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Imobilizacija hemijski modifikovane peroksidaze iz rena unutar aktiviranih alginatnih kuglica
Immobilization of chemically modified horseradish peroxidase within activated alginate beads
dc.creator | Spasojević, Dragica | |
dc.creator | Prokopijević, Miloš | |
dc.creator | Prodanović, Olivera | |
dc.creator | Pirtea, Marilen Gabriel | |
dc.creator | Radotić, Ksenija | |
dc.creator | Prodanović, Radivoje | |
dc.date.accessioned | 2022-04-05T14:52:14Z | |
dc.date.available | 2022-04-05T14:52:14Z | |
dc.date.issued | 2014 | |
dc.identifier.issn | 0367-598X | |
dc.identifier.uri | http://rimsi.imsi.bg.ac.rs/handle/123456789/808 | |
dc.description.abstract | Imobilizacija peroksidaze iz rena unutar alginatnih kuglica je poboljšana hemijskom modifikacijom enzima i polisaharidnih lanaca. Peroksidaza i alginat su oksidovani perjodatom i naknadno modifikovani etilendiaminom. Najveća specifična aktivnost od 0,43 U/ml gela i 81% vezane aktivnosti je dobijeno korišćenjem aminovane peroksidaze i alginata oksidovanog perjodatom. Imobilizovani enzim je zadržao 75% originalne aktivnosti nakon 2 dana inkubacije u 80% (v/v) dioksanu i imao je povećanu aktivnost pri baznim pH vrednostima u poređenju sa nativnim enzimom. Tokom višestruke upotrebe u šaržnom reaktoru za oksidaciju pirogalola imobilizovana peroksidaza je zadržala 75% početne aktivnosti. | sr |
dc.description.abstract | Immobilization of horseradish peroxidase (HRP) within alginate beads was enabled by chemical modification of the enzyme and polysaccharide chains. HRP and alginate were oxidized by periodate and subsequently modified with ethylenediamine. Highest specific activity of 0.43 U/ml of gel and 81% of bound enzyme activity was obtained using aminated HRP and alginate oxidized by periodate. Immobilized enzyme retained 75% of its original activity after 2 days of incubation in 80% (v/v) dioxane and had increased activity in basic solutions compared to native enzyme. During repeated use in batch reactor for pyrogallol oxidation immobilized peroxidase retained 75% of its original activity. | en |
dc.publisher | Savez hemijskih inženjera, Beograd | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173017/RS// | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172049/RS// | |
dc.rights | openAccess | |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/4.0/ | |
dc.source | Hemijska Industrija | |
dc.subject | Peroksidaza | sr |
dc.subject | Perjodat | sr |
dc.subject | Imobilizovan | sr |
dc.subject | Etilendiamin | sr |
dc.subject | peroxidase | en |
dc.subject | periodate | en |
dc.subject | immobilized | en |
dc.subject | ethylene diamine | en |
dc.title | Imobilizacija hemijski modifikovane peroksidaze iz rena unutar aktiviranih alginatnih kuglica | sr |
dc.title | Immobilization of chemically modified horseradish peroxidase within activated alginate beads | en |
dc.type | article | |
dc.rights.license | BY-NC-ND | |
dc.citation.epage | 122 | |
dc.citation.issue | 1 | |
dc.citation.other | 68(1): 117-122 | |
dc.citation.rank | M23 | |
dc.citation.spage | 117 | |
dc.citation.volume | 68 | |
dc.identifier.doi | 10.2298/HEMIND121122036S | |
dc.identifier.fulltext | http://rimsi.imsi.bg.ac.rs/bitstream/id/967/805.pdf | |
dc.identifier.scopus | 2-s2.0-84898767640 | |
dc.identifier.wos | 000335293800014 | |
dc.type.version | publishedVersion |