Evaluation of genotoxic potential throughout the upper and middle stretches of Adige river basin

2016
Authors
Kracun-Kolarević, Margareta
Kolarević, Stoimir

Jovanović, Jovana
Marković, Vanja

Illic, Marija

Simonović, Predrag

Simic, Vladica

Gačić, Zoran

Diamantini, Elena
Stella, Elisa
Petrović, Mira

Majone, Bruno

Bellin, Alberto

Paunović, Momir

Vukovic-Gacic, Branka

Article (Published version)
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In this study a comprehensive genotoxicological survey throughout the upper and middle stretches of Adige river basin is presented. The study was carried out at 7 sites located along the Adige main course and one the most significant tributaries, the Noce creek, both presenting different levels of pollution pressure. To give an insight into the nature of the genotoxic activity we employed the battery of prokaryotic and eukaryotic assays. Mutagenicity in water samples was evaluated by SOS/umuC test in Salmonella typhimurium TA1535/pSK1002. The level of DNA damage as a biomarker of exposure (comet assay) and biomarker of effect (micronucleus assay) and the level of oxidative stress as well (Fpg - modified comet assay) were studied in blood cells of Salmo cenerinus Nardo, 1847 and Salmo marmoratus Cuvier, 1829. Within the applied bioassays, comet assay showed the highest potential for discriminating the sampling sites which are under lesser extent of pressure (sampling sites 1-Barnes at B...resimo and 4-Noce downstream S. Giustina) from the sites under high pressure (sampling sites 5-Noce at Mezzolombardo and 6/7-Adige upstream and downstream municipality of Trento). Significant correlation between the standard and Fpg - modified comet assay indicated that oxidative stress could be a major contributor to observed DNA damage in collected specimens.
Keywords:
Trout Adige River / Micronucleus / Ecogenotoxicology / Comet assaySource:
Science of the Total Environment, 2016, 571, 1383-1391Publisher:
- Elsevier Science Bv, Amsterdam
Funding / projects:
- Managing the effects of multiple stressors on aquatic ecosystems under water scarcity (EU-603629)
- Ministry of Education, Science and Technological Development of the Republic of Serbia
- bilateral project Serbia and Slovenia [BI-RS/14-15-013]
DOI: 10.1016/j.scitotenv.2016.07.099
ISSN: 0048-9697
PubMed: 27450952
WoS: 000383930400138
Scopus: 2-s2.0-84979519348
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Institution/Community
Institut za multidisciplinarna istraživanjaTY - JOUR AU - Kracun-Kolarević, Margareta AU - Kolarević, Stoimir AU - Jovanović, Jovana AU - Marković, Vanja AU - Illic, Marija AU - Simonović, Predrag AU - Simic, Vladica AU - Gačić, Zoran AU - Diamantini, Elena AU - Stella, Elisa AU - Petrović, Mira AU - Majone, Bruno AU - Bellin, Alberto AU - Paunović, Momir AU - Vukovic-Gacic, Branka PY - 2016 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/977 AB - In this study a comprehensive genotoxicological survey throughout the upper and middle stretches of Adige river basin is presented. The study was carried out at 7 sites located along the Adige main course and one the most significant tributaries, the Noce creek, both presenting different levels of pollution pressure. To give an insight into the nature of the genotoxic activity we employed the battery of prokaryotic and eukaryotic assays. Mutagenicity in water samples was evaluated by SOS/umuC test in Salmonella typhimurium TA1535/pSK1002. The level of DNA damage as a biomarker of exposure (comet assay) and biomarker of effect (micronucleus assay) and the level of oxidative stress as well (Fpg - modified comet assay) were studied in blood cells of Salmo cenerinus Nardo, 1847 and Salmo marmoratus Cuvier, 1829. Within the applied bioassays, comet assay showed the highest potential for discriminating the sampling sites which are under lesser extent of pressure (sampling sites 1-Barnes at