The effects of long-term exposure (5 h) of Phycomyces blakesleeanus mycelium to 5 mM KCN on respiration and phosphate metabolites were tested. Exposure to cyanide, antimycin A and azide lead to a decrease in the activity of cyanide-sensitive respiration (CSR), and the ratio of core polyphosphates (PPc) and inorganic phosphates (Pi), which is a good indicator of the metabolic state of a cell. After 5 h of incubation, the activity of CSR returned to control values. For this, the recovery of cytochrome c oxid.ase (COX) was required. In addition, the PPc/Pi ratio started to recover shortly after initiation of COX recovery, but never reached control values. This led us to conclude that the regulation of polyphosphate (PPn) levels in the cell is tightly coupled to respiratory chain functioning. In addition, acutely applied cyanide caused two different responses, observed by P-31 NMR spectroscopy, that were probably mediated through the mechanism of glycolytic oscillations, triggered by the e...ffect of cyanide on mitochondria.
TY - JOUR
AU - Stanić, Marina
AU - Zivić, Miroslav
AU - Hadzibrahimović, Mirzeta
AU - Pajdic, Aleksandra
AU - Krizak, Strahinja
AU - Žižić, Milan
AU - Zakrzewska, Joanna
PY - 2014
UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/809
AB - The effects of long-term exposure (5 h) of Phycomyces blakesleeanus mycelium to 5 mM KCN on respiration and phosphate metabolites were tested. Exposure to cyanide, antimycin A and azide lead to a decrease in the activity of cyanide-sensitive respiration (CSR), and the ratio of core polyphosphates (PPc) and inorganic phosphates (Pi), which is a good indicator of the metabolic state of a cell. After 5 h of incubation, the activity of CSR returned to control values. For this, the recovery of cytochrome c oxid.ase (COX) was required. In addition, the PPc/Pi ratio started to recover shortly after initiation of COX recovery, but never reached control values. This led us to conclude that the regulation of polyphosphate (PPn) levels in the cell is tightly coupled to respiratory chain functioning. In addition, acutely applied cyanide caused two different responses, observed by P-31 NMR spectroscopy, that were probably mediated through the mechanism of glycolytic oscillations, triggered by the effect of cyanide on mitochondria.
PB - Srpsko biološko društvo, Beograd, i dr.
T2 - Archives of Biological Sciences
T1 - Effect of long-term cyanide exposure on cyanide-sensitive respiration and phosphate metabolism in the fungus phycomyces blakesleeanus
EP - 857
IS - 2
SP - 847
VL - 66
DO - 10.2298/ABS1402847S
ER -
@article{
author = "Stanić, Marina and Zivić, Miroslav and Hadzibrahimović, Mirzeta and Pajdic, Aleksandra and Krizak, Strahinja and Žižić, Milan and Zakrzewska, Joanna",
year = "2014",
abstract = "The effects of long-term exposure (5 h) of Phycomyces blakesleeanus mycelium to 5 mM KCN on respiration and phosphate metabolites were tested. Exposure to cyanide, antimycin A and azide lead to a decrease in the activity of cyanide-sensitive respiration (CSR), and the ratio of core polyphosphates (PPc) and inorganic phosphates (Pi), which is a good indicator of the metabolic state of a cell. After 5 h of incubation, the activity of CSR returned to control values. For this, the recovery of cytochrome c oxid.ase (COX) was required. In addition, the PPc/Pi ratio started to recover shortly after initiation of COX recovery, but never reached control values. This led us to conclude that the regulation of polyphosphate (PPn) levels in the cell is tightly coupled to respiratory chain functioning. In addition, acutely applied cyanide caused two different responses, observed by P-31 NMR spectroscopy, that were probably mediated through the mechanism of glycolytic oscillations, triggered by the effect of cyanide on mitochondria.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Effect of long-term cyanide exposure on cyanide-sensitive respiration and phosphate metabolism in the fungus phycomyces blakesleeanus",
pages = "857-847",
number = "2",
volume = "66",
doi = "10.2298/ABS1402847S"
}
Stanić, M., Zivić, M., Hadzibrahimović, M., Pajdic, A., Krizak, S., Žižić, M.,& Zakrzewska, J.. (2014). Effect of long-term cyanide exposure on cyanide-sensitive respiration and phosphate metabolism in the fungus phycomyces blakesleeanus. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 66(2), 847-857.
https://doi.org/10.2298/ABS1402847S
Stanić M, Zivić M, Hadzibrahimović M, Pajdic A, Krizak S, Žižić M, Zakrzewska J. Effect of long-term cyanide exposure on cyanide-sensitive respiration and phosphate metabolism in the fungus phycomyces blakesleeanus. in Archives of Biological Sciences. 2014;66(2):847-857.
doi:10.2298/ABS1402847S .
Stanić, Marina, Zivić, Miroslav, Hadzibrahimović, Mirzeta, Pajdic, Aleksandra, Krizak, Strahinja, Žižić, Milan, Zakrzewska, Joanna, "Effect of long-term cyanide exposure on cyanide-sensitive respiration and phosphate metabolism in the fungus phycomyces blakesleeanus" in Archives of Biological Sciences, 66, no. 2 (2014):847-857,
https://doi.org/10.2298/ABS1402847S . .
