The effects of manganese and copper in vitro and in vivo on peroxidase catalytic cycles
Само за регистроване кориснике
2010
Чланак у часопису (Објављена верзија)

Метаподаци
Приказ свих података о документуАпстракт
Here we present the results of in vitro and in vivo studies of the influence of Mn2+ and Cu2+ on the peroxidative and oxidative catalytic functions of class III peroxidase. Complex peroxidase catalysis by intermediates generated in the reaction was analyzed by utilizing the activating effect of Mn2+ and the inhibitory effect of Cu2+ on the oxidative reaction in vitro. p-Coumaric acid was used as an enzyme substrate in the peroxidative reaction and as a cofactor in the oxidative reaction. In order to correlate the observed in vitro effects with the in vivo situation, we exposed maize plants to excess concentrations of Mn2+ and Cu2+ in the hydroponic solutions. Copper severely arrested plant growth, while manganese exerted no significant effect. The effects on peroxidase activity and isoforms profile of root soluble and cell wall bound fractions were studied. Inhibition of the peroxidase oxidative function by copper was reversible, localized in the cell wall, and accompanied by disappear...ance of some and appearance of new cationic isoforms. Copper-mediated changes were suppressed by the presence of manganese, although Mn2+ treatment per se did not affect the activity of the peroxidase enzyme. The results on the peroxidase activity in maize roots grown with excess Mn2+ and Cu2+ point to the coupling between the oxidative cycle, root growth and different peroxidase isoforms.
Кључне речи:
Peroxidase / p-Coumaric acid / Manganese / Copper / Cell wallИзвор:
Journal of Plant Physiology, 2010, 167, 18, 1550-1557Издавач:
- Elsevier Gmbh, Munich
Финансирање / пројекти:
- Регулација антиоксидативног метаболизма биљака у току растења, инфекције патогенима и деловања абиотичког стреса: механизми транспорта, сигнализације и отпорности (RS-143020)
- Биофизичка истраживања мембранских процеса: интеракција мембранских рецептора и канала са спољашњим факторима и интрацелуларна регулација (RS-143016)
DOI: 10.1016/j.jplph.2010.05.026
ISSN: 0176-1617
PubMed: 20691497
WoS: 000286484000004
Scopus: 2-s2.0-78149413954
Институција/група
Institut za multidisciplinarna istraživanjaTY - JOUR AU - Hadzi-Tasković Sukalović, Vesna AU - Vuletic, Mirjana M AU - Veljović-Jovanović, Sonja AU - Vučinić, Željko PY - 2010 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/373 AB - Here we present the results of in vitro and in vivo studies of the influence of Mn2+ and Cu2+ on the peroxidative and oxidative catalytic functions of class III peroxidase. Complex peroxidase catalysis by intermediates generated in the reaction was analyzed by utilizing the activating effect of Mn2+ and the inhibitory effect of Cu2+ on the oxidative reaction in vitro. p-Coumaric acid was used as an enzyme substrate in the peroxidative reaction and as a cofactor in the oxidative reaction. In order to correlate the observed in vitro effects with the in vivo situation, we exposed maize plants to excess concentrations of Mn2+ and Cu2+ in the hydroponic solutions. Copper severely arrested plant growth, while manganese exerted no significant effect. The effects on peroxidase activity and isoforms profile of root soluble and cell wall bound fractions were studied. Inhibition of the peroxidase oxidative function by copper was reversible, localized in the cell wall, and accompanied by disappearance of some and appearance of new cationic isoforms. Copper-mediated changes were suppressed by the presence of manganese, although Mn2+ treatment per se did not affect the activity of the peroxidase enzyme. The results on the peroxidase activity in maize roots grown with excess Mn2+ and Cu2+ point to the coupling between the oxidative cycle, root growth and different peroxidase isoforms. PB - Elsevier Gmbh, Munich T2 - Journal of Plant Physiology T1 - The effects of manganese and copper in vitro and in vivo on peroxidase catalytic cycles EP - 1557 IS - 18 SP - 1550 VL - 167 DO - 10.1016/j.jplph.2010.05.026 ER -
@article{ author = "Hadzi-Tasković Sukalović, Vesna and Vuletic, Mirjana M and Veljović-Jovanović, Sonja and Vučinić, Željko", year = "2010", abstract = "Here we present the results of in vitro and in vivo studies of the influence of Mn2+ and Cu2+ on the peroxidative and oxidative catalytic functions of class III peroxidase. Complex peroxidase catalysis by intermediates generated in the reaction was analyzed by utilizing the activating effect of Mn2+ and the inhibitory effect of Cu2+ on the oxidative reaction in vitro. p-Coumaric acid was used as an enzyme substrate in the peroxidative reaction and as a cofactor in the oxidative reaction. In order to correlate the observed in vitro effects with the in vivo situation, we exposed maize plants to excess concentrations of Mn2+ and Cu2+ in the hydroponic solutions. Copper severely arrested plant growth, while manganese exerted no significant effect. The effects on peroxidase activity and isoforms profile of root soluble and cell wall bound fractions were studied. Inhibition of the peroxidase oxidative function by copper was reversible, localized in the cell wall, and accompanied by disappearance of some and appearance of new cationic isoforms. Copper-mediated changes were suppressed by the presence of manganese, although Mn2+ treatment per se did not affect the activity of the peroxidase enzyme. The results on the peroxidase activity in maize roots grown with excess Mn2+ and Cu2+ point to the coupling between the oxidative cycle, root growth and different peroxidase isoforms.", publisher = "Elsevier Gmbh, Munich", journal = "Journal of Plant Physiology", title = "The effects of manganese and copper in vitro and in vivo on peroxidase catalytic cycles", pages = "1557-1550", number = "18", volume = "167", doi = "10.1016/j.jplph.2010.05.026" }
Hadzi-Tasković Sukalović, V., Vuletic, M. M., Veljović-Jovanović, S.,& Vučinić, Ž.. (2010). The effects of manganese and copper in vitro and in vivo on peroxidase catalytic cycles. in Journal of Plant Physiology Elsevier Gmbh, Munich., 167(18), 1550-1557. https://doi.org/10.1016/j.jplph.2010.05.026
Hadzi-Tasković Sukalović V, Vuletic MM, Veljović-Jovanović S, Vučinić Ž. The effects of manganese and copper in vitro and in vivo on peroxidase catalytic cycles. in Journal of Plant Physiology. 2010;167(18):1550-1557. doi:10.1016/j.jplph.2010.05.026 .
Hadzi-Tasković Sukalović, Vesna, Vuletic, Mirjana M, Veljović-Jovanović, Sonja, Vučinić, Željko, "The effects of manganese and copper in vitro and in vivo on peroxidase catalytic cycles" in Journal of Plant Physiology, 167, no. 18 (2010):1550-1557, https://doi.org/10.1016/j.jplph.2010.05.026 . .