TY  - GEN
AU  - Radotić, Ksenija
AU  - Simonović Radosavljević, Jasna
AU  - Donaldson, Lloyd
AU  - Garab, Gyozo
AU  - Dudits, D.
AU  - Steinbach, Gabor
AU  - Mitrović, Aleksandra Lj.
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1232
PB  - Springer, New York
T2  - European Biophysics Journal With Biophysics Letters
T1  - Gravitropic response in woody species: role of stem structural anisotropy
EP  - S102
SP  - S102
VL  - 48
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1232
ER  - 

@misc{
author = "Radotić, Ksenija and Simonović Radosavljević, Jasna and Donaldson, Lloyd and Garab, Gyozo and Dudits, D. and Steinbach, Gabor and Mitrović, Aleksandra Lj.",
year = "2019",
publisher = "Springer, New York",
journal = "European Biophysics Journal With Biophysics Letters",
title = "Gravitropic response in woody species: role of stem structural anisotropy",
pages = "S102-S102",
volume = "48",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1232"
}

Radotić, K., Simonović Radosavljević, J., Donaldson, L., Garab, G., Dudits, D., Steinbach, G.,& Mitrović, A. Lj.. (2019). Gravitropic response in woody species: role of stem structural anisotropy. in European Biophysics Journal With Biophysics Letters
Springer, New York., 48, S102-S102.
https://hdl.handle.net/21.15107/rcub_rimsi_1232

Radotić K, Simonović Radosavljević J, Donaldson L, Garab G, Dudits D, Steinbach G, Mitrović AL. Gravitropic response in woody species: role of stem structural anisotropy. in European Biophysics Journal With Biophysics Letters. 2019;48:S102-S102.
https://hdl.handle.net/21.15107/rcub_rimsi_1232 .

Radotić, Ksenija, Simonović Radosavljević, Jasna, Donaldson, Lloyd, Garab, Gyozo, Dudits, D., Steinbach, Gabor, Mitrović, Aleksandra Lj., "Gravitropic response in woody species: role of stem structural anisotropy" in European Biophysics Journal With Biophysics Letters, 48 (2019):S102-S102,
https://hdl.handle.net/21.15107/rcub_rimsi_1232 .

TY  - GEN
AU  - Đikanović, Daniela
AU  - Kalauzi, Aleksandar
AU  - Donaldson, Lloyd
AU  - Leblanc, Roger M.
AU  - Radotić, Ksenija
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1654
AB  - Plant cell walls represent the most abundant, renewable and biodegradable composite on Earth. Its highly complex structure consists mainly of three organic compounds: cellulose, hemicelluloses, and lignin. Cell walls have wide applications in different industries, especially for biofuels and biomaterials. Fluorescence spectroscopy is the method allowing investigation of cell wall structure thought monitoring of lignin autoflorescence and thus interactions of lignin with the other cell wall constituents. Deconvolution of fluorescence spectra reveals the number and location of spectral component peaks by calculation of the approximation of the probability density (APD) of component positions. A characteristic of complex CW fluorescence is that the emission spectrum contains multiple log–normal components originating from different fluorophores, shorter wavelengths corresponding to phenolic structures and longer wavelengths to conjugated structures in lignin. Fluorescence spectroscopy has been used for fast screening of the cell wall properties from plants of different origin (hardwood, softwood and herbaceous plant), that may be important for selection of plants for possible applications. Fluorescence spectroscopy may be applicable in the investigation of the effect of stress on the cell wall. Lignin fluorescence emission spectra, peak intensities and shifts in the positions of the long-wavelength spectral components may be indicators of changes in cell wall structure during the stress. There is an increasing application of quantum dots (QDs) in plant science, as fluorescent markers. The isolated cell wall is an appropriate object for study of the interactions with nanoparticles. The results of different physico-chemical techniques including fluorescence spectroscopy combined with spectral deconvolution, show that in the cell walls, CdSe QDs predominantly bind to cellulose, via OH groups, and to lignin, via the conjugated C=C/C–C chains. Variability of bond types in lignin is related to the involvement of this polymer in plant response to various types of stress, by introducing local structural modifications in the cell wall. Different lignin model compounds have been used in order to reveal spectroscopic properties of lignin. Lignin model polymers were synthesized from three monomers, coniferyl alcohol, ferulic acid and p-coumaric acid mixed in various ratios, simulating lignin synthesis in the real cell walls. Further, by using fluorescence spectroscopy and appropriate mathematical methods, it is possible to get deeper insight into the structural characteristics of the molecule. Future investigations will be based on synthetic cell walls and on variation in a portion of all three main components: cellulose, hemicelluloses and lignin, also having in mind results of fine structural modifications in lignin model compounds.
PB  - 5th International Congress on Microscopy & Spectroscopy
T2  - 5th International Congress on Microscopy & Spectroscopy
T1  - Fluorescence Spectroscopy in Structural Studies of Plant Cell Walls
SP  - 82
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1654
ER  - 

