Estimation of meristemoid complexity during Tacitus bellus in vitro shoot organogenesis by 2D fractal analysis

Abstract

This study considered the analysis of 2D fractal characteristics of cell populations that participate in meristemoid formation during the process of de novo shoot organogenesis in Tacitus bellus leaf explant culture. Micrographs representing early and late meristemoid formation stages were used for 2D fractal analysis. Analysis showed that meristemoid complexity at two selected points of development were characterized by significantly different fractal dimension values. This result demonstrates that the proposed method is efficient for the fine distinction of histologically similar structures at an appropriate resolution of micrographs.