Pollen morphology, viability, and germination of Prunus domestica cv. Pozegaca
Abstract
The pollen morphology and exine ornamentation of an important autochthon plum cultivar (Prunus domestica cv. Pozegaca L), which has horticultural, medicinal and alimentary uses, was examined in detail using both light and scanning electron microscope (SEM). Acetocarmine and fluorescein diacetate were used to assess the pollen viability of four Pozegaca plum genotypes. Pollen viability varied from 67% in genotype Pdp1 to 99% in genotype Pdp4. Also, pollen nucleus status was determined, and binucleate mature pollens were detected. The effect of polyethylene glycol-PEG (10, 15 and 20%, w/v) on pollen germination and tube growth was evaluated. Overall, the inclusion of PEG in the medium improved both pollen germination and tube growth. Regardless of the PEG concentration genotype has an effect on germination and length of pollen tubes. Genotype has a pronounced effect on germination and length of pollen tubes, regardless of the PEG concentration used. Genotype Pdp4 had the highest germinat...ion percentage and the highest pollen tube length on all media when compared to the other three genotypes. The highest pollen germination (96%) and a tube length (822 mu m) of genotype PdP4 was attained in media with 20% PEG.
Keywords:
Viability / Pozegaca plum / Pollen tube growth / Pollen nuclear status / Pollen morphology / Pollen germinationSource:
Scientia Horticulturae, 2013, 155, 118-122Publisher:
- Elsevier, Amsterdam
Funding / projects:
DOI: 10.1016/j.scienta.2013.03.017
ISSN: 0304-4238
WoS: 000319240500018
Scopus: 2-s2.0-84876380397
Collections
Institution/Community
Institut za multidisciplinarna istraživanjaTY - JOUR AU - Calic, Dusica D AU - Devrnja, Nina M AU - Kostić, Igor AU - Kostic, Miroslav PY - 2013 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/691 AB - The pollen morphology and exine ornamentation of an important autochthon plum cultivar (Prunus domestica cv. Pozegaca L), which has horticultural, medicinal and alimentary uses, was examined in detail using both light and scanning electron microscope (SEM). Acetocarmine and fluorescein diacetate were used to assess the pollen viability of four Pozegaca plum genotypes. Pollen viability varied from 67% in genotype Pdp1 to 99% in genotype Pdp4. Also, pollen nucleus status was determined, and binucleate mature pollens were detected. The effect of polyethylene glycol-PEG (10, 15 and 20%, w/v) on pollen germination and tube growth was evaluated. Overall, the inclusion of PEG in the medium improved both pollen germination and tube growth. Regardless of the PEG concentration genotype has an effect on germination and length of pollen tubes. Genotype has a pronounced effect on germination and length of pollen tubes, regardless of the PEG concentration used. Genotype Pdp4 had the highest germination percentage and the highest pollen tube length on all media when compared to the other three genotypes. The highest pollen germination (96%) and a tube length (822 mu m) of genotype PdP4 was attained in media with 20% PEG. PB - Elsevier, Amsterdam T2 - Scientia Horticulturae T1 - Pollen morphology, viability, and germination of Prunus domestica cv. Pozegaca EP - 122 SP - 118 VL - 155 DO - 10.1016/j.scienta.2013.03.017 ER -
@article{ author = "Calic, Dusica D and Devrnja, Nina M and Kostić, Igor and Kostic, Miroslav", year = "2013", abstract = "The pollen morphology and exine ornamentation of an important autochthon plum cultivar (Prunus domestica cv. Pozegaca L), which has horticultural, medicinal and alimentary uses, was examined in detail using both light and scanning electron microscope (SEM). Acetocarmine and fluorescein diacetate were used to assess the pollen viability of four Pozegaca plum genotypes. Pollen viability varied from 67% in genotype Pdp1 to 99% in genotype Pdp4. Also, pollen nucleus status was determined, and binucleate mature pollens were detected. The effect of polyethylene glycol-PEG (10, 15 and 20%, w/v) on pollen germination and tube growth was evaluated. Overall, the inclusion of PEG in the medium improved both pollen germination and tube growth. Regardless of the PEG concentration genotype has an effect on germination and length of pollen tubes. Genotype has a pronounced effect on germination and length of pollen tubes, regardless of the PEG concentration used. Genotype Pdp4 had the highest germination percentage and the highest pollen tube length on all media when compared to the other three genotypes. The highest pollen germination (96%) and a tube length (822 mu m) of genotype PdP4 was attained in media with 20% PEG.", publisher = "Elsevier, Amsterdam", journal = "Scientia Horticulturae", title = "Pollen morphology, viability, and germination of Prunus domestica cv. Pozegaca", pages = "122-118", volume = "155", doi = "10.1016/j.scienta.2013.03.017" }
Calic, D. D., Devrnja, N. M., Kostić, I.,& Kostic, M.. (2013). Pollen morphology, viability, and germination of Prunus domestica cv. Pozegaca. in Scientia Horticulturae Elsevier, Amsterdam., 155, 118-122. https://doi.org/10.1016/j.scienta.2013.03.017
Calic DD, Devrnja NM, Kostić I, Kostic M. Pollen morphology, viability, and germination of Prunus domestica cv. Pozegaca. in Scientia Horticulturae. 2013;155:118-122. doi:10.1016/j.scienta.2013.03.017 .
Calic, Dusica D, Devrnja, Nina M, Kostić, Igor, Kostic, Miroslav, "Pollen morphology, viability, and germination of Prunus domestica cv. Pozegaca" in Scientia Horticulturae, 155 (2013):118-122, https://doi.org/10.1016/j.scienta.2013.03.017 . .
Related items
Showing items related by title, author, creator and subject.
-
The use of fluorescence microscopy for classification of pollen grains
Stanković, Mira; Simonović Radosavljević, Jasna; Đikanović, Daniela; Spasojević, Dragica; Radotić, Ksenija (University of Belgrade, Institute for Biological Research “Siniša Stanković”, National Institute of Republic of Serbia, Serbian Society for Microscopy, Serbia, 2019) -
Explainable AI for unveiling deep learning pollen classification model based on fusion of scattered light patterns and fluorescence spectroscopy
Brdar, Sanja; Panic, Marko; Matavulj, Predrag; Stanković, Mira; Bartolić, Dragana; Sikoparija, Branko (Springer Nature, 2023) -
Chemical composition, bioactive compounds, antioxidant capacity and stability of floral maize (Zea mays L.) pollen
Zilic, Sladana; Vancetavić, Jelena; Janković, Marijana; Maksimović, Vuk (Elsevier, Amsterdam, 2014)