MODIFICATION OF CARBOXYMETHYLCELLULOSE WITH PHENOLS FOR PEROXIDASE INDUCED HYDROGELS FORMATION AND ELECTROSPINNING
Аутори
Popović, NikolinaProdanović, Olivera
Gadjanski, Ivana
Cvetković, Danijela
Živanović, Marko
Pavlović, Vladimir
Filipović, Nenad D.
Prodanović, Radivoje
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Carboxymethylcellulose (CMC) is water-soluble cellulose ether which is used in food and cosmetics industry. It also
has big potential for use in pharmaceutical products due to its high biocompatibility, biodegradibility, low immunogenicity and
low price. Crosslinked CMC can absorb large amounts of water and swell to form hydrogels with great physical properties.
The need for new biomaterials and hydrogels is growing daily, due to their use in tissue engineering, drug delivery and cell
and enzyme immobilization studies. In this study we modified CMC, in order to get a crosslinkable polymer that can make
hydrogels by chemical and enzymatic means. After periodate oxidation of CMC we obtained CMC with different degrees of
oxidation: 2.5, 5, 10, 15 and 20 mol%. Further modification using reductive amination in the presence of different phenolic
compounds like tyramine, was done. This modification of CMC was confirmed by UV–VIS and FT-IR spectroscopy, while
concentration of phenol an...d ionizable groups was determined using absorbance at 275 nm and acid–base titration. All CMCtyramines
were able to form hydrogels after cross-linking with horse radish peroxidase (HRP) and hydrogen peroxide. CMC
derivatives have been successfully electrospun and crosslinked afterwards. Due to the introduction of amino groups and
decrease in molecular weight, they were significantly more soluble in water up to 30 % (w/w) compared to native
polysaccharides and their electrospinability also improved. We aim to make nanofibers using tyramine-polysaccharides that
will be more stable in cell culture media after cross-linking covalently and with calcium/barium ions. Diameter of nanofibers
was determined by scanning electron microscopy (SEM). Cross-linked nanofibers that we obtained will be used for tissue
engineering of blood vessels.
Кључне речи:
Carboxymethylcellulose / hydrogels / nanofibersИзвор:
The Annual International Conference Romanian Society for Biochemistry & Molecular Biology, 8 - 9. June, Temišvar, 2017, 26, 2, S4_P10-Издавач:
- West University of Timișoara
Финансирање / пројекти:
- Испитивања односа структура-функција у ћелијском зиду биљака и измене структуре зида ензимским инжењерингом (RS-MESTD-Basic Research (BR or ON)-173017)
- Алергени, антитела, ензими и мали физиолошки значајни молекули: дизајн, структура, функција и значај (RS-MESTD-Basic Research (BR or ON)-172049)
Институција/група
Institut za multidisciplinarna istraživanjaTY - CONF AU - Popović, Nikolina AU - Prodanović, Olivera AU - Gadjanski, Ivana AU - Cvetković, Danijela AU - Živanović, Marko AU - Pavlović, Vladimir AU - Filipović, Nenad D. AU - Prodanović, Radivoje PY - 2017 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/3123 AB - Carboxymethylcellulose (CMC) is water-soluble cellulose ether which is used in food and cosmetics industry. It also has big potential for use in pharmaceutical products due to its high biocompatibility, biodegradibility, low immunogenicity and low price. Crosslinked CMC can absorb large amounts of water and swell to form hydrogels with great physical properties. The need for new biomaterials and hydrogels is growing daily, due to their use in tissue engineering, drug delivery and cell and enzyme immobilization studies. In this study we modified CMC, in order to get a crosslinkable polymer that can make hydrogels by chemical and enzymatic means. After periodate oxidation of CMC we obtained CMC with different degrees of oxidation: 2.5, 5, 10, 15 and 20 mol%. Further modification using reductive amination in the presence of different phenolic compounds like tyramine, was done. This modification of CMC was confirmed by UV–VIS and FT-IR spectroscopy, while concentration of phenol and ionizable groups was determined using absorbance at 275 nm and acid–base titration. All CMCtyramines were able to form hydrogels after cross-linking with horse radish peroxidase (HRP) and hydrogen peroxide. CMC derivatives have been successfully electrospun and crosslinked afterwards. Due to the introduction of amino groups and decrease in molecular weight, they were significantly more soluble in water up to 30 % (w/w) compared to native polysaccharides and their