@misc{
author = "Đikanović, Daniela and Kalauzi, Aleksandar and Donaldson, Lloyd and Leblanc, Roger M. and Radotić, Ksenija",
year = "2018",
abstract = "Plant cell walls represent the most abundant, renewable and biodegradable composite on Earth. Its highly complex structure consists mainly of three organic compounds: cellulose, hemicelluloses, and lignin. Cell walls have wide applications in different industries, especially for biofuels and biomaterials. Fluorescence spectroscopy is the method allowing investigation of cell wall structure thought monitoring of lignin autoflorescence and thus interactions of lignin with the other cell wall constituents. Deconvolution of fluorescence spectra reveals the number and location of spectral component peaks by calculation of the approximation of the probability density (APD) of component positions. A characteristic of complex CW fluorescence is that the emission spectrum contains multiple log–normal components originating from different fluorophores, shorter wavelengths corresponding to phenolic structures and longer wavelengths to conjugated structures in lignin. Fluorescence spectroscopy has been used for fast screening of the cell wall properties from plants of different origin (hardwood, softwood and herbaceous plant), that may be important for selection of plants for possible applications. Fluorescence spectroscopy may be applicable in the investigation of the effect of stress on the cell wall. Lignin fluorescence emission spectra, peak intensities and shifts in the positions of the long-wavelength spectral components may be indicators of changes in cell wall structure during the stress. There is an increasing application of quantum dots (QDs) in plant science, as fluorescent markers. The isolated cell wall is an appropriate object for study of the interactions with nanoparticles. The results of different physico-chemical techniques including fluorescence spectroscopy combined with spectral deconvolution, show that in the cell walls, CdSe QDs predominantly bind to cellulose, via OH groups, and to lignin, via the conjugated C=C/C–C chains. Variability of bond types in lignin is related to the involvement of this polymer in plant response to various types of stress, by introducing local structural modifications in the cell wall. Different lignin model compounds have been used in order to reveal spectroscopic properties of lignin. Lignin model polymers were synthesized from three monomers, coniferyl alcohol, ferulic acid and p-coumaric acid mixed in various ratios, simulating lignin synthesis in the real cell walls. Further, by using fluorescence spectroscopy and appropriate mathematical methods, it is possible to get deeper insight into the structural characteristics of the molecule. Future investigations will be based on synthetic cell walls and on variation in a portion of all three main components: cellulose, hemicelluloses and lignin, also having in mind results of fine structural modifications in lignin model compounds.",
publisher = "5th International Congress on Microscopy & Spectroscopy",
journal = "5th International Congress on Microscopy & Spectroscopy",
title = "Fluorescence Spectroscopy in Structural Studies of Plant Cell Walls",
pages = "82",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1654"
}

Đikanović, D., Kalauzi, A., Donaldson, L., Leblanc, R. M.,& Radotić, K.. (2018). Fluorescence Spectroscopy in Structural Studies of Plant Cell Walls. in 5th International Congress on Microscopy & Spectroscopy
5th International Congress on Microscopy & Spectroscopy., 82.
https://hdl.handle.net/21.15107/rcub_rimsi_1654

Đikanović D, Kalauzi A, Donaldson L, Leblanc RM, Radotić K. Fluorescence Spectroscopy in Structural Studies of Plant Cell Walls. in 5th International Congress on Microscopy & Spectroscopy. 2018;:82.
https://hdl.handle.net/21.15107/rcub_rimsi_1654 .

