Component analysis of fluorescence spectra of thiol DAB dendrimer/ZnSe-PEA nanoparticles
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2013
Authors
Algarra, ManuelRadotić, Ksenija
Kalauzi, Aleksandar
Alonso, Beatriz
Casado, CM
Esteves da Silva, Joaquim C.G.
Article (Published version)
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Show full item recordAbstract
The fluorescence spectroscopy technique is an accurate method and has great utility in the interpretation of complex systems based on several emission bands. An interpretation of the system requires determination of the number, positions and intensities of the spectral components. In this work, the emission spectra of the synthesized ZnSe complex coated with O-phosphorylethanolamine (ZnSe-PEA), both with and without thiol DAB dendrimer generation 5 (S-DAB G(5)), were analyzed using a combination of asymmetric (log-normal) and symmetric (Gaussian) models. The method applied for the deconvolution of fluorescence spectra has proven to be very sensitive for observing the stability of the ZnSe-PEA complex after binding with S-DAB. The ZnSe-PEA emission spectrum contains two components. The positions of the emission maxima of these two components are not significantly affected by the presence of S-DAB G(5) in the complex, which revealed the presence of a stable complex at a pH of 7. By apply...ing the spectral deconvolution method, strong evidence was obtained that suggested that the ZnSe-PEA complex is stable after complexation with S-DAB G5.
Keywords:
ZnSe Quantum dots / O-phosphorylethanolamine / Non-linear fitting / Log-normal model / DendrimersSource:
Talanta, 2013, 105, 267-271Publisher:
- Elsevier Science Bv, Amsterdam
Funding / projects:
- FCT (Lisbon, Portugal)Portuguese Foundation for Science and Technology [PTDC/QUI/71001/2006]
- Study of structure-function relationships in the plant cell wall and modifications of the wall structure by enzyme engineering (RS-MESTD-Basic Research (BR or ON)-173017)
- Spanish Ministerio de Ciencia e InnovacionInstituto de Salud Carlos IIISpanish Government [CTQ2009-12332-C02-01]
DOI: 10.1016/j.talanta.2012.09.008
ISSN: 0039-9140
PubMed: 23598018
WoS: 000319088500040
Scopus: 2-s2.0-84897658303
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Institution/Community
Institut za multidisciplinarna istraživanjaTY - JOUR AU - Algarra, Manuel AU - Radotić, Ksenija AU - Kalauzi, Aleksandar AU - Alonso, Beatriz AU - Casado, CM AU - Esteves da Silva, Joaquim C.G. PY - 2013 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/652 AB - The fluorescence spectroscopy technique is an accurate method and has great utility in the interpretation of complex systems based on several emission bands. An interpretation of the system requires determination of the number, positions and intensities of the spectral components. In this work, the emission spectra of the synthesized ZnSe complex coated with O-phosphorylethanolamine (ZnSe-PEA), both with and without thiol DAB dendrimer generation 5 (S-DAB G(5)), were analyzed using a combination of asymmetric (log-normal) and symmetric (Gaussian) models. The method applied for the deconvolution of fluorescence spectra has proven to be very sensitive for observing the stability of the ZnSe-PEA complex after binding with S-DAB. The ZnSe-PEA emission spectrum contains two components. The positions of the emission maxima of these two components are not significantly affected by the presence of S-DAB G(5) in the complex, which revealed the presence of a stable complex at a pH of 7. By applying the spectral deconvolution method, strong evidence was obtained that suggested that the ZnSe-PEA complex is stable after complexation with S-DAB G5. PB - Elsevier Science Bv, Amsterdam T2 - Talanta T1 - Component analysis of fluorescence spectra of thiol DAB dendrimer/ZnSe-PEA nanoparticles EP - 271 SP - 267 VL - 105 DO - 10.1016/j.talanta.2012.09.008 ER -
@article{ author = "Algarra, Manuel and Radotić, Ksenija and Kalauzi, Aleksandar and Alonso, Beatriz and Casado, CM and Esteves da Silva, Joaquim C.G.", year = "2013", abstract = "The fluorescence spectroscopy technique is an accurate method and has great utility in the interpretation of complex systems based on several emission bands. An interpretation of the system requires determination of the number, positions and intensities of the spectral components. In this work, the emission spectra of the synthesized ZnSe complex coated with O-phosphorylethanolamine (ZnSe-PEA), both with and without thiol DAB dendrimer generation 5 (S-DAB G(5)), were analyzed using a combination of asymmetric (log-normal) and symmetric (Gaussian) models. The method applied for the deconvolution of fluorescence spectra has proven to be very sensitive for observing the stability of the ZnSe-PEA complex after binding with S-DAB. The ZnSe-PEA emission spectrum contains two components. The positions of the emission maxima of these two components are not significantly affected by the presence of S-DAB G(5) in the complex, which revealed the presence of a stable complex at a pH of 7. By applying the spectral deconvolution method, strong evidence was obtained that suggested that the ZnSe-PEA complex is stable after complexation with S-DAB G5.", publisher = "Elsevier Science Bv, Amsterdam", journal = "Talanta", title = "Component analysis of fluorescence spectra of thiol DAB dendrimer/ZnSe-PEA nanoparticles", pages = "271-267", volume = "105", doi = "10.1016/j.talanta.2012.09.008" }
Algarra, M., Radotić, K., Kalauzi, A., Alonso, B., Casado, C.,& Esteves da Silva, J. C.G.. (2013). Component analysis of fluorescence spectra of thiol DAB dendrimer/ZnSe-PEA nanoparticles. in Talanta Elsevier Science Bv, Amsterdam., 105, 267-271. https://doi.org/10.1016/j.talanta.2012.09.008
Algarra M, Radotić K, Kalauzi A, Alonso B, Casado C, Esteves da Silva JC. Component analysis of fluorescence spectra of thiol DAB dendrimer/ZnSe-PEA nanoparticles. in Talanta. 2013;105:267-271. doi:10.1016/j.talanta.2012.09.008 .
Algarra, Manuel, Radotić, Ksenija, Kalauzi, Aleksandar, Alonso, Beatriz, Casado, CM, Esteves da Silva, Joaquim C.G., "Component analysis of fluorescence spectra of thiol DAB dendrimer/ZnSe-PEA nanoparticles" in Talanta, 105 (2013):267-271, https://doi.org/10.1016/j.talanta.2012.09.008 . .