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Rutin, a flavonoid with antioxidant activity, improves plant salinity tolerance by regulating K+ retention and Na+ exclusion from leaf mesophyll in quinoa and broad beans

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Authors
Ismail, Hebatollah
Dragišić Maksimović, Jelena
Maksimović, Vuk
Shabala, Lana
Živanović, Branka D.
Tian, Yu
Jacobsen, Sven-Erik
Shabala, Sergey
Article (Published version)
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Abstract
The causal relationship between salinity and oxidative stress tolerance is well established, but specific downstream targets and the role of specific antioxidant compounds in controlling cellular ionic homeostasis remains elusive. In this work, we have compared antioxidant profiles of leaves of two quinoa genotypes contrasting in their salt tolerance, with the aim of understanding the role of enzymatic and non-enzymatic antioxidants in salinity stress tolerance. Only changes in superoxide dismutase activity were correlated with plant adaptive responses to salinity. Proline accumulation played no major role in either osmotic adjustment or in the tissue tolerance mechanism. Among other nonenzymatic antioxidants, rutin levels were increased by over 25 fold in quinoa leaves. Exogenous application of rutin to glycophyte bean leaves improved tissue tolerance and reduced detrimental effects of salinity on leaf photochemistry. Electrophysiological experiments revealed that these beneficial eff...ects were attributed to improved potassium retention and increased rate of Na+ pumping from the cell. The lack of correlation between rutin-induced changes in K+ and H+ fluxes suggest that rutin accumulation in the cytosol scavenges hydroxyl radical formed in response to salinity treatment thus preventing K+ leak via one of ROS-activated K+ efflux pathways, rather than controlling K+ flux via voltage-gated K+-permeable channels.

Keywords:
superoxide dismutase / ROS / reactive oxygen species / proline / phenols / oxidative stress / enzymatic and non-enzymatic antioxidants / Chenopodium quinoa / catalase / ascorbate peroxidase
Source:
Functional Plant Biology, 2016, 43, 1, 75-86
Publisher:
  • Csiro Publishing, Clayton
Funding / projects:
  • Australian Research CouncilAustralian Research Council
  • Grain research and Development CorporationGrains R&D Corp
  • The membranes as sites of interaction between the intracellular and apoplastic environments: studies of the bioenergetics and signaling using biophysical and biochemical techniques. (RS-173040)
  • Egyptian Ministry of higher EducationScience and Technology Development Fund (STDF)

