TY  - JOUR
AU  - Donaldson, Lloyd
AU  - Radotić, Ksenija
PY  - 2013
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/704
AB  - Wood cell walls fluoresce as a result of UV and visible light excitation due to the presence of lignin. Fluorescence spectroscopy has revealed characteristic spectral differences in various wood types, notably normal and compression wood. In order to extend this method of characterising cell walls we examined the fluorescence lifetime of wood cell walls using TCSPC (Time-Correlated Single Photon Counting) as a method of potentially detecting differences in lignin composition and measuring the molecular environment within cell walls. The fluorescence decay curves of both normal and compression wood from pine contain three exponential decay components with a mean lifetime of (m) = 473 ps in normal wood and 418 ps in compression wood. Lifetimes are spatially resolved to different cell wall layers or cell types where individual lifetimes are shown to have a log-normal distribution. The differences in fluorescence lifetime observed in pine compression wood compared to normal wood, are associated with known differences in cell wall composition such as increased p-hydroxyphenyl content in lignin as well as novel deposition of (1,4)-Galactan. Our results indicate increased deposition of lignin fluorophores with shorter lifetimes in the outer secondary wall of compression wood. We have demonstrated the usefulness of fluorescence lifetime imaging for characterising wood cell walls, offering some advantages over conventional fluorescence imaging/spectroscopy. For example, we have measured significant changes in fluorescence lifetime resulting from changes to lignin composition as a result of compression wood formation that complement similar changes in fluorescence intensity.
PB  - Wiley-Blackwell, Hoboken
T2  - Journal of Microscopy
T1  - Fluorescence lifetime imaging of lignin autofluorescence in normal and compression wood
EP  - 187
IS  - 2
SP  - 178
VL  - 251
DO  - 10.1111/jmi.12059
ER  - 

@article{
author = "Donaldson, Lloyd and Radotić, Ksenija",
year = "2013",
abstract = "Wood cell walls fluoresce as a result of UV and visible light excitation due to the presence of lignin. Fluorescence spectroscopy has revealed characteristic spectral differences in various wood types, notably normal and compression wood. In order to extend this method of characterising cell walls we examined the fluorescence lifetime of wood cell walls using TCSPC (Time-Correlated Single Photon Counting) as a method of potentially detecting differences in lignin composition and measuring the molecular environment within cell walls. The fluorescence decay curves of both normal and compression wood from pine contain three exponential decay components with a mean lifetime of (m) = 473 ps in normal wood and 418 ps in compression wood. Lifetimes are spatially resolved to different cell wall layers or cell types where individual lifetimes are shown to have a log-normal distribution. The differences in fluorescence lifetime observed in pine compression wood compared to normal wood, are associated with known differences in cell wall composition such as increased p-hydroxyphenyl content in lignin as well as novel deposition of (1,4)-Galactan. Our results indicate increased deposition of lignin fluorophores with shorter lifetimes in the outer secondary wall of compression wood. We have demonstrated the usefulness of fluorescence lifetime imaging for characterising wood cell walls, offering some advantages over conventional fluorescence imaging/spectroscopy. For example, we have measured significant changes in fluorescence lifetime resulting from changes to lignin composition as a result of compression wood formation that complement similar changes in fluorescence intensity.",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Journal of Microscopy",
title = "Fluorescence lifetime imaging of lignin autofluorescence in normal and compression wood",
pages = "187-178",
number = "2",
volume = "251",
doi = "10.1111/jmi.12059"
}

Donaldson, L.,& Radotić, K.. (2013). Fluorescence lifetime imaging of lignin autofluorescence in normal and compression wood. in Journal of Microscopy
Wiley-Blackwell, Hoboken., 251(2), 178-187.
https://doi.org/10.1111/jmi.12059

Donaldson L, Radotić K. Fluorescence lifetime imaging of lignin autofluorescence in normal and compression wood. in Journal of Microscopy. 2013;251(2):178-187.
doi:10.1111/jmi.12059 .

Donaldson, Lloyd, Radotić, Ksenija, "Fluorescence lifetime imaging of lignin autofluorescence in normal and compression wood" in Journal of Microscopy, 251, no. 2 (2013):178-187,
https://doi.org/10.1111/jmi.12059 . .