To test the hypothesis that truffle-associated bacteria may improve truffle nutrition, we isolated bacteria from white truffle ascocarps and tested Actinobacteria for their ability to solubilise phosphate and iron, nutrients that have limited availability in white truffle grounds. Two isolates with sequence similarities to Curtobacterium flaccumfaciens and Rhodococcus sp. were characterized in detail. Both solubilised Ca-3(PO4)(2) in a way that was dependent on the nitrogen and carbon sources present. Neither strain broke down phytate, but both produced chelating compounds, performed ammonification, and broke down beta-glucan. Additionally, C. flaccumfaciens decomposed chitin, pectin, lipids and proteins, while Rhodococcus sp. exhibited urease activity. Three potentially fungicolous fungi were isolated from diseased white truffle ascocarps and bioassayed against the isolated Actinobacteria. The Rhodococcus isolate inhibited Verticillium leptobactrum, neither bacterium affected Clonosta...chys rosea, while both isolates promoted growth of Trichoderma sp. The results suggest that Actinobacteria might be involved in improving truffle nutrition, ascocarp degradation and establishing relationships with other soil fungi.
TY - JOUR
AU - Pavić, Aleksandar B
AU - Stanković, Slaviša
AU - Saljnikov, Elmira
AU - Krueger, Dirk
AU - Buscot, Francois
AU - Tarkka, Mika
AU - Marjanović, Žaklina
PY - 2013
UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/684
AB - To test the hypothesis that truffle-associated bacteria may improve truffle nutrition, we isolated bacteria from white truffle ascocarps and tested Actinobacteria for their ability to solubilise phosphate and iron, nutrients that have limited availability in white truffle grounds. Two isolates with sequence similarities to Curtobacterium flaccumfaciens and Rhodococcus sp. were characterized in detail. Both solubilised Ca-3(PO4)(2) in a way that was dependent on the nitrogen and carbon sources present. Neither strain broke down phytate, but both produced chelating compounds, performed ammonification, and broke down beta-glucan. Additionally, C. flaccumfaciens decomposed chitin, pectin, lipids and proteins, while Rhodococcus sp. exhibited urease activity. Three potentially fungicolous fungi were isolated from diseased white truffle ascocarps and bioassayed against the isolated Actinobacteria. The Rhodococcus isolate inhibited Verticillium leptobactrum, neither bacterium affected Clonostachys rosea, while both isolates promoted growth of Trichoderma sp. The results suggest that Actinobacteria might be involved in improving truffle nutrition, ascocarp degradation and establishing relationships with other soil fungi.
PB - Elsevier Sci Ltd, Oxford
T2 - Fungal Ecology
T1 - Actinobacteria may influence white truffle (Tuber magnatum Pico) nutrition, ascocarp degradation and interactions with other soil fungi
EP - 538
IS - 6
SP - 527
VL - 6
DO - 10.1016/j.funeco.2013.05.006
ER -
@article{
author = "Pavić, Aleksandar B and Stanković, Slaviša and Saljnikov, Elmira and Krueger, Dirk and Buscot, Francois and Tarkka, Mika and Marjanović, Žaklina",
year = "2013",
abstract = "To test the hypothesis that truffle-associated bacteria may improve truffle nutrition, we isolated bacteria from white truffle ascocarps and tested Actinobacteria for their ability to solubilise phosphate and iron, nutrients that have limited availability in white truffle grounds. Two isolates with sequence similarities to Curtobacterium flaccumfaciens and Rhodococcus sp. were characterized in detail. Both solubilised Ca-3(PO4)(2) in a way that was dependent on the nitrogen and carbon sources present. Neither strain broke down phytate, but both produced chelating compounds, performed ammonification, and broke down beta-glucan. Additionally, C. flaccumfaciens decomposed chitin, pectin, lipids and proteins, while Rhodococcus sp. exhibited urease activity. Three potentially fungicolous fungi were isolated from diseased white truffle ascocarps and bioassayed against the isolated Actinobacteria. The Rhodococcus isolate inhibited Verticillium leptobactrum, neither bacterium affected Clonostachys rosea, while both isolates promoted growth of Trichoderma sp. The results suggest that Actinobacteria might be involved in improving truffle nutrition, ascocarp degradation and establishing relationships with other soil fungi.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Fungal Ecology",
title = "Actinobacteria may influence white truffle (Tuber magnatum Pico) nutrition, ascocarp degradation and interactions with other soil fungi",
pages = "538-527",
number = "6",
volume = "6",
doi = "10.1016/j.funeco.2013.05.006"
}
Pavić, A. B., Stanković, S., Saljnikov, E., Krueger, D., Buscot, F., Tarkka, M.,& Marjanović, Ž.. (2013). Actinobacteria may influence white truffle (Tuber magnatum Pico) nutrition, ascocarp degradation and interactions with other soil fungi. in Fungal Ecology
Elsevier Sci Ltd, Oxford., 6(6), 527-538.
https://doi.org/10.1016/j.funeco.2013.05.006
Pavić AB, Stanković S, Saljnikov E, Krueger D, Buscot F, Tarkka M, Marjanović Ž. Actinobacteria may influence white truffle (Tuber magnatum Pico) nutrition, ascocarp degradation and interactions with other soil fungi. in Fungal Ecology. 2013;6(6):527-538.
doi:10.1016/j.funeco.2013.05.006 .
Pavić, Aleksandar B, Stanković, Slaviša, Saljnikov, Elmira, Krueger, Dirk, Buscot, Francois, Tarkka, Mika, Marjanović, Žaklina, "Actinobacteria may influence white truffle (Tuber magnatum Pico) nutrition, ascocarp degradation and interactions with other soil fungi" in Fungal Ecology, 6, no. 6 (2013):527-538,
https://doi.org/10.1016/j.funeco.2013.05.006 . .
