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Pollen morphology, viability, and germination of Prunus domestica cv. Pozegaca

Authorized Users Only
2013
Authors
Calic, Dusica D
Devrnja, Nina M
Kostić, Igor
Kostic, Miroslav
Article (Published version)
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Abstract
The pollen morphology and exine ornamentation of an important autochthon plum cultivar (Prunus domestica cv. Pozegaca L), which has horticultural, medicinal and alimentary uses, was examined in detail using both light and scanning electron microscope (SEM). Acetocarmine and fluorescein diacetate were used to assess the pollen viability of four Pozegaca plum genotypes. Pollen viability varied from 67% in genotype Pdp1 to 99% in genotype Pdp4. Also, pollen nucleus status was determined, and binucleate mature pollens were detected. The effect of polyethylene glycol-PEG (10, 15 and 20%, w/v) on pollen germination and tube growth was evaluated. Overall, the inclusion of PEG in the medium improved both pollen germination and tube growth. Regardless of the PEG concentration genotype has an effect on germination and length of pollen tubes. Genotype has a pronounced effect on germination and length of pollen tubes, regardless of the PEG concentration used. Genotype Pdp4 had the highest germinat...ion percentage and the highest pollen tube length on all media when compared to the other three genotypes. The highest pollen germination (96%) and a tube length (822 mu m) of genotype PdP4 was attained in media with 20% PEG.

Keywords:
Viability / Pozegaca plum / Pollen tube growth / Pollen nuclear status / Pollen morphology / Pollen germination
Source:
Scientia Horticulturae, 2013, 155, 118-122
Publisher:
  • Elsevier, Amsterdam
Funding / projects:
  • Biotechnology in vitro - crop, medicinal and endangered plant species (RS-173015)

DOI: 10.1016/j.scienta.2013.03.017

ISSN: 0304-4238

WoS: 000319240500018

Scopus: 2-s2.0-84876380397
[ Google Scholar ]
25
9
URI
http://rimsi.imsi.bg.ac.rs/handle/123456789/691
Collections
  • Radovi istraživača / Researchers’ publications
Institution/Community
Institut za multidisciplinarna istraživanja
TY  - JOUR
AU  - Calic, Dusica D
AU  - Devrnja, Nina M
AU  - Kostić, Igor
AU  - Kostic, Miroslav
PY  - 2013
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/691
AB  - The pollen morphology and exine ornamentation of an important autochthon plum cultivar (Prunus domestica cv. Pozegaca L), which has horticultural, medicinal and alimentary uses, was examined in detail using both light and scanning electron microscope (SEM). Acetocarmine and fluorescein diacetate were used to assess the pollen viability of four Pozegaca plum genotypes. Pollen viability varied from 67% in genotype Pdp1 to 99% in genotype Pdp4. Also, pollen nucleus status was determined, and binucleate mature pollens were detected. The effect of polyethylene glycol-PEG (10, 15 and 20%, w/v) on pollen germination and tube growth was evaluated. Overall, the inclusion of PEG in the medium improved both pollen germination and tube growth. Regardless of the PEG concentration genotype has an effect on germination and length of pollen tubes. Genotype has a pronounced effect on germination and length of pollen tubes, regardless of the PEG concentration used. Genotype Pdp4 had the highest germination percentage and the highest pollen tube length on all media when compared to the other three genotypes. The highest pollen germination (96%) and a tube length (822 mu m) of genotype PdP4 was attained in media with 20% PEG.
PB  - Elsevier, Amsterdam
T2  - Scientia Horticulturae
T1  - Pollen morphology, viability, and germination of Prunus domestica cv. Pozegaca
EP  - 122
SP  - 118
VL  - 155
DO  - 10.1016/j.scienta.2013.03.017
ER  - 
@article{
author = "Calic, Dusica D and Devrnja, Nina M and Kostić, Igor and Kostic, Miroslav",
year = "2013",
abstract = "The pollen morphology and exine ornamentation of an important autochthon plum cultivar (Prunus domestica cv. Pozegaca L), which has horticultural, medicinal and alimentary uses, was examined in detail using both light and scanning electron microscope (SEM). Acetocarmine and fluorescein diacetate were used to assess the pollen viability of four Pozegaca plum genotypes. Pollen viability varied from 67% in genotype Pdp1 to 99% in genotype Pdp4. Also, pollen nucleus status was determined, and binucleate mature pollens were detected. The effect of polyethylene glycol-PEG (10, 15 and 20%, w/v) on pollen germination and tube growth was evaluated. Overall, the inclusion of PEG in the medium improved both pollen germination and tube growth. Regardless of the PEG concentration genotype has an effect on germination and length of pollen tubes. Genotype has a pronounced effect on germination and length of pollen tubes, regardless of the PEG concentration used. Genotype Pdp4 had the highest germination percentage and the highest pollen tube length on all media when compared to the other three genotypes. The highest pollen germination (96%) and a tube length (822 mu m) of genotype PdP4 was attained in media with 20% PEG.",
publisher = "Elsevier, Amsterdam",
journal = "Scientia Horticulturae",
title = "Pollen morphology, viability, and germination of Prunus domestica cv. Pozegaca",
pages = "122-118",
volume = "155",
doi = "10.1016/j.scienta.2013.03.017"
}
Calic, D. D., Devrnja, N. M., Kostić, I.,& Kostic, M.. (2013). Pollen morphology, viability, and germination of Prunus domestica cv. Pozegaca. in Scientia Horticulturae
Elsevier, Amsterdam., 155, 118-122.
https://doi.org/10.1016/j.scienta.2013.03.017
Calic DD, Devrnja NM, Kostić I, Kostic M. Pollen morphology, viability, and germination of Prunus domestica cv. Pozegaca. in Scientia Horticulturae. 2013;155:118-122.
doi:10.1016/j.scienta.2013.03.017 .
Calic, Dusica D, Devrnja, Nina M, Kostić, Igor, Kostic, Miroslav, "Pollen morphology, viability, and germination of Prunus domestica cv. Pozegaca" in Scientia Horticulturae, 155 (2013):118-122,
https://doi.org/10.1016/j.scienta.2013.03.017 . .

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