Приказ основних података о документу

dc.creatorTimotijević, Gordana
dc.creatorMilisavljević, Mira
dc.creatorNikolic, Dragana B.
dc.creatorMilovanović, Bosko M.
dc.creatorNikolic, Dragana S.
dc.creatorNikolic, Miroslav
dc.creatorSamardžić, Jelena T.
dc.date.accessioned2022-04-05T14:54:56Z
dc.date.available2022-04-05T14:54:56Z
dc.date.issued2015
dc.identifier.issn0534-0012
dc.identifier.urihttp://rimsi.imsi.bg.ac.rs/handle/123456789/848
dc.description.abstractMicroRNAs (miRNAs) belong to the class of small non-coding RNAs which have important roles throughout development as well as in plant response to diverse environmental stresses. Some of plant miRNAs are essential for regulation and maintenance of nutritive homeostasis when nutrients are in excess or shortage comparing to optimal concentration for certain plant species. Better understanding of miRNAs functions implies development of efficient technology for profiling their gene expression. We set out to establish validate the methodology for miRNA gene expression analysis in cucumber grown under suboptimal mineral nutrient regimes, including iron deficiency. Reverse transcription by "stem-loop" primers in combination with Real time PCR method is one of potential approaches for quantification of miRNA gene expression. In this paper we presented a method for "stem loop" primer design specific for miR398, as well as reaction optimization and determination of Real time PCR efficiency. Proving the accuracy of this method was imperative as "stem loop" RT which consider separate transcription of target and endogenous control. The method was verified by comparison of the obtained data with results of miR398 expression achieved using a commercial kit based on simultaneous conversion of all RNAs in cDNAs.en
dc.publisherDruštvo genetičara Srbije, Beograd
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173005/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173028/RS//
dc.rightsopenAccess
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.sourceGenetika-Belgrade
dc.subjectReal time PCRen
dc.subjectmiRNA gene expression analysisen
dc.subjectiron deficiencyen
dc.titleEstablishment and in-house validation of stem-loop rt pcr method for microrna398 expression analysisen
dc.typearticle
dc.rights.licenseBY-NC-ND
dc.citation.epage416
dc.citation.issue2
dc.citation.other47(2): 405-416
dc.citation.rankM23
dc.citation.spage405
dc.citation.volume47
dc.identifier.doi10.2298/GENSR1502405T
dc.identifier.fulltexthttp://rimsi.imsi.bg.ac.rs/bitstream/id/1005/845.pdf
dc.identifier.scopus2-s2.0-84945961001
dc.identifier.wos000363055800004
dc.type.versionpublishedVersion


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Приказ основних података о документу