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Relevance of the capacity of phosphorylated fructose to scavenge the hydroxyl radical

Authorized Users Only
2009
Authors
Spasojević, Ivan
Mojović, Miloš
Blagojević, Duško
Spasic, Snežana D.
Jones, David R
Nikolić-Kokić, Aleksandra
Spasić, Mihajlo
Article (Published version)
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Abstract
The hydroxyl radical ((OH)-O-center dot) has detrimental biological activity due to its very high reactivity. Our experiments were designed to determine the effects of equimolar concentrations of glucose, fructose and mannitol and three phosphorylated forms of fructose (fructose-1-phosphate (F1P): fructose-6-phosphate (F6P): and fructose-1,6-bis(phosphate) (F16BP)) on (OH)-O-center dot radical production via the Fenton reaction. EPR spectroscopy using spin-trap DEPMPO was applied to detect radical production. We found that the percentage inhibition of (OH)-O-center dot radical formation decreased in the order F16BP > F1P > F6P > fructose > mannitol = glucose. As ketoses can sequester redox-active iron thus preventing the Fenton reaction, the Haber-Weiss-like system was also employed to generate (OH)-O-center dot, so that the effect of iron sequestration could be distinguished from direct (OH)-O-center dot radical scavenging. In the latter system, the rank order of (OH)-O-center dot sca...venging activity was F16BP > F1P > F6P > fructose = mannitol = glucose. Our results clearly demonstrate that intracellular phosphorylated forms of fructose have more scavenging properties than fructose or glucose, leading us to conclude that the acute administration of fructose could overcome the body's reaction to exogenous antioxidants during appropriate therapy in certain pathophysiological conditions related to oxidative stress, Such as sepsis, neurodegenerative diseases, atherosclerosis, malignancy, and some complications of pregnancy.

Keywords:
Mannitol / Hydroxyl radical / Haber-Weiss reaction / Glucose / Fructose / EPR spectroscopy
Source:
Carbohydrate Research, 2009, 344, 1, 80-84
Publisher:
  • Elsevier Sci Ltd, Oxford
Funding / projects:
  • The Role of Redox-Active Substances in the Maintenance of Homeostasis (RS-143034)
  • Biofizička istraživanja membranskih procesa: interakcija membranskih receptora i kanala sa spoljašnjim faktorima i intracelularna regulacija (RS-143016)

