Effects of acute cooling on fish electroretinogram: A comparative study

Abstract

Temperature dependence of electroretinogram (ERG) was investigated in 3 fish species occupying different habitats - dogfish shark (Scyliorhinus canicula), Prussian carp (Carassius gibelio) and European eel (Anguilla anguilla). Acute cooling of the shark isolated eyecup from 23 degrees C down to 6 degrees C induced suppression of the electroretinographic b-wave - a complete degradation of this component was observed at 6 degrees C. On the other hand, photoreceptor component of the ERG, the negative late receptor potential was not affected by cooling. The fact that the suppression of the dogfish shark b-wave at low temperatures was as a rule irreversible testifies about breakdown of neural retinal function at cold temperature extremes. Although in vivo experiments on immobilized Prussian carps have never resulted in complete deterioration of the b-wave at low temperatures, significant suppression of this ERG component by cooling was detected. Suppressing the effect of low temperatures on Prussian carp ERG might be due to the fact that C. gibelio, as well as other cyprinids, can be characterized as a warmwater species preferring temperatures well above cold extremes. The ERG of the eel, the third examined species, exhibited the strongest resistance to extremely low temperatures. During acute cooling of in situ eyecup preparations of migrating silver eels from 30 degrees C down to 2 degrees C the form of ERG became wider, but the amplitude of the b-wave only slightly decreased. High tolerance of eel b-wave to cold extremes shown in our study complies with ecological data confirming eurythermia in migrating silver eels remarkably adapted to cold-water environment as well.