DOI: 10.1071/FP15312

ISSN: 1445-4408

PubMed: 32480443

WoS: 000366367900006

Scopus: 2-s2.0-84983094010
[ Google Scholar ]
57
24
URI
http://rimsi.imsi.bg.ac.rs/handle/123456789/971
Collections
  • Radovi istraživača / Researchers’ publications
Institution/Community
Institut za multidisciplinarna istraživanja
TY  - JOUR
AU  - Ismail, Hebatollah
AU  - Dragišić Maksimović, Jelena
AU  - Maksimović, Vuk
AU  - Shabala, Lana
AU  - Živanović, Branka  D.
AU  - Tian, Yu
AU  - Jacobsen, Sven-Erik
AU  - Shabala, Sergey
PY  - 2016
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/971
AB  - The causal relationship between salinity and oxidative stress tolerance is well established, but specific downstream targets and the role of specific antioxidant compounds in controlling cellular ionic homeostasis remains elusive. In this work, we have compared antioxidant profiles of leaves of two quinoa genotypes contrasting in their salt tolerance, with the aim of understanding the role of enzymatic and non-enzymatic antioxidants in salinity stress tolerance. Only changes in superoxide dismutase activity were correlated with plant adaptive responses to salinity. Proline accumulation played no major role in either osmotic adjustment or in the tissue tolerance mechanism. Among other nonenzymatic antioxidants, rutin levels were increased by over 25 fold in quinoa leaves. Exogenous application of rutin to glycophyte bean leaves improved tissue tolerance and reduced detrimental effects of salinity on leaf photochemistry. Electrophysiological experiments revealed that these beneficial effects were attributed to improved potassium retention and increased rate of Na+ pumping from the cell. The lack of correlation between rutin-induced changes in K+ and H+ fluxes suggest that rutin accumulation in the cytosol scavenges hydroxyl radical formed in response to salinity treatment thus preventing K+ leak via one of ROS-activated K+ efflux pathways, rather than controlling K+ flux via voltage-gated K+-permeable channels.
PB  - Csiro Publishing, Clayton
T2  - Functional Plant Biology
T1  - Rutin, a flavonoid with antioxidant activity, improves plant salinity tolerance by regulating K+ retention and Na+ exclusion from leaf mesophyll in quinoa and broad beans
EP  - 86
IS  - 1
SP  - 75
VL  - 43
DO  - 10.1071/FP15312
ER  - 
@article{
author = "Ismail, Hebatollah and Dragišić Maksimović, Jelena and Maksimović, Vuk and Shabala, Lana and Živanović, Branka  D. and Tian, Yu and Jacobsen, Sven-Erik and Shabala, Sergey",
year = "2016",
abstract = "The causal relationship between salinity and oxidative stress tolerance is well established, but specific downstream targets and the role of specific antioxidant compounds in controlling cellular ionic homeostasis remains elusive. In this work, we have compared antioxidant profiles of leaves of two quinoa genotypes contrasting in their salt tolerance, with the aim of understanding the role of enzymatic and non-enzymatic antioxidants in salinity stress tolerance. Only changes in superoxide dismutase activity were correlated with plant adaptive responses to salinity. Proline accumulation played no major role in either osmotic adjustment or in the tissue tolerance mechanism. Among other nonenzymatic antioxidants, rutin levels were increased by over 25 fold in quinoa leaves. Exogenous application of rutin to glycophyte bean leaves improved tissue tolerance and reduced detrimental effects of salinity on leaf photochemistry. Electrophysiological experiments revealed that these beneficial effects were attributed to improved potassium retention and increased rate of Na+ pumping from the cell. The lack of correlation between rutin-induced changes in K+ and H+ fluxes suggest that rutin accumulation in the cytosol scavenges hydroxyl radical formed in response to salinity treatment thus preventing K+ leak via one of ROS-activated K+ efflux pathways, rather than controlling K+ flux via voltage-gated K+-permeable channels.",
publisher = "Csiro Publishing, Clayton",
journal = "Functional Plant Biology",
title = "Rutin, a flavonoid with antioxidant activity, improves plant salinity tolerance by regulating K+ retention and Na+ exclusion from leaf mesophyll in quinoa and broad beans",
pages = "86-75",
number = "1",
volume = "43",
doi = "10.1071/FP15312"
}
Ismail, H., Dragišić Maksimović, J., Maksimović, V., Shabala, L., Živanović, Branka  D., Tian, Y., Jacobsen, S.,& Shabala, S.. (2016). Rutin, a flavonoid with antioxidant activity, improves plant salinity tolerance by regulating K+ retention and Na+ exclusion from leaf mesophyll in quinoa and broad beans. in Functional Plant Biology
Csiro Publishing, Clayton., 43(1), 75-86.
https://doi.org/10.1071/FP15312
Ismail H, Dragišić Maksimović J, Maksimović V, Shabala L, Živanović, Branka  D., Tian Y, Jacobsen S, Shabala S. Rutin, a flavonoid with antioxidant activity, improves plant salinity tolerance by regulating K+ retention and Na+ exclusion from leaf mesophyll in quinoa and broad beans. in Functional Plant Biology. 2016;43(1):75-86.
doi:10.1071/FP15312 .
Ismail, Hebatollah, Dragišić Maksimović, Jelena, Maksimović, Vuk, Shabala, Lana, Živanović, Branka  D., Tian, Yu, Jacobsen, Sven-Erik, Shabala, Sergey, "Rutin, a flavonoid with antioxidant activity, improves plant salinity tolerance by regulating K+ retention and Na+ exclusion from leaf mesophyll in quinoa and broad beans" in Functional Plant Biology, 43, no. 1 (2016):75-86,
https://doi.org/10.1071/FP15312 . .

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