Sugars and acid invertase mediate the physiological response of Schenkia spicata root cultures to salt stress
Само за регистроване кориснике
2012
Аутори
Misic, DanijelaDragicević, Milan
Siler, Branislav
Nestorović-Živković, Jasmina M
Maksimović, Vuk
Momcilović, Ivana
Nikolic, Miroslav
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
A heterotrophic model system was established in our studies in order to differentiate the effect of high salt concentrations in external medium on growth and sugar metabolism in roots from the effect of reduced sugar availability resulting from decreased photosynthesis under salinity. Soluble sugar content and the activity of acid invertase in root cultures of salt-tolerant (ST) and salt-sensitive (SS) Schenkia spicata (L.) Mansion genotypes were investigated during exposure to different NaCI concentrations (0-200 mM). Their response to severe salinity was characterized by a metabolic adjustment that led to the accumulation of sucrose (Suc) in root tissues. There was clear evidence that cell wall invertase (CW-Inv) is, the major contributor to the Suc/hexose ratio in roots during exposure to elevated salinity. The results of CW-Inv activity and immunodetection assays in our study suggest that the regulation of CW-Inv expression is most likely achieved in a salt stress dependent manner.... Also, NaCI modulated soluble acid invertase (SA-Inv) expression differentially in SS and ST genotypes of S. spicata. Regardless of the salt treatment, genotype, or the amount of enzyme, SA-Inv activity was generally low, indicating regulation at the posttranslational level. The results suggest no direct role of SA-Inv in the regulation of the root tissue carbohydrate pool and therefore in the control of the availability of glucose and fructose for the primary metabolism and/or osmotic adjustment in the present heterotrophic model system, 2012 Elsevier GmbH. All rights reserved.
Кључне речи:
Sugar uptake / Soluble sugars / Salt stress / Root culture / Acid invertaseИзвор:
Journal of Plant Physiology, 2012, 169, 13, 1281-1289Издавач:
- Elsevier Gmbh, Urban & Fischer Verlag, Jena
Финансирање / пројекти:
- Физиолошка, хемијска и молекуларна анализа диверзитета одабраних ретких и угрожених биљних врста у циљу еx ситу заштите и продукције биолошки активних једињења (RS-173024)
- Минерални стрес и адаптације биљака на маргиналним пољопривредним земљиштима (RS-173028)
- Интеракције мембрана са унутарћелијским и апопластичним простором: изучавања биоенергетике и сингализације користећи биофизичке и биохемијске методе (RS-173040)
DOI: 10.1016/j.jplph.2012.04.018
ISSN: 0176-1617
PubMed: 22795677
WoS: 000309246700007
Scopus: 2-s2.0-84864945953
Институција/група
Institut za multidisciplinarna istraživanjaTY - JOUR AU - Misic, Danijela AU - Dragicević, Milan AU - Siler, Branislav AU - Nestorović-Živković, Jasmina M AU - Maksimović, Vuk AU - Momcilović, Ivana AU - Nikolic, Miroslav PY - 2012 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/542 AB - A heterotrophic model system was established in our studies in order to differentiate the effect of high salt concentrations in external medium on growth and sugar metabolism in roots from the effect of reduced sugar availability resulting from decreased photosynthesis under salinity. Soluble sugar content and the activity of acid invertase in root cultures of salt-tolerant (ST) and salt-sensitive (SS) Schenkia spicata (L.) Mansion genotypes were investigated during exposure to different NaCI concentrations (0-200 mM). Their response to severe salinity was characterized by a metabolic adjustment that led to the accumulation of sucrose (Suc) in root tissues. There was clear evidence that cell wall invertase (CW-Inv) is, the major contributor to the Suc/hexose ratio in roots during exposure to elevated salinity. The results of CW-Inv activity and immunodetection assays in our study suggest that the regulation of CW-Inv expression is most likely achieved in a salt stress dependent