Genotoxic potential and heart rate disorders in the Mediterranean mussel Mytilus galloprovincialis exposed to Superdispersant-25 and dispersed diesel oil

Abstract

The effects of ex situ exposure of Mytilus galloprovincialis to Superdispersant-25 (S-25), diesel oil and dispersed diesel oil mixtures were studied by the impact on level of DNA damage in haemocytes (comet assay) and the cardiac activity patterns of mussels. Specimens were exposed for 72 h in a static system to diesel oil (100 mu L/L and 1 mL/L), S-25 (5 and 50 mu L/L), and dispersed diesel oil mixtures M1 (diesel oil 100 g mu L/L + S-25 5 mu L/L) and M2 (diesel oil 1 mL/L + S-25 50 mu L/L). For positive control 40 mu M CdCl2 was used. The comet assay results indicated genotoxic potential of S-25 while the effects of diesel oil alone were not observed. The highest response was detected for M1 while the effects of M2 were not detected. The heart rate disorders were recorded for the diesel oil (1 mL/L), S-25 (50 mu L/L) and both dispersed diesel oil mixtures.