Cell Death Parameters as Revealed by Whole-Cell Patch-Clamp and Interval Weighted Spectra Averaging: Changes in Membrane Properties and Current Frequency of Cultured Mouse Microglial Cells Induced by Glutaraldehyde
Само за регистроване кориснике
2015
Чланак у часопису (Објављена верзија)

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The physiological and biochemical factors that lead to cell death have not been recognized completely. To our knowledge, there are no data on the bioelectric parameters that characterize early period of cell death, as well as on the appearance of related membrane current frequencies. We studied early parameters of glutaraldehyde (GA)-induced cell death, by examining the membrane properties of mouse microglia using the whole-cell patch-clamp technique. In addition, we investigated the GA-induced changes in the membrane current frequency, to see if characteristic frequencies would appear in dying cell. For data analysis, we applied a new approach, an improved multiple moving window length analysis and interval weighted spectra averaging (IWSA). We chose GA for its ability to induce almost instantaneous cell death. The 0.6 % GA did not induce changes in the bioelectric membrane properties of microglia. However, the 3 % GA caused significant decrease of membrane capacitance and resistance ...accompanied by the prominent increase in the membrane currents and nearly ohmic current response of microglial cells. These data indicate that 3 % GA caused complete loss of the membrane function consequently inducing instantaneous cell death. The membrane function loss was characterized by appearance of the 1.26-4.62 Hz frequency peak in the IWSA spectra, while no significant increase of amplitudes could be observed for cells treated with 0.6 % GA. To our knowledge, this is the first record of a frequency associated with complete loss of the membrane function and thus can be considered as an early indicator of cell death.
Кључне речи:
Whole-cell current / Patch-clamp / Microglia / Membrane current frequency / Interval weighted spectra averagingИзвор:
Journal of Membrane Biology, 2015, 248, 1, 117-123Издавач:
- Springer, New York
Финансирање / пројекти:
- Синтеза, процесирање и карактеризација наноструктурних материјала за примену у области енергије, механичког инжењерства, заштите животне стредине и биомедицине (RS-45012)
- Ћелијска и молекулска основа неуроинфламације: потенцијала циљна места за транслациону медицину и терапију (RS-41014)
- Неуробиологија спавања у старењу и болести - електроенцефалографски маркери и моделирање у процени поремећаја (RS-173022)
DOI: 10.1007/s00232-014-9748-7
ISSN: 0022-2631
PubMed: 25367146
WoS: 000348279300013
Scopus: 2-s2.0-84922003106
Институција/група
Institut za multidisciplinarna istraživanjaTY - JOUR AU - Kalauzi, Aleksandar AU - Nikolić, Ljiljana M. AU - Savić, Danijela Z AU - Radotić, Ksenija PY - 2015 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/897 AB - The physiological and biochemical factors that lead to cell death have not been recognized completely. To our knowledge, there are no data on the bioelectric parameters that characterize early period of cell death, as well as on the appearance of related membrane current frequencies. We studied early parameters of glutaraldehyde (GA)-induced cell death, by examining the membrane properties of mouse microglia using the whole-cell patch-clamp technique. In addition, we investigated the GA-induced changes in the membrane current frequency, to see if characteristic frequencies would appear in dying cell. For data analysis, we applied a new approach, an improved multiple moving window length analysis and interval weighted spectra averaging (IWSA). We chose GA for its ability to induce almost instantaneous cell death. The 0.6 % GA did not induce changes in the bioelectric membrane properties of microglia. However, the 3 % GA caused significant decrease of membrane capacitance and resistance accompanied by the prominent increase in the membrane currents and nearly ohmic current response of microglial cells. These data indicate that 3 % GA caused complete loss of the membrane function consequently inducing instantaneous cell death. The membrane function loss was characterized by appearance of the 1.26-4.62 Hz frequency peak in the IWSA spectra, while no significant increase of amplitudes could be observed for cells treated with 0.6 % GA. To our knowledge, this is the first record of a frequency associated with complete loss of the membrane function and thus can be considered as an early indicator of cell death. PB - Springer, New York T2 - Journal of Membrane Biology T1 - Cell Death Parameters as Revealed by Whole-Cell Patch-Clamp and Interval Weighted Spectra Averaging: Changes in Membrane Properties and Current Frequency of Cultured Mouse Microglial Cells Induced by Glutaraldehyde EP - 123 IS - 1 SP - 117 VL - 248 DO - 10.1007/s00232-014-9748-7 ER -
@article{ author = "Kalauzi, Aleksandar and Nikolić, Ljiljana M. and Savić, Danijela Z and Radotić, Ksenija", year = "2015", abstract = "The physiological and biochemical factors that lead to cell death have not been recognized completely. To our knowledge, there are no data on the bioelectric parameters that characterize early period of cell death, as well as on the appearance of related membrane current frequencies. We studied early parameters of glutaraldehyde (GA)-induced cell death, by examining the membrane properties of mouse microglia using the whole-cell patch-clamp technique. In addition, we investigated the GA-induced changes in the membrane current frequency, to see if characteristic frequencies would appear in dying cell. For data analysis, we applied a new approach, an improved multiple moving window length analysis and interval weighted spectra averaging (IWSA). We chose GA for its ability to induce almost instantaneous cell death. The 0.6 % GA did not induce changes in the bioelectric membrane properties of microglia. However, the 3 % GA caused significant decrease of membrane capacitance and resistance accompanied by the prominent increase in the membrane currents and nearly ohmic current response of microglial cells. These data indicate that 3 % GA caused complete loss of the membrane function consequently inducing instantaneous cell death. The membrane function loss was characterized by appearance of the 1.26-4.62 Hz frequency peak in the IWSA spectra, while no significant increase of amplitudes could be observed for cells treated with 0.6 % GA. To our knowledge, this is the first record of a frequency associated with complete loss of the membrane function and thus can be considered as an early indicator of cell death.", publisher = "Springer, New York", journal = "Journal of Membrane Biology", title = "Cell Death Parameters as Revealed by Whole-Cell Patch-Clamp and Interval Weighted Spectra Averaging: Changes in Membrane Properties and Current Frequency of Cultured Mouse Microglial Cells Induced by Glutaraldehyde", pages = "123-117", number = "1", volume = "248", doi = "10.1007/s00232-014-9748-7" }
Kalauzi, A., Nikolić, L. M., Savić, D. Z.,& Radotić, K.. (2015). Cell Death Parameters as Revealed by Whole-Cell Patch-Clamp and Interval Weighted Spectra Averaging: Changes in Membrane Properties and Current Frequency of Cultured Mouse Microglial Cells Induced by Glutaraldehyde. in Journal of Membrane Biology Springer, New York., 248(1), 117-123. https://doi.org/10.1007/s00232-014-9748-7
Kalauzi A, Nikolić LM, Savić DZ, Radotić K. Cell Death Parameters as Revealed by Whole-Cell Patch-Clamp and Interval Weighted Spectra Averaging: Changes in Membrane Properties and Current Frequency of Cultured Mouse Microglial Cells Induced by Glutaraldehyde. in Journal of Membrane Biology. 2015;248(1):117-123. doi:10.1007/s00232-014-9748-7 .
Kalauzi, Aleksandar, Nikolić, Ljiljana M., Savić, Danijela Z, Radotić, Ksenija, "Cell Death Parameters as Revealed by Whole-Cell Patch-Clamp and Interval Weighted Spectra Averaging: Changes in Membrane Properties and Current Frequency of Cultured Mouse Microglial Cells Induced by Glutaraldehyde" in Journal of Membrane Biology, 248, no. 1 (2015):117-123, https://doi.org/10.1007/s00232-014-9748-7 . .