electrospinability also improved. We aim to make nanofibers using tyramine-polysaccharides that will be more stable in cell culture media after cross-linking covalently and with calcium/barium ions. Diameter of nanofibers was determined by scanning electron microscopy (SEM). Cross-linked nanofibers that we obtained will be used for tissue engineering of blood vessels. PB - West University of Timișoara C3 - The Annual International Conference Romanian Society for Biochemistry & Molecular Biology, 8 - 9. June, Temišvar T1 - MODIFICATION OF CARBOXYMETHYLCELLULOSE WITH PHENOLS FOR PEROXIDASE INDUCED HYDROGELS FORMATION AND ELECTROSPINNING IS - 2 SP - S4_P10 VL - 26 UR - https://hdl.handle.net/21.15107/rcub_rimsi_3123 ER -
@conference{ author = "Popović, Nikolina and Prodanović, Olivera and Gadjanski, Ivana and Cvetković, Danijela and Živanović, Marko and Pavlović, Vladimir and Filipović, Nenad D. and Prodanović, Radivoje", year = "2017", abstract = "Carboxymethylcellulose (CMC) is water-soluble cellulose ether which is used in food and cosmetics industry. It also has big potential for use in pharmaceutical products due to its high biocompatibility, biodegradibility, low immunogenicity and low price. Crosslinked CMC can absorb large amounts of water and swell to form hydrogels with great physical properties. The need for new biomaterials and hydrogels is growing daily, due to their use in tissue engineering, drug delivery and cell and enzyme immobilization studies. In this study we modified CMC, in order to get a crosslinkable polymer that can make hydrogels by chemical and enzymatic means. After periodate oxidation of CMC we obtained CMC with different degrees of oxidation: 2.5, 5, 10, 15 and 20 mol%. Further modification using reductive amination in the presence of different phenolic compounds like tyramine, was done. This modification of CMC was confirmed by UV–VIS and FT-IR spectroscopy, while concentration of phenol and ionizable groups was determined using absorbance at 275 nm and acid–base titration. All CMCtyramines were able to form hydrogels after cross-linking with horse radish peroxidase (HRP) and hydrogen peroxide. CMC derivatives have been successfully electrospun and crosslinked afterwards. Due to the introduction of amino groups and decrease in molecular weight, they were significantly more soluble in water up to 30 % (w/w) compared to native polysaccharides and their electrospinability also improved. We aim to make nanofibers using tyramine-polysaccharides that will be more stable in cell culture media after cross-linking covalently and with calcium/barium ions. Diameter of nanofibers was determined by scanning electron microscopy (SEM). Cross-linked nanofibers that we obtained will be used for tissue engineering of blood vessels.", publisher = "West University of Timișoara", journal = "The Annual International Conference Romanian Society for Biochemistry & Molecular Biology, 8 - 9. June, Temišvar", title = "MODIFICATION OF CARBOXYMETHYLCELLULOSE WITH PHENOLS FOR PEROXIDASE INDUCED HYDROGELS FORMATION AND ELECTROSPINNING", number = "2", pages = "S4_P10", volume = "26", url = "https://hdl.handle.net/21.15107/rcub_rimsi_3123" }
Popović, N., Prodanović, O., Gadjanski, I., Cvetković, D., Živanović, M., Pavlović, V., Filipović, N. D.,& Prodanović, R.. (2017). MODIFICATION OF CARBOXYMETHYLCELLULOSE WITH PHENOLS FOR PEROXIDASE INDUCED HYDROGELS FORMATION AND ELECTROSPINNING. in The Annual International Conference Romanian Society for Biochemistry & Molecular Biology, 8 - 9. June, Temišvar West University of Timișoara., 26(2), S4_P10. https://hdl.handle.net/21.15107/rcub_rimsi_3123
Popović N, Prodanović O, Gadjanski I, Cvetković D, Živanović M, Pavlović V, Filipović ND, Prodanović R. MODIFICATION OF CARBOXYMETHYLCELLULOSE WITH PHENOLS FOR PEROXIDASE INDUCED HYDROGELS FORMATION AND ELECTROSPINNING. in The Annual International Conference Romanian Society for Biochemistry & Molecular Biology, 8 - 9. June, Temišvar. 2017;26(2):S4_P10. https://hdl.handle.net/21.15107/rcub_rimsi_3123 .
Popović, Nikolina, Prodanović, Olivera, Gadjanski, Ivana, Cvetković, Danijela, Živanović, Marko, Pavlović, Vladimir, Filipović, Nenad D., Prodanović, Radivoje, "MODIFICATION OF CARBOXYMETHYLCELLULOSE WITH PHENOLS FOR PEROXIDASE INDUCED HYDROGELS FORMATION AND ELECTROSPINNING" in The Annual International Conference Romanian Society for Biochemistry & Molecular Biology, 8 - 9. June, Temišvar, 26, no. 2 (2017):S4_P10, https://hdl.handle.net/21.15107/rcub_rimsi_3123 .