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dc.creatorKalicanin, Nevena
dc.creatorBalaz, Ana Marija
dc.creatorProdanović, Olivera
dc.creatorProdanović, Radivoje
dc.date.accessioned2023-12-12T08:56:21Z
dc.date.available2023-12-12T08:56:21Z
dc.date.issued2022
dc.identifier.isbn978-86-7220-124-6 (FOC)
dc.identifier.urihttp://rimsi.imsi.bg.ac.rs/handle/123456789/3048
dc.description.abstractOpine dehydrogenases are a family of NAD(P)H dependent oxidoreductases, which catalyze the reductive condensation of an α amino group from an amino acid with an αketo acid during anaerobic glycolysis by regenerating NAD. They are widespread in cephalopods and mollusks. Opines are associated with crown gall tumor pathogenesis caused by A. tumefaciens providing nutrients to the pathogen, and novel opine compounds acting as metallophores have been identified. Besides, opine-type secondary amine dicarboxylic acids are chiral intermediates of angiotensin-converting enzyme inhibitors. A novel enzyme originating from an extremophile bacterium, with assumed opine dehydrogenase function was successfully expressed in Escherichia coli STAR cells and purified by affinity chromatography. Molecular mass determined by SDS-PAGE was approximately 40 kDa. The activity was measured by using pyruvate and alanine as substrates, by which proved that it has opine dehydrogenase activity.sr
dc.language.isoensr
dc.publisherFaculty of Chemistry, Serbian Biochemical Societysr
dc.relationinfo:eu-repo/grantAgreement/MESTD/inst-2020/200053/RS//sr
dc.relationinfo:eu-repo/grantAgreement/MESTD/inst-2020/200168/RS//sr
dc.relationinfo:eu-repo/grantAgreement/MESTD/inst-2020/200026/RS//sr
dc.rightsopenAccesssr
dc.sourceProceedings Eleventh Conference, Scientific meeting of an international character "Amazing Biochemistry"sr
dc.subjectOpine dehydrogenasessr
dc.titleProduction of a novel opine dehydrogenasesr
dc.typeconferenceObjectsr
dc.rights.licenseARRsr
dc.citation.spage79
dc.identifier.fulltexthttp://rimsi.imsi.bg.ac.rs/bitstream/id/7942/bitstream_7942.pdf
dc.identifier.rcubhttps://hdl.handle.net/21.15107/rcub_rimsi_3048
dc.type.versionpublishedVersionsr


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