Assessment of growth-promoting properties of Pseudomonas spp. on soybeans under field conditions
Authors
Jelušić, AleksandraJanakiev, Tamara
Unković, Nikola
Ljaljević Grbić, Milica
Degrassi, Giuliano
Dimkić, Ivica
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The soybean is recognized worldwide as one of the most important crops due to its protein- and
oil-rich seeds. Several beneficial bacterial strains, including those of the genus Pseudomonas, are
known to increase plant yield and disease tolerance. The aim of this study was to test the potential of 15 strains of Pseudomonas spp. to promote soybean growth under field conditions. Strains were identified based on 16S rRNA and gyrB sequences, and strains belonging to risk group 1 were further analyzed. Selected strains were tested for plant growth-promoting (PGP) properties, biofilm formation and in vitro antimicrobial activity against various fungal pathogens. PGP activity indicators of the selected strains were evaluated on soybean plants grown in fertilizer-free soil and in soil treated with 70% and 100% fertilizers. Three Pseudomonas spp. strains, P. putida VB131A, P. fulva VB134B and P. rhodesiae VB143S, were selected as PGP candidates. All three strains showed the ability to produce... IAA and solubilize phosphorus, while only P. rhodesiae VB143S showed strong lipase and protease activity. Only P. fulva VB134B showed the ability to
produce biofilm and hydrogen cyanide. All three strains showed in vitro antifungal activity against
Alternaria infectoria. P. fulva VB134B and P. rhodesiae VB143S showed antifungal activity against
Alternaria alternata and P. putida VB131A and P. rhodesiae VB143S against Epicoccum nigrum. Only P. fulva VB134B showed activity against Monilinia laxa. Statistically significant differences in
plant height were not observed regardless of the strain or soil treatment used. In terms of first
pod height, the P. fulva VB134B treatment was most effective when applied to soybeans grown
in 100% fertilized soil. In contrast, plants treated with P. rhodesiae VB143S and grown in 100% fertilized soil had the highest number of branches and pods per plant.
Keywords:
Pseudomonas spp. / soybean / plant growth-promoting propertiesSource:
XIII CONGRESS OF MICROBIOLOGISTS OF SERBIA with international participation, UMS 24 - MIKROMED REGIO 5 "From biotechnology to human and planetary health", 2024, 70-70Publisher:
- Serbian Society for Microbiology
Funding / projects:
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200178 (University of Belgrade, Faculty of Biology) (RS-MESTD-inst-2020-200178)
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200053 (University of Belgrade, Institute for Multidisciplinary Research) (RS-MESTD-inst-2020-200053)
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Institut za multidisciplinarna istraživanjaTY - CONF AU - Jelušić, Aleksandra AU - Janakiev, Tamara AU - Unković, Nikola AU - Ljaljević Grbić, Milica AU - Degrassi, Giuliano AU - Dimkić, Ivica PY - 2024 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/3205 AB - The soybean is recognized worldwide as one of the most important crops due to its protein- and oil-rich seeds. Several beneficial bacterial strains, including those of the genus Pseudomonas, are known to increase plant yield and disease tolerance. The aim of this study was to test the potential of 15 strains of Pseudomonas spp. to promote soybean growth under field conditions. Strains were identified based on 16S rRNA and gyrB sequences, and strains belonging to risk group 1 were further analyzed. Selected strains were tested for plant growth-promoting (PGP) properties, biofilm formation and in vitro antimicrobial activity against various fungal pathogens. PGP activity indicators of the selected strains were evaluated on soybean plants grown in fertilizer-free soil and in soil treated with 70% and 100% fertilizers. Three Pseudomonas spp. strains, P. putida VB131A, P. fulva VB134B and P. rhodesiae VB143S, were selected as PGP candidates. All three strains showed the ability to produce IAA and solubilize phosphorus, while only P. rhodesiae VB143S showed strong lipase and protease activity. Only P. fulva VB134B showed the ability to produce biofilm and hydrogen cyanide. All three strains showed in vitro antifungal activity against Alternaria infectoria. P. fulva VB134B and P. rhodesiae VB143S showed antifungal activity against Alternaria alternata and P. putida VB131A and P. rhodesiae VB143S against Epicoccum nigrum. Only P. fulva VB134B showed