Đikanović, Daniela, Kalauzi, Aleksandar, Donaldson, Lloyd, Leblanc, Roger M., Radotić, Ksenija, "Fluorescence Spectroscopy in Structural Studies of Plant Cell Walls" in 5th International Congress on Microscopy & Spectroscopy (2018):82,
https://hdl.handle.net/21.15107/rcub_rimsi_1654 .

TY  - JOUR
AU  - Savić, Aleksandar G
AU  - Mitrović, Aleksandra Lj.
AU  - Donaldson, Lloyd
AU  - Simonović Radosavljević, Jasna
AU  - Bogdanović Pristov, Jelena
AU  - Steinbach, Gabor
AU  - Garab, Gyozo
AU  - Radotić, Ksenija
PY  - 2016
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/989
AB  - Fluorescence-detected linear dichroism (FDLD) microscopy provides observation of structural order in a microscopic sample and its expression in numerical terms, enabling both quantitative and qualitative comparison among different samples. We applied FDLD microscopy to compare the distribution and alignment of cellulose fibrils in cell walls of compression wood (CW) and normal wood (NW) on stem cross-sections of juvenile Picea omorika trees. Our data indicate a decrease in cellulose fibril order in CW compared with NW. Radial and tangential walls differ considerably in both NW and CW. In radial walls, cellulose fibril order shows a gradual decrease from NW to severe CW, in line with the increase in CW severity. This indicates that FDLD analysis of cellulose fibril order in radial cell walls is a valuable method for estimation of CW severity.
PB  - Cambridge Univ Press, New York
T2  - Microscopy and Microanalysis
T1  - Fluorescence-Detected Linear Dichroism of Wood Cell Walls in Juvenile Serbian Spruce: Estimation of Compression Wood Severity
EP  - 367
IS  - 2
SP  - 361
VL  - 22
DO  - 10.1017/S143192761600009X
ER  - 

@article{
author = "Savić, Aleksandar G and Mitrović, Aleksandra Lj. and Donaldson, Lloyd and Simonović Radosavljević, Jasna and Bogdanović Pristov, Jelena and Steinbach, Gabor and Garab, Gyozo and Radotić, Ksenija",
year = "2016",
abstract = "Fluorescence-detected linear dichroism (FDLD) microscopy provides observation of structural order in a microscopic sample and its expression in numerical terms, enabling both quantitative and qualitative comparison among different samples. We applied FDLD microscopy to compare the distribution and alignment of cellulose fibrils in cell walls of compression wood (CW) and normal wood (NW) on stem cross-sections of juvenile Picea omorika trees. Our data indicate a decrease in cellulose fibril order in CW compared with NW. Radial and tangential walls differ considerably in both NW and CW. In radial walls, cellulose fibril order shows a gradual decrease from NW to severe CW, in line with the increase in CW severity. This indicates that FDLD analysis of cellulose fibril order in radial cell walls is a valuable method for estimation of CW severity.",
publisher = "Cambridge Univ Press, New York",
journal = "Microscopy and Microanalysis",
title = "Fluorescence-Detected Linear Dichroism of Wood Cell Walls in Juvenile Serbian Spruce: Estimation of Compression Wood Severity",
pages = "367-361",
number = "2",
volume = "22",
doi = "10.1017/S143192761600009X"
}

Savić, A. G., Mitrović, A. Lj., Donaldson, L., Simonović Radosavljević, J., Bogdanović Pristov, J., Steinbach, G., Garab, G.,& Radotić, K.. (2016). Fluorescence-Detected Linear Dichroism of Wood Cell Walls in Juvenile Serbian Spruce: Estimation of Compression Wood Severity. in Microscopy and Microanalysis
Cambridge Univ Press, New York., 22(2), 361-367.
https://doi.org/10.1017/S143192761600009X

Savić AG, Mitrović AL, Donaldson L, Simonović Radosavljević J, Bogdanović Pristov J, Steinbach G, Garab G, Radotić K. Fluorescence-Detected Linear Dichroism of Wood Cell Walls in Juvenile Serbian Spruce: Estimation of Compression Wood Severity. in Microscopy and Microanalysis. 2016;22(2):361-367.
doi:10.1017/S143192761600009X .

