A bryozoan species may offer novel antioxidants with anti-carbon-dioxide anion radical activity

Abstract

The antiradical activity of the freshwater bryozoan Hyalinella punctata water extracts (two samples, seasonal collection) was evaluated by using electron paramagnetic resonance spectroscopy against hydroxyl (zOH), superoxide anion (center dot O-2(-)), methoxy (center dot CH2OH), carbon-dioxide anion (center dot CO2-), nitric-oxide (center dot NO) and 2,2-diphenyl-1-picrylhydrazyl (center dot DPPH) radicals. The extracts reduced the production of all tested radicals but to a varying degree. The better activity was observed against center dot CO2- and center dot CH2OH radicals (54 +/- 5% and 44 +/- 4%, and 58 +/- 6% and 22 +/- 2%, respectively) than towards center dot DPPH, center dot NO, center dot OH and center dot O-2(-) radicals (59 +/- 6% and 1.0 +/- 0.1%, 46 +/- 5% and 14 +/- 1%, 7.0 +/- 0.5% and 34 +/- 3%, and 33 +/- 3% and 0%, respectively). FTIR spectra of the both extracts indicate the presence of cyclic peptides and polypeptides which might be responsible for the observed activity. According to the experimental data obtained, H. punctata water extract may be considered as a novel promising resource of natural products with anti center dot CO2- radical activity.