The mechanism of iron (Fe) uptake from the leaf apoplast into leaf mesophyll cells was studied to evaluate the putative Fe inactivation as a possible cause of Fe deficiency chlorosis. For this purpose, sunflower (Helianthus annuus L.) and faba bean plants (Vicia faba L.) were precultured with varied Fe and bicarbonate (HCO3-) supply in nutrient solution. After 2-3 weeks preculture, Fe-III reduction and Fe-59 uptake by leaf discs were measured in solutions with Fe supplied as citrate or synthetic chelates in darkness. The data clearly indicate that Fe-III reduction is a prerequisite for Fe uptake into leaf cells and that the Fe nutritional status of plants does not affect either process. In addition, varied supply of Fe and HCO3- to the root medium during preculture had no effect on pH of the xylem sap and leaf apoplastic fluid. A varied pH of the incubation solution had no significant effect on Fe-III reduction and Fe uptake by leaf discs in the physiologically relevant pH range of 5.0...-6.0 as measured in the apoplastic leaf fluid. It is concluded that Fe inactivation in the leaf apoplast is not a primary cause of Fe deficiency chlorosis induced by bicarbonate.
Keywords:
Vicia faba L. / leaf apoplast / iron uptake / Helianthus annuus L. / Fe-III citrate reduction / Fe inactivation
TY - JOUR
AU - Nikolic, Miroslav
AU - Romheld, V
PY - 1999
UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/47
AB - The mechanism of iron (Fe) uptake from the leaf apoplast into leaf mesophyll cells was studied to evaluate the putative Fe inactivation as a possible cause of Fe deficiency chlorosis. For this purpose, sunflower (Helianthus annuus L.) and faba bean plants (Vicia faba L.) were precultured with varied Fe and bicarbonate (HCO3-) supply in nutrient solution. After 2-3 weeks preculture, Fe-III reduction and Fe-59 uptake by leaf discs were measured in solutions with Fe supplied as citrate or synthetic chelates in darkness. The data clearly indicate that Fe-III reduction is a prerequisite for Fe uptake into leaf cells and that the Fe nutritional status of plants does not affect either process. In addition, varied supply of Fe and HCO3- to the root medium during preculture had no effect on pH of the xylem sap and leaf apoplastic fluid. A varied pH of the incubation solution had no significant effect on Fe-III reduction and Fe uptake by leaf discs in the physiologically relevant pH range of 5.0-6.0 as measured in the apoplastic leaf fluid. It is concluded that Fe inactivation in the leaf apoplast is not a primary cause of Fe deficiency chlorosis induced by bicarbonate.
PB - Springer, Dordrecht
T2 - Plant and Soil
T1 - Mechanism of Fe uptake by the leaf symplast: Is Fe inactivation in leaf a cause of Fe deficiency chlorosis?
EP - 237
IS - 2
SP - 229
VL - 215
DO - 10.1023/A:1004786211779
ER -
@article{
author = "Nikolic, Miroslav and Romheld, V",
year = "1999",
abstract = "The mechanism of iron (Fe) uptake from the leaf apoplast into leaf mesophyll cells was studied to evaluate the putative Fe inactivation as a possible cause of Fe deficiency chlorosis. For this purpose, sunflower (Helianthus annuus L.) and faba bean plants (Vicia faba L.) were precultured with varied Fe and bicarbonate (HCO3-) supply in nutrient solution. After 2-3 weeks preculture, Fe-III reduction and Fe-59 uptake by leaf discs were measured in solutions with Fe supplied as citrate or synthetic chelates in darkness. The data clearly indicate that Fe-III reduction is a prerequisite for Fe uptake into leaf cells and that the Fe nutritional status of plants does not affect either process. In addition, varied supply of Fe and HCO3- to the root medium during preculture had no effect on pH of the xylem sap and leaf apoplastic fluid. A varied pH of the incubation solution had no significant effect on Fe-III reduction and Fe uptake by leaf discs in the physiologically relevant pH range of 5.0-6.0 as measured in the apoplastic leaf fluid. It is concluded that Fe inactivation in the leaf apoplast is not a primary cause of Fe deficiency chlorosis induced by bicarbonate.",
publisher = "Springer, Dordrecht",
journal = "Plant and Soil",
title = "Mechanism of Fe uptake by the leaf symplast: Is Fe inactivation in leaf a cause of Fe deficiency chlorosis?",
pages = "237-229",
number = "2",
volume = "215",
doi = "10.1023/A:1004786211779"
}
Nikolic, M.,& Romheld, V.. (1999). Mechanism of Fe uptake by the leaf symplast: Is Fe inactivation in leaf a cause of Fe deficiency chlorosis?. in Plant and Soil
Springer, Dordrecht., 215(2), 229-237.
https://doi.org/10.1023/A:1004786211779
Nikolic M, Romheld V. Mechanism of Fe uptake by the leaf symplast: Is Fe inactivation in leaf a cause of Fe deficiency chlorosis?. in Plant and Soil. 1999;215(2):229-237.
doi:10.1023/A:1004786211779 .
Nikolic, Miroslav, Romheld, V, "Mechanism of Fe uptake by the leaf symplast: Is Fe inactivation in leaf a cause of Fe deficiency chlorosis?" in Plant and Soil, 215, no. 2 (1999):229-237,
https://doi.org/10.1023/A:1004786211779 . .