Savić, Aleksandar G, Mitrović, Aleksandra Lj., Donaldson, Lloyd, Simonović Radosavljević, Jasna, Bogdanović Pristov, Jelena, Steinbach, Gabor, Garab, Gyozo, Radotić, Ksenija, "Fluorescence-Detected Linear Dichroism of Wood Cell Walls in Juvenile Serbian Spruce: Estimation of Compression Wood Severity" in Microscopy and Microanalysis, 22, no. 2 (2016):361-367,
https://doi.org/10.1017/S143192761600009X . .

TY  - JOUR
AU  - Mitrović, Aleksandra Lj.
AU  - Donaldson, Lloyd
AU  - Đikanović, Daniela
AU  - Bogdanović Pristov, Jelena
AU  - Simonović Radosavljević, Jasna
AU  - Mutavdžić, Dragosav
AU  - Kalauzi, Aleksandar
AU  - Maksimović, Vuk
AU  - Nanayakkara, Bernadette
AU  - Radotić, Ksenija
PY  - 2015
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/880
AB  - Young P. omorika trees subjected to static bending showed a severe compression wood response as characterized by fluorescence spectroscopy/microscopy, which decreased in severity with height correlated with a decrease in bending moment. This investigation is aimed at understanding the reaction wood response in a slow-growing conifer species under conditions of severe and long-term bending stress. Compression wood (CW) formation was studied in stems of juvenile P. omorika after trees were subjected to static bending by wiring at an angle of about 90 degrees, for 1 year. The applied static bending would correspond to the impact of winter snow loads or snow falls on juvenile conifers. Stem sections were collected during one growing season and examined by fluorescence microscopy, and fluorescence spectroscopy including deconvolution analysis. Trees exposed to bending produced large amounts of severe CW but very low amounts of opposite wood (OW) during the experimental season indicating a dramatic change in biomass distribution compared to control trees. Indicators of cell wall structure changes, such as fluorescence emission spectra, peak intensities, and shifts in the positions of the long-wavelength spectral components, decreased from the stem base to the top of the stem, in line with a calculated decrease in bending moment.
PB  - Springer Heidelberg, Heidelberg
T2  - Trees-Structure and Function
T1  - Analysis of static bending-induced compression wood formation in juvenile Picea omorika (Pancic) Purkyne
EP  - 1543
IS  - 5
SP  - 1533
VL  - 29
DO  - 10.1007/s00468-015-1234-z
ER  - 

@article{
author = "Mitrović, Aleksandra Lj. and Donaldson, Lloyd and Đikanović, Daniela and Bogdanović Pristov, Jelena and Simonović Radosavljević, Jasna and Mutavdžić, Dragosav and Kalauzi, Aleksandar and Maksimović, Vuk and Nanayakkara, Bernadette and Radotić, Ksenija",
year = "2015",
abstract = "Young P. omorika trees subjected to static bending showed a severe compression wood response as characterized by fluorescence spectroscopy/microscopy, which decreased in severity with height correlated with a decrease in bending moment. This investigation is aimed at understanding the reaction wood response in a slow-growing conifer species under conditions of severe and long-term bending stress. Compression wood (CW) formation was studied in stems of juvenile P. omorika after trees were subjected to static bending by wiring at an angle of about 90 degrees, for 1 year. The applied static bending would correspond to the impact of winter snow loads or snow falls on juvenile conifers. Stem sections were collected during one growing season and examined by fluorescence microscopy, and fluorescence spectroscopy including deconvolution analysis. Trees exposed to bending produced large amounts of severe CW but very low amounts of opposite wood (OW) during the experimental season indicating a dramatic change in biomass distribution compared to control trees. Indicators of cell wall structure changes, such as fluorescence emission spectra, peak intensities, and shifts in the positions of the long-wavelength spectral components, decreased from the stem base to the top of the stem, in line with a calculated decrease in bending moment.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Trees-Structure and Function",
title = "Analysis of static bending-induced compression wood formation in juvenile Picea omorika (Pancic) Purkyne",
pages = "1543-1533",
number = "5",
volume = "29",
doi = "10.1007/s00468-015-1234-z"
}

