and Zivić, Miroslav",
year = "2017",
abstract = "The possibility of reduction of vanadate monomer in the mycelium of fungus Phycomyces blakesleeanus was investigated in this study by means of polarography. Control experiments were performed with vanadyl [V(IV)] and vanadate [V(V)] in 10 mM Hepes, pH 7.2. Addition of P. blakesleeanus mycelium resulted in disappearance of all V(IV) polarographic waves recorded in the control. This points to the uptake of all available V(IV) by the mycelium, up to 185 A mu mol/g(FW), and suggests P. blakesleeanus as a potential agent in V(IV) bioremediation. Polarographic measurements of mycelium with low concentrations (0.1-1 mM) of V(V), that only allows the presence of monomer, showed that fungal mycelia removes around 27% of V(V) from the extracellular solution. Uptake was saturated at 104 +/- 2 A mu mol/g(FW) which indicates excellent bioaccumulation capability of P. blakesleeanus. EPR, V-51 NMR and polarographic experiments showed no indications of any measurable extracellular complexation of V(V) monomer with fungal exudates, reduction by the mycelium or adsorption to the cell wall. Therefore, in contrast to vanadium oligomers, vanadate monomer interactions with the mycelium are restricted to its transport into the fungal cell, probably by a phosphate transporter.",
publisher = "Springer, Dordrecht",
journal = "Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology",
title = "The interactions of vanadate monomer with the mycelium of fungus Phycomyces blakesleeanus: reduction or uptake?",
pages = "373-365",
number = "3",
volume = "110",
doi = "10.1007/s10482-016-0808-0"
}

Hadzibrahimović, M., Sužnjević, D., Pastor, F., Cvetić-Antić, T., Žižić, M., Zakrzewska, J.,& Zivić, M.. (2017). The interactions of vanadate monomer with the mycelium of fungus Phycomyces blakesleeanus: reduction or uptake?. in Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology
Springer, Dordrecht., 110(3), 365-373.
https://doi.org/10.1007/s10482-016-0808-0

Hadzibrahimović M, Sužnjević D, Pastor F, Cvetić-Antić T, Žižić M, Zakrzewska J, Zivić M. The interactions of vanadate monomer with the mycelium of fungus Phycomyces blakesleeanus: reduction or uptake?. in Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology. 2017;110(3):365-373.
doi:10.1007/s10482-016-0808-0 .

Hadzibrahimović, Mirzeta, Sužnjević, Desanka, Pastor, Ferenc, Cvetić-Antić, Tijana, Žižić, Milan, Zakrzewska, Joanna, Zivić, Miroslav, "The interactions of vanadate monomer with the mycelium of fungus Phycomyces blakesleeanus: reduction or uptake?" in Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology, 110, no. 3 (2017):365-373,
https://doi.org/10.1007/s10482-016-0808-0 . .

TY  - JOUR
AU  - Žižić, Milan
AU  - Zivić, Miroslav
AU  - Maksimović, Vuk
AU  - Stanić, Marina
AU  - Krizak, Strahinja
AU  - Cvetić-Antić, Tijana
AU  - Zakrzewska, Joanna
PY  - 2014
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/750
AB  - The biological and chemical basis of vanadium action in fungi is relatively poorly understood. In the present study, we investigate the influence of vanadate (V5+) on phosphate metabolism of Phycomyces blakesleeanus. Addition of V5+ caused increase of sugar phosphates signal intensities in P-31 NMR spectra in vivo. HPLC analysis of mycelial phosphate extracts demonstrated increased concentrations of glucose 6 phosphate, fructose 6 phosphate, fructose 1, 6 phosphate and glucose 1 phosphate after V5+ treatment. Influence of V5+ on the levels of fructose 2, 6 phosphate, glucosamine 6 phosphate and glucose 1, 6 phosphate (HPLC), and polyphosphates, UDPG and ATP (P-31 NMR) was also established. Increase of sugar phosphates content was not observed after addition of vanadyl (V4+), indicating that only vanadate influences its metabolism. Obtained results from in vivo experiments indicate catalytic/inhibitory vanadate action on enzymes involved in reactions of glycolysis and glycogenesis i.e., phosphoglucomutase, phosphofructokinase and glycogen phosphorylase in filamentous fungi.
PB  - Public Library Science, San Francisco
T2  - PLoS One
T1  - Vanadate Influence on Metabolism of Sugar Phosphates in Fungus Phycomyces blakesleeanus
IS  - 7
VL  - 9
DO  - 10.1371/journal.pone.0102849
ER  - 

