Physiological basis of differential zinc and copper tolerance of Verbascum populations from metal-contaminated and uncontaminated areas
Само за регистроване кориснике
2016
Чланак у часопису (Објављена верзија)

Метаподаци
Приказ свих података о документуАпстракт
Metal contamination represents a strong selective pressure favoring tolerant genotypes and leading to differentiation between plant populations. We investigated the adaptive capacity of early-colonizer species of Verbascum recently exposed to Zn- and Cu-contaminated soils (10-20 years). Two Verbascum thapsus L. populations from uncontaminated sites (NMET1, NMET2), one V. thapsus from a zinc-contaminated site (MET1), and a Verbascum lychnitis population from an open-cast copper mine (MET2) were exposed to elevated Zn or Cu in hydroponic culture under glasshouse conditions. MET populations showed considerably higher tolerance to both Zn and Cu than NMET populations as assessed by measurements of growth and net photosynthesis, yet they accumulated higher tissue Zn concentrations in the shoot. Abscisic acid (ABA) concentration increased with Zn and Cu treatment in the NMET populations, which was correlated to stomatal closure, decrease of net photosynthesis, and nutritional imbalance, indi...cative of interference with xylem loading and divalent-cation homeostasis. At the cellular level, the sensitivity of NMET2 to Zn and Cu was reflected in significant metal-induced ROS accumulation and ion leakage from roots as well as strong induction of peroxidase activity (POD, EC 1.11.1.7), while Zn had no significant effect on ABA concentration and POD activity in MET1. Interestingly, MET2 had constitutively higher root ABA concentration and POD activity. We propose that ABA distribution between shoots and roots could represent an adaptive mechanism for maintaining low ABA levels and unaffected stomatal conductance. The results show that metal tolerance can occur in Verbascum populations after relatively short time of exposure to metal-contaminated soil, indicating their potential use for phytostabilization.
Кључне речи:
Zinc / Verbascum thapsus / Verbascum lychnitis / Metal tolerance / Hydrogen peroxide / Copper / Abscisic acidИзвор:
Environmental Science and Pollution Research, 2016, 23, 10, 10005-10020Издавач:
- Springer Heidelberg, Heidelberg
Финансирање / пројекти:
- Модификације антиоксидативног метаболизма биљака са циљем повећања толеранције на абиотски стрес и идентификација нових биомаркера са применом у ремедијацији и мониторингу деградираних станишта (RS-43010)
- OSI/FCO scholarship
DOI: 10.1007/s11356-016-6177-4
ISSN: 0944-1344
PubMed: 26865485
WoS: 000376421400069
Scopus: 2-s2.0-84957611641
Институција/група
Institut za multidisciplinarna istraživanjaTY - JOUR AU - Morina, Filis AU - Jovanović, Ljubinko AU - Prokic, Ljiljana AU - Veljović-Jovanović, Sonja PY - 2016 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/934 AB - Metal contamination represents a strong selective pressure favoring tolerant genotypes and leading to differentiation between plant populations. We investigated the adaptive capacity of early-colonizer species of Verbascum recently exposed to Zn- and Cu-contaminated soils (10-20 years). Two Verbascum thapsus L. populations from uncontaminated sites (NMET1, NMET2), one V. thapsus from a zinc-contaminated site (MET1), and a Verbascum lychnitis population from an open-cast copper mine (MET2) were exposed to elevated Zn or Cu in hydroponic culture under glasshouse conditions. MET populations showed considerably higher tolerance to both Zn and Cu than NMET populations as assessed by measurements of growth and net photosynthesis, yet they accumulated higher tissue Zn concentrations in the shoot. Abscisic acid (ABA) concentration increased with Zn and Cu treatment in the NMET populations, which was correlated to stomatal closure, decrease of net photosynthesis, and nutritional imbalance, indicative of interference with xylem loading and divalent-cation homeostasis. At the cellular level, the sensitivity of NMET2 to Zn and Cu was reflected in significant metal-induced ROS accumulation and ion leakage from roots as well as strong