activity against Monilinia laxa. Statistically significant differences in plant height were not observed regardless of the strain or soil treatment used. In terms of first pod height, the P. fulva VB134B treatment was most effective when applied to soybeans grown in 100% fertilized soil. In contrast, plants treated with P. rhodesiae VB143S and grown in 100% fertilized soil had the highest number of branches and pods per plant. PB - Serbian Society for Microbiology C3 - XIII CONGRESS OF MICROBIOLOGISTS OF SERBIA with international participation, UMS 24 - MIKROMED REGIO 5 "From biotechnology to human and planetary health" T1 - Assessment of growth-promoting properties of Pseudomonas spp. on soybeans under field conditions EP - 70 SP - 70 UR - https://hdl.handle.net/21.15107/rcub_rimsi_3205 ER -
@conference{ author = "Jelušić, Aleksandra and Janakiev, Tamara and Unković, Nikola and Ljaljević Grbić, Milica and Degrassi, Giuliano and Dimkić, Ivica", year = "2024", abstract = "The soybean is recognized worldwide as one of the most important crops due to its protein- and oil-rich seeds. Several beneficial bacterial strains, including those of the genus Pseudomonas, are known to increase plant yield and disease tolerance. The aim of this study was to test the potential of 15 strains of Pseudomonas spp. to promote soybean growth under field conditions. Strains were identified based on 16S rRNA and gyrB sequences, and strains belonging to risk group 1 were further analyzed. Selected strains were tested for plant growth-promoting (PGP) properties, biofilm formation and in vitro antimicrobial activity against various fungal pathogens. PGP activity indicators of the selected strains were evaluated on soybean plants grown in fertilizer-free soil and in soil treated with 70% and 100% fertilizers. Three Pseudomonas spp. strains, P. putida VB131A, P. fulva VB134B and P. rhodesiae VB143S, were selected as PGP candidates. All three strains showed the ability to produce IAA and solubilize phosphorus, while only P. rhodesiae VB143S showed strong lipase and protease activity. Only P. fulva VB134B showed the ability to produce biofilm and hydrogen cyanide. All three strains showed in vitro antifungal activity against Alternaria infectoria. P. fulva VB134B and P. rhodesiae VB143S showed antifungal activity against Alternaria alternata and P. putida VB131A and P. rhodesiae VB143S against Epicoccum nigrum. Only P. fulva VB134B showed activity against Monilinia laxa. Statistically significant differences in plant height were not observed regardless of the strain or soil treatment used. In terms of first pod height, the P. fulva VB134B treatment was most effective when applied to soybeans grown in 100% fertilized soil. In contrast, plants treated with P. rhodesiae VB143S and grown in 100% fertilized soil had the highest number of branches and pods per plant.", publisher = "Serbian Society for Microbiology", journal = "XIII CONGRESS OF MICROBIOLOGISTS OF SERBIA with international participation, UMS 24 - MIKROMED REGIO 5 "From biotechnology to human and planetary health"", title = "Assessment of growth-promoting properties of Pseudomonas spp. on soybeans under field conditions", pages = "70-70", url = "https://hdl.handle.net/21.15107/rcub_rimsi_3205" }
Jelušić, A., Janakiev, T., Unković, N., Ljaljević Grbić, M., Degrassi, G.,& Dimkić, I.. (2024). Assessment of growth-promoting properties of Pseudomonas spp. on soybeans under field conditions. in XIII CONGRESS OF MICROBIOLOGISTS OF SERBIA with international participation, UMS 24 - MIKROMED REGIO 5 "From biotechnology to human and planetary health" Serbian Society for Microbiology., 70-70. https://hdl.handle.net/21.15107/rcub_rimsi_3205
Jelušić A, Janakiev T, Unković N, Ljaljević Grbić M, Degrassi G, Dimkić I. Assessment of growth-promoting properties of Pseudomonas spp. on soybeans under field conditions. in XIII CONGRESS OF MICROBIOLOGISTS OF SERBIA with international participation, UMS 24 - MIKROMED REGIO 5 "From biotechnology to human and planetary health". 2024;:70-70. https://hdl.handle.net/21.15107/rcub_rimsi_3205 .
Jelušić, Aleksandra, Janakiev, Tamara, Unković, Nikola, Ljaljević Grbić, Milica, Degrassi, Giuliano, Dimkić, Ivica, "Assessment of growth-promoting properties of Pseudomonas spp. on soybeans under field conditions" in XIII CONGRESS OF MICROBIOLOGISTS OF SERBIA with international participation, UMS 24 - MIKROMED REGIO 5 "From biotechnology to human and planetary health" (2024):70-70, https://hdl.handle.net/21.15107/rcub_rimsi_3205 .