DOI: 10.1016/j.carres.2008.09.025

ISSN: 0008-6215

PubMed: 18947823

WoS: 000262596200011

Scopus: 2-s2.0-57749111966
[ Google Scholar ]
52
45
URI
http://rimsi.imsi.bg.ac.rs/handle/123456789/321
Collections
  • Radovi istraživača / Researchers’ publications
Institution/Community
Institut za multidisciplinarna istraživanja
TY  - JOUR
AU  - Spasojević, Ivan
AU  - Mojović, Miloš
AU  - Blagojević, Duško
AU  - Spasic, Snežana D.
AU  - Jones, David R
AU  - Nikolić-Kokić, Aleksandra
AU  - Spasić, Mihajlo
PY  - 2009
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/321
AB  - The hydroxyl radical ((OH)-O-center dot) has detrimental biological activity due to its very high reactivity. Our experiments were designed to determine the effects of equimolar concentrations of glucose, fructose and mannitol and three phosphorylated forms of fructose (fructose-1-phosphate (F1P): fructose-6-phosphate (F6P): and fructose-1,6-bis(phosphate) (F16BP)) on (OH)-O-center dot radical production via the Fenton reaction. EPR spectroscopy using spin-trap DEPMPO was applied to detect radical production. We found that the percentage inhibition of (OH)-O-center dot radical formation decreased in the order F16BP > F1P > F6P > fructose > mannitol = glucose. As ketoses can sequester redox-active iron thus preventing the Fenton reaction, the Haber-Weiss-like system was also employed to generate (OH)-O-center dot, so that the effect of iron sequestration could be distinguished from direct (OH)-O-center dot radical scavenging. In the latter system, the rank order of (OH)-O-center dot scavenging activity was F16BP > F1P > F6P > fructose = mannitol = glucose. Our results clearly demonstrate that intracellular phosphorylated forms of fructose have more scavenging properties than fructose or glucose, leading us to conclude that the acute administration of fructose could overcome the body's reaction to exogenous antioxidants during appropriate therapy in certain pathophysiological conditions related to oxidative stress, Such as sepsis, neurodegenerative diseases, atherosclerosis, malignancy, and some complications of pregnancy.
PB  - Elsevier Sci Ltd, Oxford
T2  - Carbohydrate Research
T1  - Relevance of the capacity of phosphorylated fructose to scavenge the hydroxyl radical
EP  - 84
IS  - 1
SP  - 80
VL  - 344
DO  - 10.1016/j.carres.2008.09.025
ER  - 
@article{
author = "Spasojević, Ivan and Mojović, Miloš and Blagojević, Duško and Spasic, Snežana D. and Jones, David R and Nikolić-Kokić, Aleksandra and Spasić, Mihajlo",
year = "2009",
abstract = "The hydroxyl radical ((OH)-O-center dot) has detrimental biological activity due to its very high reactivity. Our experiments were designed to determine the effects of equimolar concentrations of glucose, fructose and mannitol and three phosphorylated forms of fructose (fructose-1-phosphate (F1P): fructose-6-phosphate (F6P): and fructose-1,6-bis(phosphate) (F16BP)) on (OH)-O-center dot radical production via the Fenton reaction. EPR spectroscopy using spin-trap DEPMPO was applied to detect radical production. We found that the percentage inhibition of (OH)-O-center dot radical formation decreased in the order F16BP > F1P > F6P > fructose > mannitol = glucose. As ketoses can sequester redox-active iron thus preventing the Fenton reaction, the Haber-Weiss-like system was also employed to generate (OH)-O-center dot, so that the effect of iron sequestration could be distinguished from direct (OH)-O-center dot radical scavenging. In the latter system, the rank order of (OH)-O-center dot scavenging activity was F16BP > F1P > F6P > fructose = mannitol = glucose. Our results clearly demonstrate that intracellular phosphorylated forms of fructose have more scavenging properties than fructose or glucose, leading us to conclude that the acute administration of fructose could overcome the body's reaction to exogenous antioxidants during appropriate therapy in certain pathophysiological conditions related to oxidative stress, Such as sepsis, neurodegenerative diseases, atherosclerosis, malignancy, and some complications of pregnancy.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Carbohydrate Research",
title = "Relevance of the capacity of phosphorylated fructose to scavenge the hydroxyl radical",
pages = "84-80",
number = "1",
volume = "344",
doi = "10.1016/j.carres.2008.09.025"
}
Spasojević, I., Mojović, M., Blagojević, D., Spasic, S. D., Jones, D. R., Nikolić-Kokić, A.,& Spasić, M.. (2009). Relevance of the capacity of phosphorylated fructose to scavenge the hydroxyl radical. in Carbohydrate Research
Elsevier Sci Ltd, Oxford., 344(1), 80-84.
https://doi.org/10.1016/j.carres.2008.09.025
Spasojević I, Mojović M, Blagojević D, Spasic SD, Jones DR, Nikolić-Kokić A, Spasić M. Relevance of the capacity of phosphorylated fructose to scavenge the hydroxyl radical. in Carbohydrate Research. 2009;344(1):80-84.
doi:10.1016/j.carres.2008.09.025 .
Spasojević, Ivan, Mojović, Miloš, Blagojević, Duško, Spasic, Snežana D., Jones, David R, Nikolić-Kokić, Aleksandra, Spasić, Mihajlo, "Relevance of the capacity of phosphorylated fructose to scavenge the hydroxyl radical" in Carbohydrate Research, 344, no. 1 (2009):80-84,
https://doi.org/10.1016/j.carres.2008.09.025 . .

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