Приказ основних података о документу

dc.creatorJovanović, Katarina K
dc.creatorSavić, Aleksandar G
dc.creatorJanković, Radmila N
dc.creatorRadulović, Sinisa S
dc.creatorSpasić, Slađana
dc.creatorRadotić, Ksenija
dc.date.accessioned2022-04-05T14:45:04Z
dc.date.available2022-04-05T14:45:04Z
dc.date.issued2013
dc.identifier.issn0306-9877
dc.identifier.urihttp://rimsi.imsi.bg.ac.rs/handle/123456789/702
dc.description.abstractThe key method for therapies of various cancer types could be the molecular-targeted therapy, based on individual gene profile for each patient. One of the main procedures used for genetic testing is the real-time polymerase chain reaction (real-time PCR). Physical principle behind real-time PCR procedure is the fluorescence. Fluorescence labeled probes (primers) is attached to quenchers. Upon reaction of polymerization, quenchers are removed, and the fluorescence emission intensity increases in time. Emission spectra shape and its maximum position can differ if the fluorophore was present in different microenvironment. That property is widely exploited in fluorescence spectroscopy and chromatography. This paper, for the first time, describes utilization of full spectroscopic potential of multichannel excitation/emission filter sets in real-time PCR device. Instead of monitoring fluorescence intensity in time for a single fluorescence emission channel, the ratio values of three different kinetics curves were calculated and analyzed by applying k-means clustering and dendrogram analysis. Obtained results have shown that described analytical improvement provides identification of nine different groups of mutations if the commercial QIAGEN (R) EGFR PCR Kit was used. Method can be applied to any kit, capable to simultaneously detect several different mutations.en
dc.publisherChurchill Livingstone, Edinburgh
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173017/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173045/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/41026/RS//
dc.rightsrestrictedAccess
dc.sourceMedical Hypotheses
dc.subjectDetection of DNA mutations
dc.subjectexcitation fluorescence
dc.subjectemission fluorescence
dc.subjectreal-time PCR
dc.titleDetection of DNA mutations based on analysis of multiple wavelength excitation/emission fluorescence kinetics curves in real-time PCRen
dc.typearticle
dc.rights.licenseARR
dc.citation.epage379
dc.citation.issue4
dc.citation.other80(4): 376-379
dc.citation.rankM23
dc.citation.spage376
dc.citation.volume80
dc.identifier.doi10.1016/j.mehy.2013.01.004
dc.identifier.pmid23352288
dc.identifier.scopus2-s2.0-84875404058
dc.identifier.wos000317164500009
dc.type.versionpublishedVersion


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Приказ основних података о документу