RIMSI - Repository of Institute for Multidisciplinary Research
University of Belgrade - Institute for Multidisciplinary Research
    • English
    • Српски
    • Српски (Serbia)
  • English 
    • English
    • Serbian (Cyrillic)
    • Serbian (Latin)
  • Login
View Item 
  •   RIMSI
  • Institut za multidisciplinarna istraživanja
  • Radovi istraživača / Researchers’ publications
  • View Item
  •   RIMSI
  • Institut za multidisciplinarna istraživanja
  • Radovi istraživača / Researchers’ publications
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

The role of autophagy and lipolysis in survival of astrocytes under nutrient deprivation

Authorized Users Only
2015
Authors
Korenic, Andrej
Andjus, Pavle R.
Radenović, Lidija
Spasojević, Ivan
Article (Published version)
Metadata
Show full item record
Abstract
Astrocytes can survive nutrient deprivation (ND) for days. However, the pro-survival strategy of astrocytes under such a metabolic challenge is still not clear. In the present study, we examined the effects of inhibition of two potential steps in energy acquisition during ND: autophagy (using chloroquine) and lipolysis (using orlistat). The inhibition of autophagy did not show significant effects on cell viability until 8-9 h of ND. From that point onwards, the number of dead cells gradually increased, reaching similar to 60% between 10 and 12 h of ND. In addition, early inhibition of autophagy made astrocytes more vulnerable to the latter ND. The inhibition of lipolysis decreased the viability of cells exposed to ND, but this appeared much later compared to the inhibition of autophagy. The application of orlistat prevented ND-related hyperpolarization of the mitochondrial membrane, and mitochondria became swollen. This study clearly shows that autophagy and lipolysis are essential for... the survival of astrocytes under ND conditions, which might be related to their role as neuron-supporting cells.

Keywords:
Nutrient deprivation / Mitochondrial membrane potential / Lipolysis / Autophagy / Astrocytes
Source:
Neuroscience Letters, 2015, 595, 128-133
Publisher:
  • Elsevier Ireland Ltd, Clare
Funding / projects:
  • Biomarkers in neurodegenerative and malignant processes (RS-41005)

DOI: 10.1016/j.neulet.2015.04.020

ISSN: 0304-3940

PubMed: 25888813

WoS: 000355029800025

Scopus: 2-s2.0-84927945861
[ Google Scholar ]
9
5
URI
http://rimsi.imsi.bg.ac.rs/handle/123456789/924
Collections
  • Radovi istraživača / Researchers’ publications
Institution/Community
Institut za multidisciplinarna istraživanja
TY  - JOUR
AU  - Korenic, Andrej
AU  - Andjus, Pavle R.
AU  - Radenović, Lidija
AU  - Spasojević, Ivan
PY  - 2015
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/924
AB  - Astrocytes can survive nutrient deprivation (ND) for days. However, the pro-survival strategy of astrocytes under such a metabolic challenge is still not clear. In the present study, we examined the effects of inhibition of two potential steps in energy acquisition during ND: autophagy (using chloroquine) and lipolysis (using orlistat). The inhibition of autophagy did not show significant effects on cell viability until 8-9 h of ND. From that point onwards, the number of dead cells gradually increased, reaching similar to 60% between 10 and 12 h of ND. In addition, early inhibition of autophagy made astrocytes more vulnerable to the latter ND. The inhibition of lipolysis decreased the viability of cells exposed to ND, but this appeared much later compared to the inhibition of autophagy. The application of orlistat prevented ND-related hyperpolarization of the mitochondrial membrane, and mitochondria became swollen. This study clearly shows that autophagy and lipolysis are essential for the survival of astrocytes under ND conditions, which might be related to their role as neuron-supporting cells.
PB  - Elsevier Ireland Ltd, Clare
T2  - Neuroscience Letters
T1  - The role of autophagy and lipolysis in survival of astrocytes under nutrient deprivation
EP  - 133
SP  - 128
VL  - 595
DO  - 10.1016/j.neulet.2015.04.020
ER  - 
@article{
author = "Korenic, Andrej and Andjus, Pavle R. and Radenović, Lidija and Spasojević, Ivan",
year = "2015",
abstract = "Astrocytes can survive nutrient deprivation (ND) for days. However, the pro-survival strategy of astrocytes under such a metabolic challenge is still not clear. In the present study, we examined the effects of inhibition of two potential steps in energy acquisition during ND: autophagy (using chloroquine) and lipolysis (using orlistat). The inhibition of autophagy did not show significant effects on cell viability until 8-9 h of ND. From that point onwards, the number of dead cells gradually increased, reaching similar to 60% between 10 and 12 h of ND. In addition, early inhibition of autophagy made astrocytes more vulnerable to the latter ND. The inhibition of lipolysis decreased the viability of cells exposed to ND, but this appeared much later compared to the inhibition of autophagy. The application of orlistat prevented ND-related hyperpolarization of the mitochondrial membrane, and mitochondria became swollen. This study clearly shows that autophagy and lipolysis are essential for the survival of astrocytes under ND conditions, which might be related to their role as neuron-supporting cells.",
publisher = "Elsevier Ireland Ltd, Clare",
journal = "Neuroscience Letters",
title = "The role of autophagy and lipolysis in survival of astrocytes under nutrient deprivation",
pages = "133-128",
volume = "595",
doi = "10.1016/j.neulet.2015.04.020"
}
Korenic, A., Andjus, P. R., Radenović, L.,& Spasojević, I.. (2015). The role of autophagy and lipolysis in survival of astrocytes under nutrient deprivation. in Neuroscience Letters
Elsevier Ireland Ltd, Clare., 595, 128-133.
https://doi.org/10.1016/j.neulet.2015.04.020
Korenic A, Andjus PR, Radenović L, Spasojević I. The role of autophagy and lipolysis in survival of astrocytes under nutrient deprivation. in Neuroscience Letters. 2015;595:128-133.
doi:10.1016/j.neulet.2015.04.020 .
Korenic, Andrej, Andjus, Pavle R., Radenović, Lidija, Spasojević, Ivan, "The role of autophagy and lipolysis in survival of astrocytes under nutrient deprivation" in Neuroscience Letters, 595 (2015):128-133,
https://doi.org/10.1016/j.neulet.2015.04.020 . .

DSpace software copyright © 2002-2015  DuraSpace
About RIMSI | Send Feedback

OpenAIRERCUB
 

 

All of DSpaceCommunitiesAuthorsTitlesSubjectsThis institutionAuthorsTitlesSubjects

Statistics

View Usage Statistics

DSpace software copyright © 2002-2015  DuraSpace
About RIMSI | Send Feedback

OpenAIRERCUB