induction of peroxidase activity (POD, EC 1.11.1.7), while Zn had no significant effect on ABA concentration and POD activity in MET1. Interestingly, MET2 had constitutively higher root ABA concentration and POD activity. We propose that ABA distribution between shoots and roots could represent an adaptive mechanism for maintaining low ABA levels and unaffected stomatal conductance. The results show that metal tolerance can occur in Verbascum populations after relatively short time of exposure to metal-contaminated soil, indicating their potential use for phytostabilization. PB - Springer Heidelberg, Heidelberg T2 - Environmental Science and Pollution Research T1 - Physiological basis of differential zinc and copper tolerance of Verbascum populations from metal-contaminated and uncontaminated areas EP - 10020 IS - 10 SP - 10005 VL - 23 DO - 10.1007/s11356-016-6177-4 ER -
@article{ author = "Morina, Filis and Jovanović, Ljubinko and Prokic, Ljiljana and Veljović-Jovanović, Sonja", year = "2016", abstract = "Metal contamination represents a strong selective pressure favoring tolerant genotypes and leading to differentiation between plant populations. We investigated the adaptive capacity of early-colonizer species of Verbascum recently exposed to Zn- and Cu-contaminated soils (10-20 years). Two Verbascum thapsus L. populations from uncontaminated sites (NMET1, NMET2), one V. thapsus from a zinc-contaminated site (MET1), and a Verbascum lychnitis population from an open-cast copper mine (MET2) were exposed to elevated Zn or Cu in hydroponic culture under glasshouse conditions. MET populations showed considerably higher tolerance to both Zn and Cu than NMET populations as assessed by measurements of growth and net photosynthesis, yet they accumulated higher tissue Zn concentrations in the shoot. Abscisic acid (ABA) concentration increased with Zn and Cu treatment in the NMET populations, which was correlated to stomatal closure, decrease of net photosynthesis, and nutritional imbalance, indicative of interference with xylem loading and divalent-cation homeostasis. At the cellular level, the sensitivity of NMET2 to Zn and Cu was reflected in significant metal-induced ROS accumulation and ion leakage from roots as well as strong induction of peroxidase activity (POD, EC 1.11.1.7), while Zn had no significant effect on ABA concentration and POD activity in MET1. Interestingly, MET2 had constitutively higher root ABA concentration and POD activity. We propose that ABA distribution between shoots and roots could represent an adaptive mechanism for maintaining low ABA levels and unaffected stomatal conductance. The results show that metal tolerance can occur in Verbascum populations after relatively short time of exposure to metal-contaminated soil, indicating their potential use for phytostabilization.", publisher = "Springer Heidelberg, Heidelberg", journal = "Environmental Science and Pollution Research", title = "Physiological basis of differential zinc and copper tolerance of Verbascum populations from metal-contaminated and uncontaminated areas", pages = "10020-10005", number = "10", volume = "23", doi = "10.1007/s11356-016-6177-4" }
Morina, F., Jovanović, L., Prokic, L.,& Veljović-Jovanović, S.. (2016). Physiological basis of differential zinc and copper tolerance of Verbascum populations from metal-contaminated and uncontaminated areas. in Environmental Science and Pollution Research Springer Heidelberg, Heidelberg., 23(10), 10005-10020. https://doi.org/10.1007/s11356-016-6177-4
Morina F, Jovanović L, Prokic L, Veljović-Jovanović S. Physiological basis of differential zinc and copper tolerance of Verbascum populations from metal-contaminated and uncontaminated areas. in Environmental Science and Pollution Research. 2016;23(10):10005-10020. doi:10.1007/s11356-016-6177-4 .
Morina, Filis, Jovanović, Ljubinko, Prokic, Ljiljana, Veljović-Jovanović, Sonja, "Physiological basis of differential zinc and copper tolerance of Verbascum populations from metal-contaminated and uncontaminated areas" in Environmental Science and Pollution Research, 23, no. 10 (2016):10005-10020, https://doi.org/10.1007/s11356-016-6177-4 . .