Dopamine-modified pectins for laccase induced hydrogel formation and immobilization
Samo za registrovane korisnike
2019
Konferencijski prilog (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Pectins belong to a group of a plant polysaccharides that are structural components of plant
cell walls. These polysaccharides have big potential for use in the food industry as a
gelling agents and biomedical application due their biocompatibility, biodegradability, low
price, etc. Chemical modifications of pectin are useful methods for introducing various
functional groups, which give pectin hydrogels novel properties. In this study, pectin was
modified by oxidation with sodium periodate at molar ratios of 5, 10 and 15 mol% and
reductive amination with dopamine and sodium cyanoborohydride afterwards. This
modification of pectin was confirmed by UV-VIS and NMR spectroscopy. Dopaminepectins showed gelling properties in the presence of laccase and oxygen. These pectin
derivatives were tested as carriers for laccase immobilization within microbeads formed in
an emulsion based enzymatic polymerization reaction. Laccase was expressed in functional
form in E. coli, isolated and ad...ditionally purified. We determined optimal conditions for
immobilization andobtained microbeads with specific activity of 0.0006 IU/mg and 40%
yield of immobilization. The immobilized laccase could be used for various application,
such as decolorization of textile dyes
.
Ključne reči:
Pectin / laccase / hydrogelIzvor:
The 9th conference of the Serbian Biochemical Society: Diversity in Biochemistry, 2019, 168-168Izdavač:
- Serbian Biochemical Society
Finansiranje / projekti:
- Alergeni, antitela, enzimi i mali fiziološki značajni molekuli: dizajn, struktura, funkcija i značaj (RS-MESTD-Basic Research (BR or ON)-172049)
- Razvoj novih inkapsulacionih i enzimskih tehnologija za proizvodnju biokatalizatora i biološki aktivnih komponenata hrane u cilju povećanja njene konkurentnosti, kvaliteta i bezbednosti (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46010)
Institucija/grupa
Institut za multidisciplinarna istraživanjaTY - CONF AU - Stanišić, Marija AU - Popović, Nikolina AU - Prodanović, Olivera AU - Prodanović, Radivoje PY - 2019 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/2912 AB - Pectins belong to a group of a plant polysaccharides that are structural components of plant cell walls. These polysaccharides have big potential for use in the food industry as a gelling agents and biomedical application due their biocompatibility, biodegradability, low price, etc. Chemical modifications of pectin are useful methods for introducing various functional groups, which give pectin hydrogels novel properties. In this study, pectin was modified by oxidation with sodium periodate at molar ratios of 5, 10 and 15 mol% and reductive amination with dopamine and sodium cyanoborohydride afterwards. This modification of pectin was confirmed by UV-VIS and NMR spectroscopy. Dopaminepectins showed gelling properties in the presence of laccase and oxygen. These pectin derivatives were tested as carriers for laccase immobilization within microbeads formed in an emulsion based enzymatic polymerization reaction. Laccase was expressed in functional form in E. coli, isolated and additionally purified. We determined optimal conditions for immobilization andobtained microbeads with specific activity of 0.0006 IU/mg and 40% yield of immobilization. The immobilized laccase could be used for various application, such as decolorization of textile dyes . PB - Serbian Biochemical Society C3 - The 9th conference of the Serbian Biochemical Society: Diversity in Biochemistry T1 - Dopamine-modified pectins for laccase induced hydrogel formation and immobilization EP - 168 SP - 168 UR - https://hdl.handle.net/21.15107/rcub_rimsi_2912 ER -
@conference{ author = "Stanišić, Marija and Popović, Nikolina and Prodanović, Olivera and Prodanović, Radivoje", year = "2019", abstract = "Pectins belong to a group of a plant polysaccharides that are structural components of plant cell walls. These polysaccharides have big potential for use in the food industry as a gelling agents and biomedical application due their biocompatibility, biodegradability, low price, etc. Chemical modifications of pectin are useful methods for introducing various functional groups, which give pectin hydrogels novel properties. In this study, pectin was modified by oxidation with sodium periodate at molar ratios of 5, 10 and 15 mol% and reductive amination with dopamine and sodium cyanoborohydride afterwards. This modification of pectin was confirmed by UV-VIS and NMR spectroscopy. Dopaminepectins showed gelling properties in the presence of laccase and oxygen. These pectin derivatives were tested as carriers for laccase immobilization within microbeads formed in an emulsion based enzymatic polymerization reaction. Laccase was expressed in functional form in E. coli, isolated and additionally purified. We determined optimal conditions for immobilization andobtained microbeads with specific activity of 0.0006 IU/mg and 40% yield of immobilization. The immobilized laccase could be used for various application, such as decolorization of textile dyes .", publisher = "Serbian Biochemical Society", journal = "The 9th conference of the Serbian Biochemical Society: Diversity in Biochemistry", title = "Dopamine-modified pectins for laccase induced hydrogel formation and immobilization", pages = "168-168", url = "https://hdl.handle.net/21.15107/rcub_rimsi_2912" }
Stanišić, M., Popović, N., Prodanović, O.,& Prodanović, R.. (2019). Dopamine-modified pectins for laccase induced hydrogel formation and immobilization. in The 9th conference of the Serbian Biochemical Society: Diversity in Biochemistry Serbian Biochemical Society., 168-168. https://hdl.handle.net/21.15107/rcub_rimsi_2912
Stanišić M, Popović N, Prodanović O, Prodanović R. Dopamine-modified pectins for laccase induced hydrogel formation and immobilization. in The 9th conference of the Serbian Biochemical Society: Diversity in Biochemistry. 2019;:168-168. https://hdl.handle.net/21.15107/rcub_rimsi_2912 .
Stanišić, Marija, Popović, Nikolina, Prodanović, Olivera, Prodanović, Radivoje, "Dopamine-modified pectins for laccase induced hydrogel formation and immobilization" in The 9th conference of the Serbian Biochemical Society: Diversity in Biochemistry (2019):168-168, https://hdl.handle.net/21.15107/rcub_rimsi_2912 .