The interactions of vanadium with Phycomyces blakesleeanus mycelium: enzymatic reduction, transport and metabolic effects
Само за регистроване кориснике
2013
Аутори
Žižić, MilanZivić, Miroslav
Spasojević, Ivan
Bogdanović Pristov, Jelena
Stanić, Marina
Cvetić-Antić, Tijana
Zakrzewska, Joanna
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The biological and chemical basis of vanadium action and transport in fungi is relatively poorly understood. In this study we investigated the interactions of vanadium in physiologically-relevant redox states: vanadate (+5) and vanadyl (+4), with mycelium of fungus Phycomyces blakesleeanus using EPR and P-31 NMR spectroscopy and biochemical assays. We determined that P. blakesleeanus reduces V5+ to V4+ in the extracellular compartment by the means of cell surface enzyme with ferricyanide reductase activity, which contains molybdenum molybdopterin as a cofactor. Both, V5+ and V4+ bind to cell wall. They enter the cytoplasm via phosphate transporter and cation channels, respectively, and exhibit different metabolic effects. Vanadate provokes increased biomass production, the effects being inverted to toxic at higher V5+ concentrations. In addition, V5+ activates the synthesis of sugar phosphates and oligophosphates. On the other hand, V4+ exhibits toxic effects even at low concentrations.... The V4+ detoxification route involves binding to vacuolar polyphosphates. Altogether our results imply that the mechanism of interaction of vanadium with P. blakesleeanus involves three major steps: extracellular enzymatic V5+/V4+ reduction, V4+ influx, and vacuolar storage, with an additional step -V5+ import occurring at higher vanadate concentrations.
Кључне речи:
Vanadyl / Vanadate / Reductase / Phycomyces blakesleeanus / P-31 NMR / EPRИзвор:
Research in Microbiology, 2013, 164, 1, 61-69Издавач:
- Elsevier Science Bv, Amsterdam
Финансирање / пројекти:
- Интеракције мембрана са унутарћелијским и апопластичним простором: изучавања биоенергетике и сингализације користећи биофизичке и биохемијске методе (RS-MESTD-Basic Research (BR or ON)-173040)
- Испитивања односа структура-функција у ћелијском зиду биљака и измене структуре зида ензимским инжењерингом (RS-MESTD-Basic Research (BR or ON)-173017)
DOI: 10.1016/j.resmic.2012.08.007
ISSN: 0923-2508
PubMed: 22992386
WoS: 000313542400010
Scopus: 2-s2.0-84871722584
Институција/група
Institut za multidisciplinarna istraživanjaTY - JOUR AU - Žižić, Milan AU - Zivić, Miroslav AU - Spasojević, Ivan AU - Bogdanović Pristov, Jelena AU - Stanić, Marina AU - Cvetić-Antić, Tijana AU - Zakrzewska, Joanna PY - 2013 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/632 AB - The biological and chemical basis of vanadium action and transport in fungi is relatively poorly understood. In this study we investigated the interactions of vanadium in physiologically-relevant redox states: vanadate (+5) and vanadyl (+4), with mycelium of fungus Phycomyces blakesleeanus using EPR and P-31 NMR spectroscopy and biochemical assays. We determined that P. blakesleeanus reduces V5+ to V4+ in the extracellular compartment by the means of cell surface enzyme with ferricyanide reductase activity, which contains molybdenum molybdopterin as a cofactor. Both, V5+ and V4+ bind to cell wall. They enter the cytoplasm via phosphate transporter and cation channels, respectively, and exhibit different metabolic effects. Vanadate provokes increased biomass production, the effects being inverted to toxic at higher V5+ concentrations. In addition, V5+ activates the synthesis of sugar phosphates and oligophosphates. On the other hand, V4+ exhibits toxic effects even at low concentrations. The V4+ detoxification route involves binding to vacuolar polyphosphates. Altogether our results imply that the mechanism of interaction of vanadium with P. blakesleeanus involves three major steps: extracellular enzymatic V5+/V4+ reduction, V4+ influx, and vacuolar storage, with an additional step -V5+ import occurring at higher vanadate concentrations. PB - Elsevier Science Bv, Amsterdam T2 - Research in Microbiology T1 - The interactions of vanadium with Phycomyces blakesleeanus mycelium: enzymatic reduction, transport and metabolic effects EP - 69 IS - 1 SP - 61 VL - 164 DO - 10.1016/j.resmic.2012.08.007 ER -
@article{ author = "Žižić, Milan and Zivić, Miroslav and Spasojević, Ivan and Bogdanović Pristov, Jelena and Stanić, Marina and Cvetić-Antić, Tijana and Zakrzewska, Joanna", year = "2013", abstract = "The biological and chemical basis of vanadium action and transport in fungi is relatively poorly understood. In this study we investigated the interactions of vanadium in physiologically-relevant redox states: vanadate (+5) and vanadyl (+4), with mycelium of fungus Phycomyces blakesleeanus using EPR and P-31 NMR spectroscopy and biochemical assays. We determined that P. blakesleeanus reduces V5+ to V4+ in the extracellular compartment by the means of cell surface enzyme with ferricyanide reductase activity, which contains molybdenum molybdopterin as a cofactor. Both, V5+ and V4+ bind to cell wall. They enter the cytoplasm via phosphate transporter and cation channels, respectively, and exhibit different metabolic effects. Vanadate provokes increased biomass production, the effects being inverted to toxic at higher V5+ concentrations. In addition, V5+ activates the synthesis of sugar phosphates and oligophosphates. On the other hand, V4+ exhibits toxic effects even at low concentrations. The V4+ detoxification route involves binding to vacuolar polyphosphates. Altogether our results imply that the mechanism of interaction of vanadium with P. blakesleeanus involves three major steps: extracellular enzymatic V5+/V4+ reduction, V4+ influx, and vacuolar storage, with an additional step -V5+ import occurring at higher vanadate concentrations.", publisher = "Elsevier Science Bv, Amsterdam", journal = "Research in Microbiology", title = "The interactions of vanadium with Phycomyces blakesleeanus mycelium: enzymatic reduction, transport and metabolic effects", pages = "69-61", number = "1", volume = "164", doi = "10.1016/j.resmic.2012.08.007" }
Žižić, M., Zivić, M., Spasojević, I., Bogdanović Pristov, J., Stanić, M., Cvetić-Antić, T.,& Zakrzewska, J.. (2013). The interactions of vanadium with Phycomyces blakesleeanus mycelium: enzymatic reduction, transport and metabolic effects. in Research in Microbiology Elsevier Science Bv, Amsterdam., 164(1), 61-69. https://doi.org/10.1016/j.resmic.2012.08.007
Žižić M, Zivić M, Spasojević I, Bogdanović Pristov J, Stanić M, Cvetić-Antić T, Zakrzewska J. The interactions of vanadium with Phycomyces blakesleeanus mycelium: enzymatic reduction, transport and metabolic effects. in Research in Microbiology. 2013;164(1):61-69. doi:10.1016/j.resmic.2012.08.007 .
Žižić, Milan, Zivić, Miroslav, Spasojević, Ivan, Bogdanović Pristov, Jelena, Stanić, Marina, Cvetić-Antić, Tijana, Zakrzewska, Joanna, "The interactions of vanadium with Phycomyces blakesleeanus mycelium: enzymatic reduction, transport and metabolic effects" in Research in Microbiology, 164, no. 1 (2013):61-69, https://doi.org/10.1016/j.resmic.2012.08.007 . .