Mitrović, A. Lj., Donaldson, L., Đikanović, D., Bogdanović Pristov, J., Simonović Radosavljević, J., Mutavdžić, D., Kalauzi, A., Maksimović, V., Nanayakkara, B.,& Radotić, K.. (2015). Analysis of static bending-induced compression wood formation in juvenile Picea omorika (Pancic) Purkyne. in Trees-Structure and Function
Springer Heidelberg, Heidelberg., 29(5), 1533-1543.
https://doi.org/10.1007/s00468-015-1234-z

Mitrović AL, Donaldson L, Đikanović D, Bogdanović Pristov J, Simonović Radosavljević J, Mutavdžić D, Kalauzi A, Maksimović V, Nanayakkara B, Radotić K. Analysis of static bending-induced compression wood formation in juvenile Picea omorika (Pancic) Purkyne. in Trees-Structure and Function. 2015;29(5):1533-1543.
doi:10.1007/s00468-015-1234-z .

Mitrović, Aleksandra Lj., Donaldson, Lloyd, Đikanović, Daniela, Bogdanović Pristov, Jelena, Simonović Radosavljević, Jasna, Mutavdžić, Dragosav, Kalauzi, Aleksandar, Maksimović, Vuk, Nanayakkara, Bernadette, Radotić, Ksenija, "Analysis of static bending-induced compression wood formation in juvenile Picea omorika (Pancic) Purkyne" in Trees-Structure and Function, 29, no. 5 (2015):1533-1543,
https://doi.org/10.1007/s00468-015-1234-z . .

TY  - JOUR
AU  - Donaldson, Lloyd
AU  - Nanayakkara, Bernadette
AU  - Radotić, Ksenija
AU  - Đikanović, Daniela
AU  - Mitrović, Aleksandra Lj.
AU  - Bogdanović Pristov, Jelena
AU  - Simonović Radosavljević, Jasna
AU  - Kalauzi, Aleksandar
PY  - 2015
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/844
AB  - Cell wall fluorescence and immunocytochemistry demonstrate that xylem parenchyma cell walls do not show changes in structure and composition related to gravitropic response comparable to those of tracheids, even when they have lignified secondary cell walls. Tracheid cell walls in compression wood have altered composition and structure which generates the strain responsible for correction of stem lean as part of the gravitropic response of woody plants. Xylem parenchyma cell walls vary among conifer species and can be lignified secondary walls (spruce) or unlignified primary walls (pine). It can be expected that xylem parenchyma with lignified secondary cell walls might show features of compression wood comparable to those of tracheids that have a similar type of cell wall. A comparison of xylem parenchyma cell walls in normal and compression wood in species with lignified and non-lignified parenchyma cell walls provides a unique opportunity to understand the process of reaction wood formation in conifers. Using both UV/visible fluorescence microscopy of cell wall fluorophores and immunocytochemistry of galactan and mannan epitopes, we demonstrate that xylem parenchyma cell walls do not show the changes in composition and structure typical of compression wood tracheids. Adjacent cells of different types but with similar cell wall structure can undergo cell wall developmental changes related to support or defence functions independent of their neighbours. Tracheids are sensitive to gravitropic signals while xylem parenchyma cells are not.
PB  - Springer, New York
T2  - Planta
T1  - Xylem parenchyma cell walls lack a gravitropic response in conifer compression wood
EP  - 1424
IS  - 6
SP  - 1413
VL  - 242
DO  - 10.1007/s00425-015-2381-6
ER  - 

