The aim of this study was to investigate the relationship between antioxidant enzymes and reactive oxygen species production in diapausing larvae of the European corn borer, Ostrinia nubilalis (Lepidoptera: Pyralidae) kept at 5 degrees C, -3 degrees C and -16 degrees C for two weeks. The amount of hydrogen peroxide (H2O2), activity of antioxidant enzymes, copper zinc superoxide dismutase (CuZnSOD), manganese superoxide dismutases (MnSOD) and catalase (CAT) in whole body homogenates, as well as the electron paramagnetic resonance (EPR) spectroscopy of this insect's whole body were analysed. A higher level of melanin radical and lower CuZnSOD and CAT activities were found in larvae kept at -3 degrees C than at 5 degrees C and -16 degrees C. At the same temperature (-3 degrees C) an elevated H2O2 concentration was recorded. A possible regulatory role of H2O2 at -3 degrees C, which is the temperature that triggers freezing tolerance, is suggested.
TY - JOUR
AU - Kojic, Danijela
AU - Spasojević, Ivan
AU - Mojović, Miloš
AU - Blagojević, Duško
AU - Worland, M. Roger
AU - Grubor-Lajsic, Gordana
AU - Spasić, Mihajlo
PY - 2009
UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/328
AB - The aim of this study was to investigate the relationship between antioxidant enzymes and reactive oxygen species production in diapausing larvae of the European corn borer, Ostrinia nubilalis (Lepidoptera: Pyralidae) kept at 5 degrees C, -3 degrees C and -16 degrees C for two weeks. The amount of hydrogen peroxide (H2O2), activity of antioxidant enzymes, copper zinc superoxide dismutase (CuZnSOD), manganese superoxide dismutases (MnSOD) and catalase (CAT) in whole body homogenates, as well as the electron paramagnetic resonance (EPR) spectroscopy of this insect's whole body were analysed. A higher level of melanin radical and lower CuZnSOD and CAT activities were found in larvae kept at -3 degrees C than at 5 degrees C and -16 degrees C. At the same temperature (-3 degrees C) an elevated H2O2 concentration was recorded. A possible regulatory role of H2O2 at -3 degrees C, which is the temperature that triggers freezing tolerance, is suggested.
PB - Czech Acad Sci, Inst Entomology, Ceske Budejovice
T2 - European Journal of Entomology
T1 - Potential role of hydrogen peroxide and melanin in the cold hardiness of Ostrinia nubilalis (Lepidoptera: Pyralidae)
EP - 454
IS - 3
SP - 451
VL - 106
DO - 10.14411/eje.2009.056
ER -
@article{
author = "Kojic, Danijela and Spasojević, Ivan and Mojović, Miloš and Blagojević, Duško and Worland, M. Roger and Grubor-Lajsic, Gordana and Spasić, Mihajlo",
year = "2009",
abstract = "The aim of this study was to investigate the relationship between antioxidant enzymes and reactive oxygen species production in diapausing larvae of the European corn borer, Ostrinia nubilalis (Lepidoptera: Pyralidae) kept at 5 degrees C, -3 degrees C and -16 degrees C for two weeks. The amount of hydrogen peroxide (H2O2), activity of antioxidant enzymes, copper zinc superoxide dismutase (CuZnSOD), manganese superoxide dismutases (MnSOD) and catalase (CAT) in whole body homogenates, as well as the electron paramagnetic resonance (EPR) spectroscopy of this insect's whole body were analysed. A higher level of melanin radical and lower CuZnSOD and CAT activities were found in larvae kept at -3 degrees C than at 5 degrees C and -16 degrees C. At the same temperature (-3 degrees C) an elevated H2O2 concentration was recorded. A possible regulatory role of H2O2 at -3 degrees C, which is the temperature that triggers freezing tolerance, is suggested.",
publisher = "Czech Acad Sci, Inst Entomology, Ceske Budejovice",
journal = "European Journal of Entomology",
title = "Potential role of hydrogen peroxide and melanin in the cold hardiness of Ostrinia nubilalis (Lepidoptera: Pyralidae)",
pages = "454-451",
number = "3",
volume = "106",
doi = "10.14411/eje.2009.056"
}
Kojic, D., Spasojević, I., Mojović, M., Blagojević, D., Worland, M. R., Grubor-Lajsic, G.,& Spasić, M.. (2009). Potential role of hydrogen peroxide and melanin in the cold hardiness of Ostrinia nubilalis (Lepidoptera: Pyralidae). in European Journal of Entomology
Czech Acad Sci, Inst Entomology, Ceske Budejovice., 106(3), 451-454.
https://doi.org/10.14411/eje.2009.056
Kojic D, Spasojević I, Mojović M, Blagojević D, Worland MR, Grubor-Lajsic G, Spasić M. Potential role of hydrogen peroxide and melanin in the cold hardiness of Ostrinia nubilalis (Lepidoptera: Pyralidae). in European Journal of Entomology. 2009;106(3):451-454.
doi:10.14411/eje.2009.056 .
Kojic, Danijela, Spasojević, Ivan, Mojović, Miloš, Blagojević, Duško, Worland, M. Roger, Grubor-Lajsic, Gordana, Spasić, Mihajlo, "Potential role of hydrogen peroxide and melanin in the cold hardiness of Ostrinia nubilalis (Lepidoptera: Pyralidae)" in European Journal of Entomology, 106, no. 3 (2009):451-454,
https://doi.org/10.14411/eje.2009.056 . .
