In this work the genotoxic potential of water in three localities in Serbia, which differ by the nature and degree of pollution, was determined in tissues of European chub (Squalius cephalus L.) on monthly basis over the 2011/2012 year season using the alkaline comet assay. Specimen samples of chub were taken from Special Nature Reserve "Uvac", as control site, and Pestan and Beljanica Rivers, as polluted sites at Kolubara basin, surrounded with coal mines. Three tissues, blood, gills and liver were used for assessing the level of DNA damage. Analysis was done by software (Comet Assay IV). The control site at Reserve "Uvac" showed the lowest DNA damage values for all three tissues compared to Pestan and Beljanica. Blood has the lowest level of DNA damage in comparison with liver and gills. Decreased damage for all three tissues was observed at summer, while during the spring and autumn damage increased.
Keywords:
Water pollution / Genotoxicity / European chub / Comet assay
Source:
Environmental Toxicology and Pharmacology, 2014, 37, 3, 967-974
TY - JOUR
AU - Sunjog, Karolina
AU - Kolarević, Stoimir
AU - Kracun-Kolarević, Margareta
AU - Gačić, Zoran
AU - Szoric, S
AU - Đikanović, Vesna
AU - Lenhardt, Mirjana
AU - Vukovic-Gacic, Branka
PY - 2014
UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/765
AB - In this work the genotoxic potential of water in three localities in Serbia, which differ by the nature and degree of pollution, was determined in tissues of European chub (Squalius cephalus L.) on monthly basis over the 2011/2012 year season using the alkaline comet assay. Specimen samples of chub were taken from Special Nature Reserve "Uvac", as control site, and Pestan and Beljanica Rivers, as polluted sites at Kolubara basin, surrounded with coal mines. Three tissues, blood, gills and liver were used for assessing the level of DNA damage. Analysis was done by software (Comet Assay IV). The control site at Reserve "Uvac" showed the lowest DNA damage values for all three tissues compared to Pestan and Beljanica. Blood has the lowest level of DNA damage in comparison with liver and gills. Decreased damage for all three tissues was observed at summer, while during the spring and autumn damage increased.
PB - Elsevier, Amsterdam
T2 - Environmental Toxicology and Pharmacology
T1 - Variability in DNA damage of chub (Squalius cephalus L.) blood, gill and liver cells during the annual cycle
EP - 974
IS - 3
SP - 967
VL - 37
DO - 10.1016/j.etap.2014.03.010
ER -
@article{
author = "Sunjog, Karolina and Kolarević, Stoimir and Kracun-Kolarević, Margareta and Gačić, Zoran and Szoric, S and Đikanović, Vesna and Lenhardt, Mirjana and Vukovic-Gacic, Branka",
year = "2014",
abstract = "In this work the genotoxic potential of water in three localities in Serbia, which differ by the nature and degree of pollution, was determined in tissues of European chub (Squalius cephalus L.) on monthly basis over the 2011/2012 year season using the alkaline comet assay. Specimen samples of chub were taken from Special Nature Reserve "Uvac", as control site, and Pestan and Beljanica Rivers, as polluted sites at Kolubara basin, surrounded with coal mines. Three tissues, blood, gills and liver were used for assessing the level of DNA damage. Analysis was done by software (Comet Assay IV). The control site at Reserve "Uvac" showed the lowest DNA damage values for all three tissues compared to Pestan and Beljanica. Blood has the lowest level of DNA damage in comparison with liver and gills. Decreased damage for all three tissues was observed at summer, while during the spring and autumn damage increased.",
publisher = "Elsevier, Amsterdam",
journal = "Environmental Toxicology and Pharmacology",
title = "Variability in DNA damage of chub (Squalius cephalus L.) blood, gill and liver cells during the annual cycle",
pages = "974-967",
number = "3",
volume = "37",
doi = "10.1016/j.etap.2014.03.010"
}
Sunjog, K., Kolarević, S., Kracun-Kolarević, M., Gačić, Z., Szoric, S., Đikanović, V., Lenhardt, M.,& Vukovic-Gacic, B.. (2014). Variability in DNA damage of chub (Squalius cephalus L.) blood, gill and liver cells during the annual cycle. in Environmental Toxicology and Pharmacology
Elsevier, Amsterdam., 37(3), 967-974.
https://doi.org/10.1016/j.etap.2014.03.010
Sunjog K, Kolarević S, Kracun-Kolarević M, Gačić Z, Szoric S, Đikanović V, Lenhardt M, Vukovic-Gacic B. Variability in DNA damage of chub (Squalius cephalus L.) blood, gill and liver cells during the annual cycle. in Environmental Toxicology and Pharmacology. 2014;37(3):967-974.
doi:10.1016/j.etap.2014.03.010 .
Sunjog, Karolina, Kolarević, Stoimir, Kracun-Kolarević, Margareta, Gačić, Zoran, Szoric, S, Đikanović, Vesna, Lenhardt, Mirjana, Vukovic-Gacic, Branka, "Variability in DNA damage of chub (Squalius cephalus L.) blood, gill and liver cells during the annual cycle" in Environmental Toxicology and Pharmacology, 37, no. 3 (2014):967-974,
https://doi.org/10.1016/j.etap.2014.03.010 . .