@article{
author = "Donaldson, Lloyd and Nanayakkara, Bernadette and Radotić, Ksenija and Đikanović, Daniela and Mitrović, Aleksandra Lj. and Bogdanović Pristov, Jelena and Simonović Radosavljević, Jasna and Kalauzi, Aleksandar",
year = "2015",
abstract = "Cell wall fluorescence and immunocytochemistry demonstrate that xylem parenchyma cell walls do not show changes in structure and composition related to gravitropic response comparable to those of tracheids, even when they have lignified secondary cell walls. Tracheid cell walls in compression wood have altered composition and structure which generates the strain responsible for correction of stem lean as part of the gravitropic response of woody plants. Xylem parenchyma cell walls vary among conifer species and can be lignified secondary walls (spruce) or unlignified primary walls (pine). It can be expected that xylem parenchyma with lignified secondary cell walls might show features of compression wood comparable to those of tracheids that have a similar type of cell wall. A comparison of xylem parenchyma cell walls in normal and compression wood in species with lignified and non-lignified parenchyma cell walls provides a unique opportunity to understand the process of reaction wood formation in conifers. Using both UV/visible fluorescence microscopy of cell wall fluorophores and immunocytochemistry of galactan and mannan epitopes, we demonstrate that xylem parenchyma cell walls do not show the changes in composition and structure typical of compression wood tracheids. Adjacent cells of different types but with similar cell wall structure can undergo cell wall developmental changes related to support or defence functions independent of their neighbours. Tracheids are sensitive to gravitropic signals while xylem parenchyma cells are not.",
publisher = "Springer, New York",
journal = "Planta",
title = "Xylem parenchyma cell walls lack a gravitropic response in conifer compression wood",
pages = "1424-1413",
number = "6",
volume = "242",
doi = "10.1007/s00425-015-2381-6"
}

Donaldson, L., Nanayakkara, B., Radotić, K., Đikanović, D., Mitrović, A. Lj., Bogdanović Pristov, J., Simonović Radosavljević, J.,& Kalauzi, A.. (2015). Xylem parenchyma cell walls lack a gravitropic response in conifer compression wood. in Planta
Springer, New York., 242(6), 1413-1424.
https://doi.org/10.1007/s00425-015-2381-6

Donaldson L, Nanayakkara B, Radotić K, Đikanović D, Mitrović AL, Bogdanović Pristov J, Simonović Radosavljević J, Kalauzi A. Xylem parenchyma cell walls lack a gravitropic response in conifer compression wood. in Planta. 2015;242(6):1413-1424.
doi:10.1007/s00425-015-2381-6 .

Donaldson, Lloyd, Nanayakkara, Bernadette, Radotić, Ksenija, Đikanović, Daniela, Mitrović, Aleksandra Lj., Bogdanović Pristov, Jelena, Simonović Radosavljević, Jasna, Kalauzi, Aleksandar, "Xylem parenchyma cell walls lack a gravitropic response in conifer compression wood" in Planta, 242, no. 6 (2015):1413-1424,
https://doi.org/10.1007/s00425-015-2381-6 . .

