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EPR Spin-Trapping and Spin-Probing Spectroscopy in Assessing Antioxidant Properties: Example on Extracts of Catkin, Leaves, and Spiny Burs of Castanea sativa
Electron paramagnetic resonance (EPR) spin-trapping and spin-probing techniques were applied to determine antioxidant activity of extracts of catkin, leaves, and spiny burs of Castanea sativa against physiologically relevant reactive species-superoxide and hydroxyl radical generated in simple chemical systems and hydrogen peroxide applied on erythrocytes. Efflux of K+ was used as a marker of membrane integrity. Chemical composition of extracts was analyzed using HPLC/DAD and LC/MS. Extracts showed high antioxidative capacity against superoxide but lower activity against hydroxyl radical. They protected fluidity and integrity of membranes of erythrocytes exposed to hydrogen peroxide. Levels of derivatives of ellagitannins showed positive correlation with the antioxidative activity of extracts. Therefore, ellagitannins from chestnut extracts could represent easily accessible natural antioxidants and beneficial component of human diet in pathophysiological conditions related to oxidative ...stress. In conclusion, EPR spectroscopy represents a valuable tool for evaluation of antioxidant activity in both hydrophilic and lipophilic media.
TY - JOUR
AU - Živković, Jelena
AU - Zeković, Zoran
AU - Mujic, Ibrahim
AU - Godevac, Dejan
AU - Mojović, Miloš
AU - Mujic, Aida
AU - Spasojević, Ivan
PY - 2009
UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/360
AB - Electron paramagnetic resonance (EPR) spin-trapping and spin-probing techniques were applied to determine antioxidant activity of extracts of catkin, leaves, and spiny burs of Castanea sativa against physiologically relevant reactive species-superoxide and hydroxyl radical generated in simple chemical systems and hydrogen peroxide applied on erythrocytes. Efflux of K+ was used as a marker of membrane integrity. Chemical composition of extracts was analyzed using HPLC/DAD and LC/MS. Extracts showed high antioxidative capacity against superoxide but lower activity against hydroxyl radical. They protected fluidity and integrity of membranes of erythrocytes exposed to hydrogen peroxide. Levels of derivatives of ellagitannins showed positive correlation with the antioxidative activity of extracts. Therefore, ellagitannins from chestnut extracts could represent easily accessible natural antioxidants and beneficial component of human diet in pathophysiological conditions related to oxidative stress. In conclusion, EPR spectroscopy represents a valuable tool for evaluation of antioxidant activity in both hydrophilic and lipophilic media.
PB - Springer, New York
T2 - Food Biophysics
T1 - EPR Spin-Trapping and Spin-Probing Spectroscopy in Assessing Antioxidant Properties: Example on Extracts of Catkin, Leaves, and Spiny Burs of Castanea sativa
EP - 133
IS - 2
SP - 126
VL - 4
DO - 10.1007/s11483-009-9109-8
ER -
@article{
author = "Živković, Jelena and Zeković, Zoran and Mujic, Ibrahim and Godevac, Dejan and Mojović, Miloš and Mujic, Aida and Spasojević, Ivan",
year = "2009",
abstract = "Electron paramagnetic resonance (EPR) spin-trapping and spin-probing techniques were applied to determine antioxidant activity of extracts of catkin, leaves, and spiny burs of Castanea sativa against physiologically relevant reactive species-superoxide and hydroxyl radical generated in simple chemical systems and hydrogen peroxide applied on erythrocytes. Efflux of K+ was used as a marker of membrane integrity. Chemical composition of extracts was analyzed using HPLC/DAD and LC/MS. Extracts showed high antioxidative capacity against superoxide but lower activity against hydroxyl radical. They protected fluidity and integrity of membranes of erythrocytes exposed to hydrogen peroxide. Levels of derivatives of ellagitannins showed positive correlation with the antioxidative activity of extracts. Therefore, ellagitannins from chestnut extracts could represent easily accessible natural antioxidants and beneficial component of human diet in pathophysiological conditions related to oxidative stress. In conclusion, EPR spectroscopy represents a valuable tool for evaluation of antioxidant activity in both hydrophilic and lipophilic media.",
publisher = "Springer, New York",
journal = "Food Biophysics",
title = "EPR Spin-Trapping and Spin-Probing Spectroscopy in Assessing Antioxidant Properties: Example on Extracts of Catkin, Leaves, and Spiny Burs of Castanea sativa",
pages = "133-126",
number = "2",
volume = "4",
doi = "10.1007/s11483-009-9109-8"
}
Živković, J., Zeković, Z., Mujic, I., Godevac, D., Mojović, M., Mujic, A.,& Spasojević, I.. (2009). EPR Spin-Trapping and Spin-Probing Spectroscopy in Assessing Antioxidant Properties: Example on Extracts of Catkin, Leaves, and Spiny Burs of Castanea sativa. in Food Biophysics
Springer, New York., 4(2), 126-133.
https://doi.org/10.1007/s11483-009-9109-8
Živković J, Zeković Z, Mujic I, Godevac D, Mojović M, Mujic A, Spasojević I. EPR Spin-Trapping and Spin-Probing Spectroscopy in Assessing Antioxidant Properties: Example on Extracts of Catkin, Leaves, and Spiny Burs of Castanea sativa. in Food Biophysics. 2009;4(2):126-133.
doi:10.1007/s11483-009-9109-8 .
Živković, Jelena, Zeković, Zoran, Mujic, Ibrahim, Godevac, Dejan, Mojović, Miloš, Mujic, Aida, Spasojević, Ivan, "EPR Spin-Trapping and Spin-Probing Spectroscopy in Assessing Antioxidant Properties: Example on Extracts of Catkin, Leaves, and Spiny Burs of Castanea sativa" in Food Biophysics, 4, no. 2 (2009):126-133,
https://doi.org/10.1007/s11483-009-9109-8 . .
