Anoxia induces increased activity of alternative oxidase in fungus Phycomyces blakesleeanus
Само за регистроване кориснике
2011
Остало (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Alongside classical, cytochrome respiratory pathway, Phycomyces
blakesleeanus possess alternative, cyanideresistant
respiration (CRR) facilitated by alternative oxidase
(AOX). In order to study role of oxygen in regulation of CRR,
the effects of cyanide on respiration of 24h old mycelia in
aerated (control), hypoxic and anoxic conditions were measured.
Mycelium was incubated in these conditions for 1.5h,
3h and 5h. After 1.5h, AOX activity was increased only in
specimens incubated in anoxic conditions (13.6%). After 3h,
increase in AOX activity was significant in both hypoxic and
anoxic specimens (18.9% and 18.8%, respectively), with
even greater increase after 5h, 20.7% for hypoxic and 23.3%
for specimens in anoxic conditions. Mycelia treated for 5h
was then oxygenated for 10 minutes. This induced decrease
in AOX activity of 17% in anoxic and even 23.9% in hypoxic
mycelia. AOX is recognized as one of the mechanisms for
maintaining low levels of reduced ubiquione which ...can
function in conditions in which cytochrome chain is disabled,
such as anoxia. This is in concordance with results obtained
on P. blakesleeanus, where AOX levels rise in hypoxic and
anoxic conditions and decrease close to control level shortly
after introduction of oxygen into the system.
Кључне речи:
Phycomyces blakesleeanus / cellular respiration / AOX / anoxiaИзвор:
European Biophysics Journal With Biophysics Letters, 2011, 40, 186-186Издавач:
- Springer, New York
Институција/група
Institut za multidisciplinarna istraživanjaTY - GEN AU - Stanić, Marina AU - Zivić, Miroslav AU - Hadzibrahimović, Mirzeta R AU - Žižić, Milan AU - Zakrzewska, Joanna PY - 2011 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/497 AB - Alongside classical, cytochrome respiratory pathway, Phycomyces blakesleeanus possess alternative, cyanideresistant respiration (CRR) facilitated by alternative oxidase (AOX). In order to study role of oxygen in regulation of CRR, the effects of cyanide on respiration of 24h old mycelia in aerated (control), hypoxic and anoxic conditions were measured. Mycelium was incubated in these conditions for 1.5h, 3h and 5h. After 1.5h, AOX activity was increased only in specimens incubated in anoxic conditions (13.6%). After 3h, increase in AOX activity was significant in both hypoxic and anoxic specimens (18.9% and 18.8%, respectively), with even greater increase after 5h, 20.7% for hypoxic and 23.3% for specimens in anoxic conditions. Mycelia treated for 5h was then oxygenated for 10 minutes. This induced decrease in AOX activity of 17% in anoxic and even 23.9% in hypoxic mycelia. AOX is recognized as one of the mechanisms for maintaining low levels of reduced ubiquione which can function in conditions in which cytochrome chain is disabled, such as anoxia. This is in concordance with results obtained on P. blakesleeanus, where AOX levels rise in hypoxic and anoxic conditions and decrease close to control level shortly after introduction of oxygen into the system. PB - Springer, New York T2 - European Biophysics Journal With Biophysics Letters T1 - Anoxia induces increased activity of alternative oxidase in fungus Phycomyces blakesleeanus EP - 186 SP - 186 VL - 40 UR - https://hdl.handle.net/21.15107/rcub_rimsi_497 ER -
@misc{ author = "Stanić, Marina and Zivić, Miroslav and Hadzibrahimović, Mirzeta R and Žižić, Milan and Zakrzewska, Joanna", year = "2011", abstract = "Alongside classical, cytochrome respiratory pathway, Phycomyces blakesleeanus possess alternative, cyanideresistant respiration (CRR) facilitated by alternative oxidase (AOX). In order to study role of oxygen in regulation of CRR, the effects of cyanide on respiration of 24h old mycelia in aerated (control), hypoxic and anoxic conditions were measured. Mycelium was incubated in these conditions for 1.5h, 3h and 5h. After 1.5h, AOX activity was increased only in specimens incubated in anoxic conditions (13.6%). After 3h, increase in AOX activity was significant in both hypoxic and anoxic specimens (18.9% and 18.8%, respectively), with even greater increase after 5h, 20.7% for hypoxic and 23.3% for specimens in anoxic conditions. Mycelia treated for 5h was then oxygenated for 10 minutes. This induced decrease in AOX activity of 17% in anoxic and even 23.9% in hypoxic mycelia. AOX is recognized as one of the mechanisms for maintaining low levels of reduced ubiquione which can function in conditions in which cytochrome chain is disabled, such as anoxia. This is in concordance with results obtained on P. blakesleeanus, where AOX levels rise in hypoxic and anoxic conditions and decrease close to control level shortly after introduction of oxygen into the system.", publisher = "Springer, New York", journal = "European Biophysics Journal With Biophysics Letters", title = "Anoxia induces increased activity of alternative oxidase in fungus Phycomyces blakesleeanus", pages = "186-186", volume = "40", url = "https://hdl.handle.net/21.15107/rcub_rimsi_497" }
Stanić, M., Zivić, M., Hadzibrahimović, M. R., Žižić, M.,& Zakrzewska, J.. (2011). Anoxia induces increased activity of alternative oxidase in fungus Phycomyces blakesleeanus. in European Biophysics Journal With Biophysics Letters Springer, New York., 40, 186-186. https://hdl.handle.net/21.15107/rcub_rimsi_497
Stanić M, Zivić M, Hadzibrahimović MR, Žižić M, Zakrzewska J. Anoxia induces increased activity of alternative oxidase in fungus Phycomyces blakesleeanus. in European Biophysics Journal With Biophysics Letters. 2011;40:186-186. https://hdl.handle.net/21.15107/rcub_rimsi_497 .
Stanić, Marina, Zivić, Miroslav, Hadzibrahimović, Mirzeta R, Žižić, Milan, Zakrzewska, Joanna, "Anoxia induces increased activity of alternative oxidase in fungus Phycomyces blakesleeanus" in European Biophysics Journal With Biophysics Letters, 40 (2011):186-186, https://hdl.handle.net/21.15107/rcub_rimsi_497 .