Occurrence and Distribution of Grapevine Yellows Caused by Stolbur Phytoplasma in Montenegro
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Radonjic, SanjaHrncic, Snjezana

Jović, Jelena

Cvrković, Tatjana

Krstic, Oliver

Krnjajić, Slobodan

Tosevski, Ivo

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In August 2008, grapevine yellows (GY)-like symptoms were observed in vineyards near Podgorica, Montenegro. A survey of the grape growing area of Montenegro was conducted to identify and determine the distribution of phytoplasmas associated with grapevine. Phytoplasmas were detected in symptomatic vine plants using PCR with universal primers for amplification of the phytoplasma 16S rRNA gene. From the 42 plants sampled, 36 were positive for the presence of phytoplasmas. RFLP profiles of the 16S rDNA of all phytoplasma positive isolates tested indicated that they belong to 16SrXII-A phylogenetic group. PCR with stolbur specific primers confirmed the presence of stolbur phytoplasma in grapevine samples from Montenegro. Sequence and RFLP analysis of the tuf gene indicated the presence of the tufAY-a and tufAY-b types of stolbur phytoplasma. To our knowledge, this is the first report of phytoplasma infection of grapevine in Montenegro.
Keywords:
stolbur phytoplasma / RFLP / PCR / Montenegro / grapevine yellows / Bois noirSource:
Journal of Phytopathology, 2009, 157, 11-12, 682-685Publisher:
- Wiley, Hoboken
Funding / projects:
- SEE-ERA.NET Pilot Joint [9608]
DOI: 10.1111/j.1439-0434.2009.01560.x
ISSN: 0931-1785
WoS: 000271493800005
Scopus: 2-s2.0-71049160356
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Institut za multidisciplinarna istraživanjaTY - JOUR AU - Radonjic, Sanja AU - Hrncic, Snjezana AU - Jović, Jelena AU - Cvrković, Tatjana AU - Krstic, Oliver AU - Krnjajić, Slobodan AU - Tosevski, Ivo PY - 2009 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/332 AB - In August 2008, grapevine yellows (GY)-like symptoms were observed in vineyards near Podgorica, Montenegro. A survey of the grape growing area of Montenegro was conducted to identify and determine the distribution of phytoplasmas associated with grapevine. Phytoplasmas were detected in symptomatic vine plants using PCR with universal primers for amplification of the phytoplasma 16S rRNA gene. From the 42 plants sampled, 36 were positive for the presence of phytoplasmas. RFLP profiles of the 16S rDNA of all phytoplasma positive isolates tested indicated that they belong to 16SrXII-A phylogenetic group. PCR with stolbur specific primers confirmed the presence of stolbur phytoplasma in grapevine samples from Montenegro. Sequence and RFLP analysis of the tuf gene indicated the presence of the tufAY-a and tufAY-b types of stolbur phytoplasma. To our knowledge, this is the first report of phytoplasma infection of grapevine in Montenegro. PB - Wiley, Hoboken T2 - Journal of Phytopathology T1 - Occurrence and Distribution of Grapevine Yellows Caused by Stolbur Phytoplasma in Montenegro EP - 685 IS - 11-12 SP - 682 VL - 157 DO - 10.1111/j.1439-0434.2009.01560.x ER -
@article{ author = "Radonjic, Sanja and Hrncic, Snjezana and Jović, Jelena and Cvrković, Tatjana and Krstic, Oliver and Krnjajić, Slobodan and Tosevski, Ivo", year = "2009", abstract = "In August 2008, grapevine yellows (GY)-like symptoms were observed in vineyards near Podgorica, Montenegro. A survey of the grape growing area of Montenegro was conducted to identify and determine the distribution of phytoplasmas associated with grapevine. Phytoplasmas were detected in symptomatic vine plants using PCR with universal primers for amplification of the phytoplasma 16S rRNA gene. From the 42 plants sampled, 36 were positive for the presence of phytoplasmas. RFLP profiles of the 16S rDNA of all phytoplasma positive isolates tested indicated that they belong to 16SrXII-A phylogenetic group. PCR with stolbur specific primers confirmed the presence of stolbur phytoplasma in grapevine samples from Montenegro. Sequence and RFLP analysis of the tuf gene indicated the presence of the tufAY-a and tufAY-b types of stolbur phytoplasma. To our knowledge, this is the first report of phytoplasma infection of grapevine in Montenegro.", publisher = "Wiley, Hoboken", journal = "Journal of Phytopathology", title = "Occurrence and Distribution of Grapevine Yellows Caused by Stolbur Phytoplasma in Montenegro", pages = "685-682", number = "11-12", volume = "157", doi = "10.1111/j.1439-0434.2009.01560.x" }
Radonjic, S., Hrncic, S., Jović, J., Cvrković, T., Krstic, O., Krnjajić, S.,& Tosevski, I.. (2009). Occurrence and Distribution of Grapevine Yellows Caused by Stolbur Phytoplasma in Montenegro. in Journal of Phytopathology Wiley, Hoboken., 157(11-12), 682-685. https://doi.org/10.1111/j.1439-0434.2009.01560.x
Radonjic S, Hrncic S, Jović J, Cvrković T, Krstic O, Krnjajić S, Tosevski I. Occurrence and Distribution of Grapevine Yellows Caused by Stolbur Phytoplasma in Montenegro. in Journal of Phytopathology. 2009;157(11-12):682-685. doi:10.1111/j.1439-0434.2009.01560.x .
Radonjic, Sanja, Hrncic, Snjezana, Jović, Jelena, Cvrković, Tatjana, Krstic, Oliver, Krnjajić, Slobodan, Tosevski, Ivo, "Occurrence and Distribution of Grapevine Yellows Caused by Stolbur Phytoplasma in Montenegro" in Journal of Phytopathology, 157, no. 11-12 (2009):682-685, https://doi.org/10.1111/j.1439-0434.2009.01560.x . .