The biological and chemical basis of vanadium action in fungi is relatively poorly understood. In the present study, we investigate the influence of vanadate (V5+) on phosphate metabolism of Phycomyces blakesleeanus. Addition of V5+ caused increase of sugar phosphates signal intensities in P-31 NMR spectra in vivo. HPLC analysis of mycelial phosphate extracts demonstrated increased concentrations of glucose 6 phosphate, fructose 6 phosphate, fructose 1, 6 phosphate and glucose 1 phosphate after V5+ treatment. Influence of V5+ on the levels of fructose 2, 6 phosphate, glucosamine 6 phosphate and glucose 1, 6 phosphate (HPLC), and polyphosphates, UDPG and ATP (P-31 NMR) was also established. Increase of sugar phosphates content was not observed after addition of vanadyl (V4+), indicating that only vanadate influences its metabolism. Obtained results from in vivo experiments indicate catalytic/inhibitory vanadate action on enzymes involved in reactions of glycolysis and glycogenesis i.e.,... phosphoglucomutase, phosphofructokinase and glycogen phosphorylase in filamentous fungi.
TY - JOUR
AU - Žižić, Milan
AU - Zivić, Miroslav
AU - Maksimović, Vuk
AU - Stanić, Marina
AU - Krizak, Strahinja
AU - Cvetić-Antić, Tijana
AU - Zakrzewska, Joanna
PY - 2014
UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/750
AB - The biological and chemical basis of vanadium action in fungi is relatively poorly understood. In the present study, we investigate the influence of vanadate (V5+) on phosphate metabolism of Phycomyces blakesleeanus. Addition of V5+ caused increase of sugar phosphates signal intensities in P-31 NMR spectra in vivo. HPLC analysis of mycelial phosphate extracts demonstrated increased concentrations of glucose 6 phosphate, fructose 6 phosphate, fructose 1, 6 phosphate and glucose 1 phosphate after V5+ treatment. Influence of V5+ on the levels of fructose 2, 6 phosphate, glucosamine 6 phosphate and glucose 1, 6 phosphate (HPLC), and polyphosphates, UDPG and ATP (P-31 NMR) was also established. Increase of sugar phosphates content was not observed after addition of vanadyl (V4+), indicating that only vanadate influences its metabolism. Obtained results from in vivo experiments indicate catalytic/inhibitory vanadate action on enzymes involved in reactions of glycolysis and glycogenesis i.e., phosphoglucomutase, phosphofructokinase and glycogen phosphorylase in filamentous fungi.
PB - Public Library Science, San Francisco
T2 - PLoS One
T1 - Vanadate Influence on Metabolism of Sugar Phosphates in Fungus Phycomyces blakesleeanus
IS - 7
VL - 9
DO - 10.1371/journal.pone.0102849
ER -
@article{
author = "Žižić, Milan and Zivić, Miroslav and Maksimović, Vuk and Stanić, Marina and Krizak, Strahinja and Cvetić-Antić, Tijana and Zakrzewska, Joanna",
year = "2014",
abstract = "The biological and chemical basis of vanadium action in fungi is relatively poorly understood. In the present study, we investigate the influence of vanadate (V5+) on phosphate metabolism of Phycomyces blakesleeanus. Addition of V5+ caused increase of sugar phosphates signal intensities in P-31 NMR spectra in vivo. HPLC analysis of mycelial phosphate extracts demonstrated increased concentrations of glucose 6 phosphate, fructose 6 phosphate, fructose 1, 6 phosphate and glucose 1 phosphate after V5+ treatment. Influence of V5+ on the levels of fructose 2, 6 phosphate, glucosamine 6 phosphate and glucose 1, 6 phosphate (HPLC), and polyphosphates, UDPG and ATP (P-31 NMR) was also established. Increase of sugar phosphates content was not observed after addition of vanadyl (V4+), indicating that only vanadate influences its metabolism. Obtained results from in vivo experiments indicate catalytic/inhibitory vanadate action on enzymes involved in reactions of glycolysis and glycogenesis i.e., phosphoglucomutase, phosphofructokinase and glycogen phosphorylase in filamentous fungi.",
publisher = "Public Library Science, San Francisco",
journal = "PLoS One",
title = "Vanadate Influence on Metabolism of Sugar Phosphates in Fungus Phycomyces blakesleeanus",
number = "7",
volume = "9",
doi = "10.1371/journal.pone.0102849"
}
Žižić, M., Zivić, M., Maksimović, V., Stanić, M., Krizak, S., Cvetić-Antić, T.,& Zakrzewska, J.. (2014). Vanadate Influence on Metabolism of Sugar Phosphates in Fungus Phycomyces blakesleeanus. in PLoS One
Public Library Science, San Francisco., 9(7).
https://doi.org/10.1371/journal.pone.0102849
Žižić M, Zivić M, Maksimović V, Stanić M, Krizak S, Cvetić-Antić T, Zakrzewska J. Vanadate Influence on Metabolism of Sugar Phosphates in Fungus Phycomyces blakesleeanus. in PLoS One. 2014;9(7).
doi:10.1371/journal.pone.0102849 .
Žižić, Milan, Zivić, Miroslav, Maksimović, Vuk, Stanić, Marina, Krizak, Strahinja, Cvetić-Antić, Tijana, Zakrzewska, Joanna, "Vanadate Influence on Metabolism of Sugar Phosphates in Fungus Phycomyces blakesleeanus" in PLoS One, 9, no. 7 (2014),
https://doi.org/10.1371/journal.pone.0102849 . .
