Effects of genistein and daidzein on erythrocyte membrane fluidity: an electron paramagnetic resonance study
Нема приказа
Аутори
Ajdzanović, Vladimir ZSpasojević, Ivan
Filipović, Branko R
Sosic-Jurjević, Branka T
Sekulic, Milka I
Milošević, Verica Lj.
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The maintenance of erythrocyte membrane fluidity at the physiological level is an important factor affecting the ability of erythrocytes to pass through blood vessels of small luminal diameter. Genistein and daidzein, which are used as alternative therapeutics in cardiovascular conditions, can be incorporated into the cell membrane and change its fluidity. The aim of this study was to examine the effects of genistein and daidzein on erythrocyte membrane fluidity at graded depths. We used electron paramagnetic resonance (EPR) spectroscopy and fatty acid spin probes (5-DS and 12-DS) where EPR spectra were dependent on fluidity. The results showed that genistein significantly (p lt 0.05) decreased erythrocyte membrane fluidity near the hydrophilic surface, while daidzein significantly (p lt 0.05) increased the same parameter in deeper regions of the membrane. These data suggest that the deep fluidizing effects of daidzein on erythrocyte membranes make it a better therapeutic choice th...an genistein in some cardiovascular conditions.
Кључне речи:
spin probes / order parameter / membrane fluidity / genistein / erythrocytes / EPR / daidzeinИзвор:
Canadian Journal of Physiology and Pharmacology, 2010, 88, 4, 497-500Издавач:
- Natl Research Council Canada-N R C Research Press, Ottawa
Финансирање / пројекти:
- Утицај фитоестрогена, стероидних и пептидних хормона на ћелије неуроендокриног система (RS-143007)
- Биофизичка истраживања мембранских процеса: интеракција мембранских рецептора и канала са спољашњим факторима и интрацелуларна регулација (RS-143016)
DOI: 10.1139/Y10-020
ISSN: 0008-4212
PubMed: 20555419
WoS: 000279243700012
Scopus: 2-s2.0-77952068968
Институција/група
Institut za multidisciplinarna istraživanjaTY - JOUR AU - Ajdzanović, Vladimir Z AU - Spasojević, Ivan AU - Filipović, Branko R AU - Sosic-Jurjević, Branka T AU - Sekulic, Milka I AU - Milošević, Verica Lj. PY - 2010 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/414 AB - The maintenance of erythrocyte membrane fluidity at the physiological level is an important factor affecting the ability of erythrocytes to pass through blood vessels of small luminal diameter. Genistein and daidzein, which are used as alternative therapeutics in cardiovascular conditions, can be incorporated into the cell membrane and change its fluidity. The aim of this study was to examine the effects of genistein and daidzein on erythrocyte membrane fluidity at graded depths. We used electron paramagnetic resonance (EPR) spectroscopy and fatty acid spin probes (5-DS and 12-DS) where EPR spectra were dependent on fluidity. The results showed that genistein significantly (p lt 0.05) decreased erythrocyte membrane fluidity near the hydrophilic surface, while daidzein significantly (p lt 0.05) increased the same parameter in deeper regions of the membrane. These data suggest that the deep fluidizing effects of daidzein on erythrocyte membranes make it a better therapeutic choice than genistein in some cardiovascular conditions. PB - Natl Research Council Canada-N R C Research Press, Ottawa T2 - Canadian Journal of Physiology and Pharmacology T1 - Effects of genistein and daidzein on erythrocyte membrane fluidity: an electron paramagnetic resonance study EP - 500 IS - 4 SP - 497 VL - 88 DO - 10.1139/Y10-020 ER -
@article{ author = "Ajdzanović, Vladimir Z and Spasojević, Ivan and Filipović, Branko R and Sosic-Jurjević, Branka T and Sekulic, Milka I and Milošević, Verica Lj.", year = "2010", abstract = "The maintenance of erythrocyte membrane fluidity at the physiological level is an important factor affecting the ability of erythrocytes to pass through blood vessels of small luminal diameter. Genistein and daidzein, which are used as alternative therapeutics in cardiovascular conditions, can be incorporated into the cell membrane and change its fluidity. The aim of this study was to examine the effects of genistein and daidzein on erythrocyte membrane fluidity at graded depths. We used electron paramagnetic resonance (EPR) spectroscopy and fatty acid spin probes (5-DS and 12-DS) where EPR spectra were dependent on fluidity. The results showed that genistein significantly (p lt 0.05) decreased erythrocyte membrane fluidity near the hydrophilic surface, while daidzein significantly (p lt 0.05) increased the same parameter in deeper regions of the membrane. These data suggest that the deep fluidizing effects of daidzein on erythrocyte membranes make it a better therapeutic choice than genistein in some cardiovascular conditions.", publisher = "Natl Research Council Canada-N R C Research Press, Ottawa", journal = "Canadian Journal of Physiology and Pharmacology", title = "Effects of genistein and daidzein on erythrocyte membrane fluidity: an electron paramagnetic resonance study", pages = "500-497", number = "4", volume = "88", doi = "10.1139/Y10-020" }
Ajdzanović, V. Z., Spasojević, I., Filipović, B. R., Sosic-Jurjević, B. T., Sekulic, M. I.,& Milošević, V. Lj.. (2010). Effects of genistein and daidzein on erythrocyte membrane fluidity: an electron paramagnetic resonance study. in Canadian Journal of Physiology and Pharmacology Natl Research Council Canada-N R C Research Press, Ottawa., 88(4), 497-500. https://doi.org/10.1139/Y10-020
Ajdzanović VZ, Spasojević I, Filipović BR, Sosic-Jurjević BT, Sekulic MI, Milošević VL. Effects of genistein and daidzein on erythrocyte membrane fluidity: an electron paramagnetic resonance study. in Canadian Journal of Physiology and Pharmacology. 2010;88(4):497-500. doi:10.1139/Y10-020 .
Ajdzanović, Vladimir Z, Spasojević, Ivan, Filipović, Branko R, Sosic-Jurjević, Branka T, Sekulic, Milka I, Milošević, Verica Lj., "Effects of genistein and daidzein on erythrocyte membrane fluidity: an electron paramagnetic resonance study" in Canadian Journal of Physiology and Pharmacology, 88, no. 4 (2010):497-500, https://doi.org/10.1139/Y10-020 . .