Biochemical methods available for the measurement of antioxidant activity in salt-stressed tissues are reviewed, outlining the most important advantages and shortcomings of the methods. Here we consider commonly used methods for measuring total antioxidant capacity and phenolic content, ABTS and Folin-Ciocalteu's procedure, respectively. Moreover, we presented assays for determination of antioxidant enzymes activities: superoxide dismutase, catalase, and ascorbate peroxidase. This choice of methods enables us to elucidate a full profile of antioxidant activities, evaluating their effectiveness against various reactive oxygen species produced during salt stress.
Keywords:
Total phenolic content / Total antioxidant capacity / Superoxide dismutase / Salinity / Protein concentration / Catalase / Ascorbate peroxidase
TY - JOUR
AU - Dragišić Maksimović, Jelena
AU - Živanović, Branka D.
PY - 2012
UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/612
AB - Biochemical methods available for the measurement of antioxidant activity in salt-stressed tissues are reviewed, outlining the most important advantages and shortcomings of the methods. Here we consider commonly used methods for measuring total antioxidant capacity and phenolic content, ABTS and Folin-Ciocalteu's procedure, respectively. Moreover, we presented assays for determination of antioxidant enzymes activities: superoxide dismutase, catalase, and ascorbate peroxidase. This choice of methods enables us to elucidate a full profile of antioxidant activities, evaluating their effectiveness against various reactive oxygen species produced during salt stress.
PB - Humana Press Inc.
T2 - Methods in Molecular Biology
T1 - Quantification of the antioxidant activity in salt-stressed tissues
EP - 250
SP - 237
VL - 913
DO - 10.1007/978-1-61779-986-0_16
ER -
@article{
author = "Dragišić Maksimović, Jelena and Živanović, Branka D.",
year = "2012",
abstract = "Biochemical methods available for the measurement of antioxidant activity in salt-stressed tissues are reviewed, outlining the most important advantages and shortcomings of the methods. Here we consider commonly used methods for measuring total antioxidant capacity and phenolic content, ABTS and Folin-Ciocalteu's procedure, respectively. Moreover, we presented assays for determination of antioxidant enzymes activities: superoxide dismutase, catalase, and ascorbate peroxidase. This choice of methods enables us to elucidate a full profile of antioxidant activities, evaluating their effectiveness against various reactive oxygen species produced during salt stress.",
publisher = "Humana Press Inc.",
journal = "Methods in Molecular Biology",
title = "Quantification of the antioxidant activity in salt-stressed tissues",
pages = "250-237",
volume = "913",
doi = "10.1007/978-1-61779-986-0_16"
}
Dragišić Maksimović, J.,& Živanović, Branka D.. (2012). Quantification of the antioxidant activity in salt-stressed tissues. in Methods in Molecular Biology
Humana Press Inc.., 913, 237-250.
https://doi.org/10.1007/978-1-61779-986-0_16
Dragišić Maksimović J, Živanović, Branka D.. Quantification of the antioxidant activity in salt-stressed tissues. in Methods in Molecular Biology. 2012;913:237-250.
doi:10.1007/978-1-61779-986-0_16 .
Dragišić Maksimović, Jelena, Živanović, Branka D., "Quantification of the antioxidant activity in salt-stressed tissues" in Methods in Molecular Biology, 913 (2012):237-250,
https://doi.org/10.1007/978-1-61779-986-0_16 . .
