The aim of the present study is to detect the monodehydroascorbic acid (MDA) radical in broad bean (Vicia faba L.) leaves which were treated by vacuum-infiltration in Na2SO3 solution and subsequent centrifugation (sulfite-treated leaves). When sulfite-treated leaves were illuminated with white light, the electron spin resonance (ESR) signal of MDA radical was observed. The level of the MDA radical depended on the concentration of sulfite that was used for vacuum-infiltration and on the light intensity of illumination. The formation of the MDA radical in sulfite-treated leaves was inhibited by DCMU or by replacement of air with N-2. Glycolaldehyde also inhibited the formation of MDA radical in sulfite-treated leaves. Catalase activity was decreased by the sulfite treatment without affecting significantly the activities of ascorbate peroxidase (AA-POX) and of peroxidase which preferentially oxidizes phenolics (PhOH-POX). From these results, we conclude that the formation of the MDA radic...al in sulfite-treated leaves is catalyzed by peroxidases using the H2O2 which is generated by photorespiration and the Mehler reaction.
TY - JOUR
AU - Veljović-Jovanović, Sonja
AU - Oniki, T
AU - Takahama, Umeo
PY - 1998
UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/40
AB - The aim of the present study is to detect the monodehydroascorbic acid (MDA) radical in broad bean (Vicia faba L.) leaves which were treated by vacuum-infiltration in Na2SO3 solution and subsequent centrifugation (sulfite-treated leaves). When sulfite-treated leaves were illuminated with white light, the electron spin resonance (ESR) signal of MDA radical was observed. The level of the MDA radical depended on the concentration of sulfite that was used for vacuum-infiltration and on the light intensity of illumination. The formation of the MDA radical in sulfite-treated leaves was inhibited by DCMU or by replacement of air with N-2. Glycolaldehyde also inhibited the formation of MDA radical in sulfite-treated leaves. Catalase activity was decreased by the sulfite treatment without affecting significantly the activities of ascorbate peroxidase (AA-POX) and of peroxidase which preferentially oxidizes phenolics (PhOH-POX). From these results, we conclude that the formation of the MDA radical in sulfite-treated leaves is catalyzed by peroxidases using the H2O2 which is generated by photorespiration and the Mehler reaction.
PB - Japanese Soc Plant Physiologists, Kyoto
T2 - Plant and Cell Physiology
T1 - Detection of monodehydroascorbic acid radical in sulfite-treated leaves and mechanism of its formation
EP - 1208
IS - 11
SP - 1203
VL - 39
DO - 10.1093/oxfordjournals.pcp.a029321
ER -
@article{
author = "Veljović-Jovanović, Sonja and Oniki, T and Takahama, Umeo",
year = "1998",
abstract = "The aim of the present study is to detect the monodehydroascorbic acid (MDA) radical in broad bean (Vicia faba L.) leaves which were treated by vacuum-infiltration in Na2SO3 solution and subsequent centrifugation (sulfite-treated leaves). When sulfite-treated leaves were illuminated with white light, the electron spin resonance (ESR) signal of MDA radical was observed. The level of the MDA radical depended on the concentration of sulfite that was used for vacuum-infiltration and on the light intensity of illumination. The formation of the MDA radical in sulfite-treated leaves was inhibited by DCMU or by replacement of air with N-2. Glycolaldehyde also inhibited the formation of MDA radical in sulfite-treated leaves. Catalase activity was decreased by the sulfite treatment without affecting significantly the activities of ascorbate peroxidase (AA-POX) and of peroxidase which preferentially oxidizes phenolics (PhOH-POX). From these results, we conclude that the formation of the MDA radical in sulfite-treated leaves is catalyzed by peroxidases using the H2O2 which is generated by photorespiration and the Mehler reaction.",
publisher = "Japanese Soc Plant Physiologists, Kyoto",
journal = "Plant and Cell Physiology",
title = "Detection of monodehydroascorbic acid radical in sulfite-treated leaves and mechanism of its formation",
pages = "1208-1203",
number = "11",
volume = "39",
doi = "10.1093/oxfordjournals.pcp.a029321"
}
Veljović-Jovanović, S., Oniki, T.,& Takahama, U.. (1998). Detection of monodehydroascorbic acid radical in sulfite-treated leaves and mechanism of its formation. in Plant and Cell Physiology
Japanese Soc Plant Physiologists, Kyoto., 39(11), 1203-1208.
https://doi.org/10.1093/oxfordjournals.pcp.a029321
Veljović-Jovanović S, Oniki T, Takahama U. Detection of monodehydroascorbic acid radical in sulfite-treated leaves and mechanism of its formation. in Plant and Cell Physiology. 1998;39(11):1203-1208.
doi:10.1093/oxfordjournals.pcp.a029321 .
Veljović-Jovanović, Sonja, Oniki, T, Takahama, Umeo, "Detection of monodehydroascorbic acid radical in sulfite-treated leaves and mechanism of its formation" in Plant and Cell Physiology, 39, no. 11 (1998):1203-1208,
https://doi.org/10.1093/oxfordjournals.pcp.a029321 . .