@article{
author = "Žižić, Milan and Zivić, Miroslav and Maksimović, Vuk and Stanić, Marina and Krizak, Strahinja and Cvetić-Antić, Tijana and Zakrzewska, Joanna",
year = "2014",
abstract = "The biological and chemical basis of vanadium action in fungi is relatively poorly understood. In the present study, we investigate the influence of vanadate (V5+) on phosphate metabolism of Phycomyces blakesleeanus. Addition of V5+ caused increase of sugar phosphates signal intensities in P-31 NMR spectra in vivo. HPLC analysis of mycelial phosphate extracts demonstrated increased concentrations of glucose 6 phosphate, fructose 6 phosphate, fructose 1, 6 phosphate and glucose 1 phosphate after V5+ treatment. Influence of V5+ on the levels of fructose 2, 6 phosphate, glucosamine 6 phosphate and glucose 1, 6 phosphate (HPLC), and polyphosphates, UDPG and ATP (P-31 NMR) was also established. Increase of sugar phosphates content was not observed after addition of vanadyl (V4+), indicating that only vanadate influences its metabolism. Obtained results from in vivo experiments indicate catalytic/inhibitory vanadate action on enzymes involved in reactions of glycolysis and glycogenesis i.e., phosphoglucomutase, phosphofructokinase and glycogen phosphorylase in filamentous fungi.",
publisher = "Public Library Science, San Francisco",
journal = "PLoS One",
title = "Vanadate Influence on Metabolism of Sugar Phosphates in Fungus Phycomyces blakesleeanus",
number = "7",
volume = "9",
doi = "10.1371/journal.pone.0102849"
}

Žižić, M., Zivić, M., Maksimović, V., Stanić, M., Krizak, S., Cvetić-Antić, T.,& Zakrzewska, J.. (2014). Vanadate Influence on Metabolism of Sugar Phosphates in Fungus Phycomyces blakesleeanus. in PLoS One
Public Library Science, San Francisco., 9(7).
https://doi.org/10.1371/journal.pone.0102849

Žižić M, Zivić M, Maksimović V, Stanić M, Krizak S, Cvetić-Antić T, Zakrzewska J. Vanadate Influence on Metabolism of Sugar Phosphates in Fungus Phycomyces blakesleeanus. in PLoS One. 2014;9(7).
doi:10.1371/journal.pone.0102849 .

Žižić, Milan, Zivić, Miroslav, Maksimović, Vuk, Stanić, Marina, Krizak, Strahinja, Cvetić-Antić, Tijana, Zakrzewska, Joanna, "Vanadate Influence on Metabolism of Sugar Phosphates in Fungus Phycomyces blakesleeanus" in PLoS One, 9, no. 7 (2014),
https://doi.org/10.1371/journal.pone.0102849 . .

TY  - JOUR
AU  - Žižić, Milan
AU  - Zivić, Miroslav
AU  - Spasojević, Ivan
AU  - Bogdanović Pristov, Jelena
AU  - Stanić, Marina
AU  - Cvetić-Antić, Tijana
AU  - Zakrzewska, Joanna
PY  - 2013
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/632
AB  - The biological and chemical basis of vanadium action and transport in fungi is relatively poorly understood. In this study we investigated the interactions of vanadium in physiologically-relevant redox states: vanadate (+5) and vanadyl (+4), with mycelium of fungus Phycomyces blakesleeanus using EPR and P-31 NMR spectroscopy and biochemical assays. We determined that P. blakesleeanus reduces V5+ to V4+ in the extracellular compartment by the means of cell surface enzyme with ferricyanide reductase activity, which contains molybdenum molybdopterin as a cofactor. Both, V5+ and V4+ bind to cell wall. They enter the cytoplasm via phosphate transporter and cation channels, respectively, and exhibit different metabolic effects. Vanadate provokes increased biomass production, the effects being inverted to toxic at higher V5+ concentrations. In addition, V5+ activates the synthesis of sugar phosphates and oligophosphates. On the other hand, V4+ exhibits toxic effects even at low concentrations. The V4+ detoxification route involves binding to vacuolar polyphosphates. Altogether our results imply that the mechanism of interaction of vanadium with P. blakesleeanus involves three major steps: extracellular enzymatic V5+/V4+ reduction, V4+ influx, and vacuolar storage, with an additional step -V5+ import occurring at higher vanadate concentrations.
PB  - Elsevier Science Bv, Amsterdam
T2  - Research in Microbiology
T1  - The interactions of vanadium with Phycomyces blakesleeanus mycelium: enzymatic reduction, transport and metabolic effects
EP  - 69
IS  - 1
SP  - 61
VL  - 164
DO  - 10.1016/j.resmic.2012.08.007
ER  - 

