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dc.creatorDonaldson, Lloyd
dc.creatorRadotić, Ksenija
dc.creatorKalauzi, Aleksandar
dc.creatorĐikanović, Daniela
dc.creatorJeremic, Milorad G
dc.date.accessioned2022-04-05T14:26:26Z
dc.date.available2022-04-05T14:26:26Z
dc.date.issued2010
dc.identifier.issn1047-8477
dc.identifier.urihttp://rimsi.imsi.bg.ac.rs/handle/123456789/418
dc.description.abstractConfocal fluorescence microscopy was used to examine the spectral characteristics of lignin autofluorescence in secondary cell walls of normal and compression wood from Pinus radiata. Using UV excitation, fluorescence spectra of normal and compression wood sections showed significant differences, especially in the outer secondary cell wall of tracheids, with a shift in maxima from violet to blue wavelengths between normal and compression wood. A comparison of normal wood, mild and severe compression wood, showed that the wavelength shift was intermediate in the mild compression wood compared to the severe compression wood, thus offering the possibility of quantifying the severity by measuring ratios of fluorescence at violet and blue wavelengths. Fluorescence induced by blue light, rather than UV, was less well differentiated amongst wood types. Spectral deconvolution indicated the presence of a minimum of five discrete lignin fluorophores in the cell walls of both normal and compression wood tracheids. Comparison with lignin model compounds suggest that the wavelength shift may correspond in part to increased levels of p-hydroxy type lignin in the compression wood samples. The combination of confocal fluorescence imaging and related spectral deconvolution therefore offers a novel technique for characterising cell wall lignin in situ.en
dc.publisherAcademic Press Inc Elsevier Science, San Diego
dc.relationFoundation for Research, Science and Technology, New ZealandNew Zealand Foundation for Research, Science and Technology
dc.relationinfo:eu-repo/grantAgreement/MESTD/MPN2006-2010/143043/RS//
dc.rightsrestrictedAccess
dc.sourceJournal of Structural Biology
dc.subjectSpectral deconvolutionen
dc.subjectLigninen
dc.subjectFluorescenceen
dc.subjectCompression wooden
dc.subjectCell wallen
dc.titleQuantification of compression wood severity in tracheids of Pinus radiata D. Don using confocal fluorescence imaging and spectral deconvolutionen
dc.typearticle
dc.rights.licenseARR
dc.citation.epage115
dc.citation.issue1
dc.citation.other169(1): 106-115
dc.citation.rankM22
dc.citation.spage106
dc.citation.volume169
dc.identifier.doi10.1016/j.jsb.2009.09.006
dc.identifier.pmid19747548
dc.identifier.scopus2-s2.0-72749113020
dc.identifier.wos000274043100013
dc.type.versionpublishedVersion


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