Приказ основних података о документу

dc.creatorŽivanović, Branka D.
dc.date.accessioned2022-04-05T14:37:41Z
dc.date.available2022-04-05T14:37:41Z
dc.date.issued2012
dc.identifier.issn0032-0935
dc.identifier.urihttp://rimsi.imsi.bg.ac.rs/handle/123456789/588
dc.description.abstractNet fluxes of Ca2+ and H+ ions were measured non-invasively close to the surface of Phycomyces blakesleeanus sporangiophores stage I using ion-selective vibrating microelectrodes. The measurements were performed on a wild type (Wt) and a gravitropic mutant A909 kept in either vertical or tilted orientation. Microelectrodes were positioned 4 mu m from the surface of sporangiophore, and ion fluxes were recorded from the apical (0-20 mu m) and subapical (50-100 mu m) regions. The magnitude and direction of ionic fluxes measured were dependent on the distance from the tip along the growing zone of sporangiophore. Vertically oriented sporangiophores displayed characteristic tip-to-base ion fluxes patterns. Ca2+ and H+ fluxes recorded from apical region of Wt sporangiophores were inward-directed, while ion fluxes from subapical locations occurred in both directions. In contrast to Wt, mutant A909 showed opposite (outward) direction of Ca2+ fluxes and reduced H+ influxes in the apical region. Following gravistimulation, the magnitude and direction of ionic fluxes were altered. Wt sporangiophore exhibited oppositely directed fluxes on the lower (influx) and the upper (efflux) sides of the cell, while mutant A909 did not show such patterns. A variable elongation growth in vertical position and reduced growth rate upon gravistimulation were observed in both strains. The data show that tip-growing sporangiophores exhibit a tip-to-base ion flux pattern which changes characteristically upon gravistimulation in Wt in contrast to the mutant A909 with a strongly reduced gravitropic response.en
dc.publisherSpringer, New York
dc.relationDAADDeutscher Akademischer Austausch Dienst (DAAD)European Commission
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173040/RS//
dc.rightsrestrictedAccess
dc.sourcePlanta
dc.subjectTip growthen
dc.subjectIon-selective microelectrodesen
dc.subjectIon fluxesen
dc.subjectGrowth rateen
dc.subjectGravitropic bendingen
dc.titleSurface tip-to-base Ca2+ and H+ ionic fluxes are involved in apical growth and graviperception of the Phycomyces stage I sporangiophoreen
dc.typearticle
dc.rights.licenseARR
dc.citation.epage1829
dc.citation.issue6
dc.citation.other236(6): 1817-1829
dc.citation.rankM21
dc.citation.spage1817
dc.citation.volume236
dc.identifier.doi10.1007/s00425-012-1738-3
dc.identifier.pmid22910875
dc.identifier.scopus2-s2.0-84869506020
dc.identifier.wos000311398100012
dc.type.versionpublishedVersion


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Приказ основних података о документу