@article{
author = "Žižić, Milan and Zivić, Miroslav and Spasojević, Ivan and Bogdanović Pristov, Jelena and Stanić, Marina and Cvetić-Antić, Tijana and Zakrzewska, Joanna",
year = "2013",
abstract = "The biological and chemical basis of vanadium action and transport in fungi is relatively poorly understood. In this study we investigated the interactions of vanadium in physiologically-relevant redox states: vanadate (+5) and vanadyl (+4), with mycelium of fungus Phycomyces blakesleeanus using EPR and P-31 NMR spectroscopy and biochemical assays. We determined that P. blakesleeanus reduces V5+ to V4+ in the extracellular compartment by the means of cell surface enzyme with ferricyanide reductase activity, which contains molybdenum molybdopterin as a cofactor. Both, V5+ and V4+ bind to cell wall. They enter the cytoplasm via phosphate transporter and cation channels, respectively, and exhibit different metabolic effects. Vanadate provokes increased biomass production, the effects being inverted to toxic at higher V5+ concentrations. In addition, V5+ activates the synthesis of sugar phosphates and oligophosphates. On the other hand, V4+ exhibits toxic effects even at low concentrations. The V4+ detoxification route involves binding to vacuolar polyphosphates. Altogether our results imply that the mechanism of interaction of vanadium with P. blakesleeanus involves three major steps: extracellular enzymatic V5+/V4+ reduction, V4+ influx, and vacuolar storage, with an additional step -V5+ import occurring at higher vanadate concentrations.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Research in Microbiology",
title = "The interactions of vanadium with Phycomyces blakesleeanus mycelium: enzymatic reduction, transport and metabolic effects",
pages = "69-61",
number = "1",
volume = "164",
doi = "10.1016/j.resmic.2012.08.007"
}

Žižić, M., Zivić, M., Spasojević, I., Bogdanović Pristov, J., Stanić, M., Cvetić-Antić, T.,& Zakrzewska, J.. (2013). The interactions of vanadium with Phycomyces blakesleeanus mycelium: enzymatic reduction, transport and metabolic effects. in Research in Microbiology
Elsevier Science Bv, Amsterdam., 164(1), 61-69.
https://doi.org/10.1016/j.resmic.2012.08.007

Žižić M, Zivić M, Spasojević I, Bogdanović Pristov J, Stanić M, Cvetić-Antić T, Zakrzewska J. The interactions of vanadium with Phycomyces blakesleeanus mycelium: enzymatic reduction, transport and metabolic effects. in Research in Microbiology. 2013;164(1):61-69.
doi:10.1016/j.resmic.2012.08.007 .

Žižić, Milan, Zivić, Miroslav, Spasojević, Ivan, Bogdanović Pristov, Jelena, Stanić, Marina, Cvetić-Antić, Tijana, Zakrzewska, Joanna, "The interactions of vanadium with Phycomyces blakesleeanus mycelium: enzymatic reduction, transport and metabolic effects" in Research in Microbiology, 164, no. 1 (2013):61-69,
https://doi.org/10.1016/j.resmic.2012.08.007 . .

TY  - JOUR
AU  - Zivić, Miroslav
AU  - Zakrzewska, Joanna
AU  - Stanić, Marina
AU  - Cvetic, T.
AU  - Živanović, Branka  D.
PY  - 2009
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/370
AB  - Respiratory characteristics of germinating spores, developing mycelium and mitochondria of the fungus Phycomyces blakesleeanus were investigated by means of oxygen Clark-type electrode. The effects of respiratory inhibitors and metabolic compounds on oxygen consumption were tested. It was demonstrated that P. blakesleeanus apart of cyanide-sensitive respiration, CSR, possess alternative respiration, (cyanide-resistant respiration, CRR) which is constitutive and whose capacity decreases during development. Maximum is observed for activated spores where CRR capacity is significantly greater than CSR. After treatment with antimycin A, a third type of respiration insensitive to antimycin A and low concentration of SHAM (sufficient for inhibition of CRR), but sensitive to cyanide and high concentration of SHAM, has been expressed.
PB  - Springer, Dordrecht
T2  - Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology
T1  - Alternative respiration of fungus Phycomyces blakesleeanus
EP  - 217
IS  - 3
SP  - 207
VL  - 95
DO  - 10.1007/s10482-008-9304-5
ER  - 

