Nepetalactone content in shoot cultures of three endemic Nepeta species and the evaluation of their antimicrobial activity

Rapid micropropagation of Nepeta rtanjensis, N. sibirica and N. nervosa was performed. Qualitative and quantitative nepetalactone content in methanol extracts of in vitro grown plants was analysed by reverse-phase HPLC coupled with UV and MS detection. Only trans,cis-nepetalactone was detected in shoots of N. rtanjensis, while cis,trans-nepetalactone stereoisomer was present in N. sibirica. No nepetalactone was observed in shoots of N. nervosa. The antimicrobial activity of methanol extracts, against eight bacterial and eight fungal species, was evaluated. All the tested extracts showed significant antibacterial and strong antifungal activity. However, N. rtanjensis extract exhibited the best antimicrobial potential.

Keywords:

Nepetalactone / Nepeta sibirica / Nepeta rtanjensis / Nepeta nervosa / Antimicrobial activity

Source:
Fitoterapia, 2010, 81, 6, 621-626

Publisher:

Elsevier, Amsterdam

Funding / projects:

DOI: 10.1016/j.fitote.2010.03.007

ISSN: 0367-326X

PubMed: 20307630

WoS: 000281073800033

Scopus: 2-s2.0-77955713295

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TY  - JOUR
AU  - Nestorović, Jasmina M
AU  - Misic, Danijela
AU  - Siler, Branislav
AU  - Soković, Marina
AU  - Glamočlija, Jasmina
AU  - Ciric, Ana D
AU  - Maksimović, Vuk
AU  - Grubisic, Dragoljub V
PY  - 2010
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/372
AB  - Rapid micropropagation of Nepeta rtanjensis, N. sibirica and N. nervosa was performed. Qualitative and quantitative nepetalactone content in methanol extracts of in vitro grown plants was analysed by reverse-phase HPLC coupled with UV and MS detection. Only trans,cis-nepetalactone was detected in shoots of N. rtanjensis, while cis,trans-nepetalactone stereoisomer was present in N. sibirica. No nepetalactone was observed in shoots of N. nervosa. The antimicrobial activity of methanol extracts, against eight bacterial and eight fungal species, was evaluated. All the tested extracts showed significant antibacterial and strong antifungal activity. However, N. rtanjensis extract exhibited the best antimicrobial potential.
PB  - Elsevier, Amsterdam
T2  - Fitoterapia
T1  - Nepetalactone content in shoot cultures of three endemic Nepeta species and the evaluation of their antimicrobial activity
EP  - 626
IS  - 6
SP  - 621
VL  - 81
DO  - 10.1016/j.fitote.2010.03.007
ER  -

@article{
author = "Nestorović, Jasmina M and Misic, Danijela and Siler, Branislav and Soković, Marina and Glamočlija, Jasmina and Ciric, Ana D and Maksimović, Vuk and Grubisic, Dragoljub V",
year = "2010",
abstract = "Rapid micropropagation of Nepeta rtanjensis, N. sibirica and N. nervosa was performed. Qualitative and quantitative nepetalactone content in methanol extracts of in vitro grown plants was analysed by reverse-phase HPLC coupled with UV and MS detection. Only trans,cis-nepetalactone was detected in shoots of N. rtanjensis, while cis,trans-nepetalactone stereoisomer was present in N. sibirica. No nepetalactone was observed in shoots of N. nervosa. The antimicrobial activity of methanol extracts, against eight bacterial and eight fungal species, was evaluated. All the tested extracts showed significant antibacterial and strong antifungal activity. However, N. rtanjensis extract exhibited the best antimicrobial potential.",
publisher = "Elsevier, Amsterdam",
journal = "Fitoterapia",
title = "Nepetalactone content in shoot cultures of three endemic Nepeta species and the evaluation of their antimicrobial activity",
pages = "626-621",
number = "6",
volume = "81",
doi = "10.1016/j.fitote.2010.03.007"
}

Nestorović, J. M., Misic, D., Siler, B., Soković, M., Glamočlija, J., Ciric, A. D., Maksimović, V.,& Grubisic, D. V.. (2010). Nepetalactone content in shoot cultures of three endemic Nepeta species and the evaluation of their antimicrobial activity. in Fitoterapia
Elsevier, Amsterdam., 81(6), 621-626.
https://doi.org/10.1016/j.fitote.2010.03.007

Nestorović JM, Misic D, Siler B, Soković M, Glamočlija J, Ciric AD, Maksimović V, Grubisic DV. Nepetalactone content in shoot cultures of three endemic Nepeta species and the evaluation of their antimicrobial activity. in Fitoterapia. 2010;81(6):621-626.
doi:10.1016/j.fitote.2010.03.007 .

Nestorović, Jasmina M, Misic, Danijela, Siler, Branislav, Soković, Marina, Glamočlija, Jasmina, Ciric, Ana D, Maksimović, Vuk, Grubisic, Dragoljub V, "Nepetalactone content in shoot cultures of three endemic Nepeta species and the evaluation of their antimicrobial activity" in Fitoterapia, 81, no. 6 (2010):621-626,
https://doi.org/10.1016/j.fitote.2010.03.007 . .