Bresimo and 4-Noce downstream S. Giustina) from the sites under high pressure (sampling sites 5-Noce at Mezzolombardo and 6/7-Adige upstream and downstream municipality of Trento). Significant correlation between the standard and Fpg - modified comet assay indicated that oxidative stress could be a major contributor to observed DNA damage in collected specimens. PB - Elsevier Science Bv, Amsterdam T2 - Science of the Total Environment T1 - Evaluation of genotoxic potential throughout the upper and middle stretches of Adige river basin EP - 1391 SP - 1383 VL - 571 DO - 10.1016/j.scitotenv.2016.07.099 ER -
@article{ author = "Kracun-Kolarević, Margareta and Kolarević, Stoimir and Jovanović, Jovana and Marković, Vanja and Illic, Marija and Simonović, Predrag and Simic, Vladica and Gačić, Zoran and Diamantini, Elena and Stella, Elisa and Petrović, Mira and Majone, Bruno and Bellin, Alberto and Paunović, Momir and Vukovic-Gacic, Branka", year = "2016", abstract = "In this study a comprehensive genotoxicological survey throughout the upper and middle stretches of Adige river basin is presented. The study was carried out at 7 sites located along the Adige main course and one the most significant tributaries, the Noce creek, both presenting different levels of pollution pressure. To give an insight into the nature of the genotoxic activity we employed the battery of prokaryotic and eukaryotic assays. Mutagenicity in water samples was evaluated by SOS/umuC test in Salmonella typhimurium TA1535/pSK1002. The level of DNA damage as a biomarker of exposure (comet assay) and biomarker of effect (micronucleus assay) and the level of oxidative stress as well (Fpg - modified comet assay) were studied in blood cells of Salmo cenerinus Nardo, 1847 and Salmo marmoratus Cuvier, 1829. Within the applied bioassays, comet assay showed the highest potential for discriminating the sampling sites which are under lesser extent of pressure (sampling sites 1-Barnes at Bresimo and 4-Noce downstream S. Giustina) from the sites under high pressure (sampling sites 5-Noce at Mezzolombardo and 6/7-Adige upstream and downstream municipality of Trento). Significant correlation between the standard and Fpg - modified comet assay indicated that oxidative stress could be a major contributor to observed DNA damage in collected specimens.", publisher = "Elsevier Science Bv, Amsterdam", journal = "Science of the Total Environment", title = "Evaluation of genotoxic potential throughout the upper and middle stretches of Adige river basin", pages = "1391-1383", volume = "571", doi = "10.1016/j.scitotenv.2016.07.099" }
Kracun-Kolarević, M., Kolarević, S., Jovanović, J., Marković, V., Illic, M., Simonović, P., Simic, V., Gačić, Z., Diamantini, E., Stella, E., Petrović, M., Majone, B., Bellin, A., Paunović, M.,& Vukovic-Gacic, B.. (2016). Evaluation of genotoxic potential throughout the upper and middle stretches of Adige river basin. in Science of the Total Environment Elsevier Science Bv, Amsterdam., 571, 1383-1391. https://doi.org/10.1016/j.scitotenv.2016.07.099
Kracun-Kolarević M, Kolarević S, Jovanović J, Marković V, Illic M, Simonović P, Simic V, Gačić Z, Diamantini E, Stella E, Petrović M, Majone B, Bellin A, Paunović M, Vukovic-Gacic B. Evaluation of genotoxic potential throughout the upper and middle stretches of Adige river basin. in Science of the Total Environment. 2016;571:1383-1391. doi:10.1016/j.scitotenv.2016.07.099 .
Kracun-Kolarević, Margareta, Kolarević, Stoimir, Jovanović, Jovana, Marković, Vanja, Illic, Marija, Simonović, Predrag, Simic, Vladica, Gačić, Zoran, Diamantini, Elena, Stella, Elisa, Petrović, Mira, Majone, Bruno, Bellin, Alberto, Paunović, Momir, Vukovic-Gacic, Branka, "Evaluation of genotoxic potential throughout the upper and middle stretches of Adige river basin" in Science of the Total Environment, 571 (2016):1383-1391, https://doi.org/10.1016/j.scitotenv.2016.07.099 . .