@article{
author = "Zivić, Miroslav and Zakrzewska, Joanna and Stanić, Marina and Cvetic, T. and Živanović, Branka  D.",
year = "2009",
abstract = "Respiratory characteristics of germinating spores, developing mycelium and mitochondria of the fungus Phycomyces blakesleeanus were investigated by means of oxygen Clark-type electrode. The effects of respiratory inhibitors and metabolic compounds on oxygen consumption were tested. It was demonstrated that P. blakesleeanus apart of cyanide-sensitive respiration, CSR, possess alternative respiration, (cyanide-resistant respiration, CRR) which is constitutive and whose capacity decreases during development. Maximum is observed for activated spores where CRR capacity is significantly greater than CSR. After treatment with antimycin A, a third type of respiration insensitive to antimycin A and low concentration of SHAM (sufficient for inhibition of CRR), but sensitive to cyanide and high concentration of SHAM, has been expressed.",
publisher = "Springer, Dordrecht",
journal = "Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology",
title = "Alternative respiration of fungus Phycomyces blakesleeanus",
pages = "217-207",
number = "3",
volume = "95",
doi = "10.1007/s10482-008-9304-5"
}

Zivić, M., Zakrzewska, J., Stanić, M., Cvetic, T.,& Živanović, Branka  D.. (2009). Alternative respiration of fungus Phycomyces blakesleeanus. in Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology
Springer, Dordrecht., 95(3), 207-217.
https://doi.org/10.1007/s10482-008-9304-5

Zivić M, Zakrzewska J, Stanić M, Cvetic T, Živanović, Branka  D.. Alternative respiration of fungus Phycomyces blakesleeanus. in Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology. 2009;95(3):207-217.
doi:10.1007/s10482-008-9304-5 .

Zivić, Miroslav, Zakrzewska, Joanna, Stanić, Marina, Cvetic, T., Živanović, Branka  D., "Alternative respiration of fungus Phycomyces blakesleeanus" in Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology, 95, no. 3 (2009):207-217,
https://doi.org/10.1007/s10482-008-9304-5 . .

TY  - JOUR
AU  - Zvezdanović, Jelena
AU  - Cvetic, Tijana
AU  - Veljović-Jovanović, Sonja
AU  - Marković, Dejan
PY  - 2009
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/310
AB  - Chlorophyll bleaching by UV-irradiation has been studied by absorbance and fluorescence spectroscopy in extracts containing mixtures of photosynthetic pigments, in acetone and n-hexane solutions, and in aqueous thylakoid suspensions. Chlorophyll undergoes destruction (bleaching) accompanied by fluorescent transient formation obeying first-order kinetics. The bleaching is governed by UV-photon energy input, as well as by different chlorophyll molecular organizations in solvents of different polarities (in vitro), and in thylakoids (in situ). UV-C-induced bleaching of chlorophylls in thylakoids is probably caused by different mechanisms compared to UV-A- and UV-B-induced bleaching.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Radiation Physics and Chemistry
T1  - Chlorophyll bleaching by UV-irradiation in vitro and in situ: Absorption and fluorescence studies
EP  - 32
IS  - 1
SP  - 25
VL  - 78
DO  - 10.1016/j.radphyschem.2008.07.006
ER  - 

@article{
author = "Zvezdanović, Jelena and Cvetic, Tijana and Veljović-Jovanović, Sonja and Marković, Dejan",
year = "2009",
abstract = "Chlorophyll bleaching by UV-irradiation has been studied by absorbance and fluorescence spectroscopy in extracts containing mixtures of photosynthetic pigments, in acetone and n-hexane solutions, and in aqueous thylakoid suspensions. Chlorophyll undergoes destruction (bleaching) accompanied by fluorescent transient formation obeying first-order kinetics. The bleaching is governed by UV-photon energy input, as well as by different chlorophyll molecular organizations in solvents of different polarities (in vitro), and in thylakoids (in situ). UV-C-induced bleaching of chlorophylls in thylakoids is probably caused by different mechanisms compared to UV-A- and UV-B-induced bleaching.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Radiation Physics and Chemistry",
title = "Chlorophyll bleaching by UV-irradiation in vitro and in situ: Absorption and fluorescence studies",
pages = "32-25",
number = "1",
volume = "78",
doi = "10.1016/j.radphyschem.2008.07.006"
}

