Enhancement in statistical and image analysis for in situ mu SXRF studies of elemental distribution and co-localization, using Dioscorea balcanica
2013
Аутори
Ducic, TanjaBorchert, Manuela
Savić, Aleksandar G
Kalauzi, Aleksandar
Mitrović, Aleksandra Lj.
Radotić, Ksenija
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Synchrotron-based X-ray microfluorescence (mu SXRF) is an analytical method suitable for in situ investigation of the distribution of micronutrient and macronutrient elements in several-micrometres-thick unstained biological samples, e. g. single cells and tissues. Elements are mapped and quantified at sub-p. p. m. concentrations. In this study the quantity, distribution and grouping/co-localization of various elements have been identified in straight and twisted internodes of the stems of the monocotyledonous climber D. balcanica Kosanin. Three different statistical methods were employed to analyse the macronutrient and micronutrient distributions and co-localization. Macronutrient elements (K, P, Ca, Cl) are distributed homogeneously in both straight and twisted internodes. Micronutrient elements are mostly grouped in the vasculature and in the sclerenchyma cell layer. In addition, co-localization of micronutrient elements is much more prominent in twisted than in straight internodes.... These image analyses and statistical methods provided very similar outcomes and could be applied to various types of biological samples imaged by mSXRF.
Кључне речи:
X-ray fluorescence microscopy / statistical analysis / image analysis / Dioscorea balcanica KosaninИзвор:
Journal of Synchrotron Radiation, 2013, 20, 339-346Издавач:
- Wiley-Blackwell, Hoboken
Финансирање / пројекти:
- Испитивања односа структура-функција у ћелијском зиду биљака и измене структуре зида ензимским инжењерингом (RS-MESTD-Basic Research (BR or ON)-173017)
- II-20080239]
DOI: 10.1107/S0909049512050170
ISSN: 0909-0495
PubMed: 23412492
WoS: 000314929700018
Scopus: 2-s2.0-84874088216
Институција/група
Institut za multidisciplinarna istraživanjaTY - JOUR AU - Ducic, Tanja AU - Borchert, Manuela AU - Savić, Aleksandar G AU - Kalauzi, Aleksandar AU - Mitrović, Aleksandra Lj. AU - Radotić, Ksenija PY - 2013 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/696 AB - Synchrotron-based X-ray microfluorescence (mu SXRF) is an analytical method suitable for in situ investigation of the distribution of micronutrient and macronutrient elements in several-micrometres-thick unstained biological samples, e. g. single cells and tissues. Elements are mapped and quantified at sub-p. p. m. concentrations. In this study the quantity, distribution and grouping/co-localization of various elements have been identified in straight and twisted internodes of the stems of the monocotyledonous climber D. balcanica Kosanin. Three different statistical methods were employed to analyse the macronutrient and micronutrient distributions and co-localization. Macronutrient elements (K, P, Ca, Cl) are distributed homogeneously in both straight and twisted internodes. Micronutrient elements are mostly grouped in the vasculature and in the sclerenchyma cell layer. In addition, co-localization of micronutrient elements is much more prominent in twisted than in straight internodes. These image analyses and statistical methods provided very similar outcomes and could be applied to various types of biological samples imaged by mSXRF. PB - Wiley-Blackwell, Hoboken T2 - Journal of Synchrotron Radiation T1 - Enhancement in statistical and image analysis for in situ mu SXRF studies of elemental distribution and co-localization, using Dioscorea balcanica EP - 346 SP - 339 VL - 20 DO - 10.1107/S0909049512050170 ER -
@article{ author = "Ducic, Tanja and Borchert, Manuela and Savić, Aleksandar G and Kalauzi, Aleksandar and Mitrović, Aleksandra Lj. and Radotić, Ksenija", year = "2013", abstract = "Synchrotron-based X-ray microfluorescence (mu SXRF) is an analytical method suitable for in situ investigation of the distribution of micronutrient and macronutrient elements in several-micrometres-thick unstained biological samples, e. g. single cells and tissues. Elements are mapped and quantified at sub-p. p. m. concentrations. In this study the quantity, distribution and grouping/co-localization of various elements have been identified in straight and twisted internodes of the stems of the monocotyledonous climber D. balcanica Kosanin. Three different statistical methods were employed to analyse the macronutrient and micronutrient distributions and co-localization. Macronutrient elements (K, P, Ca, Cl) are distributed homogeneously in both straight and twisted internodes. Micronutrient elements are mostly grouped in the vasculature and in the sclerenchyma cell layer. In addition, co-localization of micronutrient elements is much more prominent in twisted than in straight internodes. These image analyses and statistical methods provided very similar outcomes and could be applied to various types of biological samples imaged by mSXRF.", publisher = "Wiley-Blackwell, Hoboken", journal = "Journal of Synchrotron Radiation", title = "Enhancement in statistical and image analysis for in situ mu SXRF studies of elemental distribution and co-localization, using Dioscorea balcanica", pages = "346-339", volume = "20", doi = "10.1107/S0909049512050170" }
Ducic, T., Borchert, M., Savić, A. G., Kalauzi, A., Mitrović, A. Lj.,& Radotić, K.. (2013). Enhancement in statistical and image analysis for in situ mu SXRF studies of elemental distribution and co-localization, using Dioscorea balcanica. in Journal of Synchrotron Radiation Wiley-Blackwell, Hoboken., 20, 339-346. https://doi.org/10.1107/S0909049512050170
Ducic T, Borchert M, Savić AG, Kalauzi A, Mitrović AL, Radotić K. Enhancement in statistical and image analysis for in situ mu SXRF studies of elemental distribution and co-localization, using Dioscorea balcanica. in Journal of Synchrotron Radiation. 2013;20:339-346. doi:10.1107/S0909049512050170 .
Ducic, Tanja, Borchert, Manuela, Savić, Aleksandar G, Kalauzi, Aleksandar, Mitrović, Aleksandra Lj., Radotić, Ksenija, "Enhancement in statistical and image analysis for in situ mu SXRF studies of elemental distribution and co-localization, using Dioscorea balcanica" in Journal of Synchrotron Radiation, 20 (2013):339-346, https://doi.org/10.1107/S0909049512050170 . .