Monitoring of DNA damage in haemocytes of freshwater mussel Sinanodonta woodiana sampled from the Velika Morava River in Serbia with the comet assay
Само за регистроване кориснике
2013
Аутори
Kolarević, StoimirKnezevic-Vukcević, Jelena
Paunović, Momir
Kracun, Margareta
Vasiljević, Bozica
Tomović, Jelena M
Vukovic-Gacic, Branka
Gačić, Zoran
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
This study was undertaken to investigate the potential of the freshwater mussel Sinanodonta woodiana for detection of genotoxic pollution of the environment. Study was performed at two sites in the Velika Morava River, from May 2010 to February 2011. The alkaline comet assay on haemocytes was used, and the olive tail moment (OTM) was chosen as a measure of DNA damage. The specimens held on acclimation under controlled laboratory conditions for 10 d were used as a control. Chemical analysis revealed the presence of phosphates and increased concentrations of zinc, copper and nickel at both sites during the entire sampling period. The values of OTM in mussels collected from the environment, significantly correlated with the concentration of zinc (r = 0.6248), temperature (r = 0.7006) and dissolved oxygen (r = 0.7738). Seasonal variations in genotoxic response were observed, with the highest OTM values obtained during summer months. Preliminary results of the in vitro study indicated the e...ffect of water temperature on genotoxic response to zinc and cadmium in S. woodiana suggesting that the presence of genotoxic pollutants during months with lower temperature could be under-estimated. Obtained results indicate that S. woodiana could be a valuable tool for active biomonitoring of aquatic environments and emphasizes the importance of seasonal genotoxic monitoring with this organism.
Кључне речи:
Velika morava river / Sinanodonta woodiana / Haemocytes / Comet assayИзвор:
Chemosphere, 2013, 93, 2, 243-251Издавач:
- Pergamon-Elsevier Science Ltd, Oxford
Финансирање / пројекти:
DOI: 10.1016/j.chemosphere.2013.04.073
ISSN: 0045-6535
PubMed: 23722166
WoS: 000324667700007
Scopus: 2-s2.0-84882874015
Институција/група
Institut za multidisciplinarna istraživanjaTY - JOUR AU - Kolarević, Stoimir AU - Knezevic-Vukcević, Jelena AU - Paunović, Momir AU - Kracun, Margareta AU - Vasiljević, Bozica AU - Tomović, Jelena M AU - Vukovic-Gacic, Branka AU - Gačić, Zoran PY - 2013 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/703 AB - This study was undertaken to investigate the potential of the freshwater mussel Sinanodonta woodiana for detection of genotoxic pollution of the environment. Study was performed at two sites in the Velika Morava River, from May 2010 to February 2011. The alkaline comet assay on haemocytes was used, and the olive tail moment (OTM) was chosen as a measure of DNA damage. The specimens held on acclimation under controlled laboratory conditions for 10 d were used as a control. Chemical analysis revealed the presence of phosphates and increased concentrations of zinc, copper and nickel at both sites during the entire sampling period. The values of OTM in mussels collected from the environment, significantly correlated with the concentration of zinc (r = 0.6248), temperature (r = 0.7006) and dissolved oxygen (r = 0.7738). Seasonal variations in genotoxic response were observed, with the highest OTM values obtained during summer months. Preliminary results of the in vitro study indicated the effect of water temperature on genotoxic response to zinc and cadmium in S. woodiana suggesting that the presence of genotoxic pollutants during months with lower temperature could be under-estimated. Obtained results indicate that S. woodiana could be a valuable tool for active biomonitoring of aquatic environments and emphasizes the importance of seasonal genotoxic monitoring with this organism. PB - Pergamon-Elsevier Science Ltd, Oxford T2 - Chemosphere T1 - Monitoring of DNA damage in haemocytes of freshwater mussel Sinanodonta woodiana sampled from the Velika Morava River in Serbia with the comet assay EP - 251 IS - 2 SP - 243 VL - 93 DO - 10.1016/j.chemosphere.2013.04.073 ER -
@article{ author = "Kolarević, Stoimir and Knezevic-Vukcević, Jelena and Paunović, Momir and Kracun, Margareta and Vasiljević, Bozica and Tomović, Jelena M and Vukovic-Gacic, Branka and Gačić, Zoran", year = "2013", abstract = "This study was undertaken to investigate the potential of the freshwater mussel Sinanodonta woodiana for detection of genotoxic pollution of the environment. Study was performed at two sites in the Velika Morava River, from May 2010 to February 2011. The alkaline comet assay on haemocytes was used, and the olive tail moment (OTM) was chosen as a measure of DNA damage. The specimens held on acclimation under controlled laboratory conditions for 10 d were used as a control. Chemical analysis revealed the presence of phosphates and increased concentrations of zinc, copper and nickel at both sites during the entire sampling period. The values of OTM in mussels collected from the environment, significantly correlated with the concentration of zinc (r = 0.6248), temperature (r = 0.7006) and dissolved oxygen (r = 0.7738). Seasonal variations in genotoxic response were observed, with the highest OTM values obtained during summer months. Preliminary results of the in vitro study indicated the effect of water temperature on genotoxic response to zinc and cadmium in S. woodiana suggesting that the presence of genotoxic pollutants during months with lower temperature could be under-estimated. Obtained results indicate that S. woodiana could be a valuable tool for active biomonitoring of aquatic environments and emphasizes the importance of seasonal genotoxic monitoring with this organism.", publisher = "Pergamon-Elsevier Science Ltd, Oxford", journal = "Chemosphere", title = "Monitoring of DNA damage in haemocytes of freshwater mussel Sinanodonta woodiana sampled from the Velika Morava River in Serbia with the comet assay", pages = "251-243", number = "2", volume = "93", doi = "10.1016/j.chemosphere.2013.04.073" }
Kolarević, S., Knezevic-Vukcević, J., Paunović, M., Kracun, M., Vasiljević, B., Tomović, J. M., Vukovic-Gacic, B.,& Gačić, Z.. (2013). Monitoring of DNA damage in haemocytes of freshwater mussel Sinanodonta woodiana sampled from the Velika Morava River in Serbia with the comet assay. in Chemosphere Pergamon-Elsevier Science Ltd, Oxford., 93(2), 243-251. https://doi.org/10.1016/j.chemosphere.2013.04.073
Kolarević S, Knezevic-Vukcević J, Paunović M, Kracun M, Vasiljević B, Tomović JM, Vukovic-Gacic B, Gačić Z. Monitoring of DNA damage in haemocytes of freshwater mussel Sinanodonta woodiana sampled from the Velika Morava River in Serbia with the comet assay. in Chemosphere. 2013;93(2):243-251. doi:10.1016/j.chemosphere.2013.04.073 .
Kolarević, Stoimir, Knezevic-Vukcević, Jelena, Paunović, Momir, Kracun, Margareta, Vasiljević, Bozica, Tomović, Jelena M, Vukovic-Gacic, Branka, Gačić, Zoran, "Monitoring of DNA damage in haemocytes of freshwater mussel Sinanodonta woodiana sampled from the Velika Morava River in Serbia with the comet assay" in Chemosphere, 93, no. 2 (2013):243-251, https://doi.org/10.1016/j.chemosphere.2013.04.073 . .