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Development and validation of a fluorometric method for the determination of hesperidin in human plasma and pharmaceutical forms
dc.creator | Pavun, Leposava A | |
dc.creator | Dimitrić-Marković, Jasmina | |
dc.creator | Djurdjević, Predrag T | |
dc.creator | Jelikic-Stankov, Milena D | |
dc.creator | Đikanović, Daniela | |
dc.creator | Ciric, Andrija | |
dc.creator | Malesev, Dusan L | |
dc.date.accessioned | 2022-04-05T14:32:38Z | |
dc.date.available | 2022-04-05T14:32:38Z | |
dc.date.issued | 2012 | |
dc.identifier.issn | 0352-5139 | |
dc.identifier.uri | http://rimsi.imsi.bg.ac.rs/handle/123456789/512 | |
dc.description.abstract | A fluorometric method, based on the fluorescence properties of the aluminium(III)-hesperidin complex, for the determination of hesperidin in human plasma and pharmaceutical forms has been developed and validated. The complex shows a strong emission in the presence of the surfactant betain sulphonate SB 12 at 476 nm with excitation at 390 nm. The linearity range for pharmaceutical forms of hesperidin was 0.06-24.4 mu g mL(-1) with a limit of detection, LOD, of 0.016 mu g mL(-1) and a limit of quantification, LOQ, of 0.049 mu g mL(-1). Recovery values in the range 99.3-99.7 % indicate good accuracy of the method. A linear dependence of the intensity of fluorescence of the complex on the concentration of hesperidin in plasma was obtained in concentration range from 0.1-12.2 mu g mL(-1). The LOD was 0.032 mu g mL(-1) while LOQ was 0.096 mu g mL(-1). Recovery values were in the range 98.4-99.8 %. The reliability of the method was checked by an LC MS/MS method for plasma samples and an HPLC/UV method for tablets with direct determination of hesperidin after separation. Linearity range in determination of hesperidin in pharmaceutical forms was obtained in the range from 0.05 to 10.00 mu g mL(-1). The LOD was 0.01 mu g mL(-1) and the LOQ was 0.03 mu g mL(-1). The linearity range for the determination of hesperidin in plasma was 0.02-10.00 mu g mL(-1) with an LOD 0.005 mu g mL(-1) and an LOQ of 0.015 mu g mL(-1). The good agreement between the two methods indicates the usability of the proposed fluorometric method for the simple, precise and accurate determination of hesperidin in clinical and quality control laboratories. | en |
dc.publisher | Srpsko hemijsko društvo, Beograd | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172016/RS// | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173017/RS// | |
dc.rights | openAccess | |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/4.0/ | |
dc.source | Journal of the Serbian Chemical Society | |
dc.subject | tablets | en |
dc.subject | LC-MS/MS | en |
dc.subject | human plasma | en |
dc.subject | hesperidin | en |
dc.subject | fluorometry | en |
dc.title | Development and validation of a fluorometric method for the determination of hesperidin in human plasma and pharmaceutical forms | en |
dc.type | article | |
dc.rights.license | BY-NC-ND | |
dc.citation.epage | 1640 | |
dc.citation.issue | 11 | |
dc.citation.other | 77(11): 1625-1640 | |
dc.citation.rank | M23 | |
dc.citation.spage | 1625 | |
dc.citation.volume | 77 | |
dc.identifier.doi | 10.2298/JSC111005060P | |
dc.identifier.fulltext | http://rimsi.imsi.bg.ac.rs/bitstream/id/727/509.pdf | |
dc.identifier.scopus | 2-s2.0-84871823513 | |
dc.identifier.wos | 000312746700010 | |
dc.type.version | publishedVersion |