Characterisation of Mung bean (Vigna radiata L.) seeds using fluorescence spectroscopy and multivariate analyis
Апстракт
Mung bean (Vigna radiata L.) is a leguminous plant cultivated mainly in south-east Asia and used as an ingredient in local cuisine. Its principal nutritional value is contained in its constituents such as starch, proteins, (poly)phenols, and natural antioxidants. Fluorescence spectroscopy is increasingly used as a method of choice for food analysis; due to the presence of different fluorophores originating from aromatic amino acids and secondary metabolites, it is useful for proteins and phenolics detection. In this study, the total protein and phenolic contents of mung bean seed extracts were determined using the Bradford method and Folin–Ciocalteu (FC) reagent, respectively. Antioxidant activity was determined using DPPH (2,2-Diphenyl-1-picrylhydrazyl) assay. Fluorescence spectra were recorded for a series of excitation-emission wavelengths. Further, we used the multivariate analysis on the recorded excitation-emission fluorescence matrix of the studied samples. The results showed th...e presence of three different fluorescence components, with the position of the emission maximum corresponding to the fluorophore of proteins (component 1 with excitation/emission peak maxima at Ex 290/Em 345 nm) and phenolics (component 2 - Ex 295/Em 395 nm and component 3 - Ex 350/Em 450 nm). This fluorescence-based method could be a useful approach for estimating the nutrient properties of leguminous food.
Кључне речи:
Mung bean / fluorescence spectroscopy / MCR-ALS / TPC / DPPHИзвор:
Book of Proceedings: XIV International Scientific Agriculture Symposium "AGROSYM 2023", October 05-08, 2023, Jahorina, BiH, 2023, 299-303Издавач:
- University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200053 (Универзитет у Београду, Институт за мултидисциплинарна истраживања) (RS-MESTD-inst-2020-200053)
Институција/група
Institut za multidisciplinarna istraživanjaTY - CONF AU - Prokopijević, Miloš AU - Bartolić, Dragana AU - Stanković, Mira AU - Radotić, Ksenija PY - 2023 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/3134 AB - Mung bean (Vigna radiata L.) is a leguminous plant cultivated mainly in south-east Asia and used as an ingredient in local cuisine. Its principal nutritional value is contained in its constituents such as starch, proteins, (poly)phenols, and natural antioxidants. Fluorescence spectroscopy is increasingly used as a method of choice for food analysis; due to the presence of different fluorophores originating from aromatic amino acids and secondary metabolites, it is useful for proteins and phenolics detection. In this study, the total protein and phenolic contents of mung bean seed extracts were determined using the Bradford method and Folin–Ciocalteu (FC) reagent, respectively. Antioxidant activity was determined using DPPH (2,2-Diphenyl-1-picrylhydrazyl) assay. Fluorescence spectra were recorded for a series of excitation-emission wavelengths. Further, we used the multivariate analysis on the recorded excitation-emission fluorescence matrix of the studied samples. The results showed the presence of three different fluorescence components, with the position of the emission maximum corresponding to the fluorophore of proteins (component 1 with excitation/emission peak maxima at Ex 290/Em 345 nm) and phenolics (component 2 - Ex 295/Em 395 nm and component 3 - Ex 350/Em 450 nm). This fluorescence-based method could be a useful approach for estimating the nutrient properties of leguminous food. PB - University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia C3 - Book of Proceedings: XIV International Scientific Agriculture Symposium "AGROSYM 2023", October 05-08, 2023, Jahorina, BiH T1 - Characterisation of Mung bean (Vigna radiata L.) seeds using fluorescence spectroscopy and multivariate analyis EP - 303 SP - 299 UR - https://hdl.handle.net/21.15107/rcub_rimsi_3134 ER -
@conference{ author = "Prokopijević, Miloš and Bartolić, Dragana and Stanković, Mira and Radotić, Ksenija", year = "2023", abstract = "Mung bean (Vigna radiata L.) is a leguminous plant cultivated mainly in south-east Asia and used as an ingredient in local cuisine. Its principal nutritional value is contained in its constituents such as starch, proteins, (poly)phenols, and natural antioxidants. Fluorescence spectroscopy is increasingly used as a method of choice for food analysis; due to the presence of different fluorophores originating from aromatic amino acids and secondary metabolites, it is useful for proteins and phenolics detection. In this study, the total protein and phenolic contents of mung bean seed extracts were determined using the Bradford method and Folin–Ciocalteu (FC) reagent, respectively. Antioxidant activity was determined using DPPH (2,2-Diphenyl-1-picrylhydrazyl) assay. Fluorescence spectra were recorded for a series of excitation-emission wavelengths. Further, we used the multivariate analysis on the recorded excitation-emission fluorescence matrix of the studied samples. The results showed the presence of three different fluorescence components, with the position of the emission maximum corresponding to the fluorophore of proteins (component 1 with excitation/emission peak maxima at Ex 290/Em 345 nm) and phenolics (component 2 - Ex 295/Em 395 nm and component 3 - Ex 350/Em 450 nm). This fluorescence-based method could be a useful approach for estimating the nutrient properties of leguminous food.", publisher = "University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia", journal = "Book of Proceedings: XIV International Scientific Agriculture Symposium "AGROSYM 2023", October 05-08, 2023, Jahorina, BiH", title = "Characterisation of Mung bean (Vigna radiata L.) seeds using fluorescence spectroscopy and multivariate analyis", pages = "303-299", url = "https://hdl.handle.net/21.15107/rcub_rimsi_3134" }
Prokopijević, M., Bartolić, D., Stanković, M.,& Radotić, K.. (2023). Characterisation of Mung bean (Vigna radiata L.) seeds using fluorescence spectroscopy and multivariate analyis. in Book of Proceedings: XIV International Scientific Agriculture Symposium "AGROSYM 2023", October 05-08, 2023, Jahorina, BiH University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia., 299-303. https://hdl.handle.net/21.15107/rcub_rimsi_3134
Prokopijević M, Bartolić D, Stanković M, Radotić K. Characterisation of Mung bean (Vigna radiata L.) seeds using fluorescence spectroscopy and multivariate analyis. in Book of Proceedings: XIV International Scientific Agriculture Symposium "AGROSYM 2023", October 05-08, 2023, Jahorina, BiH. 2023;:299-303. https://hdl.handle.net/21.15107/rcub_rimsi_3134 .
Prokopijević, Miloš, Bartolić, Dragana, Stanković, Mira, Radotić, Ksenija, "Characterisation of Mung bean (Vigna radiata L.) seeds using fluorescence spectroscopy and multivariate analyis" in Book of Proceedings: XIV International Scientific Agriculture Symposium "AGROSYM 2023", October 05-08, 2023, Jahorina, BiH (2023):299-303, https://hdl.handle.net/21.15107/rcub_rimsi_3134 .