Приказ основних података о документу

dc.creatorMitic, Nevena V
dc.creatorDmitrović, Slavica
dc.creatorĐorđević, Mirka
dc.creatorZdravkovic-Korac, Snežana R
dc.creatorNikolic, Radomirka R
dc.creatorRaspor, Martin
dc.creatorĐorđević, Tatjana
dc.creatorMaksimović, Vuk
dc.creatorŽivković, Suzana
dc.creatorKrstić-Milošević, Dijana B.
dc.creatorStanisic, Mariana
dc.creatorNinković, Slavica
dc.date.accessioned2022-04-05T14:38:22Z
dc.date.available2022-04-05T14:38:22Z
dc.date.issued2012
dc.identifier.issn0176-1617
dc.identifier.urihttp://rimsi.imsi.bg.ac.rs/handle/123456789/599
dc.description.abstractWe investigated Chenopodium murale transgenic hairy root in vitro culture system as a new tool for allelopathic assays. Transgenic hairy roots were induced by Agrobacterium rhizogenes A4M70GUS from roots, cotyledons, leaves, and internodes of C. murale seedlings. Roots were found to be the best target explants, providing transformation efficiency of up to 11.1%. Established hairy root clones differed in their morphology and growth potential. Molecular characterization of these clones was carried out by PCR, RT-PCR and histochemical GUS analyses. No differences in rol gene expression were observed. Liquid culture system of characterized hairy root clones was maintained for over 2 years. Six hairy root clones were selected for assaying the allelopathic effect of their growth medium against germination and seedling elongation of wheat and lettuce test plants. The inhibitory potential varied depending on the hairy root clone. Some transgenic clones showed significantly higher inhibition compared to wild-type roots. These results revealed that hairy roots as an independent system synthesize some bioactive substances with allelopathic activity and exude them into the growth medium. Concentrations of caffeic, ferulic and p-coumaric acids (0.07-2.85 mu mol/L) identified by HPLC analysis in the growth media were at least 1000 times lower than the inhibitory active concentration (5 mmol/L) of pure grade phenolic acids, suggesting that they have a limited role in the allelopathic phenomena of C murale. The presented hairy root system appears to be a suitable tool for further investigation of the potential and nature of root-mediated allelopathic interference of C. murale.en
dc.publisherElsevier Gmbh, Munich
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173015/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173024/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173040/RS//
dc.rightsrestrictedAccess
dc.sourceJournal of Plant Physiology
dc.subjectRol genesen
dc.subjectPhenolic acidsen
dc.subjectHairy rootsen
dc.subjectGenetic transformationen
dc.subjectChenopodium muraleen
dc.subjectAllelopathyen
dc.subjectAgrobacterium rhizogenesen
dc.titleUse of Chenopodium murale L. transgenic hairy root in vitro culture system as a new tool for allelopathic assaysen
dc.typearticle
dc.rights.licenseARR
dc.citation.epage1211
dc.citation.issue12
dc.citation.other169(12): 1203-1211
dc.citation.rankM21
dc.citation.spage1203
dc.citation.volume169
dc.identifier.doi10.1016/j.jplph.2012.04.009
dc.identifier.pmid22749286
dc.identifier.scopus2-s2.0-84863880682
dc.identifier.wos000310392900010
dc.type.versionpublishedVersion


Документи

Thumbnail

Овај документ се појављује у следећим колекцијама

Приказ основних података о документу