Zvezdanović, J., Cvetic, T., Veljović-Jovanović, S.,& Marković, D.. (2009). Chlorophyll bleaching by UV-irradiation in vitro and in situ: Absorption and fluorescence studies. in Radiation Physics and Chemistry
Pergamon-Elsevier Science Ltd, Oxford., 78(1), 25-32.
https://doi.org/10.1016/j.radphyschem.2008.07.006

Zvezdanović J, Cvetic T, Veljović-Jovanović S, Marković D. Chlorophyll bleaching by UV-irradiation in vitro and in situ: Absorption and fluorescence studies. in Radiation Physics and Chemistry. 2009;78(1):25-32.
doi:10.1016/j.radphyschem.2008.07.006 .

Zvezdanović, Jelena, Cvetic, Tijana, Veljović-Jovanović, Sonja, Marković, Dejan, "Chlorophyll bleaching by UV-irradiation in vitro and in situ: Absorption and fluorescence studies" in Radiation Physics and Chemistry, 78, no. 1 (2009):25-32,
https://doi.org/10.1016/j.radphyschem.2008.07.006 . .

TY  - JOUR
AU  - Cvetic, T.
AU  - Veljović-Jovanović, Sonja
AU  - Vučinić, Željko
PY  - 2008
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/243
AB  - Spinach leaves were used to extract isoforms of NAD-dependent malate dehydrogenase (NAD-MDH) (EC 1.1.1.37), either soluble or bound to microsomal, plasma, or chloroplast envelope membranes. All fractions were subjected to isoelectric focusing analysis, which showed that purified chloroplast envelopes contain an NAD-MDH isoform tightly bound to the membranes, since treatment with 0.5 or 1% Triton X-100 was not able to release the enzyme from the envelopes. In contrast, plasma membranes released an isoform with a pI of 3.5 following treatment with 0.5% Triton X-100. The most abundant soluble leaf isoform had a pI of 9, while the chloroplast stroma contained an isoform with a pI of 5.3. Kinetic analysis of oxaloacetate (OAA)-dependent NADH oxidation in different fractions gave different K-m values for both substrates, the envelope- and plasma membrane-bound NAD-MDH exhibiting the highest affinities for OAA. Leaf plasma membrane-bound MDH exhibited a high capacity for both reaction directions (malate oxidation and OAA reduction), while the two chloroplast isoforms (stromal and envelope-bound) preferentially reduced OAA. Our results indicate that the chloroplast envelope contains a specifically attached NAD-MDH isoform that could provide direct coupling between chloroplast and cytosol adenylate pools.
PB  - Springer Wien, Wien
T2  - Protoplasma
T1  - Characterization of NAD-dependent malate dehydrogenases from spinach leaves
EP  - 253
IS  - 3-4
SP  - 247
VL  - 232
DO  - 10.1007/s00709-007-0282-7
ER  - 

@article{
author = "Cvetic, T. and Veljović-Jovanović, Sonja and Vučinić, Željko",
year = "2008",
abstract = "Spinach leaves were used to extract isoforms of NAD-dependent malate dehydrogenase (NAD-MDH) (EC 1.1.1.37), either soluble or bound to microsomal, plasma, or chloroplast envelope membranes. All fractions were subjected to isoelectric focusing analysis, which showed that purified chloroplast envelopes contain an NAD-MDH isoform tightly bound to the membranes, since treatment with 0.5 or 1% Triton X-100 was not able to release the enzyme from the envelopes. In contrast, plasma membranes released an isoform with a pI of 3.5 following treatment with 0.5% Triton X-100. The most abundant soluble leaf isoform had a pI of 9, while the chloroplast stroma contained an isoform with a pI of 5.3. Kinetic analysis of oxaloacetate (OAA)-dependent NADH oxidation in different fractions gave different K-m values for both substrates, the envelope- and plasma membrane-bound NAD-MDH exhibiting the highest affinities for OAA. Leaf plasma membrane-bound MDH exhibited a high capacity for both reaction directions (malate oxidation and OAA reduction), while the two chloroplast isoforms (stromal and envelope-bound) preferentially reduced OAA. Our results indicate that the chloroplast envelope contains a specifically attached NAD-MDH isoform that could provide direct coupling between chloroplast and cytosol adenylate pools.",
publisher = "Springer Wien, Wien",
journal = "Protoplasma",
title = "Characterization of NAD-dependent malate dehydrogenases from spinach leaves",
pages = "253-247",
number = "3-4",
volume = "232",
doi = "10.1007/s00709-007-0282-7"
}

Cvetic, T., Veljović-Jovanović, S.,& Vučinić, Ž.. (2008). Characterization of NAD-dependent malate dehydrogenases from spinach leaves. in Protoplasma
Springer Wien, Wien., 232(3-4), 247-253.
https://doi.org/10.1007/s00709-007-0282-7

Cvetic T, Veljović-Jovanović S, Vučinić Ž. Characterization of NAD-dependent malate dehydrogenases from spinach leaves. in Protoplasma. 2008;232(3-4):247-253.
doi:10.1007/s00709-007-0282-7 .