TY  - JOUR
AU  - Donaldson, Lloyd
AU  - Radotić, Ksenija
PY  - 2013
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/704
AB  - Wood cell walls fluoresce as a result of UV and visible light excitation due to the presence of lignin. Fluorescence spectroscopy has revealed characteristic spectral differences in various wood types, notably normal and compression wood. In order to extend this method of characterising cell walls we examined the fluorescence lifetime of wood cell walls using TCSPC (Time-Correlated Single Photon Counting) as a method of potentially detecting differences in lignin composition and measuring the molecular environment within cell walls. The fluorescence decay curves of both normal and compression wood from pine contain three exponential decay components with a mean lifetime of (m) = 473 ps in normal wood and 418 ps in compression wood. Lifetimes are spatially resolved to different cell wall layers or cell types where individual lifetimes are shown to have a log-normal distribution. The differences in fluorescence lifetime observed in pine compression wood compared to normal wood, are associated with known differences in cell wall composition such as increased p-hydroxyphenyl content in lignin as well as novel deposition of (1,4)-Galactan. Our results indicate increased deposition of lignin fluorophores with shorter lifetimes in the outer secondary wall of compression wood. We have demonstrated the usefulness of fluorescence lifetime imaging for characterising wood cell walls, offering some advantages over conventional fluorescence imaging/spectroscopy. For example, we have measured significant changes in fluorescence lifetime resulting from changes to lignin composition as a result of compression wood formation that complement similar changes in fluorescence intensity.
PB  - Wiley-Blackwell, Hoboken
T2  - Journal of Microscopy
T1  - Fluorescence lifetime imaging of lignin autofluorescence in normal and compression wood
EP  - 187
IS  - 2
SP  - 178
VL  - 251
DO  - 10.1111/jmi.12059
ER  - 

@article{
author = "Donaldson, Lloyd and Radotić, Ksenija",
year = "2013",
abstract = "Wood cell walls fluoresce as a result of UV and visible light excitation due to the presence of lignin. Fluorescence spectroscopy has revealed characteristic spectral differences in various wood types, notably normal and compression wood. In order to extend this method of characterising cell walls we examined the fluorescence lifetime of wood cell walls using TCSPC (Time-Correlated Single Photon Counting) as a method of potentially detecting differences in lignin composition and measuring the molecular environment within cell walls. The fluorescence decay curves of both normal and compression wood from pine contain three exponential decay components with a mean lifetime of (m) = 473 ps in normal wood and 418 ps in compression wood. Lifetimes are spatially resolved to different cell wall layers or cell types where individual lifetimes are shown to have a log-normal distribution. The differences in fluorescence lifetime observed in pine compression wood compared to normal wood, are associated with known differences in cell wall composition such as increased p-hydroxyphenyl content in lignin as well as novel deposition of (1,4)-Galactan. Our results indicate increased deposition of lignin fluorophores with shorter lifetimes in the outer secondary wall of compression wood. We have demonstrated the usefulness of fluorescence lifetime imaging for characterising wood cell walls, offering some advantages over conventional fluorescence imaging/spectroscopy. For example, we have measured significant changes in fluorescence lifetime resulting from changes to lignin composition as a result of compression wood formation that complement similar changes in fluorescence intensity.",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Journal of Microscopy",
title = "Fluorescence lifetime imaging of lignin autofluorescence in normal and compression wood",
pages = "187-178",
number = "2",
volume = "251",
doi = "10.1111/jmi.12059"
}

Donaldson, L.,& Radotić, K.. (2013). Fluorescence lifetime imaging of lignin autofluorescence in normal and compression wood. in Journal of Microscopy
Wiley-Blackwell, Hoboken., 251(2), 178-187.
https://doi.org/10.1111/jmi.12059

Donaldson L, Radotić K. Fluorescence lifetime imaging of lignin autofluorescence in normal and compression wood. in Journal of Microscopy. 2013;251(2):178-187.
doi:10.1111/jmi.12059 .

Donaldson, Lloyd, Radotić, Ksenija, "Fluorescence lifetime imaging of lignin autofluorescence in normal and compression wood" in Journal of Microscopy, 251, no. 2 (2013):178-187,
https://doi.org/10.1111/jmi.12059 . .

