Dehydration-related changes of peroxidase and polyphenol oxidase activity in fronds of the resurrection fern asplenium ceterach l.
2010
Аутори
Živković, SuzanaPopović, M
Dragišić Maksimović, Jelena
Momcilović, Ivana
Grubisic, Dragoljub V
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Asplenium ceterach belongs to a group of poikilohydric ferns and it can recover uninjured from an almost completely dehydrated stale. In our study, short term dehydration (24h) at four different water potentials, resulted in moderate water loss (partial desiccation) in fern tissue. The main phenolic acids represented in A. ceterach were chlorogenic (CGA) and caffeic acid (CA) and their content decreased during the dehydration process. For the first time, peroxidase (POD) and polyphenol oxidase (PPO) isoforms were determined in the rustyback fern. The results exhibit the presence of numerous anionic POD isoforms, with pI ranging from 4.4 to 5.8, but none of the cationic isoforms was detected. Two PPO isofoms were identified, one anionic with pI 6.3 and one cationic with pI of about 9.0. Short-term dehydration brought about a remarkable increase in POD and PPO activity using CGA as a substrate. Changes in enzyme activity and content of substrates during dehydration may play an important ...role in the adaptation of the rustyback fern to water deficit, and increase the overall plant resistance to stress conditions.
Кључне речи:
polyphenol oxidase / peroxidase / dehydration / Asplenium ceterachИзвор:
Archives of Biological Sciences, 2010, 62, 4, 1071-1081Издавач:
- Srpsko biološko društvo, Beograd, i dr.
Финансирање / пројекти:
- Светлосна и хормонална контрола растења и развића биљака, размножавање in vitro и ex situ заштита ретких и угрожених врста (RS-143031)
- Регулација антиоксидативног метаболизма биљака у току растења, инфекције патогенима и деловања абиотичког стреса: механизми транспорта, сигнализације и отпорности (RS-143020)
DOI: 10.2298/ABS1004071Z
ISSN: 0354-4664
WoS: 000287217500025
Scopus: 2-s2.0-78650973907
Институција/група
Institut za multidisciplinarna istraživanjaTY - JOUR AU - Živković, Suzana AU - Popović, M AU - Dragišić Maksimović, Jelena AU - Momcilović, Ivana AU - Grubisic, Dragoljub V PY - 2010 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/377 AB - Asplenium ceterach belongs to a group of poikilohydric ferns and it can recover uninjured from an almost completely dehydrated stale. In our study, short term dehydration (24h) at four different water potentials, resulted in moderate water loss (partial desiccation) in fern tissue. The main phenolic acids represented in A. ceterach were chlorogenic (CGA) and caffeic acid (CA) and their content decreased during the dehydration process. For the first time, peroxidase (POD) and polyphenol oxidase (PPO) isoforms were determined in the rustyback fern. The results exhibit the presence of numerous anionic POD isoforms, with pI ranging from 4.4 to 5.8, but none of the cationic isoforms was detected. Two PPO isofoms were identified, one anionic with pI 6.3 and one cationic with pI of about 9.0. Short-term dehydration brought about a remarkable increase in POD and PPO activity using CGA as a substrate. Changes in enzyme activity and content of substrates during dehydration may play an important role in the adaptation of the rustyback fern to water deficit, and increase the overall plant resistance to stress conditions. PB - Srpsko biološko društvo, Beograd, i dr. T2 - Archives of Biological Sciences T1 - Dehydration-related changes of peroxidase and polyphenol oxidase activity in fronds of the resurrection fern asplenium ceterach l. EP - 1081 IS - 4 SP - 1071 VL - 62 DO - 10.2298/ABS1004071Z ER -
@article{ author = "Živković, Suzana and Popović, M and Dragišić Maksimović, Jelena and Momcilović, Ivana and Grubisic, Dragoljub V", year = "2010", abstract = "Asplenium ceterach belongs to a group of poikilohydric ferns and it can recover uninjured from an almost completely dehydrated stale. In our study, short term dehydration (24h) at four different water potentials, resulted in moderate water loss (partial desiccation) in fern tissue. The main phenolic acids represented in A. ceterach were chlorogenic (CGA) and caffeic acid (CA) and their content decreased during the dehydration process. For the first time, peroxidase (POD) and polyphenol oxidase (PPO) isoforms were determined in the rustyback fern. The results exhibit the presence of numerous anionic POD isoforms, with pI ranging from 4.4 to 5.8, but none of the cationic isoforms was detected. Two PPO isofoms were identified, one anionic with pI 6.3 and one cationic with pI of about 9.0. Short-term dehydration brought about a remarkable increase in POD and PPO activity using CGA as a substrate. Changes in enzyme activity and content of substrates during dehydration may play an important role in the adaptation of the rustyback fern to water deficit, and increase the overall plant resistance to stress conditions.", publisher = "Srpsko biološko društvo, Beograd, i dr.", journal = "Archives of Biological Sciences", title = "Dehydration-related changes of peroxidase and polyphenol oxidase activity in fronds of the resurrection fern asplenium ceterach l.", pages = "1081-1071", number = "4", volume = "62", doi = "10.2298/ABS1004071Z" }
Živković, S., Popović, M., Dragišić Maksimović, J., Momcilović, I.,& Grubisic, D. V.. (2010). Dehydration-related changes of peroxidase and polyphenol oxidase activity in fronds of the resurrection fern asplenium ceterach l.. in Archives of Biological Sciences Srpsko biološko društvo, Beograd, i dr.., 62(4), 1071-1081. https://doi.org/10.2298/ABS1004071Z
Živković S, Popović M, Dragišić Maksimović J, Momcilović I, Grubisic DV. Dehydration-related changes of peroxidase and polyphenol oxidase activity in fronds of the resurrection fern asplenium ceterach l.. in Archives of Biological Sciences. 2010;62(4):1071-1081. doi:10.2298/ABS1004071Z .
Živković, Suzana, Popović, M, Dragišić Maksimović, Jelena, Momcilović, Ivana, Grubisic, Dragoljub V, "Dehydration-related changes of peroxidase and polyphenol oxidase activity in fronds of the resurrection fern asplenium ceterach l." in Archives of Biological Sciences, 62, no. 4 (2010):1071-1081, https://doi.org/10.2298/ABS1004071Z . .