Cvetic, T., Veljović-Jovanović, Sonja, Vučinić, Željko, "Characterization of NAD-dependent malate dehydrogenases from spinach leaves" in Protoplasma, 232, no. 3-4 (2008):247-253,
https://doi.org/10.1007/s00709-007-0282-7 . .

TY  - JOUR
AU  - Veljović-Jovanović, Sonja
AU  - Kukavica, Biljana
AU  - Cvetic, T
AU  - Mojović, Miloš
AU  - Vučinić, Željko
PY  - 2005
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/138
AB  - A comparative fluorescence and oxygen radical-sensitive spin trap EPR spectroscopic study of isolated cell walls (with proteins or deproteinated), in the presence and absence of ascorbate and H2O2 is presented. Fluorescence spectra indicate the presence of at least two fluorophores, one degraded and the other synthesized after reduction or oxidation, indicating phenol di/polymerization. DEPMPO spin trap measurements show that isolated cell walls are capable of oxygen-dependent hydroxyl radical generation in the absence of NADH or other reductants, ascorbate addition, or deproteination of the cell wall abolishing the signal due to hydroxyl radicals.
PB  - New York Acad Sciences, New York
T2  - Biophysics From Molecules to Brain: in Memory of Radoslav K. Andjus
T1  - Ascorbic acid and the oxidative processes in pea root cell wall isolates - Characterization by fluorescence and EPR spectroscopy
EP  - 504
SP  - 500
VL  - 1048
DO  - 10.1196/annals.1342.076
ER  - 

@article{
author = "Veljović-Jovanović, Sonja and Kukavica, Biljana and Cvetic, T and Mojović, Miloš and Vučinić, Željko",
year = "2005",
abstract = "A comparative fluorescence and oxygen radical-sensitive spin trap EPR spectroscopic study of isolated cell walls (with proteins or deproteinated), in the presence and absence of ascorbate and H2O2 is presented. Fluorescence spectra indicate the presence of at least two fluorophores, one degraded and the other synthesized after reduction or oxidation, indicating phenol di/polymerization. DEPMPO spin trap measurements show that isolated cell walls are capable of oxygen-dependent hydroxyl radical generation in the absence of NADH or other reductants, ascorbate addition, or deproteination of the cell wall abolishing the signal due to hydroxyl radicals.",
publisher = "New York Acad Sciences, New York",
journal = "Biophysics From Molecules to Brain: in Memory of Radoslav K. Andjus",
title = "Ascorbic acid and the oxidative processes in pea root cell wall isolates - Characterization by fluorescence and EPR spectroscopy",
pages = "504-500",
volume = "1048",
doi = "10.1196/annals.1342.076"
}

Veljović-Jovanović, S., Kukavica, B., Cvetic, T., Mojović, M.,& Vučinić, Ž.. (2005). Ascorbic acid and the oxidative processes in pea root cell wall isolates - Characterization by fluorescence and EPR spectroscopy. in Biophysics From Molecules to Brain: in Memory of Radoslav K. Andjus
New York Acad Sciences, New York., 1048, 500-504.
https://doi.org/10.1196/annals.1342.076

Veljović-Jovanović S, Kukavica B, Cvetic T, Mojović M, Vučinić Ž. Ascorbic acid and the oxidative processes in pea root cell wall isolates - Characterization by fluorescence and EPR spectroscopy. in Biophysics From Molecules to Brain: in Memory of Radoslav K. Andjus. 2005;1048:500-504.
doi:10.1196/annals.1342.076 .

Veljović-Jovanović, Sonja, Kukavica, Biljana, Cvetic, T, Mojović, Miloš, Vučinić, Željko, "Ascorbic acid and the oxidative processes in pea root cell wall isolates - Characterization by fluorescence and EPR spectroscopy" in Biophysics From Molecules to Brain: in Memory of Radoslav K. Andjus, 1048 (2005):500-504,
https://doi.org/10.1196/annals.1342.076 . .