TY  - JOUR
AU  - Donaldson, Lloyd
AU  - Radotić, Ksenija
AU  - Kalauzi, Aleksandar
AU  - Đikanović, Daniela
AU  - Jeremic, Milorad G
PY  - 2010
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/418
AB  - Confocal fluorescence microscopy was used to examine the spectral characteristics of lignin autofluorescence in secondary cell walls of normal and compression wood from Pinus radiata. Using UV excitation, fluorescence spectra of normal and compression wood sections showed significant differences, especially in the outer secondary cell wall of tracheids, with a shift in maxima from violet to blue wavelengths between normal and compression wood. A comparison of normal wood, mild and severe compression wood, showed that the wavelength shift was intermediate in the mild compression wood compared to the severe compression wood, thus offering the possibility of quantifying the severity by measuring ratios of fluorescence at violet and blue wavelengths. Fluorescence induced by blue light, rather than UV, was less well differentiated amongst wood types. Spectral deconvolution indicated the presence of a minimum of five discrete lignin fluorophores in the cell walls of both normal and compression wood tracheids. Comparison with lignin model compounds suggest that the wavelength shift may correspond in part to increased levels of p-hydroxy type lignin in the compression wood samples. The combination of confocal fluorescence imaging and related spectral deconvolution therefore offers a novel technique for characterising cell wall lignin in situ.
PB  - Academic Press Inc Elsevier Science, San Diego
T2  - Journal of Structural Biology
T1  - Quantification of compression wood severity in tracheids of Pinus radiata D. Don using confocal fluorescence imaging and spectral deconvolution
EP  - 115
IS  - 1
SP  - 106
VL  - 169
DO  - 10.1016/j.jsb.2009.09.006
ER  - 

@article{
author = "Donaldson, Lloyd and Radotić, Ksenija and Kalauzi, Aleksandar and Đikanović, Daniela and Jeremic, Milorad G",
year = "2010",
abstract = "Confocal fluorescence microscopy was used to examine the spectral characteristics of lignin autofluorescence in secondary cell walls of normal and compression wood from Pinus radiata. Using UV excitation, fluorescence spectra of normal and compression wood sections showed significant differences, especially in the outer secondary cell wall of tracheids, with a shift in maxima from violet to blue wavelengths between normal and compression wood. A comparison of normal wood, mild and severe compression wood, showed that the wavelength shift was intermediate in the mild compression wood compared to the severe compression wood, thus offering the possibility of quantifying the severity by measuring ratios of fluorescence at violet and blue wavelengths. Fluorescence induced by blue light, rather than UV, was less well differentiated amongst wood types. Spectral deconvolution indicated the presence of a minimum of five discrete lignin fluorophores in the cell walls of both normal and compression wood tracheids. Comparison with lignin model compounds suggest that the wavelength shift may correspond in part to increased levels of p-hydroxy type lignin in the compression wood samples. The combination of confocal fluorescence imaging and related spectral deconvolution therefore offers a novel technique for characterising cell wall lignin in situ.",
publisher = "Academic Press Inc Elsevier Science, San Diego",
journal = "Journal of Structural Biology",
title = "Quantification of compression wood severity in tracheids of Pinus radiata D. Don using confocal fluorescence imaging and spectral deconvolution",
pages = "115-106",
number = "1",
volume = "169",
doi = "10.1016/j.jsb.2009.09.006"
}

Donaldson, L., Radotić, K., Kalauzi, A., Đikanović, D.,& Jeremic, M. G.. (2010). Quantification of compression wood severity in tracheids of Pinus radiata D. Don using confocal fluorescence imaging and spectral deconvolution. in Journal of Structural Biology
Academic Press Inc Elsevier Science, San Diego., 169(1), 106-115.
https://doi.org/10.1016/j.jsb.2009.09.006

Donaldson L, Radotić K, Kalauzi A, Đikanović D, Jeremic MG. Quantification of compression wood severity in tracheids of Pinus radiata D. Don using confocal fluorescence imaging and spectral deconvolution. in Journal of Structural Biology. 2010;169(1):106-115.
doi:10.1016/j.jsb.2009.09.006 .

Donaldson, Lloyd, Radotić, Ksenija, Kalauzi, Aleksandar, Đikanović, Daniela, Jeremic, Milorad G, "Quantification of compression wood severity in tracheids of Pinus radiata D. Don using confocal fluorescence imaging and spectral deconvolution" in Journal of Structural Biology, 169, no. 1 (2010):106-115,
https://doi.org/10.1016/j.jsb.2009.09.006 . .