Antioxidant (AO) activity of extracts of hop cones (Serbian domestic varieties) and commercial hop products (Saaz, Spalter, Spalter select, and Magnum pellets) was determined by parallel application of recently developed direct current (DC) polarographic and widely used DPPH assay. Correlations between 2,2-diphenyl-1-picrylhydrazyl radical (DPPH) scavenging and total phenolics (TPC) determined by the Folin-Ciocalteu assay (FC) (0.99), and between H2O2 scavenging,: (HPS) and humulone content (H) determined by conductometric method (0.94), total resins (TR) (0.85), and hop storage index (HIS) (-0.90), were found statistically significant at p lt 0.05 level while complete lack of HPS correlation with TPC and DPPH was observed. To obtain an insight into differences between results of AO assays applied, activity of individual compounds, prevalent hop phenolics, and bitter acids was determined. By far superior HPS activity of humulone was followed by catechin, quercetin, xanthohumol, lupul...one, and rutin. In contrast, DPPH scavenging activity of phenolics (quercetin > catechin > rutin > xantohumol) was found substantially higher than activity of bitter acids. According to ferric reducing antioxidant power (FRAP) and scavenging of 2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS), higher AO activity was ascribed to phenolics, while almost neglecting humulone. Besides reliability, low cost, and an easy-to-handle procedure, an ability to recognize humulone as the major contributor of hop AO activity could allow DC polarographic assay to be applied in analysis of various hop-derived products.
TY - JOUR
AU - Gorjanović, Stanislava Z
AU - Pastor, Ferenc
AU - Vasic, Radica
AU - Novaković, Miroslav M
AU - Simonović, Mladen
AU - Milić Komić, Sonja
AU - Sužnjević, Desanka
PY - 2013
UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/682
AB - Antioxidant (AO) activity of extracts of hop cones (Serbian domestic varieties) and commercial hop products (Saaz, Spalter, Spalter select, and Magnum pellets) was determined by parallel application of recently developed direct current (DC) polarographic and widely used DPPH assay. Correlations between 2,2-diphenyl-1-picrylhydrazyl radical (DPPH) scavenging and total phenolics (TPC) determined by the Folin-Ciocalteu assay (FC) (0.99), and between H2O2 scavenging,: (HPS) and humulone content (H) determined by conductometric method (0.94), total resins (TR) (0.85), and hop storage index (HIS) (-0.90), were found statistically significant at p lt 0.05 level while complete lack of HPS correlation with TPC and DPPH was observed. To obtain an insight into differences between results of AO assays applied, activity of individual compounds, prevalent hop phenolics, and bitter acids was determined. By far superior HPS activity of humulone was followed by catechin, quercetin, xanthohumol, lupulone, and rutin. In contrast, DPPH scavenging activity of phenolics (quercetin > catechin > rutin > xantohumol) was found substantially higher than activity of bitter acids. According to ferric reducing antioxidant power (FRAP) and scavenging of 2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS), higher AO activity was ascribed to phenolics, while almost neglecting humulone. Besides reliability, low cost, and an easy-to-handle procedure, an ability to recognize humulone as the major contributor of hop AO activity could allow DC polarographic assay to be applied in analysis of various hop-derived products.
PB - Amer Chemical Soc, Washington
T2 - Journal of Agricultural and Food Chemistry
T1 - Electrochemical versus Spectrophotometric Assessment of Antioxidant Activity of Hop (Humulus lupulus L.) Products and Individual Compounds
EP - 9096
IS - 38
SP - 9089
VL - 61
DO - 10.1021/jf401718z
ER -
@article{
author = "Gorjanović, Stanislava Z and Pastor, Ferenc and Vasic, Radica and Novaković, Miroslav M and Simonović, Mladen and Milić Komić, Sonja and Sužnjević, Desanka",
year = "2013",
abstract = "Antioxidant (AO) activity of extracts of hop cones (Serbian domestic varieties) and commercial hop products (Saaz, Spalter, Spalter select, and Magnum pellets) was determined by parallel application of recently developed direct current (DC) polarographic and widely used DPPH assay. Correlations between 2,2-diphenyl-1-picrylhydrazyl radical (DPPH) scavenging and total phenolics (TPC) determined by the Folin-Ciocalteu assay (FC) (0.99), and between H2O2 scavenging,: (HPS) and humulone content (H) determined by conductometric method (0.94), total resins (TR) (0.85), and hop storage index (HIS) (-0.90), were found statistically significant at p lt 0.05 level while complete lack of HPS correlation with TPC and DPPH was observed. To obtain an insight into differences between results of AO assays applied, activity of individual compounds, prevalent hop phenolics, and bitter acids was determined. By far superior HPS activity of humulone was followed by catechin, quercetin, xanthohumol, lupulone, and rutin. In contrast, DPPH scavenging activity of phenolics (quercetin > catechin > rutin > xantohumol) was found substantially higher than activity of bitter acids. According to ferric reducing antioxidant power (FRAP) and scavenging of 2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS), higher AO activity was ascribed to phenolics, while almost neglecting humulone. Besides reliability, low cost, and an easy-to-handle procedure, an ability to recognize humulone as the major contributor of hop AO activity could allow DC polarographic assay to be applied in analysis of various hop-derived products.",
publisher = "Amer Chemical Soc, Washington",
journal = "Journal of Agricultural and Food Chemistry",
title = "Electrochemical versus Spectrophotometric Assessment of Antioxidant Activity of Hop (Humulus lupulus L.) Products and Individual Compounds",
pages = "9096-9089",
number = "38",
volume = "61",
doi = "10.1021/jf401718z"
}
Gorjanović, S. Z., Pastor, F., Vasic, R., Novaković, M. M., Simonović, M., Milić Komić, S.,& Sužnjević, D.. (2013). Electrochemical versus Spectrophotometric Assessment of Antioxidant Activity of Hop (Humulus lupulus L.) Products and Individual Compounds. in Journal of Agricultural and Food Chemistry
Amer Chemical Soc, Washington., 61(38), 9089-9096.
https://doi.org/10.1021/jf401718z
Gorjanović SZ, Pastor F, Vasic R, Novaković MM, Simonović M, Milić Komić S, Sužnjević D. Electrochemical versus Spectrophotometric Assessment of Antioxidant Activity of Hop (Humulus lupulus L.) Products and Individual Compounds. in Journal of Agricultural and Food Chemistry. 2013;61(38):9089-9096.
doi:10.1021/jf401718z .
Gorjanović, Stanislava Z, Pastor, Ferenc, Vasic, Radica, Novaković, Miroslav M, Simonović, Mladen, Milić Komić, Sonja, Sužnjević, Desanka, "Electrochemical versus Spectrophotometric Assessment of Antioxidant Activity of Hop (Humulus lupulus L.) Products and Individual Compounds" in Journal of Agricultural and Food Chemistry, 61, no. 38 (2013):9089-9096,
https://doi.org/10.1021/jf401718z . .