manner. Also, NaCI modulated soluble acid invertase (SA-Inv) expression differentially in SS and ST genotypes of S. spicata. Regardless of the salt treatment, genotype, or the amount of enzyme, SA-Inv activity was generally low, indicating regulation at the posttranslational level. The results suggest no direct role of SA-Inv in the regulation of the root tissue carbohydrate pool and therefore in the control of the availability of glucose and fructose for the primary metabolism and/or osmotic adjustment in the present heterotrophic model system, 2012 Elsevier GmbH. All rights reserved. PB - Elsevier Gmbh, Urban & Fischer Verlag, Jena T2 - Journal of Plant Physiology T1 - Sugars and acid invertase mediate the physiological response of Schenkia spicata root cultures to salt stress EP - 1289 IS - 13 SP - 1281 VL - 169 DO - 10.1016/j.jplph.2012.04.018 ER -
@article{ author = "Misic, Danijela and Dragicević, Milan and Siler, Branislav and Nestorović-Živković, Jasmina M and Maksimović, Vuk and Momcilović, Ivana and Nikolic, Miroslav", year = "2012", abstract = "A heterotrophic model system was established in our studies in order to differentiate the effect of high salt concentrations in external medium on growth and sugar metabolism in roots from the effect of reduced sugar availability resulting from decreased photosynthesis under salinity. Soluble sugar content and the activity of acid invertase in root cultures of salt-tolerant (ST) and salt-sensitive (SS) Schenkia spicata (L.) Mansion genotypes were investigated during exposure to different NaCI concentrations (0-200 mM). Their response to severe salinity was characterized by a metabolic adjustment that led to the accumulation of sucrose (Suc) in root tissues. There was clear evidence that cell wall invertase (CW-Inv) is, the major contributor to the Suc/hexose ratio in roots during exposure to elevated salinity. The results of CW-Inv activity and immunodetection assays in our study suggest that the regulation of CW-Inv expression is most likely achieved in a salt stress dependent manner. Also, NaCI modulated soluble acid invertase (SA-Inv) expression differentially in SS and ST genotypes of S. spicata. Regardless of the salt treatment, genotype, or the amount of enzyme, SA-Inv activity was generally low, indicating regulation at the posttranslational level. The results suggest no direct role of SA-Inv in the regulation of the root tissue carbohydrate pool and therefore in the control of the availability of glucose and fructose for the primary metabolism and/or osmotic adjustment in the present heterotrophic model system, 2012 Elsevier GmbH. All rights reserved.", publisher = "Elsevier Gmbh, Urban & Fischer Verlag, Jena", journal = "Journal of Plant Physiology", title = "Sugars and acid invertase mediate the physiological response of Schenkia spicata root cultures to salt stress", pages = "1289-1281", number = "13", volume = "169", doi = "10.1016/j.jplph.2012.04.018" }
Misic, D., Dragicević, M., Siler, B., Nestorović-Živković, J. M., Maksimović, V., Momcilović, I.,& Nikolic, M.. (2012). Sugars and acid invertase mediate the physiological response of Schenkia spicata root cultures to salt stress. in Journal of Plant Physiology Elsevier Gmbh, Urban & Fischer Verlag, Jena., 169(13), 1281-1289. https://doi.org/10.1016/j.jplph.2012.04.018
Misic D, Dragicević M, Siler B, Nestorović-Živković JM, Maksimović V, Momcilović I, Nikolic M. Sugars and acid invertase mediate the physiological response of Schenkia spicata root cultures to salt stress. in Journal of Plant Physiology. 2012;169(13):1281-1289. doi:10.1016/j.jplph.2012.04.018 .
Misic, Danijela, Dragicević, Milan, Siler, Branislav, Nestorović-Živković, Jasmina M, Maksimović, Vuk, Momcilović, Ivana, Nikolic, Miroslav, "Sugars and acid invertase mediate the physiological response of Schenkia spicata root cultures to salt stress" in Journal of Plant Physiology, 169, no. 13 (2012):1281-1289, https://doi.org/10.1016/j.jplph.2012.04.018 . .